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Strain identifier

BacDive ID: 161945

Type strain: Yes

Species: Micromonospora caldifontis

Strain Designation: HSS6-8

Strain history: C. Thawai HSS6-8.

NCBI tax ID(s): 765196 (species)

For citation purpose refer to the digital object identifier (doi) of the current version.
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@ref: 67770

BacDive-ID: 161945

keywords: 16S sequence, Bacteria, aerobe, spore-forming, mesophilic, Gram-positive

description: Micromonospora caldifontis HSS6-8 is an aerobe, spore-forming, mesophilic bacterium that was isolated from Hot spring soil at Ratchaburi Province.

NCBI tax id

  • NCBI tax id: 765196
  • Matching level: species

strain history

  • @ref: 67770
  • history: C. Thawai HSS6-8.

doi: 10.13145/bacdive161945.20230509.8

Name and taxonomic classification


  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Micromonosporales
  • family: Micromonosporaceae
  • genus: Micromonospora
  • species: Micromonospora caldifontis
  • full scientific name: Micromonospora caldifontis Thawai et al. 2019

@ref: 67770

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Micromonosporales

family: Micromonosporaceae

genus: Micromonospora

species: Micromonospora caldifontis

full scientific name: Micromonospora caldifontis Thawai et al. 2019

strain designation: HSS6-8

type strain: yes


cell morphology

  • @ref: 67872
  • gram stain: positive

colony morphology

  • @ref: 67872
  • colony color: pale orange and became transparent orange with age
  • medium used: ISP 2

multicellular morphology

  • @ref: 67872
  • forms multicellular complex: yes
  • medium name: ISP 2
  • further description: form a single spore borne on the substrate mycelium. Aerial mycelium is absent.

Culture and growth conditions

culture temp


culture pH

  • @ref: 67872
  • ability: positive
  • type: growth
  • pH: 5-10
  • PH range: alkaliphile

Physiology and metabolism

oxygen tolerance

  • @ref: 67872
  • oxygen tolerance: aerobe

spore formation

  • @ref: 67872
  • spore description: form a single spore borne on the substrate mycelium. The spore surface is smooth.
  • type of spore: spore
  • spore formation: yes


  • @ref: 67872
  • salt: NaCl
  • growth: positive
  • tested relation: growth
  • concentration: 0-3 %


  • @ref: 67770
  • observation: quinones: MK-10(H6), MK-10(H4)

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6787265327D-xylose+builds acid from
6787216988D-ribose+builds acid from
6787230849L-arabinose+builds acid from
6787215824D-fructose+builds acid from
6787217057cellobiose+builds acid from
6787217634D-glucose-builds acid from
6787212936D-galactose-builds acid from
6787217992sucrose-builds acid from
6787228053melibiose-builds acid from
6787217814salicin-builds acid from
6787217716lactose-builds acid from
6787216899D-mannitol-builds acid from
6787217754glycerol-builds acid from
6787216634raffinose-builds acid from
6787215963ribitol-builds acid from
6787217151xylitol-builds acid from
6787252071dextran-builds acid from
67872DL-2-gamma-aminobutyrate+nitrogen source
6787216467L-arginine+nitrogen source
6787217203L-proline+nitrogen source
6787217115L-serine+nitrogen source
6787224741hydroxyproline-nitrogen source
6787217196L-asparagine-nitrogen source
6787217561L-cysteine-nitrogen source
6787215971L-histidine-nitrogen source
6787216643L-methionine-nitrogen source
6787217295L-phenylalanine-nitrogen source
6787216857L-threonine-nitrogen source
6787216414L-valine-nitrogen source


67872cytochrome oxidase-

Isolation, sampling and environmental information


@refsample typecountryorigin.countrycontinentgeographic locationenrichment cultureenrichment culture compositionenrichment culture durationenrichment culture temperatureisolation procedure
67770Hot spring soil at Ratchaburi ProvinceThailandTHAAsia
67872hot spring soil sampleThailandTHAAsiaRatchaburi provincestarch casein nitrate agar(SCA; l-1: 0.3 g casein, 10 g soluble starch, 2 g KNO3, 2 g NaCl, 2 g K2HPO4, 0.05 g MgSO4 x 7H2O, 0.02 g CaCO3, 0.01 g FeSO4 x 7H2O, 15 g agar; pH 7.2) supplemented with 25 mg l-1 nalidixic acid and 100 mg l-1 nystatin21 days40The soil sample was air-dried at room temperature for 3 days. The dried soil sample (10 g) was added to sterile distilled water (90 ml) then heated at 70°C for 15 min. The treated soil was serially diluted to 10-3 concentration in 0.01 % sterile Tween 80 in distilled water and spread on starch casein nitrate agar. The isolation plate was incubated at 40°C for 21 days.

Sequence information

16S sequences

  • @ref: 67872
  • description: Micromonospora sp. HSS6-8 gene for 16S ribosomal RNA, partial sequence
  • accession: AB560969
  • length: 1411
  • database: ena
  • NCBI tax ID: 765196

GC content

  • @ref: 67872
  • GC-content: 70.5
  • method: high performance liquid chromatography (HPLC)

External links

@ref: 67770

culture collection no.: JCM 17126, TBRC 8927


  • topic: Phylogeny
  • Pubmed-ID: 30810517
  • title: Micromonospora caldifontis sp. nov., isolated from hot spring soil.
  • authors: Thawai C, Tanasupawat S, Kudo T
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijsem.0.003321
  • year: 2019
  • mesh: Bacterial Typing Techniques, Base Composition, Cell Wall/chemistry, DNA, Bacterial/genetics, Diaminopimelic Acid/chemistry, Fatty Acids/chemistry, Hot Springs/*microbiology, Micromonospora/*classification/isolation & purification, Nucleic Acid Hybridization, Peptidoglycan/chemistry, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, *Soil Microbiology, Thailand, Vitamin K 2/chemistry
  • topic2: Transcriptome


20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
67770Curators of the JCM
67872Chitti Thawai, Somboon Tanasupawat, Takuji KudoMicromonospora caldifontis sp. nov., isolated from hot spring soil10.1099/ijsem.0.003321IJSEM 69: 1336-1342 2019