Strain identifier

BacDive ID: 16146

Type strain: Yes

Species: Streptomyces ardus

Strain history: CIP <- 2003, JCM <- 1983, KCC <- IFO <- SAJ <- ISP <- NRRL <- UC: strain UC 2500

NCBI tax ID(s): 67273 (species)

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General

@ref: 9616

BacDive-ID: 16146

DSM-Number: 40527

keywords: 16S sequence, Bacteria, obligate aerobe, mesophilic, Gram-positive, rod-shaped, antibiotic compound production

description: Streptomyces ardus DSM 40527 is an obligate aerobe, mesophilic, Gram-positive bacterium that builds an aerial mycelium and produces antibiotic compounds.

NCBI tax id

  • NCBI tax id: 67273
  • Matching level: species

strain history

@refhistory
9616<- E.B. Shirling, ISP <- NRRL <- A. Dietz, Upjohn Co., UC 2500
67770KCC S-0543 <-- IPV 2020 <-- Upjohn Co., USA; UC 2500.
116482CIP <- 2003, JCM <- 1983, KCC <- IFO <- SAJ <- ISP <- NRRL <- UC: strain UC 2500

doi: 10.13145/bacdive16146.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Kitasatosporales
  • family: Streptomycetaceae
  • genus: Streptomyces
  • species: Streptomyces ardus
  • full scientific name: Streptomyces ardus (De Boer et al. 1961) Witt and Stackebrandt 1991

  • synonyms

    @refsynonym
    20215Streptoverticillium ardum
    20215Streptomyces ardus

@ref: 9616

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Streptomycetaceae

genus: Streptomyces

species: Streptomyces ardus

full scientific name: Streptomyces ardus (De Boer et al. 1961) Witt and Stackebrandt 1991

type strain: yes

Morphology

cell morphology

  • @ref: 116482
  • gram stain: positive
  • cell shape: rod-shaped
  • motility: no

colony morphology

@refcolony colorincubation periodmedium used
19471Beige10-14 daysISP 2
19471Beige10-14 daysISP 3
19471Beige10-14 daysISP 4
19471Beige10-14 daysISP 5
19471Beige10-14 daysISP 6
19471Beige10-14 daysISP 7
116482

multicellular morphology

@refforms multicellular complexmedium namecomplex name
19471noISP 2
19471noISP 3
19471noISP 4
19471noISP 5
19471yesISP 6Aerial Mycelium
19471noISP 7

multimedia

  • @ref: 9616
  • multimedia content: https://www.dsmz.de/microorganisms/photos/DSM_40527.jpg
  • caption: Medium 65 28°C
  • intellectual property rights: © Leibniz-Institut DSMZ

Culture and growth conditions

culture medium

@refnamegrowthcompositionlink
19471ISP 2yesName: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes
19471ISP 3yesName: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms:
19471ISP 4yesName: ISP 4; DSM 547 Solution I: Difco soluble starch, 10.0 g. Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml. Solution II: CaCO3 2.0 g K2HPO4 (anhydrous) 1.0 g MgSO4 x 7 H2O 1.0 g NaCl 1.0 g (NH4)2SO4 2.0 g Distilled water 500.0 ml Trace salt solution (see below) 1.0 ml The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min. Trace element solution: FeSO4 x 7 H2O 0.1 g MnCl2 x 4 H2O 0.1 g ZnSO4 x 7 H2O 0.1 g Distilled water 100.0 ml
19471ISP 5yesName: ISP 5 (5323) Composition L-Asparagine 1.0 g/l Glycerol 10.0 g/l K2HPO4 1.0 g/l Salt solution (see preparation) 1.0 ml/l Agar 20.0 g/l Preparation: Salt solution 1.0 g FeSO4 x 7 H2O 1.0 g MnCl2 x 4 H2O 1.0 g ZNSO4 x 7 H2O in 100 ml water Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.2 Usage: Maintenance and taxonomy Organisms: All Actinomycetes
19471ISP 6yesName: ISP 6 (5318) Composition Peptone 15.0 g/l Proteose peptose 5.0 g/l Ferric ammonium citrate 0.5 g/l Sodium glycerophosphate 1.0 g/l Sodium thiosulfate 0.08 g/l Yeast extract 1.0 g/l Agar 15.0 g/l Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Production of melanoid pigments Organisms: All Actinomycetes
19471ISP 7yesName: ISP 7 (5322) Composition Glycerol 15.0 g/l L-Tyrosine 0.5 g/l L-Asparagine 1.0 g/l K2HPO4 0.5 g/l NaCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Trace element solution 5343 1.0 ml/l Agar 20.0 Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.3 Usage: Production of melanoid pigments Organisms: All Actinomycetes
33898MEDIUM 57 - for Streptomyces, Nocardioides, Lentzea albidocapillata and Streptoverticillium reticulumyesDistilled water make up to (1000.000 ml);Agar (15.000 g);Glucose (4.000g);Yeast extract (4.000 g);Malt extract (10.000 g);Calcium carbonate (2.000 g)
116482CIP Medium 57yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=57

culture temp

@refgrowthtypetemperaturerange
19471positiveoptimum28mesophilic
33898positivegrowth30mesophilic
67770positivegrowth28mesophilic
116482positivegrowth25-37mesophilic
116482nogrowth10psychrophilic
116482nogrowth41thermophilic
116482nogrowth45thermophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 116482
  • oxygen tolerance: obligate aerobe

compound production

@refcompound
9616porfiromycin
19471Porfiromycin (Methylmitomycin C)

halophily

@refsaltgrowthtested relationconcentration
116482NaClpositivegrowth0-4 %
116482NaClnogrowth6 %
116482NaClnogrowth8 %
116482NaClnogrowth10 %

