Strain identifier

BacDive ID: 159905

Type strain: Yes

Species: Tenuifilum thalassicum

Strain Designation: 38H-Str., 38H-str

Strain history: <- O. A. Podosokorskaya, Winogradsky Inst. Microbiol., RAS, Moscow, Russia; 38H-Str.

NCBI tax ID(s): 2590900 (species)

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General

@ref: 66809

BacDive-ID: 159905

DSM-Number: 100343

keywords: genome sequence, Bacteria, obligate anaerobe, halophilic, chemoorganoheterotroph, thermophilic, Gram-negative, motile, rod-shaped

description: Tenuifilum thalassicum 38H-Str. is an obligate anaerobe, halophilic, chemoorganoheterotroph bacterium that builds Filamentous rods and was isolated from mixture of iron-contining deposits, shallow hot spring.

NCBI tax id

  • NCBI tax id: 2590900
  • Matching level: species

strain history

  • @ref: 66809
  • history: <- O. A. Podosokorskaya, Winogradsky Inst. Microbiol., RAS, Moscow, Russia; 38H-Str.

doi: 10.13145/bacdive159905.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacteroidota
  • domain: Bacteria
  • phylum: Bacteroidota
  • class: Bacteroidia
  • order: Bacteroidales
  • family: Tenuifilaceae
  • genus: Tenuifilum
  • species: Tenuifilum thalassicum
  • full scientific name: Tenuifilum thalassicum Podosokorskaya et al. 2021

@ref: 66809

domain: Bacteria

phylum: Bacteroidetes

class: Bacteroidia

order: Bacteroidales

family: Tenuifilaceae

genus: Tenuifilum

species: Tenuifilum thalassicum

full scientific name: Tenuifilum thalassicum Podosokorskaya et al. 2020

strain designation: 38H-Str., 38H-str

type strain: yes

Morphology

cell morphology

  • @ref: 69634
  • gram stain: negative
  • cell length: 3-10 µm
  • cell width: 0.1-0.2 µm
  • cell shape: rod-shaped
  • motility: yes
  • flagellum arrangement: gliding

multicellular morphology

  • @ref: 69634
  • forms multicellular complex: yes
  • complex name: Filamentous rods
  • further description: sometimes forming loops, knots and rosettes

Culture and growth conditions

culture medium

  • @ref: 66809
  • name: PELLABORIMONAS MEDIUM (DSMZ Medium 1717)
  • growth: yes
  • link: https://mediadive.dsmz.de/medium/1717
  • composition: Name: PERLABENTIMONAS MEDIUM (DSMZ Medium 1717) Composition: NaHCO3 2.0 g/l Tryptone 2.0 g/l Fructose 2.0 g/l Na2S x 9 H2O 0.5 g/l KH2PO4 0.33 g/l NH4Cl 0.33 g/l KCl 0.33 g/l MgCl2 x 6 H2O 0.33 g/l Yeast extract 0.05 g/l CaCl2 x 6 H2O 0.033 g/l MgSO4 x 7 H2O 0.03 g/l Nitrilotriacetic acid 0.015 g/l NaCl 0.01 g/l MnSO4 x H2O 0.005 g/l ZnSO4 x 7 H2O 0.0018 g/l CoSO4 x 7 H2O 0.0018 g/l Resazurin 0.001 g/l CaCl2 x 2 H2O 0.001 g/l FeSO4 x 7 H2O 0.001 g/l NiCl2 x 6 H2O 0.0003 g/l AlK(SO4)2 x 12 H2O 0.0002 g/l H3BO3 0.0001 g/l Pyridoxine hydrochloride 0.0001 g/l CuSO4 x 5 H2O 0.0001 g/l Na2MoO4 x 2 H2O 0.0001 g/l (DL)-alpha-Lipoic acid 5e-05 g/l p-Aminobenzoic acid 5e-05 g/l Calcium D-(+)-pantothenate 5e-05 g/l Nicotinic acid 5e-05 g/l Riboflavin 5e-05 g/l Thiamine HCl 5e-05 g/l Folic acid 2e-05 g/l Biotin 2e-05 g/l Na2WO4 x 2 H2O 4e-06 g/l Na2SeO3 x 5 H2O 3e-06 g/l Vitamin B12 1e-06 g/l Distilled water

culture temp

@refgrowthtypetemperaturerange
66809positivegrowth50thermophilic
69634positivegrowth20-65
69634positiveoptimum50thermophilic

culture pH

@refabilitytypepH
69634positivegrowth4-9
69634positiveoptimum6.5-7

Physiology and metabolism

oxygen tolerance

  • @ref: 69634
  • oxygen tolerance: obligate anaerobe

nutrition type

  • @ref: 69634
  • type: chemoorganoheterotroph

spore formation

  • @ref: 69634
  • type of spore: spore
  • spore formation: no

halophily

@refhalophily levelsaltgrowthtested relationconcentration
69634halophilicNaClpositivegrowth0.5-2.5 %(w/v)
69634NaClpositiveoptimum1.5 %(w/v)

observation

  • @ref: 69634
  • observation: filamentous rods, occasionally forming loops and knots

