Strain identifier
BacDive ID: 160
Type strain:
Species: Buchananella hordeovulneris
Strain history: CIP <- 1988, ATCC <- A.M. Buchanan: strain UCD 332-981
NCBI tax ID(s): 52770 (species)
General
@ref: 9042
BacDive-ID: 160
DSM-Number: 20732
keywords: genome sequence, 16S sequence, Bacteria, microaerophile, mesophilic, rod-shaped, animal pathogen
description: Buchananella hordeovulneris DSM 20732 is a microaerophile, mesophilic, rod-shaped animal pathogen that was isolated from canine ascites fluid.
NCBI tax id
- NCBI tax id: 52770
- Matching level: species
strain history
@ref | history |
---|---|
9042 | <- ATCC <- A.M. Buchanan, UCD 81-332-9 |
119966 | CIP <- 1988, ATCC <- A.M. Buchanan: strain UCD 332-981 |
doi: 10.13145/bacdive160.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/actinomycetota
- domain: Bacteria
- phylum: Actinomycetota
- class: Actinomycetes
- order: Actinomycetales
- family: Actinomycetaceae
- genus: Buchananella
- species: Buchananella hordeovulneris
- full scientific name: Buchananella hordeovulneris (Buchanan et al. 1984) Nouioui et al. 2018
synonyms
- @ref: 20215
- synonym: Actinomyces hordeovulneris
@ref: 9042
domain: Bacteria
phylum: Actinobacteria
class: Actinobacteria
order: Actinomycetales
family: Actinomycetaceae
genus: Buchananella
species: Buchananella hordeovulneris
full scientific name: Buchananella hordeovulneris (Buchanan et al. 1984) Nouioui et al. 2018
type strain: yes
Morphology
cell morphology
@ref | cell shape | gram stain | confidence | motility |
---|---|---|---|---|
23107 | rod-shaped | |||
69480 | positive | 100 | ||
119966 | rod-shaped | positive | no |
Culture and growth conditions
culture medium
@ref | name | growth | link | composition |
---|---|---|---|---|
9042 | PYG MEDIUM (MODIFIED) (DSMZ Medium 104) | yes | https://mediadive.dsmz.de/medium/104 | Name: PYG MEDIUM (modified) (DSMZ Medium 104) Composition: Yeast extract 10.0 g/l Peptone 5.0 g/l Trypticase peptone 5.0 g/l Beef extract 5.0 g/l Glucose 5.0 g/l L-Cysteine HCl x H2O 0.5 g/l NaHCO3 0.4 g/l NaCl 0.08 g/l K2HPO4 0.04 g/l KH2PO4 0.04 g/l MgSO4 x 7 H2O 0.02 g/l CaCl2 x 2 H2O 0.01 g/l Hemin 0.005 g/l Ethanol 0.0038 g/l Resazurin 0.001 g/l Tween 80 Vitamin K1 NaOH Distilled water |
23107 | blood agar and 5% bovine blood agar and Trypticase soy or brain heart infusion agar | yes | ||
40541 | MEDIUM 17 - for Actinomyces hordeovulneris | yes | Distilled water make up to (1000.000 ml);Magnesium sulphate heptahydrate (0.200 g);Calcium chloride dihydrate (0.020 g);Agar (15.000 g);Glucose (5.000 g);Yeast extract (5.000 g);Ammonium sulphate (1.000 g);Casitone (4.000 g);Starch maize (1.000 g);Heart i | |
119966 | CIP Medium 17 | yes | https://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=17 |
culture temp
@ref | growth | type | temperature | range |
---|---|---|---|---|
9042 | positive | growth | 37 | mesophilic |
40541 | positive | growth | 37 | mesophilic |
Physiology and metabolism
oxygen tolerance
@ref | oxygen tolerance | confidence |
---|---|---|
9042 | microaerophile | |
23107 | anaerobe | |
69480 | anaerobe | 91.301 |
119966 | facultative anaerobe |
spore formation
- @ref: 69480
- spore formation: no
- confidence: 99.965
