Strain identifier

BacDive ID: 16186

Type strain: Yes

Species: Streptomyces megasporus

Strain history: INMI 2869.

NCBI tax ID(s): 44060 (species)

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General

@ref: 10340

BacDive-ID: 16186

DSM-Number: 41476

keywords: genome sequence, 16S sequence, Bacteria, thermophilic

description: Streptomyces megasporus DSM 41476 is a thermophilic bacterium that builds an aerial mycelium.

NCBI tax id

  • NCBI tax id: 44060
  • Matching level: species

strain history

@refhistory
10340<- N.S. Agre, INA <- INMI
67770INMI 2869.

doi: 10.13145/bacdive16186.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Kitasatosporales
  • family: Streptomycetaceae
  • genus: Streptomyces
  • species: Streptomyces megasporus
  • full scientific name: Streptomyces megasporus (ex Krassilnikov et al. 1968) Agre 1986

@ref: 10340

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Streptomycetaceae

genus: Streptomyces

species: Streptomyces megasporus

full scientific name: Streptomyces megasporus (ex Krassilnikov et al. 1968) Agre 1986 emend. Nouioui et al. 2018

type strain: yes

Morphology

colony morphology

@refcolony colorincubation periodmedium used
18696Beige (1001)10-14 daysISP 2
18696Colorless10-14 daysISP 3
18696Colorless10-14 daysISP 4
18696Colorless10-14 daysISP 5
18696Colorless10-14 daysISP 6
18696Beige (1001)10-14 daysISP 7

multicellular morphology

@refforms multicellular complexcomplex namecomplex colormedium name
18696yesAerial MyceliumWhiteISP 2
18696noISP 3
18696noISP 4
18696noISP 5
18696yesAerial MyceliumSparse, whiteISP 6
18696noISP 7

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
10340V-8 JUICE AGAR (DSMZ Medium 310)yeshttps://mediadive.dsmz.de/medium/310Name: V-8 JUICE AGAR (DSMZ Medium 310) Composition: Agar 15.0 g/l CaCO3 3.0 g/l V-8 Juice Distilled water
10340GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)yeshttps://mediadive.dsmz.de/medium/65Name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) Composition: Agar 18.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water
18696ISP 2yesName: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes
18696ISP 7yesName: ISP 7 (5322) Composition Glycerol 15.0 g/l L-Tyrosine 0.5 g/l L-Asparagine 1.0 g/l K2HPO4 0.5 g/l NaCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Trace element solution 5343 1.0 ml/l Agar 20.0 Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.3 Usage: Production of melanoid pigments Organisms: All Actinomycetes
18696ISP 6yesName: ISP 6 (5318) Composition Peptone 15.0 g/l Proteose peptose 5.0 g/l Ferric ammonium citrate 0.5 g/l Sodium glycerophosphate 1.0 g/l Sodium thiosulfate 0.08 g/l Yeast extract 1.0 g/l Agar 15.0 g/l Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Production of melanoid pigments Organisms: All Actinomycetes
18696ISP 5yesName: ISP 5 (5323) Composition L-Asparagine 1.0 g/l Glycerol 10.0 g/l K2HPO4 1.0 g/l Salt solution (see preparation) 1.0 ml/l Agar 20.0 g/l Preparation: Salt solution 1.0 g FeSO4 x 7 H2O 1.0 g MnCl2 x 4 H2O 1.0 g ZNSO4 x 7 H2O in 100 ml water Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.2 Usage: Maintenance and taxonomy Organisms: All Actinomycetes
18696ISP 4yesName: ISP 4; DSM 547 Solution I: Difco soluble starch, 10.0 g. Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml. Solution II: CaCO3 2.0 g K2HPO4 (anhydrous) 1.0 g MgSO4 x 7 H2O 1.0 g NaCl 1.0 g (NH4)2SO4 2.0 g Distilled water 500.0 ml Trace salt solution (see below) 1.0 ml The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min. Trace element solution: FeSO4 x 7 H2O 0.1 g MnCl2 x 4 H2O 0.1 g ZnSO4 x 7 H2O 0.1 g Distilled water 100.0 ml
18696ISP 3yesName: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms:

culture temp

@refgrowthtypetemperaturerange
10340positivegrowth45thermophilic
18696positiveoptimum28mesophilic
67770positivegrowth40thermophilic

Physiology and metabolism

halophily

  • @ref: 18696
  • salt: NaCl
  • growth: positive
  • tested relation: maximum
  • concentration: 2.5 %

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
683685291gelatin+hydrolysis
6836827897tryptophan-energy source
6836816199urea+hydrolysis
6836816947citrate+assimilation
6836818257ornithine+degradation
6836825094lysine+degradation
6836829016arginine+hydrolysis

metabolite production

@refChebi-IDmetaboliteproduction
6836815688acetoinno
6836835581indoleno
6836816136hydrogen sulfideno

