Strain identifier
BacDive ID: 16
Type strain:
Species: Acetobacter orientalis
Strain Designation: 21F-2
Strain history: CIP <- 2002, JCM <- 2001, NRIC <- P. Lisdiyanti: strain 21F-2
NCBI tax ID(s): 1307913 (strain), 146474 (species)
General
@ref: 5996
BacDive-ID: 16
DSM-Number: 15550
keywords: genome sequence, 16S sequence, Bacteria, obligate aerobe, mesophilic, Gram-negative, rod-shaped
description: Acetobacter orientalis 21F-2 is an obligate aerobe, mesophilic, Gram-negative bacterium that was isolated from canna flower, Canna hybrida.
NCBI tax id
NCBI tax id | Matching level |
---|---|
1307913 | strain |
146474 | species |
strain history
@ref | history |
---|---|
5996 | <- IFO |
67770 | NRIC 0481 <-- P. Lisdiyanti 21F-2. |
121435 | CIP <- 2002, JCM <- 2001, NRIC <- P. Lisdiyanti: strain 21F-2 |
doi: 10.13145/bacdive16.20241212.9.2
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/pseudomonadota
- domain: Bacteria
- phylum: Pseudomonadota
- class: Alphaproteobacteria
- order: Rhodospirillales
- family: Acetobacteraceae
- genus: Acetobacter
- species: Acetobacter orientalis
- full scientific name: Acetobacter orientalis Lisdiyanti et al. 2002
@ref: 5996
domain: Bacteria
phylum: Proteobacteria
class: Alphaproteobacteria
order: Rhizobiales
family: Acetobacteraceae
genus: Acetobacter
species: Acetobacter orientalis
full scientific name: Acetobacter orientalis Lisdiyanti et al. 2002
strain designation: 21F-2
type strain: yes
Morphology
cell morphology
@ref | gram stain | cell shape | motility | confidence |
---|---|---|---|---|
121435 | negative | rod-shaped | no | |
69480 | negative | 95.833 |
Culture and growth conditions
culture medium
@ref | name | growth | link | composition |
---|---|---|---|---|
5996 | ACETIC ACID BACTERIUM MEDIUM (DSMZ Medium 989) | yes | https://mediadive.dsmz.de/medium/989 | Name: ACETIC ACID BACTERIUM MEDIUM (DSMZ Medium 989) Composition: Agar 15.0 g/l Glucose 5.0 g/l Yeast extract 5.0 g/l Bacto peptone 5.0 g/l MgSO4 x 7 H2O 1.0 g/l Distilled water |
33782 | MEDIUM 72- for trypto casein soja agar | yes | Distilled water make up to (1000.000 ml);Trypto casein soy agar (40.000 g) | |
121435 | CIP Medium 72 | yes | https://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=72 |
culture temp
@ref | growth | type | temperature |
---|---|---|---|
5996 | positive | growth | 30 |
33782 | positive | growth | 30 |
67770 | positive | growth | 30 |
121435 | positive | growth | 22-37 |
121435 | no | growth | 10 |
121435 | no | growth | 41 |
121435 | no | growth | 45 |
Physiology and metabolism
oxygen tolerance
- @ref: 121435
- oxygen tolerance: obligate aerobe
spore formation
- @ref: 69480
- spore formation: no
- confidence: 92.965
halophily
@ref | salt | growth | tested relation | concentration |
---|---|---|---|---|
121435 | NaCl | no | growth | 0 % |
121435 | NaCl | no | growth | 2 % |
121435 | NaCl | no | growth | 4 % |
121435 | NaCl | no | growth | 6 % |
121435 | NaCl | no | growth | 8 % |
121435 | NaCl | no | growth | 10 % |
observation
- @ref: 67770
- observation: quinones: Q-9
metabolite utilization
@ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
---|---|---|---|---|
121435 | 606565 | hippurate | + | hydrolysis |
121435 | 17632 | nitrate | - | reduction |
121435 | 16301 | nitrite | - | reduction |
antibiotic resistance
- @ref: 121435
- metabolite: 0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)
- is antibiotic: yes
- is sensitive: no