metabolite utilization
@ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
---|---|---|---|---|
23107 | 22599 | arabinose | - | builds acid from |
23107 | 17754 | glycerol | - | builds acid from |
23107 | 29864 | mannitol | - | builds acid from |
23107 | 17268 | myo-inositol | - | builds acid from |
23107 | 26546 | rhamnose | - | builds acid from |
23107 | 33942 | ribose | - | builds acid from |
23107 | 17057 | cellobiose | + | builds acid from |
23107 | 17234 | glucose | + | builds acid from |
23107 | 17716 | lactose | + | builds acid from |
23107 | 17306 | maltose | + | builds acid from |
23107 | 27082 | trehalose | + | builds acid from |
23107 | 18222 | xylose | + | builds acid from |
119966 | 17632 | nitrate | - | reduction |
119966 | 16301 | nitrite | - | reduction |
68380 | 16199 | urea | - | hydrolysis |
68380 | 29016 | arginine | - | hydrolysis |
68380 | 16024 | D-mannose | - | fermentation |
68380 | 16634 | raffinose | - | fermentation |
68380 | 29985 | L-glutamate | - | degradation |
68380 | 17632 | nitrate | - | reduction |
68380 | 27897 | tryptophan | - | energy source |
antibiotic resistance
@ref | ChEBI | metabolite | is antibiotic | is sensitive | sensitivity conc. |
---|---|---|---|---|---|
23107 | 3542 | cephalothin | yes | yes | 0.25 µg/mL |
23107 | 18208 | penicillin g | yes | yes | 0.25 µg/mL |
23107 | 17698 | chloramphenicol | yes | yes | 1 µg/mL |
23107 | 45924 | trimethoprim | yes | yes | 1 µg/mL |
metabolite production
@ref | Chebi-ID | metabolite | production |
---|---|---|---|
119966 | 35581 | indole | no |
68380 | 35581 | indole | no |
metabolite tests
- @ref: 68380
- Chebi-ID: 35581
- metabolite: indole
- indole test: -
enzymes
@ref | value | activity | ec |
---|---|---|---|
23107 | catalase | +/- | 1.11.1.6 |
68382 | alpha-fucosidase | - | 3.2.1.51 |
68382 | alpha-mannosidase | - | 3.2.1.24 |
68382 | N-acetyl-beta-glucosaminidase | + | 3.2.1.52 |
68382 | beta-glucosidase | - | 3.2.1.21 |
68382 | alpha-glucosidase | - | 3.2.1.20 |
68382 | beta-glucuronidase | - | 3.2.1.31 |
68382 | beta-galactosidase | + | 3.2.1.23 |
68382 | alpha-galactosidase | + | 3.2.1.22 |
68382 | naphthol-AS-BI-phosphohydrolase | - | |
68382 | acid phosphatase | - | 3.1.3.2 |
68382 | alpha-chymotrypsin | - | 3.4.21.1 |
68382 | trypsin | - | 3.4.21.4 |
68382 | cystine arylamidase | - | 3.4.11.3 |
68382 | valine arylamidase | + | |
68382 | leucine arylamidase | + | 3.4.11.1 |
68382 | lipase (C 14) | - | |
68382 | esterase lipase (C 8) | + | |
68382 | esterase (C 4) | + | |
68382 | alkaline phosphatase | - | 3.1.3.1 |
68380 | serine arylamidase | + | |
68380 | glutamyl-glutamate arylamidase | - | |
68380 | histidine arylamidase | + | |
68380 | glycin arylamidase | + | |
68380 | alanine arylamidase | + | 3.4.11.2 |
68380 | tyrosine arylamidase | + | |
68380 | leucine arylamidase | + | 3.4.11.1 |
68380 | phenylalanine arylamidase | + | |
68380 | leucyl glycin arylamidase | + | 3.4.11.1 |
68380 | proline-arylamidase | + | 3.4.11.5 |
68380 | L-arginine arylamidase | + | |
68380 | alkaline phosphatase | - | 3.1.3.1 |
68380 | tryptophan deaminase | - | 4.1.99.1 |
68380 | alpha-fucosidase | - | 3.2.1.51 |
68380 | glutamate decarboxylase | - | 4.1.1.15 |
