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Strain identifier

BacDive ID: 16158

Type strain: Yes

Species: Streptomyces eurocidicus

Strain Designation: 549-A1

Strain history: KCC S-0029 <-- Y. Okami 549-A1 (NIHJ 267).

NCBI tax ID(s): 66423 (species)

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@ref: 9679

BacDive-ID: 16158

DSM-Number: 40604

keywords: genome sequence, 16S sequence, Bacteria, spore-forming, mesophilic, antibiotic compound production

description: Streptomyces eurocidicus 549-A1 is a spore-forming, mesophilic bacterium that builds an aerial mycelium and produces antibiotic compounds.

NCBI tax id

  • NCBI tax id: 66423
  • Matching level: species

strain history

doi: 10.13145/bacdive16158.20221219.7.1

Name and taxonomic classification


  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Streptomycetales
  • family: Streptomycetaceae
  • genus: Streptomyces
  • species: Streptomyces eurocidicus
  • full scientific name: Streptomyces eurocidicus (Okami et al. 1954) Witt and Stackebrandt 1991
  • synonyms

    20215Streptoverticillium eurocidicum
    20215Streptoverticillium albireticuli
    20215Streptomyces eurocidicus
    20215Streptomyces albireticuli

@ref: 9679

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Streptomycetaceae

genus: Streptomyces

species: Streptomyces eurocidicus

full scientific name: Streptomyces eurocidicus (Okami et al. 1954) Witt and Stackebrandt 1991

strain designation: 549-A1

type strain: yes


cell morphology

  • @ref: 69480
  • gram stain: positive
  • confidence: 95.818

colony morphology

@refcolony colorincubation periodmedium used
18662Nut brown (8011)10-14 daysISP 2
18662Ivory (1014)10-14 daysISP 3
18662Sand yellow (1002)10-14 daysISP 4
18662Ivory (1014)10-14 daysISP 5
18662Olive brown (8008)10-14 daysISP 6
18662Ochre yellow (1024)10-14 daysISP 7

multicellular morphology

@refforms multicellular complexcomplex namecomplex colormedium name
18662yesAerial MyceliumPebble grey (7032)ISP 2
18662yesAerial MyceliumPebble grey (7032)ISP 3
18662yesAerial MyceliumIvory (1014)ISP 4
18662yesAerial MyceliumBrown beige (1011)ISP 5
18662yesAerial MyceliumBeige grey (7006)ISP 6
18662yesAerial MyceliumBeige grey (7006)ISP 7


  • @ref: 9679
  • multimedia content: https://www.dsmz.de/microorganisms/photos/DSM_40604.jpg
  • caption: Medium 65 28°C
  • intellectual property rights: © Leibniz-Institut DSMZ

