Strain identifier
BacDive ID: 16189
Type strain:
Species: Streptomyces calvus
Strain history: INMI 16.
NCBI tax ID(s): 67282 (species)
General
@ref: 10316
BacDive-ID: 16189
DSM-Number: 41452
keywords: genome sequence, 16S sequence, Bacteria, spore-forming, mesophilic
description: Streptomyces calvus DSM 41452 is a spore-forming, mesophilic bacterium that was isolated from Soil.
NCBI tax id
- NCBI tax id: 67282
- Matching level: species
strain history
@ref | history |
---|---|
10316 | <- N.S. Agre, VKM |
67770 | INMI 16. |
doi: 10.13145/bacdive16189.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/actinomycetota
- domain: Bacteria
- phylum: Actinomycetota
- class: Actinomycetes
- order: Kitasatosporales
- family: Streptomycetaceae
- genus: Streptomyces
- species: Streptomyces calvus
- full scientific name: Streptomyces calvus Backus et al. 1957 (Approved Lists 1980)
synonyms
@ref synonym 20215 Streptomyces asterosporus 20215 Streptomyces aureorectus
@ref: 10316
domain: Bacteria
phylum: Actinobacteria
class: Actinobacteria
order: Actinomycetales
family: Streptomycetaceae
genus: Streptomyces
species: Streptomyces asterosporus
full scientific name: Streptomyces asterosporus (ex Krassilnikov 1970) Preobrazhenskaya 1986
type strain: yes
Morphology
cell morphology
@ref | motility | confidence | gram stain |
---|---|---|---|
69480 | no | 91.821 | |
69480 | 100 | positive |
colony morphology
@ref | colony color | incubation period | medium used |
---|---|---|---|
18687 | Sand yellow (1002) | 10-14 days | ISP 2 |
18687 | Sand yellow (1002) | 10-14 days | ISP 3 |
18687 | Sand yellow (1002) | 10-14 days | ISP 4 |
18687 | Sand yellow (1002) | 10-14 days | ISP 5 |
18687 | Sand yellow (1002) | 10-14 days | ISP 6 |
18687 | Sand yellow (1002) | 10-14 days | ISP 7 |
multicellular morphology
@ref | forms multicellular complex | medium name |
---|---|---|
18687 | no | ISP 2 |
18687 | no | ISP 3 |
18687 | no | ISP 4 |
18687 | no | ISP 5 |
18687 | no | ISP 6 |
18687 | no | ISP 7 |
Culture and growth conditions
culture medium
@ref | name | growth | link | composition |
---|---|---|---|---|
10316 | GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) | yes | https://mediadive.dsmz.de/medium/65 | Name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) Composition: Agar 18.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water |
18687 | ISP 2 | yes | Name: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes | |
18687 | ISP 3 | yes | Name: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms: | |
18687 | ISP 4 | yes | Name: ISP 4; DSM 547 Solution I: Difco soluble starch, 10.0 g. Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml. Solution II: CaCO3 2.0 g K2HPO4 (anhydrous) 1.0 g MgSO4 x 7 H2O 1.0 g NaCl 1.0 g (NH4)2SO4 2.0 g Distilled water 500.0 ml Trace salt solution (see below) 1.0 ml The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min. Trace element solution: FeSO4 x 7 H2O 0.1 g MnCl2 x 4 H2O 0.1 g ZnSO4 x 7 H2O 0.1 g Distilled water 100.0 ml | |
18687 | ISP 5 | yes | Name: ISP 5 (5323) Composition L-Asparagine 1.0 g/l Glycerol 10.0 g/l K2HPO4 1.0 g/l Salt solution (see preparation) 1.0 ml/l Agar 20.0 g/l Preparation: Salt solution 1.0 g FeSO4 x 7 H2O 1.0 g MnCl2 x 4 H2O 1.0 g ZNSO4 x 7 H2O in 100 ml water Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.2 Usage: Maintenance and taxonomy Organisms: All Actinomycetes | |
18687 | ISP 6 | yes | Name: ISP 6 (5318) Composition Peptone 15.0 g/l Proteose peptose 5.0 g/l Ferric ammonium citrate 0.5 g/l Sodium glycerophosphate 1.0 g/l Sodium thiosulfate 0.08 g/l Yeast extract 1.0 g/l Agar 15.0 g/l Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Production of melanoid pigments Organisms: All Actinomycetes | |
18687 | ISP 7 | yes | Name: ISP 7 (5322) Composition Glycerol 15.0 g/l L-Tyrosine 0.5 g/l L-Asparagine 1.0 g/l K2HPO4 0.5 g/l NaCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Trace element solution 5343 1.0 ml/l Agar 20.0 Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.3 Usage: Production of melanoid pigments Organisms: All Actinomycetes |