68380 | N-acetyl-beta-glucosaminidase | + | 3.2.1.52 |
68380 | beta-glucuronidase | - | 3.2.1.31 |
68380 | alpha-arabinosidase | - | 3.2.1.55 |
68380 | beta-glucosidase | + | 3.2.1.21 |
68380 | beta-Galactosidase 6-phosphate | - | |
68380 | beta-galactosidase | + | 3.2.1.23 |
68380 | alpha-galactosidase | + | 3.2.1.22 |
68380 | arginine dihydrolase | - | 3.5.3.6 |
68380 | urease | - | 3.5.1.5 |
119966 | oxidase | - | |
119966 | catalase | - | 1.11.1.6 |
119966 | urease | - | 3.5.1.5 |
fatty acid profile
fatty acids
@ref fatty acid percentage ECL 51269 C14:0 15.6 14 51269 C16:0 33.8 16 51269 C18:0 5 18 51269 C13:0 ANTEISO 1.3 12.701 51269 C14:0 ISO 1.4 13.618 51269 C15:0 ANTEISO 18.2 14.711 51269 C15:0 ISO 3.1 14.621 51269 C16:0 iso 2.8 15.626 51269 C17:0 anteiso 2.8 16.722 51269 C18:1 ω9c 8.2 17.769 51269 C18:2 ω6,9c/C18:0 ANTE 8 17.724 - type of FA analysis: whole cell analysis
- method/protocol: CCUG
API zym
@ref | Control | Alkaline phosphatase | Esterase | Esterase Lipase | Lipase | Leucine arylamidase | Valine arylamidase | Cystine arylamidase | Trypsin | alpha- Chymotrypsin | Acid phosphatase | Naphthol-AS-BI-phosphohydrolase | alpha- Galactosidase | beta- Galactosidase | beta- Glucuronidase | alpha- Glucosidase | beta- Glucosidase | N-acetyl-beta- glucosaminidase | alpha- Mannosidase | alpha- Fucosidase |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
119966 | - | - | + | + | - | + | + | - | - | - | - | - | + | + | - | - | - | + | - | - |
API rID32A
@ref | URE | ADH Arg | alpha GAL | beta GAL | beta GP | alpha GLU | beta GLU | alpha ARA | beta GUR | beta NAG | MNE | RAF | GDC | alpha FUC | NIT | IND | PAL | ArgA | ProA | LGA | PheA | LeuA | PyrA | TyrA | AlaA | GlyA | HisA | GGA | SerA |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
9042 | - | - | + | + | - | +/- | + | - | - | + | - | - | - | - | - | - | - | + | + | + | + | + | +/- | + | + | + | + | - | + |
Isolation, sampling and environmental information
isolation
@ref | sample type | geographic location | country | origin.country | continent |
---|---|---|---|---|---|
9042 | canine ascites fluid | California | USA | USA | North America |
51269 | Canine ascites fluid | ||||
119966 | Animal, Canine, ascites fluid |
isolation source categories
Cat1 | Cat2 | Cat3 |
---|---|---|
#Host | #Mammals | #Canidae (Dog) |
#Host Body-Site | #Gastrointestinal tract | #Stomach |
#Host Body Product | #Fluids |
taxonmaps
- @ref: 69479
- File name: preview.99_7521.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_224;96_3509;97_4304;98_5511;99_7521&stattab=map
- Last taxonomy: Buchananella hordeovulneris subclade
- 16S sequence: X82448
- Sequence Identity:
- Total samples: 4111
- soil counts: 234
- aquatic counts: 122
- animal counts: 3650
- plant counts: 105
Safety information
risk assessment
@ref | pathogenicity animal | biosafety level | biosafety level comment |
---|---|---|---|
9042 | yes | 2 | Risk group (German classification) |
119966 | 2 | Risk group (French classification) |
Sequence information
16S sequences
- @ref: 20218
- description: A.hordeovulneris 16S rRNA gene
- accession: X82448
- length: 1426
- database: ena
- NCBI tax ID: 52770
Genome sequences
