Strain identifier

BacDive ID: 16177

Type strain: Yes

Species: Streptomyces violaceoruber

Strain Designation: 69

Strain history: KCC S-1058 <-- MS 1456 <-- ATCC 19821 <-- N. A. Krassilnikov 69.

NCBI tax ID(s): 68174 (species)

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General

@ref: 10286

BacDive-ID: 16177

DSM-Number: 41422

keywords: genome sequence, 16S sequence, Bacteria, spore-forming, mesophilic, Gram-positive

description: Streptomyces violaceoruber 69 is a spore-forming, mesophilic, Gram-positive bacterium that builds an aerial mycelium and was isolated from soil.

NCBI tax id

  • NCBI tax id: 68174
  • Matching level: species

strain history

@refhistory
10286<- NRRL <- ATCC <- N. A. Krasilnikov, 69
67770KCC S-1058 <-- MS 1456 <-- ATCC 19821 <-- N. A. Krassilnikov 69.

doi: 10.13145/bacdive16177.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Kitasatosporales
  • family: Streptomycetaceae
  • genus: Streptomyces
  • species: Streptomyces violaceoruber
  • full scientific name: Streptomyces violaceoruber (Waksman and Curtis 1916) Pridham 1970 (Approved Lists 1980)

  • synonyms

    @refsynonym
    20215Actinopycnidium caeruleum
    20215Actinomyces tricolor
    20215Actinomyces anthocyanicus
    20215Actinomyces violaceolatus
    20215Streptomyces humiferus
    20215Streptomyces anthocyanicus
    20215Streptomyces tricolor
    20215Streptomyces violaceolatus
    20215Streptomyces roseodiastaticus
    20215Actinomyces violaceusruber
    20215Actinomyces coelescens
    20215Actinomyces roseodiastaticus
    20215Streptomyces coelescens

@ref: 10286

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Streptomycetaceae

genus: Streptomyces

species: Streptomyces anthocyanicus

full scientific name: Streptomyces anthocyanicus (Krassilnikov et al. 1965) Pridham 1970

strain designation: 69

type strain: yes

Morphology

cell morphology

  • @ref: 69480
  • gram stain: positive
  • confidence: 100

colony morphology

@refcolony colorincubation periodmedium used
18684Yellow10-14 daysISP 2
18684Yellow10-14 daysISP 3
18684Beige10-14 daysISP 4
18684Yellow10-14 daysISP 5
18684Beige10-14 daysISP 6
18684Beige10-14 daysISP 7

multicellular morphology

@refforms multicellular complexcomplex namemedium name
18684yesAerial MyceliumISP 2
18684yesAerial MyceliumISP 3
18684yesAerial MyceliumISP 4
18684yesAerial MyceliumISP 5
18684noISP 6
18684yesAerial MyceliumISP 7

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
10286GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)yeshttps://mediadive.dsmz.de/medium/65Name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) Composition: Agar 18.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water
18684ISP 2yesName: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes
18684ISP 3yesName: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms:
18684ISP 4yesName: ISP 4; DSM 547 Solution I: Difco soluble starch, 10.0 g. Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml. Solution II: CaCO3 2.0 g K2HPO4 (anhydrous) 1.0 g MgSO4 x 7 H2O 1.0 g NaCl 1.0 g (NH4)2SO4 2.0 g Distilled water 500.0 ml Trace salt solution (see below) 1.0 ml The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min. Trace element solution: FeSO4 x 7 H2O 0.1 g MnCl2 x 4 H2O 0.1 g ZnSO4 x 7 H2O 0.1 g Distilled water 100.0 ml
18684ISP 5yesName: ISP 5 (5323) Composition L-Asparagine 1.0 g/l Glycerol 10.0 g/l K2HPO4 1.0 g/l Salt solution (see preparation) 1.0 ml/l Agar 20.0 g/l Preparation: Salt solution 1.0 g FeSO4 x 7 H2O 1.0 g MnCl2 x 4 H2O 1.0 g ZNSO4 x 7 H2O in 100 ml water Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.2 Usage: Maintenance and taxonomy Organisms: All Actinomycetes
18684ISP 6yesName: ISP 6 (5318) Composition Peptone 15.0 g/l Proteose peptose 5.0 g/l Ferric ammonium citrate 0.5 g/l Sodium glycerophosphate 1.0 g/l Sodium thiosulfate 0.08 g/l Yeast extract 1.0 g/l Agar 15.0 g/l Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Production of melanoid pigments Organisms: All Actinomycetes
18684ISP 7yesName: ISP 7 (5322) Composition Glycerol 15.0 g/l L-Tyrosine 0.5 g/l L-Asparagine 1.0 g/l K2HPO4 0.5 g/l NaCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Trace element solution 5343 1.0 ml/l Agar 20.0 Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.3 Usage: Production of melanoid pigments Organisms: All Actinomycetes