Culture and growth conditions

culture medium

9679ROLLED OATS MINERAL MEDIUM (DSMZ Medium 84)yeshttps://mediadive.dsmz.de/medium/84Name: ROLLED OATS MINERAL MEDIUM (DSMZ Medium 84) Composition: Agar 20.0 g/l Rolled oats 20.0 g/l ZnSO4 x 7 H2O 0.001 g/l MnCl2 x 4 H2O 0.001 g/l FeSO4 x 7 H2O 0.001 g/l Distilled water
9679STARCH - MINERAL SALT - AGAR (STMS) (DSMZ Medium 252)yeshttps://mediadive.dsmz.de/medium/252Name: STARCH - MINERAL SALT - AGAR (STMS) (DSMZ Medium 252) Composition: Agar 14.985 g/l Starch 9.99001 g/l (NH4)2SO4 1.998 g/l CaCO3 1.998 g/l K2HPO4 0.999001 g/l MgSO4 x 7 H2O 0.999001 g/l NaCl 0.999001 g/l FeSO4 x 7 H2O 0.000999001 g/l MnCl2 x 4 H2O 0.000999001 g/l ZnSO4 x 7 H2O 0.000999001 g/l Distilled water
9679GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)yeshttps://mediadive.dsmz.de/medium/65Name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) Composition: Agar 18.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water
18662ISP 2yesName: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes
18662ISP 3yesName: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms:
18662ISP 4yesName: ISP 4; DSM 547 Solution I: Difco soluble starch, 10.0 g. Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml. Solution II: CaCO3 2.0 g K2HPO4 (anhydrous) 1.0 g MgSO4 x 7 H2O 1.0 g NaCl 1.0 g (NH4)2SO4 2.0 g Distilled water 500.0 ml Trace salt solution (see below) 1.0 ml The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min. Trace element solution: FeSO4 x 7 H2O 0.1 g MnCl2 x 4 H2O 0.1 g ZnSO4 x 7 H2O 0.1 g Distilled water 100.0 ml
18662ISP 5yesName: ISP 5 (5323) Composition L-Asparagine 1.0 g/l Glycerol 10.0 g/l K2HPO4 1.0 g/l Salt solution (see preparation) 1.0 ml/l Agar 20.0 g/l Preparation: Salt solution 1.0 g FeSO4 x 7 H2O 1.0 g MnCl2 x 4 H2O 1.0 g ZNSO4 x 7 H2O in 100 ml water Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.2 Usage: Maintenance and taxonomy Organisms: All Actinomycetes
18662ISP 6yesName: ISP 6 (5318) Composition Peptone 15.0 g/l Proteose peptose 5.0 g/l Ferric ammonium citrate 0.5 g/l Sodium glycerophosphate 1.0 g/l Sodium thiosulfate 0.08 g/l Yeast extract 1.0 g/l Agar 15.0 g/l Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Production of melanoid pigments Organisms: All Actinomycetes
18662ISP 7yesName: ISP 7 (5322) Composition Glycerol 15.0 g/l L-Tyrosine 0.5 g/l L-Asparagine 1.0 g/l K2HPO4 0.5 g/l NaCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Trace element solution 5343 1.0 ml/l Agar 20.0 Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.3 Usage: Production of melanoid pigments Organisms: All Actinomycetes
36696MEDIUM 57 - for Streptomyces, Nocardioides, Lentzea albidocapillata and Streptoverticillium reticulumyesDistilled water make up to (1000.000 ml);Agar (15.000 g);Glucose (4.000g);Yeast extract (4.000 g);Malt extract (10.000 g);Calcium carbonate (2.000 g)

culture temp


Physiology and metabolism

spore formation

@refspore descriptionspore formationconfidence
18662Formation of spore chains (verticillati)(Ve), spore surface smoothyes

compound production

9679tertiomycine A
9679tertiomycine B
20216Azomycin (2-nitroimidazole)
20216Tertiomycin A
20216Tertiomycin B
18662Macrolide antibiotics like Antibiotic YS 822A
18662Eurocidin D
18662Aminoglycoside antibiotics like Antibiotic SS 56A


  • @ref: 69480
  • halophily level: non-halophilic
  • confidence: 97.32

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6836827897tryptophan-energy source

metabolite production

67770tertiomycin Ayes
67770tertiomycin Byes
6836816136hydrogen sulfideno

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testindole test


68382acid phosphatase+
68382cystine arylamidase+
68382valine arylamidase+
68382leucine arylamidase+
68382lipase (C 14)-
68382esterase lipase (C 8)+
68382esterase (C 4)+
68382alkaline phosphatase+
68368tryptophan deaminase-
68368ornithine decarboxylase+
68368lysine decarboxylase+
68368arginine dihydrolase+

API zym

@refAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase



Isolation, sampling and environmental information


  • @ref: 67770
  • sample type: Soil

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
186621German classification
96791Risk group (German classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Streptomyces eurocidicus gene for 16S ribosomal RNA, partial sequence, strain: JCM 4029D43989120ena66423
20218Streptomyces eurocidicus gene for 16S rRNA, partial sequence, strain: NBRC 13491AB1844411473ena66423
20218Streptomyces eurocidicus strain NRRL B-1676 16S ribosomal RNA gene, partial sequenceAY9997901492ena66423

