Strain identifier

BacDive ID: 160

Type strain: Yes

Species: Buchananella hordeovulneris

Strain history: CIP <- 1988, ATCC <- A.M. Buchanan: strain UCD 332-981

NCBI tax ID(s): 52770 (species)

For citation purpose refer to the digital object identifier (doi) of the current version.
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General

@ref: 9042

BacDive-ID: 160

DSM-Number: 20732

keywords: genome sequence, 16S sequence, Bacteria, microaerophile, mesophilic, rod-shaped, animal pathogen

description: Buchananella hordeovulneris DSM 20732 is a microaerophile, mesophilic, rod-shaped animal pathogen that was isolated from canine ascites fluid.

NCBI tax id

  • NCBI tax id: 52770
  • Matching level: species

strain history

@refhistory
9042<- ATCC <- A.M. Buchanan, UCD 81-332-9
119966CIP <- 1988, ATCC <- A.M. Buchanan: strain UCD 332-981

doi: 10.13145/bacdive160.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Actinomycetales
  • family: Actinomycetaceae
  • genus: Buchananella
  • species: Buchananella hordeovulneris
  • full scientific name: Buchananella hordeovulneris (Buchanan et al. 1984) Nouioui et al. 2018
  • synonyms

    • @ref: 20215
    • synonym: Actinomyces hordeovulneris

@ref: 9042

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Actinomycetaceae

genus: Buchananella

species: Buchananella hordeovulneris

full scientific name: Buchananella hordeovulneris (Buchanan et al. 1984) Nouioui et al. 2018

type strain: yes

Morphology

cell morphology

@refcell shapegram stainconfidencemotility
23107rod-shaped
69480positive100
119966rod-shapedpositiveno

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
9042PYG MEDIUM (MODIFIED) (DSMZ Medium 104)yeshttps://mediadive.dsmz.de/medium/104Name: PYG MEDIUM (modified) (DSMZ Medium 104) Composition: Yeast extract 10.0 g/l Peptone 5.0 g/l Trypticase peptone 5.0 g/l Beef extract 5.0 g/l Glucose 5.0 g/l L-Cysteine HCl x H2O 0.5 g/l NaHCO3 0.4 g/l NaCl 0.08 g/l K2HPO4 0.04 g/l KH2PO4 0.04 g/l MgSO4 x 7 H2O 0.02 g/l CaCl2 x 2 H2O 0.01 g/l Hemin 0.005 g/l Ethanol 0.0038 g/l Resazurin 0.001 g/l Tween 80 Vitamin K1 NaOH Distilled water
23107blood agar and 5% bovine blood agar and Trypticase soy or brain heart infusion agaryes
40541MEDIUM 17 - for Actinomyces hordeovulnerisyesDistilled water make up to (1000.000 ml);Magnesium sulphate heptahydrate (0.200 g);Calcium chloride dihydrate (0.020 g);Agar (15.000 g);Glucose (5.000 g);Yeast extract (5.000 g);Ammonium sulphate (1.000 g);Casitone (4.000 g);Starch maize (1.000 g);Heart i
119966CIP Medium 17yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=17

culture temp

@refgrowthtypetemperaturerange
9042positivegrowth37mesophilic
40541positivegrowth37mesophilic

Physiology and metabolism

oxygen tolerance

@refoxygen toleranceconfidence
9042microaerophile
23107anaerobe
69480anaerobe91.301
119966facultative anaerobe

spore formation

  • @ref: 69480
  • spore formation: no
  • confidence: 99.965

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
2310722599arabinose-builds acid from
2310717754glycerol-builds acid from
2310729864mannitol-builds acid from
2310717268myo-inositol-builds acid from
2310726546rhamnose-builds acid from
2310733942ribose-builds acid from
2310717057cellobiose+builds acid from
2310717234glucose+builds acid from
2310717716lactose+builds acid from
2310717306maltose+builds acid from
2310727082trehalose+builds acid from
2310718222xylose+builds acid from
11996617632nitrate-reduction
11996616301nitrite-reduction
6838016199urea-hydrolysis
6838029016arginine-hydrolysis
6838016024D-mannose-fermentation
6838016634raffinose-fermentation
6838029985L-glutamate-degradation
6838017632nitrate-reduction
6838027897tryptophan-energy source

antibiotic resistance

@refChEBImetaboliteis antibioticis sensitivesensitivity conc.
231073542cephalothinyesyes0.25 µg/mL
2310718208penicillin gyesyes0.25 µg/mL
2310717698chloramphenicolyesyes1 µg/mL
2310745924trimethoprimyesyes1 µg/mL

