Strain identifier

BacDive ID: 160069

Type strain: No

Species: Anaerostipes hadrus

Strain Designation: SS 2/1

Strain history: S. H. Duncan; Rowett Inst. of Nutrition and Health, UK; SS2/1.

NCBI tax ID(s): 649756 (species)

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General

@ref: 66973

BacDive-ID: 160069

DSM-Number: 23942

keywords: 16S sequence, Bacteria, mesophilic

description: Anaerostipes hadrus SS 2/1 is a mesophilic bacterium that was isolated from human faecal sample.

NCBI tax id

  • NCBI tax id: 649756
  • Matching level: species

strain history

@refhistory
66973<- S. H. Duncan, Rowett Inst. Nutrition and Health, Aberdeen, United Kingdom; SS 2/1
67770S. H. Duncan; Rowett Inst. of Nutrition and Health, UK; SS2/1.

doi: 10.13145/bacdive160069.20241212.9.2

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Clostridia
  • order: Eubacteriales
  • family: Lachnospiraceae
  • genus: Anaerostipes
  • species: Anaerostipes hadrus
  • full scientific name: Anaerostipes hadrus (Moore et al. 1976) Allen-Vercoe et al. 2013

  • synonyms

    • @ref: 20215
    • synonym: Eubacterium hadrum

@ref: 66973

domain: Bacteria

phylum: Firmicutes

class: Clostridia

order: Eubacteriales

family: Lachnospiraceae

genus: Anaerostipes

species: Anaerostipes hadrus

full scientific name: Anaerostipes hadrus (Moore et al. 1976) Allen-Vercoe et al. 2013

strain designation: SS 2/1

type strain: no

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
66973YCFA-MEDIUM (MODIFIED) (DSMZ Medium 1611)yeshttps://mediadive.dsmz.de/medium/1611Name: YCFA MEDIUM (modified) (DSMZ Medium 1611) Composition: Casitone 10.0 g/l Glucose 5.0 g/l NaHCO3 4.0 g/l Yeast extract 2.5 g/l Acetic acid 1.78695 g/l L-Cysteine HCl 1.0 g/l NaCl 0.9 g/l Propionic acid 0.62559 g/l KH2PO4 0.45 g/l K2HPO4 0.45 g/l CaCl2 x 2 H2O 0.09 g/l n-Valeric acid 0.08451 g/l iso-Valeric acid 0.0837899 g/l iso-Butyric acid 0.07695 g/l MgSO4 x 7 H2O 0.045 g/l Hemin 0.01 g/l Resazurin 0.001 g/l Pyridoxine hydrochloride 0.0001 g/l Nicotinic acid 5e-05 g/l Thiamine-HCl x 2 H2O 5e-05 g/l Riboflavin 5e-05 g/l D-Calcium pantothenate 5e-05 g/l p-Aminobenzoic acid 5e-05 g/l Lipoic acid 5e-05 g/l Folic acid 2e-05 g/l Biotin 2e-05 g/l Vitamin B12 1e-06 g/l Distilled water
66973FASTIDIOUS ANAEROBE AGAR (DSMZ Medium 1203)yeshttps://mediadive.dsmz.de/medium/1203Name: FASTIDIOUS ANAEROBE AGAR (DSMZ Medium 1203) Composition: Horse blood 100.0 g/l Fastidious Anaerobe Agar 45.7 g/l Distilled water

culture temp

@refgrowthtypetemperature
66973positivegrowth37
67770positivegrowth37

Physiology and metabolism

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6838027897tryptophan-energy source
6838017632nitrate-reduction
6838016634raffinose-fermentation
6838016024D-mannose-fermentation
6838029016arginine-hydrolysis
6838016199urea-hydrolysis

metabolite production

  • @ref: 68380
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

metabolite tests

  • @ref: 68380
  • Chebi-ID: 35581
  • metabolite: indole
  • indole test: -

enzymes

@refvalueactivityec
68380serine arylamidase-
68380glutamyl-glutamate arylamidase-
68380histidine arylamidase-
68380glycin arylamidase-
68380tyrosine arylamidase-
68380pyrrolidonyl arylamidase-3.4.19.3
68380leucine arylamidase-3.4.11.1
68380phenylalanine arylamidase-
68380leucyl glycin arylamidase-3.4.11.1
68380proline-arylamidase-3.4.11.5
68380L-arginine arylamidase-
68380tryptophan deaminase-4.1.99.1
68380alpha-fucosidase-3.2.1.51
68380beta-glucuronidase-3.2.1.31
68380alpha-arabinosidase-3.2.1.55
68380beta-glucosidase-3.2.1.21
68380alpha-galactosidase-3.2.1.22
68380arginine dihydrolase-3.5.3.6
68380urease-3.5.1.5
68380alanine arylamidase-3.4.11.2
68380N-acetyl-beta-glucosaminidase-3.2.1.52
68380beta-galactosidase+3.2.1.23

API rID32A

@refUREADH Argalpha GALbeta GALbeta GPalpha GLUbeta GLUalpha ARAbeta GURbeta NAGMNERAFGDCalpha FUCNITINDPALArgAProALGAPheALeuAPyrATyrAAlaAGlyAHisAGGASerA
66973---++/-+/-------+/----+/-------------

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinent
66973human faecal sampleAberdeenUnited KingdomGBREurope
67770Feces of a healthy British female

taxonmaps

  • @ref: 69479
  • File name: preview.99_2505.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_33;96_1350;97_1603;98_1949;99_2505&stattab=map
  • Last taxonomy: Anaerostipes hadrus subclade
  • 16S sequence: AY305319
  • Sequence Identity:
  • Total samples: 55629
  • soil counts: 188
  • aquatic counts: 983
  • animal counts: 54372
  • plant counts: 86

Safety information

risk assessment

  • @ref: 66973
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 66973
  • description: Anaerostipes hadrus 16S ribosomal RNA gene, partial sequence
  • accession: AY305319
  • length: 1455
  • database: nuccore
  • NCBI tax ID: 649756

GC content

  • @ref: 67770
  • GC-content: 41.8
  • method: high performance liquid chromatography (HPLC)

External links

@ref: 66973

culture collection no.: DSM 23942, JCM 17764

straininfo link

  • @ref: 112346
  • straininfo: 399428

Reference

@idauthorstitledoi/urlcatalogue
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
66973Curators of the DSMZhttps://www.dsmz.de/collection/catalogue/details/culture/DSM-23942Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 23942)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68380Automatically annotated from API rID32A
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.MicrobeAtlas 1.0 betahttps://microbeatlas.org/
112346Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.StrainInfo: A central database for resolving microbial strain identifiers10.60712/SI-ID399428.1