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
1947162968cellulose-
1947116634raffinose-
1947126546rhamnose-
1947128757fructose+
1947129864mannitol-
1947117268myo-inositol+
1947118222xylose-
1947117992sucrose-
1947122599arabinose-
1947117234glucose+
683685291gelatin+hydrolysis
6836827897tryptophan-energy source
6836816199urea+hydrolysis
6836816947citrate+assimilation
6836818257ornithine+degradation
6836825094lysine+degradation
6836829016arginine+hydrolysis
11648216947citrate-carbon source
1164824853esculin-hydrolysis
116482606565hippurate+hydrolysis
11648217632nitrate-reduction
11648216301nitrite-reduction
11648217632nitrate-respiration

metabolite production

@refmetaboliteproductionChebi-ID
67770porfiromycinyes
68368acetoinno15688
68368indoleno35581
68368hydrogen sulfideyes16136
116482indoleno35581

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testindole testmethylred-test
6836815688acetoin-
6836835581indole-
11648215688acetoin-
11648217234glucose-

enzymes

@refvalueactivityec
68368gelatinase+
68368tryptophan deaminase-4.1.99.1
68368urease+3.5.1.5
68368ornithine decarboxylase+4.1.1.17
68368lysine decarboxylase+4.1.1.18
68368arginine dihydrolase+3.5.3.6
68368beta-galactosidase+3.2.1.23
116482oxidase-
116482beta-galactosidase-3.2.1.23
116482alcohol dehydrogenase-1.1.1.1
116482gelatinase-
116482amylase-
116482DNase-
116482caseinase-3.4.21.50
116482catalase+1.11.1.6
116482tween esterase-
116482gamma-glutamyltransferase+2.3.2.2
116482lecithinase+
116482lipase+
116482lysine decarboxylase-4.1.1.18
116482ornithine decarboxylase-4.1.1.17
116482phenylalanine ammonia-lyase-4.3.1.24
116482tryptophan deaminase-
116482urease+3.5.1.5
68382alkaline phosphatase+3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)+
68382lipase (C 14)-
68382leucine arylamidase+3.4.11.1
68382valine arylamidase+
68382cystine arylamidase+3.4.11.3
68382trypsin+3.4.21.4
68382alpha-chymotrypsin+3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase-3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase+3.2.1.20
68382alpha-mannosidase+3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- FucosidaseControl
19471+++-+++++++---++++-
116482+++-+++++++---+--+--

API 20E

@refONPGADH ArgLDC LysODCCITH2SURETDA TrpINDVPGEL
19471+++++++---+

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
116482+++++----++--------+-----------++--------------+--+--+-----++-----------++----+-----+-+--+++-++-++-

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinent
9616soil
67770SoilMOUSAUSANorth America
116482Environment, SoilMissouriUnited States of AmericaUSANorth America

isolation source categories

  • Cat1: #Environmental
  • Cat2: #Terrestrial
  • Cat3: #Soil

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
96161Risk group (German classification)
194711Risk group (German classification)
1164821Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Streptomyces ardus strain DSM 40527 16S ribosomal RNA gene, partial sequenceAY9998431420ena67273
20218Streptomyces ardus gene for 16S ribosomal RNA, partial sequence, strain: JCM 4543D44223120ena67273
20218Streptomyces ardus gene for 16S rRNA, partial sequence, strain: NBRC 13430AB1848641457ena67273

External links

@ref: 9616

culture collection no.: DSM 40527, ATCC 27417, CBS 731.72, IFO 13430, ISP 5527, JCM 4543, JCM 4722, NBRC 13430, NRRL 2817, RIA 1391, UC 2500, BCRC 12319, CECT 3254, CGMCC 4.1670, CIP 108147, KCTC 9887, NBIMCC 1592, NCB 99, VKM Ac-930, CCRC 12319, KCC S-0722, AS 4.167

straininfo link

  • @ref: 85211
  • straininfo: 38996

literature

  • topic: Phylogeny
  • Pubmed-ID: 17342048
  • title: Production and biological activity of laidlomycin, anti-MRSA/VRE antibiotic from Streptomyces sp. CS684.
  • authors: Yoo JC, Kim JH, Ha JW, Park NS, Sohng JK, Lee JW, Park SC, Kim MS, Seong CN
  • journal: J Microbiol
  • DOI: 2499
  • year: 2007
  • mesh: Anti-Bacterial Agents/*biosynthesis/pharmacology, Drug Resistance, Bacterial, Korea, Methicillin Resistance, Monensin/*analogs & derivatives/biosynthesis/pharmacology, Phylogeny, Soil Microbiology, Staphylococcus aureus/*drug effects, Streptomyces/classification/*metabolism, Vancomycin Resistance
  • topic2: Biotechnology

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
9616Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 40527)https://www.dsmz.de/collection/catalogue/details/culture/DSM-40527
19471Wink, J.http://www.dsmz.de/microorganisms/wink_pdf/DSM40527.pdfCompendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
33898Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/5737
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68368Automatically annotated from API 20E
68382Automatically annotated from API zym
85211Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID38996.1StrainInfo: A central database for resolving microbial strain identifiers
116482Curators of the CIPCollection of Institut Pasteur (CIP 108147)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20108147