culture temp
@ref | growth | type | temperature | range |
---|---|---|---|---|
10316 | positive | growth | 28 | mesophilic |
18687 | positive | optimum | 28 | mesophilic |
67770 | positive | growth | 28 | mesophilic |
Physiology and metabolism
spore formation
@ref | spore formation | confidence |
---|---|---|
69481 | yes | 100 |
69480 | yes | 100 |
halophily
- @ref: 18687
- salt: NaCl
- growth: positive
- tested relation: maximum
- concentration: 7.5 %
metabolite utilization
@ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
---|---|---|---|---|
68368 | 5291 | gelatin | + | hydrolysis |
68368 | 27897 | tryptophan | - | energy source |
68368 | 16199 | urea | + | hydrolysis |
68368 | 16947 | citrate | + | assimilation |
68368 | 18257 | ornithine | + | degradation |
68368 | 25094 | lysine | + | degradation |
68368 | 29016 | arginine | + | hydrolysis |
metabolite production
@ref | Chebi-ID | metabolite | production |
---|---|---|---|
68368 | 15688 | acetoin | no |
68368 | 35581 | indole | no |
68368 | 16136 | hydrogen sulfide | no |
metabolite tests
@ref | Chebi-ID | metabolite | voges-proskauer-test | indole test |
---|---|---|---|---|
68368 | 15688 | acetoin | - | |
68368 | 35581 | indole | - |
enzymes
@ref | value | activity | ec |
---|---|---|---|
68368 | gelatinase | + | |
68368 | tryptophan deaminase | - | 4.1.99.1 |
68368 | urease | + | 3.5.1.5 |
68368 | ornithine decarboxylase | + | 4.1.1.17 |
68368 | lysine decarboxylase | + | 4.1.1.18 |
68368 | arginine dihydrolase | + | 3.5.3.6 |
68368 | beta-galactosidase | + | 3.2.1.23 |
68382 | alkaline phosphatase | + | 3.1.3.1 |
68382 | esterase (C 4) | - | |
68382 | esterase lipase (C 8) | + | |
68382 | lipase (C 14) | - | |
68382 | leucine arylamidase | + | 3.4.11.1 |
68382 | valine arylamidase | + | |
68382 | cystine arylamidase | + | 3.4.11.3 |
68382 | trypsin | + | 3.4.21.4 |
68382 | alpha-chymotrypsin | - | 3.4.21.1 |
68382 | acid phosphatase | + | 3.1.3.2 |
68382 | naphthol-AS-BI-phosphohydrolase | + | |
68382 | alpha-galactosidase | + | 3.2.1.22 |
68382 | beta-galactosidase | + | 3.2.1.23 |
68382 | beta-glucuronidase | - | 3.2.1.31 |
68382 | alpha-glucosidase | + | 3.2.1.20 |
68382 | beta-glucosidase | + | 3.2.1.21 |
68382 | N-acetyl-beta-glucosaminidase | + | 3.2.1.52 |
68382 | alpha-mannosidase | + | 3.2.1.24 |
68382 | alpha-fucosidase | - | 3.2.1.51 |
API zym
@ref | Alkaline phosphatase | Esterase | Esterase Lipase | Lipase | Leucine arylamidase | Valine arylamidase | Cystine arylamidase | Trypsin | alpha- Chymotrypsin | Acid phosphatase | Naphthol-AS-BI-phosphohydrolase | alpha- Galactosidase | beta- Galactosidase | beta- Glucuronidase | alpha- Glucosidase | beta- Glucosidase | N-acetyl-beta- glucosaminidase | alpha- Mannosidase | alpha- Fucosidase |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
18687 | + | - | + | - | + | + | + | + | - | + | + | + | + | - | + | + | + | + | - |
API 20E
@ref | ONPG | ADH Arg | LDC Lys | ODC | CIT | H2S | URE | TDA Trp | IND | VP | GEL |
---|---|---|---|---|---|---|---|---|---|---|---|
18687 | + | + | + | + | + | - | + | - | - | - | + |
Isolation, sampling and environmental information
isolation
- @ref: 67770
- sample type: Soil
Safety information
risk assessment
@ref | biosafety level | biosafety level comment |
---|---|---|
10316 | 1 | Risk group (German classification) |
18687 | 1 | German classification |
Sequence information
16S sequences
@ref | description | accession | length | database | NCBI tax ID |
---|---|---|---|---|---|
20218 | Streptomyces asterosporus gene for 16S rRNA, partial sequence, strain: NBRC 15872 | AB184706 | 1476 | ena | 285570 |
20218 | Streptomyces asterosporus strain NRRL B-24328 16S ribosomal RNA gene, partial sequence | AY999902 | 1532 | ena | 285570 |
Genome sequences
@ref | description | accession | assembly level | database | NCBI tax ID |
---|---|---|---|---|---|
66792 | Streptomyces calvus DSM 41452 | GCA_006716135 | complete | ncbi | 67282 |