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testindole test
6836815688acetoin-
6836835581indole-

enzymes

@refvalueactivityec
68368gelatinase+
68368tryptophan deaminase-4.1.99.1
68368urease+3.5.1.5
68368ornithine decarboxylase+4.1.1.17
68368lysine decarboxylase+4.1.1.18
68368arginine dihydrolase+3.5.3.6
68368beta-galactosidase+3.2.1.23

API 20E

@refONPGADH ArgLDC LysODCCITH2SURETDA TrpINDVPGEL
18696+++++-+---+

Isolation, sampling and environmental information

taxonmaps

  • @ref: 69479
  • File name: preview.99_4252.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_15;96_2143;97_2583;98_3204;99_4252&stattab=map
  • Last taxonomy: Streptomyces megasporus subclade
  • 16S sequence: Z68100
  • Sequence Identity:
  • Total samples: 19
  • soil counts: 13
  • aquatic counts: 4
  • animal counts: 1
  • plant counts: 1

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
103401Risk group (German classification)
186961German classification

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218S.megasporus 16S rRNA geneZ681001490ena44060
20218Streptomyces megasporus gene for 16S rRNA, partial sequence, strain: NBRC 14749AB1846171468ena44060

Genome sequences

  • @ref: 67770
  • description: Streptomyces megasporus NRRL B-16372
  • accession: GCA_000718985
  • assembly level: contig
  • database: ncbi
  • NCBI tax ID: 44060

GC content

  • @ref: 67770
  • GC-content: 72.3
  • method: genome sequence analysis

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
motileno94.811no
flagellatedno98.011no
gram-positiveyes90.84no
anaerobicno98.337no
halophileno84.693no
spore-formingyes93.066no
thermophileno95.629no
glucose-utilyes88.613no
aerobicyes91.337no
glucose-fermentno89.926no

External links

@ref: 10340

culture collection no.: DSM 41476, ATCC 43688, HUT 6610, IFO 14749, INA M-22, INMI 2869, JCM 6926, NBRC 14749, NRRL B-16372, VKM Ac-1776

straininfo link

  • @ref: 85248
  • straininfo: 92985

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny17978221Streptomyces radiopugnans sp. nov., a radiation-resistant actinomycete isolated from radiation-polluted soil in China.Mao J, Tang Q, Zhang Z, Wang W, Wei D, Huang Y, Liu Z, Shi Y, Goodfellow MInt J Syst Evol Microbiol10.1099/ijs.0.65027-02007Bacterial Typing Techniques, China, Cobalt Radioisotopes, DNA, Bacterial/analysis, DNA, Ribosomal/analysis, *Gamma Rays, Genes, rRNA, Molecular Sequence Data, Phenotype, RNA, Ribosomal, 16S/genetics, *Radiation Tolerance, Sequence Analysis, DNA, *Soil Microbiology, *Soil Pollutants, Radioactive, Species Specificity, Streptomyces/*classification/genetics/isolation & purification/physiology/*radiation effectsGenetics
Metabolism22806864A new xylanase from Streptomyces megasporus DSM 41476 with high yield of xylobiose.Shi P, Qiu Z, Bai Y, Yuan T, Huang H, Pan X, Yang P, Zhang W, Yao BWorld J Microbiol Biotechnol10.1007/s11274-011-0863-72011Amino Acid Sequence, Disaccharides/*biosynthesis, Endo-1,4-beta Xylanases/*chemistry/genetics/*metabolism, Hydrogen-Ion Concentration, Molecular Sequence Data, Streptomyces/*enzymology/*metabolism, Substrate SpecificityGenetics
Phylogeny24436069Streptomyces barkulensis sp. nov., isolated from an estuarine lake.Ray L, Mishra SR, Panda AN, Rastogi G, Pattanaik AK, Adhya TK, Suar M, Raina VInt J Syst Evol Microbiol10.1099/ijs.0.056614-02014Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Estuaries, Fatty Acids/chemistry, Geologic Sediments/microbiology, India, Lakes/*microbiology, Molecular Sequence Data, Nucleic Acid Hybridization, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Streptomyces/*classification/genetics/isolation & purification, Vitamin K 2/analogs & derivatives/chemistryGenetics
25919627A xylanase with broad pH and temperature adaptability from Streptomyces megasporus DSM 41476, and its potential application in brewing industry.Qiu Z, Shi P, Luo H, Bai Y, Yuan T, Yang P, Liu S, Yao BEnzyme Microb Technol10.1016/j.enzmictec.2010.02.0032010

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
10340Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 41476)https://www.dsmz.de/collection/catalogue/details/culture/DSM-41476
18696Wink, J.http://www.dsmz.de/microorganisms/wink_pdf/DSM41476.pdfCompendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68368Automatically annotated from API 20E
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
85248Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID92985.1StrainInfo: A central database for resolving microbial strain identifiers