- is resistant: yes
metabolite production
- @ref: 121435
- Chebi-ID: 35581
- metabolite: indole
- production: no
enzymes
@ref | value | activity | ec |
---|---|---|---|
68382 | alpha-fucosidase | - | 3.2.1.51 |
68382 | alpha-mannosidase | - | 3.2.1.24 |
68382 | N-acetyl-beta-glucosaminidase | - | 3.2.1.52 |
68382 | alpha-glucosidase | - | 3.2.1.20 |
68382 | beta-glucosidase | - | 3.2.1.21 |
68382 | beta-glucuronidase | - | 3.2.1.31 |
68382 | beta-galactosidase | - | 3.2.1.23 |
68382 | naphthol-AS-BI-phosphohydrolase | - | |
68382 | alpha-chymotrypsin | - | 3.4.21.1 |
68382 | acid phosphatase | - | 3.1.3.2 |
68382 | cystine arylamidase | - | 3.4.11.3 |
68382 | leucine arylamidase | + | 3.4.11.1 |
68382 | valine arylamidase | - | |
68382 | lipase (C 14) | - | |
68382 | esterase (C 4) | + | |
68382 | esterase lipase (C 8) | - | |
68382 | alkaline phosphatase | - | 3.1.3.1 |
121435 | oxidase | - | |
121435 | beta-galactosidase | - | 3.2.1.23 |
121435 | gelatinase | - | |
121435 | catalase | + | 1.11.1.6 |
121435 | gamma-glutamyltransferase | - | 2.3.2.2 |
121435 | urease | - | 3.5.1.5 |
68382 | alpha-galactosidase | - | 3.2.1.22 |
68382 | trypsin | - | 3.4.21.4 |
API zym
@ref | Control | Alkaline phosphatase | Esterase | Esterase Lipase | Lipase | Leucine arylamidase | Valine arylamidase | Cystine arylamidase | Trypsin | alpha- Chymotrypsin | Acid phosphatase | Naphthol-AS-BI-phosphohydrolase | alpha- Galactosidase | beta- Galactosidase | beta- Glucuronidase | alpha- Glucosidase | beta- Glucosidase | N-acetyl-beta- glucosaminidase | alpha- Mannosidase | alpha- Fucosidase |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
121435 | - | - | + | - | - | + | - | - | - | - | - | - | - | - | - | - | - | - | - | - |
API biotype100
@ref | GLU | FRU | GAL | TRE | MNE | SBE | MEL | SAC | RAF | MTE | MAL | LAC | LTE | MbGa | MaGa | CEL | GEN | MbGu | ESC | RIB | ARA | XYL | PLE | RHA | FUC | MLZ | DARL | LARL | XLT | DUL | TAG | GLY | INO | MAN | MTL | TUR | SOR | ADO | HBG | LYX | ERY | MDG | 3MDG | SAT | MUC | LTAT | DTAT | MTAT | DMLT | LMLT | CATE | TATE | TTE | CIT | GRT | GAT | 2KG | 5KG | TRY | NAG | GNT | PAC | PAT | pOBE | QAT | GTE | mOBE | BAT | PPAT | CMT | TGE | BET | PCE | ABT | HIN | LAT | CAP | CYT | HIS | SUC | FUM | GRE | GYT | AVT | ETN | TTN | GLN | ITA | 3OBU | APT | GTT | PRO | DALA | LALA | SER | MNT | PROP | TYR | 2KT |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
121435 | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | + | - | - | + | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | + | - | - | - | - | - | - | - | - | - | - | - | - |
Isolation, sampling and environmental information
isolation
@ref | sample type | host species | country | origin.country | continent | isolation date |
---|---|---|---|---|---|---|
5996 | canna flower, Canna hybrida | Canna hybrida | Indonesia | IDN | Asia | |
67770 | Canna flower (Canna hybrida) | Canna hybrida | Indonesia | IDN | Asia | |
121435 | Canna flower | Indonesia | IDN | Asia | 1996 |
isolation source categories
Cat1 | Cat2 | Cat3 |
---|---|---|
#Host | #Plants | #Herbaceous plants (Grass,Crops) |
#Host Body-Site | #Plant | #Flower |
taxonmaps
- @ref: 69479
- File name: preview.99_5543.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_29;96_152;97_827;98_961;99_5543&stattab=map
- Last taxonomy: Acetobacter orientalis
- 16S sequence: AB681086
- Sequence Identity:
- Total samples: 1058
- soil counts: 157
- aquatic counts: 68