66792 | Streptomyces asterosporus strain DSM 41452 | 285570.6 | complete | patric | 67282 |
GC content
- @ref: 67770
- GC-content: 72.5
- method: genome sequence analysis
Genome-based predictions
predictions
trait | prediction | confidence | training_data |
---|---|---|---|
spore-forming | yes | 100 | no |
motile | no | 94.761 | no |
flagellated | no | 98.072 | no |
gram-positive | yes | 88.021 | no |
anaerobic | no | 99.015 | no |
aerobic | yes | 94.292 | no |
halophile | no | 90.785 | no |
spore-forming | yes | 95.882 | no |
thermophile | no | 97.206 | yes |
glucose-util | yes | 89.451 | no |
glucose-ferment | no | 89.344 | no |
External links
@ref: 10316
culture collection no.: DSM 41452, INMI 16, JCM 6912, VKM Ac-40, BCRC 16848, CGMCC 4.1605, IFO 15872, NBRC 15872, NRRL B-24328
straininfo link
- @ref: 85251
- straininfo: 50002
literature
topic | Pubmed-ID | title | authors | journal | DOI | year | mesh | topic2 |
---|---|---|---|---|---|---|---|---|
Metabolism | 29148157 | New WS9326A Derivatives and One New Annimycin Derivative with Antimalarial Activity are Produced by Streptomyces asterosporus DSM 41452 and Its Mutant. | Zhang S, Zhu J, Zechel DL, Jessen-Trefzer C, Eastman RT, Paululat T, Bechthold A | Chembiochem | 10.1002/cbic.201700428 | 2017 | Amides/chemistry/*pharmacology, Antimalarials/chemistry/metabolism/*pharmacology, Fatty Acids, Unsaturated/biosynthesis/chemistry/*pharmacology, Lactones/chemistry/*pharmacology, Molecular Conformation, *Mutation, Peptides, Cyclic/*biosynthesis/chemistry/*pharmacology, Streptomyces/chemistry/*genetics/*metabolism | |
Phenotype | 30641108 | Genome mining reveals the origin of a bald phenotype and a cryptic nucleocidin gene cluster in Streptomyces asterosporus DSM 41452. | Zhang S, Klementz D, Zhu J, Makitrynskyy R, Ola Pasternak AR, Gunther S, Zechel DL, Bechthold A | J Biotechnol | 10.1016/j.jbiotec.2018.12.016 | 2019 | Adenosine/*analogs & derivatives/metabolism, Bacterial Proteins/*genetics, DNA Transposable Elements, Genes, Bacterial, Genome, Bacterial, Multigene Family, Phenotype, Promoter Regions, Genetic, RNA, Ribosomal, 16S, Streptomyces/*genetics/metabolism, Trans-Activators/*genetics | Metabolism |
Metabolism | 31310500 | Rational Design of Hybrid Natural Products by Utilizing the Promiscuity of an Amide Synthetase. | Zhu J, Zhang S, Zechel DL, Paululat T, Bechthold A | ACS Chem Biol | 10.1021/acschembio.9b00351 | 2019 | Amides/*metabolism, Bacterial Proteins/genetics/*metabolism, Biological Products/*metabolism, Cyclopentanes/*metabolism, Escherichia coli/genetics, Multigene Family, Mutation, Peptide Synthases/genetics/*metabolism, Protein Engineering, Streptomyces/enzymology, Streptomyces lividans/enzymology, Substrate Specificity | Enzymology |
Reference
@id | authors | catalogue | doi/url | title | journal | pubmed |
---|---|---|---|---|---|---|
10316 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 41452) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-41452 | |||
18687 | Wink, J. | http://www.dsmz.de/microorganisms/wink_pdf/DSM41452.pdf | Compendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig | |||
20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |||
20218 | Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P. | 10.1016/j.syapm.2013.11.002 | StrainInfo introduces electronic passports for microorganisms. | Syst Appl Microbiol. 37: 42-50 2014 | 24321274 | |
66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |||
67770 | Curators of the JCM | https://jcm.brc.riken.jp/en/ | ||||
68368 | Automatically annotated from API 20E | |||||
68382 | Automatically annotated from API zym | |||||
69480 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |||
69481 | Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch | 10.21203/rs.3.rs-2527258/v1 | Predictions based on the model GenomeNet Sporulation v. 1 | |||
85251 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID50002.1 | StrainInfo: A central database for resolving microbial strain identifiers |