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Streptomyces eurocidicus CECT 3259GCA_014203505scaffoldncbi66423
66792Streptomyces eurocidicus strain ATCC 2742866423.13wgspatric66423
66792Streptomyces eurocidicus strain CECT 325966423.15wgspatric66423
66792Streptomyces eurocidicus CECT 32592863257063draftimg66423
66792Streptomyces eurocidicus ATCC 274282862643501draftimg66423
67770Streptomyces eurocidicus ATCC 27428GCA_002891295contigncbi66423

External links

@ref: 9679

culture collection no.: DSM 40604, ATCC 27428, CBS 792.72, IFO 13491, ISP 5604, JCM 4029, NBRC 13491, NIHJ 267, NRRL B-1676, RIA 1452, RIA 733, BCRC 12424, CECT 3259, CGMCC 4.1086, CIP 108155, IMET 43412, JCM 4749, KCTC 9884, MTCC 2531, NCB 264, VKM Ac-903

straininfo link



Phylogeny2358398Studies on inhibitors of rat mast cell degranulation produced by microorganisms. I. Screening of microorganisms, and isolation and physico-chemical properties of eurocidins C, D and E.Nakagomi K, Takeuchi M, Tanaka H, Tomizuka N, Nakajima TJ Antibiot (Tokyo)10.7164/antibiotics.43.4621990Animals, Anti-Inflammatory Agents, Non-Steroidal/analysis/isolation & purification/*pharmacology, Cell Degranulation/*drug effects, Cells, Cultured, Chromatography, High Pressure Liquid, Chromatography, Ion Exchange, Mast Cells/*drug effects/physiology, Molecular Weight, Polyenes/analysis/isolation & purification/pharmacology, Rats, Rats, Inbred Strains, Spectrophotometry, Infrared, Streptomycetaceae/metabolismEnzymology
Phylogeny27169711Streptomyces zhihengii sp. nov., isolated from rhizospheric soil of Psammosilene tunicoides.Huang MJ, Fei JJ, Salam N, Kim CJ, Hozzein WN, Xiao M, Huang HQ, Li WJArch Microbiol10.1007/s00203-016-1233-52016Bacterial Typing Techniques, Base Composition/genetics, Caryophyllaceae/*microbiology, China, DNA, Bacterial/genetics, Diaminopimelic Acid/analysis, Fatty Acids/analysis, Nucleic Acid Hybridization, Peptidoglycan/chemistry, Phylogeny, RNA, Ribosomal, 16S/genetics, *Rhizosphere, Sequence Analysis, DNA, Soil Microbiology, Streptomyces/*classification/genetics/*isolation & purificationTranscriptome
Phylogeny34382928Streptomyces genisteinicus sp. nov., a novel genistein-producing actinomycete isolated from a Chinese medicinal plant and proposal of Streptomyces michiganensis Corbaz et al. 1957 as a later heterotypic synonym of Streptomyces xanthochromogenes Arishima et al. 1956.Hu S, Li K, Wang Y, Guo Y, Zhou M, Tang X, Gao JInt J Syst Evol Microbiol10.1099/ijsem.0.0049542021Bacterial Typing Techniques, Base Composition, China, DNA, Bacterial/genetics, Fatty Acids/chemistry, Genistein/*metabolism, Nucleic Acid Hybridization, *Phylogeny, Plant Leaves/microbiology, Plants, Medicinal/microbiology, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, *Streptomyces/classification/isolation & purification, Xanthium/*microbiologyTranscriptome


9679Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 40604)https://www.dsmz.de/collection/catalogue/details/culture/DSM-40604
18662Wink, J.http://www.dsmz.de/microorganisms/wink_pdf/DSM40604.pdfCompendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20216Curators of the HKIhttp://www.leibniz-hki.de/de/Collection Description Leibniz-Institut für Naturstoff-Forschung und Infektionsbiologie e. V. Hans-Knöll-Institut (HKI)
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
36696Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/5746
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68368Automatically annotated from API 20E
68382Automatically annotated from API zym
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)