Strain identifier

BacDive ID: 16145

Type strain: Yes

Species: Streptomyces luteosporeus

Strain Designation: BA 3972, BA-3972

Strain history: CIP <- 2003, JCM <- 1983, KCC <- 1993, IPV <- NRRL, Streptomyces albus <- Chas. Pfizer & Co.: strain BA-3972

NCBI tax ID(s): 173856 (species)

« back to strain detail view
For citation purpose refer to the digital object identifier (doi) of the current version.
Archive
version 8.1 (current version):
version 8:
version 7.1:
version 7:
version 6:
version 5:
version 4.1:
version 4:
version 3:
version 2.1:
version 2:
version 1:
version 8.1 (current version)

General

@ref: 9836

BacDive-ID: 16145

DSM-Number: 40833

keywords: 16S sequence, Bacteria, obligate aerobe, spore-forming, mesophilic, Gram-positive, rod-shaped, antibiotic compound production

description: Streptomyces luteosporeus BA 3972 is an obligate aerobe, spore-forming, mesophilic bacterium that builds an aerial mycelium and produces antibiotic compounds.

NCBI tax id

  • NCBI tax id: 173856
  • Matching level: species

strain history

@refhistory
9836<- NRRL (Streptomyces albus) <- Chas. Pfizer & Co., BA 3972
67770KCC S-0542 <-- IPV 1993 <-- NRRL 2401 <-- Chas. Pfizer & Co.; BA-3972.
120696CIP <- 2003, JCM <- 1983, KCC <- 1993, IPV <- NRRL, Streptomyces albus <- Chas. Pfizer & Co.: strain BA-3972

doi: 10.13145/bacdive16145.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Kitasatosporales
  • family: Streptomycetaceae
  • genus: Streptomyces
  • species: Streptomyces luteosporeus
  • full scientific name: Streptomyces luteosporeus Witt and Stackebrandt 1991

  • synonyms

    • @ref: 20215
    • synonym: Streptoverticillium album

@ref: 9836

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Streptomycetaceae

genus: Streptomyces

species: Streptomyces luteosporeus

full scientific name: Streptomyces luteosporeus Witt and Stackebrandt 1991

strain designation: BA 3972, BA-3972

type strain: yes

Morphology

cell morphology

  • @ref: 120696
  • gram stain: positive
  • cell shape: rod-shaped
  • motility: no

colony morphology

@refcolony colorincubation periodmedium usedhemolysis ability
19490Yellow10-14 daysISP 2
1949010-14 daysISP 3
1949010-14 daysISP 4
1949010-14 daysISP 5
1949010-14 daysISP 6
1949010-14 daysISP 7
1206961

multicellular morphology

@refforms multicellular complexcomplex namecomplex colormedium name
19490yesAerial MyceliumWhiteISP 2
19490yesAerial MyceliumWhiteISP 3
19490yesAerial MyceliumWhiteISP 4
19490yesAerial MyceliumWhiteISP 5
19490noISP 6
19490yesAerial MyceliumWhiteISP 7

