Strain identifier

BacDive ID: 16

Type strain: Yes

Species: Acetobacter orientalis

Strain Designation: 21F-2

Strain history: CIP <- 2002, JCM <- 2001, NRIC <- P. Lisdiyanti: strain 21F-2

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General

@ref: 5996

BacDive-ID: 16

DSM-Number: 15550

keywords: genome sequence, 16S sequence, Bacteria, obligate aerobe, mesophilic, Gram-negative, rod-shaped

description: Acetobacter orientalis 21F-2 is an obligate aerobe, mesophilic, Gram-negative bacterium that was isolated from canna flower, Canna hybrida.

NCBI tax id

NCBI tax idMatching level
1307913strain
146474species

strain history

@refhistory
5996<- IFO
67770NRIC 0481 <-- P. Lisdiyanti 21F-2.
121435CIP <- 2002, JCM <- 2001, NRIC <- P. Lisdiyanti: strain 21F-2

doi: 10.13145/bacdive16.20241212.9.2

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Alphaproteobacteria
  • order: Rhodospirillales
  • family: Acetobacteraceae
  • genus: Acetobacter
  • species: Acetobacter orientalis
  • full scientific name: Acetobacter orientalis Lisdiyanti et al. 2002

@ref: 5996

domain: Bacteria

phylum: Proteobacteria

class: Alphaproteobacteria

order: Rhizobiales

family: Acetobacteraceae

genus: Acetobacter

species: Acetobacter orientalis

full scientific name: Acetobacter orientalis Lisdiyanti et al. 2002

strain designation: 21F-2

type strain: yes

Morphology

cell morphology

@refgram staincell shapemotilityconfidence
121435negativerod-shapedno
69480negative95.833

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
5996ACETIC ACID BACTERIUM MEDIUM (DSMZ Medium 989)yeshttps://mediadive.dsmz.de/medium/989Name: ACETIC ACID BACTERIUM MEDIUM (DSMZ Medium 989) Composition: Agar 15.0 g/l Glucose 5.0 g/l Yeast extract 5.0 g/l Bacto peptone 5.0 g/l MgSO4 x 7 H2O 1.0 g/l Distilled water
33782MEDIUM 72- for trypto casein soja agaryesDistilled water make up to (1000.000 ml);Trypto casein soy agar (40.000 g)
121435CIP Medium 72yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=72

culture temp

@refgrowthtypetemperature
5996positivegrowth30
33782positivegrowth30
67770positivegrowth30
121435positivegrowth22-37
121435nogrowth10
121435nogrowth41
121435nogrowth45

Physiology and metabolism

oxygen tolerance

  • @ref: 121435
  • oxygen tolerance: obligate aerobe

spore formation

  • @ref: 69480
  • spore formation: no
  • confidence: 92.965

halophily

@refsaltgrowthtested relationconcentration
121435NaClnogrowth0 %
121435NaClnogrowth2 %
121435NaClnogrowth4 %
121435NaClnogrowth6 %
121435NaClnogrowth8 %
121435NaClnogrowth10 %

observation

  • @ref: 67770
  • observation: quinones: Q-9

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
121435606565hippurate+hydrolysis
12143517632nitrate-reduction
12143516301nitrite-reduction

antibiotic resistance

  • @ref: 121435
  • metabolite: 0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)
  • is antibiotic: yes
  • is sensitive: no
  • is resistant: yes

metabolite production

  • @ref: 121435
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

enzymes

@refvalueactivityec
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-glucosidase-3.2.1.20
68382beta-glucosidase-3.2.1.21
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase-3.2.1.23
68382naphthol-AS-BI-phosphohydrolase-
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase-3.1.3.2
68382cystine arylamidase-3.4.11.3
68382leucine arylamidase+3.4.11.1
68382valine arylamidase-
68382lipase (C 14)-
68382esterase (C 4)+
68382esterase lipase (C 8)-
68382alkaline phosphatase-3.1.3.1
121435oxidase-
121435beta-galactosidase-3.2.1.23
121435gelatinase-
121435catalase+1.11.1.6
121435gamma-glutamyltransferase-2.3.2.2
121435urease-3.5.1.5
68382alpha-galactosidase-3.2.1.22
68382trypsin-3.4.21.4

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
121435--+--+--------------

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
121435--------------------------------------------------------------+--+--------------------+------------

Isolation, sampling and environmental information

isolation

@refsample typehost speciescountryorigin.countrycontinentisolation date
5996canna flower, Canna hybridaCanna hybridaIndonesiaIDNAsia
67770Canna flower (Canna hybrida)Canna hybridaIndonesiaIDNAsia
121435Canna flowerIndonesiaIDNAsia1996

isolation source categories

Cat1Cat2Cat3
#Host#Plants#Herbaceous plants (Grass,Crops)
#Host Body-Site#Plant#Flower

taxonmaps

  • @ref: 69479
  • File name: preview.99_5543.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_29;96_152;97_827;98_961;99_5543&stattab=map
  • Last taxonomy: Acetobacter orientalis
  • 16S sequence: AB681086
  • Sequence Identity:
  • Total samples: 1058
  • soil counts: 157
  • aquatic counts: 68
  • animal counts: 624
  • plant counts: 209

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
59961Risk group (German classification)
1214351Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Acetobacter orientalis gene for 16S rRNA, partial sequence, strain: NBRC 16606AB6810861415nuccore146474
5996Acetobacter orientalis gene for 16S ribosomal RNA, strain: 21F-2AB0527061401nuccore146474

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Acetobacter orientalis 21F-21231341.3wgspatric1307913
66792Acetobacter orientalis strain NBRC 16606146474.10wgspatric146474
66792Acetobacter orientalis 21F-22899144674draftimg146474
67770Acetobacter orientalis 21F-2GCA_000963965scaffoldncbi146474
67770Acetobacter orientalis NBRC 16606GCA_007989335contigncbi146474

GC content

  • @ref: 67770
  • GC-content: 52.3
  • method: high performance liquid chromatography (HPLC)

Genome-based predictions

predictions

@reftraitmodeldescriptionpredictionconfidencetraining_data
69480gram-positivegram-positivePositive reaction to Gram-stainingno95.833no
69480anaerobicanaerobicAbility to grow under anoxygenic conditions (including facultative anaerobes)no88.783yes
69480spore-formingspore-formingAbility to form endo- or exosporesno92.965no
69480aerobicaerobicAbility to grow under oxygenic conditions (including facultative aerobes)yes82.825yes
69480thermophilicthermophileAbility to grow at temperatures above or equal to 45°Cno98.816yes
69480flagellatedmotile2+Ability to perform flagellated movementyes59.975no

External links

@ref: 5996

culture collection no.: DSM 15550, IFO 16606, NBRC 16606, NRIC 0481, JCM 11195, CIP 107379

straininfo link

  • @ref: 69700
  • straininfo: 265202

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
5996Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 15550)https://www.dsmz.de/collection/catalogue/details/culture/DSM-15550
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
33782Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/4808
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69700Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID265202.1StrainInfo: A central database for resolving microbial strain identifiers
121435Curators of the CIPCollection of Institut Pasteur (CIP 107379)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20107379