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6963417632nitrate-electron acceptor
6963416189sulfate-electron acceptor
6963417359sulfite-electron acceptor
6963416094thiosulfate-electron acceptor
6963416449alanine-growth
6963433709amino acid-growth
6963422599arabinose-growth
6963429016arginine-growth
6963417057cellobiose-growth
6963462968cellulose-growth
6963417029chitin-growth
6963416261chitosan-growth
6963423652dextrin-growth
6963428260galactose-growth
6963417754glycerol-growth
6963428087glycogen-growth
69634guar gum-growth
6963427570histidine-growth
69634karaya gum-growth
6963424996lactate-growth
6963417716lactose-growth
696346452lichenin-growth
696346457lignin-growth
69634locust bean gum-growth
6963418257ornithine-growth
6963417309pectin-growth
6963426271proline-growth
6963415361pyruvate-growth
6963426546rhamnose-growth
6963417992sucrose-growth
6963426986threonine-growth
6963416199urea-growth
6963410057xanthan-growth
6963437166xylan-growth
6963418222xylose-growth
6963430089acetate+electron donor
6963418276dihydrogen+electron donor
6963417234glucose+electron donor
69634peptone+electron donor
6963417234glucose+fermentation
6963458187alginate+growth
6963428793beta-D-glucan+growth
6963428757fructose+growth
696345291gelatin+growth
6963417234glucose+growth
69634keratin+growth
696346364laminarin+growth
6963417306maltose+growth
6963437684mannose+growth
69634peptone+growth
6963427941pullulan+growth
6963428017starch+growth
69634tryptone+growth
69634yeast extract+growth
69634yeast extract+required for growth

antibiotic resistance

@refChEBImetaboliteis antibioticis sensitiveis resistant
6963428971ampicillinyesyes
6963417698chloramphenicolyesyes
6963428368novobiocinyesyes
6963428077rifampicinyesyes
6963427902tetracyclineyesyes
6963428001vancomycinyesyes
696346104kanamycinyesyes
696347507neomycinyesyes
696348309polymyxin byesyes
6963417076streptomycinyesyes

metabolite production

  • @ref: 69634
  • Chebi-ID: 30089
  • metabolite: acetate
  • production: yes

fatty acid profile

  • fatty acids

    @reffatty acidpercentage
    69634anteiso-C15:012.1
    69634anteiso-C15:10.35
    69634C14:00.21
    69634C16:00.6
    69634iso-C13:00.61
    69634iso-C14:00.25
    69634iso-C15:081.6
    69634iso-C15:0 3OH2.07
    69634iso-C15:11.35
    69634iso-C16:00.22
    69634iso-C17:1 w8c0.68
  • type of FA analysis: whole cell analysis
  • system: MIS MIDI

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentsampling dateenrichment cultureenrichment culture temperatureisolation dateisolation procedure
66809mixture of iron-contining deposits, shallow hot springKunashir IslandRussiaRUSEurope
69634Shallow hot springKunashir IslandRussiaRUSEurope2014-07-01modified MJ medium602014-10-31anaerobic

Sequence information

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
69634Tenuifilum thalassicum 38H-strGCA_013265555completencbi2590900
69634Whole genome shotgunCP041345ncbi2590900

GC content

  • @ref: 69634
  • GC-content: 38.3
  • method: genome sequence analysis

External links

@ref: 66809

culture collection no.: DSM 100343, VKM B-2964

straininfo link

  • @ref: 112188
  • straininfo: 399977

literature

  • topic: Phylogeny
  • Pubmed-ID: 32847792
  • title: Tenuifilum thalassicum gen. nov., sp. nov., a novel moderate thermophilic anaerobic bacterium from a Kunashir Island shallow hot spring representing a new family Tenuifilaceae fam. nov. in the class Bacteroidia.
  • authors: Podosokorskaya OA, Kochetkova TV, Novikov AA, Toshchakov SV, Elcheninov AG, Kublanov IV
  • journal: Syst Appl Microbiol
  • DOI: 10.1016/j.syapm.2020.126126
  • year: 2020
  • mesh: Anaerobiosis, Bacteroidetes/*classification/genetics/*isolation & purification/physiology, Base Composition, Carbohydrate Metabolism, Carbohydrates, DNA, Bacterial/chemistry/genetics, Fatty Acids/analysis, Fermentation, Genes, Bacterial, Genes, rRNA, Genome, Bacterial, Hot Springs/*microbiology, Hot Temperature, Lipids/analysis, Peptides/metabolism, Phenotype, Phylogeny, Proteins/metabolism, RNA, Ribosomal, 16S/genetics, Russia, Sequence Analysis, DNA
  • topic2: Metabolism

Reference

@idauthorstitledoi/urlcatalogue
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
66809Curators of the DSMZhttps://www.dsmz.de/collection/catalogue/details/culture/DSM-100343Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 100343)
69634Olga A. Podosokorskaya, Tatiana V. Kochetkova, Andrei A. Novikov, Stepan V. Toshchakov, Alexander G. Elcheninov, Ilya V. KublanovTenuifilum thalassicum gen. nov., sp. nov., a novel moderate thermophilic anaerobic bacterium from a Kunashir Island shallow hot spring representing a new family Tenuifilaceae fam. nov. in the class Bacteroidia10.1016/j.syapm.2020.126126
112188Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.StrainInfo: A central database for resolving microbial strain identifiers10.60712/SI-ID399977.1