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
9836GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)yeshttps://mediadive.dsmz.de/medium/65Name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) Composition: Agar 18.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water
19490ISP 2yesName: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes
19490ISP 3yesName: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms:
19490ISP 4yesName: ISP 4; DSM 547 Solution I: Difco soluble starch, 10.0 g. Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml. Solution II: CaCO3 2.0 g K2HPO4 (anhydrous) 1.0 g MgSO4 x 7 H2O 1.0 g NaCl 1.0 g (NH4)2SO4 2.0 g Distilled water 500.0 ml Trace salt solution (see below) 1.0 ml The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min. Trace element solution: FeSO4 x 7 H2O 0.1 g MnCl2 x 4 H2O 0.1 g ZnSO4 x 7 H2O 0.1 g Distilled water 100.0 ml
19490ISP 5yesName: ISP 5 (5323) Composition L-Asparagine 1.0 g/l Glycerol 10.0 g/l K2HPO4 1.0 g/l Salt solution (see preparation) 1.0 ml/l Agar 20.0 g/l Preparation: Salt solution 1.0 g FeSO4 x 7 H2O 1.0 g MnCl2 x 4 H2O 1.0 g ZNSO4 x 7 H2O in 100 ml water Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.2 Usage: Maintenance and taxonomy Organisms: All Actinomycetes
19490ISP 6yesName: ISP 6 (5318) Composition Peptone 15.0 g/l Proteose peptose 5.0 g/l Ferric ammonium citrate 0.5 g/l Sodium glycerophosphate 1.0 g/l Sodium thiosulfate 0.08 g/l Yeast extract 1.0 g/l Agar 15.0 g/l Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Production of melanoid pigments Organisms: All Actinomycetes
19490ISP 7yesName: ISP 7 (5322) Composition Glycerol 15.0 g/l L-Tyrosine 0.5 g/l L-Asparagine 1.0 g/l K2HPO4 0.5 g/l NaCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Trace element solution 5343 1.0 ml/l Agar 20.0 Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.3 Usage: Production of melanoid pigments Organisms: All Actinomycetes
33205MEDIUM 57 - for Streptomyces, Nocardioides, Lentzea albidocapillata and Streptoverticillium reticulumyesDistilled water make up to (1000.000 ml);Agar (15.000 g);Glucose (4.000g);Yeast extract (4.000 g);Malt extract (10.000 g);Calcium carbonate (2.000 g)
9836STARCH - MINERAL SALT - AGAR (STMS) (DSMZ Medium 252)yeshttps://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium252.pdf
120696CIP Medium 57yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=57

culture temp

@refgrowthtypetemperaturerange
9836positivegrowth28mesophilic
19490positiveoptimum37mesophilic
33205positivegrowth30mesophilic
67770positivegrowth37mesophilic
120696positivegrowth25-45
120696nogrowth10psychrophilic

Physiology and metabolism

tolerance

  • @ref: 19490
  • compound: Lysozyme
  • percentage: 1

oxygen tolerance

  • @ref: 120696
  • oxygen tolerance: obligate aerobe

spore formation

  • @ref: 19490
  • spore description: Formation of spore chains (verticillati)(Ve), spore surface smooth
  • type of spore: spore
  • spore formation: yes

compound production

@refcompound
9836acetopyrrothine
9836thiolutin
9836antibiotic PA-155 A
9836indolmycin

halophily

@refsaltgrowthtested relationconcentration
19490NaClpositivemaximum5 %
120696NaClpositivegrowth0-2 %
120696NaClnogrowth4 %
120696NaClnogrowth6 %
120696NaClnogrowth8 %
120696NaClnogrowth10 %

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
1949062968cellulose-
1949016634raffinose-
1949026546rhamnose-
1949028757fructose-
1949029864mannitol-
1949017268myo-inositol+
1949018222xylose-
1949017992sucrose-
1949022599arabinose-
1949017234glucose+
683685291gelatin+hydrolysis
6836827897tryptophan-energy source
6836816199urea+hydrolysis
6836816947citrate+assimilation
6836818257ornithine+degradation
6836825094lysine+degradation
6836829016arginine+hydrolysis
12069616947citrate-carbon source
1206964853esculin-hydrolysis
120696606565hippurate+hydrolysis
12069617632nitrate-reduction
12069616301nitrite-reduction
12069617632nitrate-respiration

antibiotic resistance

  • @ref: 120696
  • metabolite: 0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)
  • is antibiotic: yes
  • is sensitive: no
  • is resistant: yes

metabolite production

@refChebi-IDmetaboliteproduction
6777079394indolmycinyes
6836815688acetoinyes
6836835581indoleno
6836816136hydrogen sulfideno
12069635581indoleno

