Strain identifier
BacDive ID: 161945
Type strain: 
Species: Micromonospora caldifontis
Strain Designation: HSS6-8
Strain history: C. Thawai HSS6-8.
NCBI tax ID(s): 765196 (species)
version 8 (current version)
General
@ref: 67770
BacDive-ID: 161945
keywords: 16S sequence, Bacteria, aerobe, spore-forming, mesophilic, Gram-positive
description: Micromonospora caldifontis HSS6-8 is an aerobe, spore-forming, mesophilic bacterium that was isolated from Hot spring soil at Ratchaburi Province.
NCBI tax id
- NCBI tax id: 765196
- Matching level: species
strain history
- @ref: 67770
- history: C. Thawai HSS6-8.
doi: 10.13145/bacdive161945.20230509.8
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/actinomycetota
- domain: Bacteria
- phylum: Actinomycetota
- class: Actinomycetes
- order: Micromonosporales
- family: Micromonosporaceae
- genus: Micromonospora
- species: Micromonospora caldifontis
- full scientific name: Micromonospora caldifontis Thawai et al. 2019
@ref: 67770
domain: Bacteria
phylum: Actinobacteria
class: Actinobacteria
order: Micromonosporales
family: Micromonosporaceae
genus: Micromonospora
species: Micromonospora caldifontis
full scientific name: Micromonospora caldifontis Thawai et al. 2019
strain designation: HSS6-8
type strain: yes
Morphology
cell morphology
- @ref: 67872
- gram stain: positive
colony morphology
- @ref: 67872
- colony color: pale orange and became transparent orange with age
- medium used: ISP 2
multicellular morphology
- @ref: 67872
- forms multicellular complex: yes
- medium name: ISP 2
- further description: form a single spore borne on the substrate mycelium. Aerial mycelium is absent.
Culture and growth conditions
culture temp
| @ref | growth | type | temperature | range |
|---|---|---|---|---|
| 67770 | positive | growth | 37 | mesophilic |
| 67872 | positive | growth | 20-48 | |
| 67872 | positive | optimum | 40 | thermophilic |
culture pH
- @ref: 67872
- ability: positive
- type: growth
- pH: 5-10
- PH range: alkaliphile
Physiology and metabolism
oxygen tolerance
- @ref: 67872
- oxygen tolerance: aerobe
spore formation
- @ref: 67872
- spore description: form a single spore borne on the substrate mycelium. The spore surface is smooth.
- type of spore: spore
- spore formation: yes
halophily
- @ref: 67872
- salt: NaCl
- growth: positive
- tested relation: growth
- concentration: 0-3 %
observation
- @ref: 67770
- observation: quinones: MK-10(H6), MK-10(H4)
metabolite utilization
| @ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
|---|---|---|---|---|
| 67872 | 17632 | nitrate | + | reduction |
| 67872 | milk | + | assimilation | |
| 67872 | 28017 | starch | + | hydrolysis |
| 67872 | 18186 | tyrosine | + | hydrolysis |
| 67872 | 16708 | adenine | - | hydrolysis |
| 67872 | 85146 | carboxymethylcellulose | - | hydrolysis |
| 67872 | 17368 | hypoxanthine | - | hydrolysis |
| 67872 | 15318 | xanthine | - | hydrolysis |
| 67872 | 5291 | gelatin | - | hydrolysis |
| 67872 | 65327 | D-xylose | + | builds acid from |
| 67872 | 16988 | D-ribose | + | builds acid from |
| 67872 | 30849 | L-arabinose | + | builds acid from |
| 67872 | 15824 | D-fructose | + | builds acid from |
| 67872 | 17057 | cellobiose | + | builds acid from |
| 67872 | 17634 | D-glucose | - | builds acid from |
| 67872 | 12936 | D-galactose | - | builds acid from |
| 67872 | 17992 | sucrose | - | builds acid from |
| 67872 | 28053 | melibiose | - | builds acid from |
| 67872 | 17814 | salicin | - | builds acid from |
| 67872 | 17716 | lactose | - | builds acid from |
| 67872 | 16899 | D-mannitol | - | builds acid from |
| 67872 | 17754 | glycerol | - | builds acid from |