- animal counts: 624
- plant counts: 209
Safety information
risk assessment
@ref | biosafety level | biosafety level comment |
---|---|---|
5996 | 1 | Risk group (German classification) |
121435 | 1 | Risk group (French classification) |
Sequence information
16S sequences
@ref | description | accession | length | database | NCBI tax ID |
---|---|---|---|---|---|
20218 | Acetobacter orientalis gene for 16S rRNA, partial sequence, strain: NBRC 16606 | AB681086 | 1415 | nuccore | 146474 |
5996 | Acetobacter orientalis gene for 16S ribosomal RNA, strain: 21F-2 | AB052706 | 1401 | nuccore | 146474 |
Genome sequences
@ref | description | accession | assembly level | database | NCBI tax ID |
---|---|---|---|---|---|
66792 | Acetobacter orientalis 21F-2 | 1231341.3 | wgs | patric | 1307913 |
66792 | Acetobacter orientalis strain NBRC 16606 | 146474.10 | wgs | patric | 146474 |
66792 | Acetobacter orientalis 21F-2 | 2899144674 | draft | img | 146474 |
67770 | Acetobacter orientalis 21F-2 | GCA_000963965 | scaffold | ncbi | 146474 |
67770 | Acetobacter orientalis NBRC 16606 | GCA_007989335 | contig | ncbi | 146474 |
GC content
- @ref: 67770
- GC-content: 52.3
- method: high performance liquid chromatography (HPLC)
Genome-based predictions
predictions
@ref | trait | model | description | prediction | confidence | training_data |
---|---|---|---|---|---|---|
69480 | gram-positive | gram-positive | Positive reaction to Gram-staining | no | 95.833 | no |
69480 | anaerobic | anaerobic | Ability to grow under anoxygenic conditions (including facultative anaerobes) | no | 88.783 | yes |
69480 | spore-forming | spore-forming | Ability to form endo- or exospores | no | 92.965 | no |
69480 | aerobic | aerobic | Ability to grow under oxygenic conditions (including facultative aerobes) | yes | 82.825 | yes |
69480 | thermophilic | thermophile | Ability to grow at temperatures above or equal to 45°C | no | 98.816 | yes |
69480 | flagellated | motile2+ | Ability to perform flagellated movement | yes | 59.975 | no |
External links
@ref: 5996
culture collection no.: DSM 15550, IFO 16606, NBRC 16606, NRIC 0481, JCM 11195, CIP 107379
straininfo link
- @ref: 69700
- straininfo: 265202
Reference
@id | authors | catalogue | doi/url | title | journal | pubmed |
---|---|---|---|---|---|---|
5996 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 15550) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-15550 | |||
20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |||
20218 | Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P. | 10.1016/j.syapm.2013.11.002 | StrainInfo introduces electronic passports for microorganisms. | Syst Appl Microbiol. 37: 42-50 2014 | 24321274 | |
33782 | Curators of the CIP | https://brclims.pasteur.fr/brcWeb/souche/detail/1/4808 | ||||
66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |||
67770 | Curators of the JCM | https://jcm.brc.riken.jp/en/ | ||||
68382 | Automatically annotated from API zym | |||||
69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | https://microbeatlas.org/ | MicrobeAtlas 1.0 beta | |||
69480 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |||
69700 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID265202.1 | StrainInfo: A central database for resolving microbial strain identifiers | |||
121435 | Curators of the CIP | Collection of Institut Pasteur (CIP 107379) | https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20107379 |