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testindole testmethylred-test
6836815688acetoin+
6836835581indole-
12069615688acetoin-
12069617234glucose-

enzymes

@refvalueactivityec
68368gelatinase+
68368tryptophan deaminase-4.1.99.1
68368urease+3.5.1.5
68368ornithine decarboxylase+4.1.1.17
68368lysine decarboxylase+4.1.1.18
68368arginine dihydrolase+3.5.3.6
68368beta-galactosidase-3.2.1.23
120696oxidase-
120696beta-galactosidase+3.2.1.23
120696alcohol dehydrogenase-1.1.1.1
120696gelatinase+/-
120696amylase-
120696DNase-
120696caseinase-3.4.21.50
120696catalase+1.11.1.6
120696tween esterase-
120696gamma-glutamyltransferase+2.3.2.2
120696lecithinase-
120696lipase-
120696lysine decarboxylase-4.1.1.18
120696ornithine decarboxylase-4.1.1.17
120696phenylalanine ammonia-lyase-4.3.1.24
120696tryptophan deaminase-
120696urease+3.5.1.5
68382alkaline phosphatase+3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)+
68382lipase (C 14)-
68382leucine arylamidase+3.4.11.1
68382valine arylamidase+
68382cystine arylamidase+3.4.11.3
68382trypsin-3.4.21.4
68382alpha-chymotrypsin+3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase-3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase+3.2.1.20
68382beta-glucosidase-3.2.1.21
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-mannosidase+3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
120696-+++-+++-+++---+--+-

API 20E

@refONPGADH ArgLDC LysODCCITH2SURETDA TrpINDVPGEL
19490-++++-+--++

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
120696+--+-----++--------------------++----------------+---------++------------+----+++--------+++-++----

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
98361Risk group (German classification)
194901Risk group (German classification)
1206961Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Streptomyces luteosporeus gene for 16S rRNA, partial sequence, strain: NBRC 14657AB1846071476ena173856
20218Streptomyces luteosporeus strain NRRL 2401T 16S ribosomal RNA gene, partial sequenceDQ4425251495ena173856

External links

@ref: 9836

culture collection no.: DSM 40833, JCM 4542, NRRL 2401, ATCC 33049, CGMCC 4.1992, CIP 108142, IFO 14657, MTCC 2532, NBIMCC 1591, NBRC 14657, VKM Ac-927, KCC S-0542

straininfo link

  • @ref: 85210
  • straininfo: 44936

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny23355697Streptomyces wuyuanensis sp. nov., an actinomycete from soil.Zhang X, Zhang J, Zheng J, Xin D, Xin Y, Pang HInt J Syst Evol Microbiol10.1099/ijs.0.047050-02013Bacterial Typing Techniques, Base Composition, China, DNA, Bacterial/genetics, Fatty Acids/analysis, Molecular Sequence Data, Nucleic Acid Hybridization, Phospholipids/analysis, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, *Soil Microbiology, Streptomyces/*classification/genetics/isolation & purification, Vitamin K 2/analogs & derivatives/analysisGenetics
Metabolism27373069[Genetic manipulation system and genomic library of Streptomyces luteosporeus NRRL 2401].Cheng X, Zhu T, Deng Z, You DWei Sheng Wu Xue Bao2016Bacterial Proteins/genetics/metabolism, Cloning, Molecular, Conjugation, Genetic, Escherichia coli/genetics, Gene Library, Genomic Library, Plasmids/genetics/metabolism, Streptomyces/*genetics/metabolismEnzymology

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
9836Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 40833)https://www.dsmz.de/collection/catalogue/details/culture/DSM-40833
19490Wink, J.http://www.dsmz.de/microorganisms/wink_pdf/DSM40833.pdfCompendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
33205Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/5732
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68368Automatically annotated from API 20E
68382Automatically annotated from API zym
85210Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID44936.1StrainInfo: A central database for resolving microbial strain identifiers
120696Curators of the CIPCollection of Institut Pasteur (CIP 108142)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20108142