| 67872 | 16634 | raffinose | - | builds acid from |
| 67872 | 15963 | ribitol | - | builds acid from |
| 67872 | 17151 | xylitol | - | builds acid from |
| 67872 | 52071 | dextran | - | builds acid from |
| 67872 | DL-2-gamma-aminobutyrate | + | nitrogen source | |
| 67872 | 16467 | L-arginine | + | nitrogen source |
| 67872 | 17203 | L-proline | + | nitrogen source |
| 67872 | 17115 | L-serine | + | nitrogen source |
| 67872 | 24741 | hydroxyproline | - | nitrogen source |
| 67872 | 17196 | L-asparagine | - | nitrogen source |
| 67872 | 17561 | L-cysteine | - | nitrogen source |
| 67872 | 15971 | L-histidine | - | nitrogen source |
| 67872 | 16643 | L-methionine | - | nitrogen source |
| 67872 | 17295 | L-phenylalanine | - | nitrogen source |
| 67872 | 16857 | L-threonine | - | nitrogen source |
| 67872 | 16414 | L-valine | - | nitrogen source |
enzymes
| @ref | value | activity | ec |
|---|---|---|---|
| 67872 | catalase | + | 1.11.1.6 |
| 67872 | cytochrome oxidase | - | 1.9.3.1 |
| 67872 | urease | + | 3.5.1.5 |
Isolation, sampling and environmental information
isolation
| @ref | sample type | country | origin.country | continent | geographic location | enrichment culture | enrichment culture composition | enrichment culture duration | enrichment culture temperature | isolation procedure |
|---|---|---|---|---|---|---|---|---|---|---|
| 67770 | Hot spring soil at Ratchaburi Province | Thailand | THA | Asia | ||||||
| 67872 | hot spring soil sample | Thailand | THA | Asia | Ratchaburi province | starch casein nitrate agar | (SCA; l-1: 0.3 g casein, 10 g soluble starch, 2 g KNO3, 2 g NaCl, 2 g K2HPO4, 0.05 g MgSO4 x 7H2O, 0.02 g CaCO3, 0.01 g FeSO4 x 7H2O, 15 g agar; pH 7.2) supplemented with 25 mg l-1 nalidixic acid and 100 mg l-1 nystatin | 21 days | 40 | The soil sample was air-dried at room temperature for 3 days. The dried soil sample (10 g) was added to sterile distilled water (90 ml) then heated at 70°C for 15 min. The treated soil was serially diluted to 10-3 concentration in 0.01 % sterile Tween 80 in distilled water and spread on starch casein nitrate agar. The isolation plate was incubated at 40°C for 21 days. |
Sequence information
16S sequences
- @ref: 67872
- description: Micromonospora sp. HSS6-8 gene for 16S ribosomal RNA, partial sequence
- accession: AB560969
- length: 1411
- database: ena
- NCBI tax ID: 765196
GC content
- @ref: 67872
- GC-content: 70.5
- method: high performance liquid chromatography (HPLC)
External links
@ref: 67770
culture collection no.: JCM 17126, TBRC 8927
literature
- topic: Phylogeny
- Pubmed-ID: 30810517
- title: Micromonospora caldifontis sp. nov., isolated from hot spring soil.
- authors: Thawai C, Tanasupawat S, Kudo T
- journal: Int J Syst Evol Microbiol
- DOI: 10.1099/ijsem.0.003321
- year: 2019
- mesh: Bacterial Typing Techniques, Base Composition, Cell Wall/chemistry, DNA, Bacterial/genetics, Diaminopimelic Acid/chemistry, Fatty Acids/chemistry, Hot Springs/*microbiology, Micromonospora/*classification/isolation & purification, Nucleic Acid Hybridization, Peptidoglycan/chemistry, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, *Soil Microbiology, Thailand, Vitamin K 2/chemistry
- topic2: Transcriptome
Reference
| @id | authors | title | doi/url | journal |
|---|---|---|---|---|
| 20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | 10.1099/ijsem.0.004332 | |
| 67770 | Curators of the JCM | https://jcm.brc.riken.jp/en/ | ||
| 67872 | Chitti Thawai, Somboon Tanasupawat, Takuji Kudo | Micromonospora caldifontis sp. nov., isolated from hot spring soil | 10.1099/ijsem.0.003321 | IJSEM 69: 1336-1342 2019 |