culture temp

@refgrowthtypetemperaturerange
10286positivegrowth28mesophilic
18684positiveoptimum28mesophilic
67770positivegrowth28mesophilic

Physiology and metabolism

spore formation

@refspore formationconfidence
69481yes100
69480yes100

halophily

  • @ref: 18684
  • salt: NaCl
  • growth: positive
  • tested relation: growth
  • concentration: 0 %

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
1868417234glucose+
1868422599arabinose+
1868417992sucrose-
1868418222xylose-
1868417268myo-inositol+
1868429864mannitol+
1868428757fructose+
1868426546rhamnose+
1868416634raffinose-
1868462968cellulose-
683685291gelatin+hydrolysis
6836827897tryptophan-energy source
6836816199urea+hydrolysis
6836816947citrate+assimilation
6836818257ornithine-degradation
6836825094lysine+degradation
6836829016arginine+hydrolysis

metabolite production

@refChebi-IDmetaboliteproduction
6836815688acetoinyes
6836835581indoleno
6836816136hydrogen sulfideno

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testindole test
6836815688acetoin+
6836835581indole-

enzymes

@refvalueactivityec
68368gelatinase+
68368tryptophan deaminase-4.1.99.1
68368urease+3.5.1.5
68368ornithine decarboxylase-4.1.1.17
68368lysine decarboxylase+4.1.1.18
68368arginine dihydrolase+3.5.3.6
68368beta-galactosidase+3.2.1.23

API 20E

@refONPGADH ArgLDC LysODCCITH2SURETDA TrpINDVPGEL
18684+++-+-+--++

Isolation, sampling and environmental information

isolation

@refsample typecountryorigin.country
10286soil
67770SoilRussiaRUS

isolation source categories

  • Cat1: #Environmental
  • Cat2: #Terrestrial
  • Cat3: #Soil

taxonmaps

  • @ref: 69479
  • File name: preview.99_357.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_15;96_91;97_97;98_106;99_357&stattab=map
  • Last taxonomy: Streptomyces
  • 16S sequence: AB184631
  • Sequence Identity:
  • Total samples: 5402
  • soil counts: 2201
  • aquatic counts: 991
  • animal counts: 1970
  • plant counts: 240

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
102861Risk group (German classification)
186841German classification

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Streptomyces anthocyanicus gene for 16S ribosomal RNA, partial sequence, strain: JCM 5058D44427121ena68174
20218Streptomyces anthocyanicus strain KCTC 9755 16S ribosomal RNA gene, partial sequenceAY9998331421ena68174
20218Streptomyces anthocyanicus gene for 16S rRNA, partial sequence, strain: NBRC 14892AB1846311478ena68174

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Streptomyces anthocyanicus strain JCM 505868174.3wgspatric68174
67770Streptomyces anthocyanicus JCM 5058GCA_014651155scaffoldncbi68174

GC content

  • @ref: 67770
  • GC-content: 72.3
  • method: genome sequence analysis

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingyes100no
gram-positiveyes88.874no
anaerobicno99.255no
halophileno92.122yes
spore-formingyes93.997no
glucose-utilyes89.554no
thermophileno98.962no
flagellatedno97.871no
motileno93.933no
aerobicyes94.119no
glucose-fermentno89.901no

External links

@ref: 10286

culture collection no.: DSM 41422, ATCC 19821, INMI 69, NRRL B-12341, JCM 5058, CGMCC 4.1683, IFO 14892, KCTC 9755, NBRC 14892, NRRL B-24292

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
10286Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 41422)https://www.dsmz.de/collection/catalogue/details/culture/DSM-41422
18684Wink, J.http://www.dsmz.de/microorganisms/wink_pdf/DSM41422.pdfCompendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68368Automatically annotated from API 20E
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1