metabolite production

@refChebi-IDmetaboliteproduction
11996635581indoleno
6838035581indoleno

metabolite tests

  • @ref: 68380
  • Chebi-ID: 35581
  • metabolite: indole
  • indole test: -

enzymes

@refvalueactivityec
23107catalase+/-1.11.1.6
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase+3.2.1.52
68382beta-glucosidase-3.2.1.21
68382alpha-glucosidase-3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase+3.2.1.23
68382alpha-galactosidase+3.2.1.22
68382naphthol-AS-BI-phosphohydrolase-
68382acid phosphatase-3.1.3.2
68382alpha-chymotrypsin-3.4.21.1
68382trypsin-3.4.21.4
68382cystine arylamidase-3.4.11.3
68382valine arylamidase+
68382leucine arylamidase+3.4.11.1
68382lipase (C 14)-
68382esterase lipase (C 8)+
68382esterase (C 4)+
68382alkaline phosphatase-3.1.3.1
68380serine arylamidase+
68380glutamyl-glutamate arylamidase-
68380histidine arylamidase+
68380glycin arylamidase+
68380alanine arylamidase+3.4.11.2
68380tyrosine arylamidase+
68380leucine arylamidase+3.4.11.1
68380phenylalanine arylamidase+
68380leucyl glycin arylamidase+3.4.11.1
68380proline-arylamidase+3.4.11.5
68380L-arginine arylamidase+
68380alkaline phosphatase-3.1.3.1
68380tryptophan deaminase-4.1.99.1
68380alpha-fucosidase-3.2.1.51
68380glutamate decarboxylase-4.1.1.15
68380N-acetyl-beta-glucosaminidase+3.2.1.52
68380beta-glucuronidase-3.2.1.31
68380alpha-arabinosidase-3.2.1.55
68380beta-glucosidase+3.2.1.21
68380beta-Galactosidase 6-phosphate-
68380beta-galactosidase+3.2.1.23
68380alpha-galactosidase+3.2.1.22
68380arginine dihydrolase-3.5.3.6
68380urease-3.5.1.5
119966oxidase-
119966catalase-1.11.1.6
119966urease-3.5.1.5

fatty acid profile

  • fatty acids

    @reffatty acidpercentageECL
    51269C14:015.614
    51269C16:033.816
    51269C18:0518
    51269C13:0 ANTEISO1.312.701
    51269C14:0 ISO1.413.618
    51269C15:0 ANTEISO18.214.711
    51269C15:0 ISO3.114.621
    51269C16:0 iso2.815.626
    51269C17:0 anteiso2.816.722
    51269C18:1 ω9c8.217.769
    51269C18:2 ω6,9c/C18:0 ANTE817.724
  • type of FA analysis: whole cell analysis
  • method/protocol: CCUG

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
119966--++-++-----++---+--

API rID32A

@refUREADH Argalpha GALbeta GALbeta GPalpha GLUbeta GLUalpha ARAbeta GURbeta NAGMNERAFGDCalpha FUCNITINDPALArgAProALGAPheALeuAPyrATyrAAlaAGlyAHisAGGASerA
9042--++-+/-+--+-------++++++/-++++-+

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinent
9042canine ascites fluidCaliforniaUSAUSANorth America
51269Canine ascites fluid
119966Animal, Canine, ascites fluid

isolation source categories

Cat1Cat2Cat3
#Host#Mammals#Canidae (Dog)
#Host Body-Site#Gastrointestinal tract#Stomach
#Host Body Product#Fluids

taxonmaps

  • @ref: 69479
  • File name: preview.99_7521.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_224;96_3509;97_4304;98_5511;99_7521&stattab=map
  • Last taxonomy: Buchananella hordeovulneris subclade
  • 16S sequence: X82448
  • Sequence Identity:
  • Total samples: 4111
  • soil counts: 234
  • aquatic counts: 122
  • animal counts: 3650
  • plant counts: 105

Safety information

risk assessment

@refpathogenicity animalbiosafety levelbiosafety level comment
9042yes2Risk group (German classification)
1199662Risk group (French classification)

Sequence information

16S sequences

  • @ref: 20218
  • description: A.hordeovulneris 16S rRNA gene
  • accession: X82448
  • length: 1426
  • database: ena
  • NCBI tax ID: 52770

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Buchananella hordeovulneris DSM 20732GCA_001907235scaffoldncbi52770
66792Actinomyces hordeovulneris strain DSM 2073252770.4wgspatric52770
66792Actinomyces hordeovulneris DSM 207322857276557draftimg52770

GC content

  • @ref: 9042
  • GC-content: 67.0
  • method: Buoyant density centrifugation (BD)

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingyes66no
flagellatedno97.415no
gram-positiveyes94.388no
anaerobicno73.793no
aerobicno94.723no
halophileno85.234no
spore-formingno91.435no
glucose-utilyes82.834no
thermophileno93.59no
motileno91.312no
glucose-fermentyes66.321no

External links

@ref: 9042

culture collection no.: DSM 20732, ATCC 35275, CIP 103149, CCUG 32937, CIP 103127, UCD 81-332-9, UCD 332-981

straininfo link

  • @ref: 69842
  • straininfo: 92969

literature

  • topic: Phylogeny
  • Pubmed-ID: 6524750
  • title: Actinomyces hordeovulneris, a canine pathogen that produces L-phase variants spontaneously with coincident calcium deposition.
  • authors: Buchanan AM, Scott JL
  • journal: Am J Vet Res
  • year: 1984
  • mesh: Actinomyces/classification/*cytology/ultrastructure, Animals, Ascitic Fluid/microbiology/*veterinary, Calcium/metabolism, Dog Diseases/*microbiology, Dogs, L Forms/*cytology/ultrastructure, Microscopy, Electron
  • topic2: Metabolism

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
9042Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 20732)https://www.dsmz.de/collection/catalogue/details/culture/DSM-20732
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
23107Audria M. Buchanan, Jamon L. Scott, Mary Ann Gerencser, Blaine L. Beaman, Spencer Jang, Ernst L. Biberstein10.1099/00207713-34-4-439Actinomyces hordeovulneris sp. nov., an Agent of Canine ActinomycosisIJSEM 34: 439-443 1984
40541Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/14915
51269Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 32937)https://www.ccug.se/strain?id=32937
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
68380Automatically annotated from API rID32A
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69842Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID92969.1StrainInfo: A central database for resolving microbial strain identifiers
119966Curators of the CIPCollection of Institut Pasteur (CIP 103149)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20103149