@ref | description | accession | assembly level | database | NCBI tax ID |
---|---|---|---|---|---|
66792 | Buchananella hordeovulneris DSM 20732 | GCA_001907235 | scaffold | ncbi | 52770 |
66792 | Actinomyces hordeovulneris strain DSM 20732 | 52770.4 | wgs | patric | 52770 |
66792 | Actinomyces hordeovulneris DSM 20732 | 2857276557 | draft | img | 52770 |
GC content
- @ref: 9042
- GC-content: 67.0
- method: Buoyant density centrifugation (BD)
Genome-based predictions
predictions
trait | prediction | confidence | training_data |
---|---|---|---|
spore-forming | yes | 66 | no |
flagellated | no | 97.415 | no |
gram-positive | yes | 94.388 | no |
anaerobic | no | 73.793 | no |
aerobic | no | 94.723 | no |
halophile | no | 85.234 | no |
spore-forming | no | 91.435 | no |
glucose-util | yes | 82.834 | no |
thermophile | no | 93.59 | no |
motile | no | 91.312 | no |
glucose-ferment | yes | 66.321 | no |
External links
@ref: 9042
culture collection no.: DSM 20732, ATCC 35275, CIP 103149, CCUG 32937, CIP 103127, UCD 81-332-9, UCD 332-981
straininfo link
- @ref: 69842
- straininfo: 92969
literature
- topic: Phylogeny
- Pubmed-ID: 6524750
- title: Actinomyces hordeovulneris, a canine pathogen that produces L-phase variants spontaneously with coincident calcium deposition.
- authors: Buchanan AM, Scott JL
- journal: Am J Vet Res
- year: 1984
- mesh: Actinomyces/classification/*cytology/ultrastructure, Animals, Ascitic Fluid/microbiology/*veterinary, Calcium/metabolism, Dog Diseases/*microbiology, Dogs, L Forms/*cytology/ultrastructure, Microscopy, Electron
- topic2: Metabolism
Reference
@id | authors | catalogue | doi/url | title | journal | pubmed |
---|---|---|---|---|---|---|
9042 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 20732) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-20732 | |||
20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |||
20218 | Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P. | 10.1016/j.syapm.2013.11.002 | StrainInfo introduces electronic passports for microorganisms. | Syst Appl Microbiol. 37: 42-50 2014 | 24321274 | |
23107 | Audria M. Buchanan, Jamon L. Scott, Mary Ann Gerencser, Blaine L. Beaman, Spencer Jang, Ernst L. Biberstein | 10.1099/00207713-34-4-439 | Actinomyces hordeovulneris sp. nov., an Agent of Canine Actinomycosis | IJSEM 34: 439-443 1984 | ||
40541 | Curators of the CIP | https://brclims.pasteur.fr/brcWeb/souche/detail/1/14915 | ||||
51269 | Curators of the CCUG | Culture Collection University of Gothenburg (CCUG) (CCUG 32937) | https://www.ccug.se/strain?id=32937 | |||
66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |||
68380 | Automatically annotated from API rID32A | |||||
68382 | Automatically annotated from API zym | |||||
69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | https://microbeatlas.org/ | MicrobeAtlas 1.0 beta | |||
69480 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |||
69842 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID92969.1 | StrainInfo: A central database for resolving microbial strain identifiers | |||
119966 | Curators of the CIP | Collection of Institut Pasteur (CIP 103149) | https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20103149 |