Strain identifier

BacDive ID: 159885

Type strain: Yes

Species: Brevibacterium hankyongi

Strain Designation: BS05

Strain history: W.-T. Im; Hankyong Natl. Univ., South Korea; BS05.

NCBI tax ID(s): 1007087 (species)

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General

@ref: 66626

BacDive-ID: 159885

keywords: 16S sequence, Bacteria, obligate aerobe, psychrophilic, Gram-positive, rod-shaped

description: Brevibacterium hankyongi BS05 is an obligate aerobe, psychrophilic, Gram-positive bacterium that was isolated from compost made from foodstuff waste.

NCBI tax id

  • NCBI tax id: 1007087
  • Matching level: species

strain history

  • @ref: 67770
  • history: W.-T. Im; Hankyong Natl. Univ., South Korea; BS05.

doi: 10.13145/bacdive159885.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Micrococcales
  • family: Brevibacteriaceae
  • genus: Brevibacterium
  • species: Brevibacterium hankyongi
  • full scientific name: Brevibacterium hankyongi Choi et al. 2018

@ref: 66626

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Micrococcales

family: Brevibacteriaceae

genus: Brevibacterium

species: Brevibacterium hankyongi

strain designation: BS05

type strain: yes

Morphology

cell morphology

  • @ref: 66626
  • gram stain: positive
  • cell length: 1.3-2.8 µm
  • cell width: 0.3-1.0 µm
  • cell shape: rod-shaped
  • motility: no

colony morphology

  • @ref: 66626
  • colony color: milky-white to cream
  • incubation period: 2 days
  • medium used: R2A medium

Culture and growth conditions

culture medium

  • @ref: 66626
  • name: Reasoner's 2A agar (R2A)
  • growth: yes

culture temp

@refgrowthtypetemperaturerange
66626nogrowth15psychrophilic
66626positiveoptimum30mesophilic
66626nogrowth45thermophilic
66626positivegrowth18-42
67770positivegrowth28mesophilic

culture pH

@refabilitytypepH
66626positiveoptimum6.5
66626positivegrowth6.0-7.0

Physiology and metabolism

oxygen tolerance

  • @ref: 66626
  • oxygen tolerance: obligate aerobe

halophily

@refsaltgrowthtested relationconcentrationhalophily level
66626NaClpositivegrowth0-6.0 %(w/v)
66626NaClpositiveoptimum0 %(w/v)non-halophilic

observation

  • @ref: 67770
  • observation: quinones: MK-8(H2), MK-7(H2)

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6662615792malonate-assimilation
6662616024D-mannose-assimilation
6662616411acetic acid-assimilation
66626168082-dehydro-D-gluconate-assimilation
6662616899D-mannitol-assimilation
6662616988D-ribose-assimilation
6662617128adipate-assimilation
6662617203L-proline-assimilation
6662617240itaconate-assimilation
6662617268myo-inositol-assimilation
6662617306maltose-assimilation
6662617634D-glucose-assimilation
6662617814salicin-assimilation
6662617924D-sorbitol-assimilation
6662618287L-fucose-assimilation
6662624265gluconate-assimilation
6662624996lactate-assimilation
6662627689decanoate-assimilation
6662628053melibiose-assimilation
6662630849L-arabinose-assimilation
666269300suberic acid+assimilation
6662615971L-histidine+assimilation
66626161933-hydroxybenzoate+assimilation
66626167244-hydroxybutyrate+assimilation
6662616977L-alanine+assimilation
6662617115L-serine+assimilation
6662617272propionate+assimilation
6662617306maltose+assimilation
6662617992sucrose+assimilation
6662618401phenylacetate+assimilation
6662625115malate+assimilation
6662627689decanoate+assimilation
6662628087glycogen+assimilation
66626370543-hydroxybutyrate+assimilation
6662653258citric acid+assimilation
66626581435-dehydro-D-gluconate+assimilation
6662659640N-acetylglucosamine+assimilation
6662662345L-rhamnose+assimilation
6662617234glucose-builds acid from
6662617632nitrate-reduction

metabolite production

  • @ref: 66626
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

enzymes

@refvalueactivityec
66626acid phosphatase-3.1.3.2
66626alpha-chymotrypsin-3.4.21.1
66626alpha-fucosidase-3.2.1.51
66626alpha-galactosidase-3.2.1.22
66626alpha-glucosidase-3.2.1.20
66626alpha-mannosidase-3.2.1.24
66626arginine dihydrolase-3.5.3.6
66626beta-galactosidase-3.2.1.23
66626beta-glucosidase-3.2.1.21
66626beta-glucuronidase-3.2.1.31
66626esterase Lipase (C 8)-
66626lipase (C 14)-
66626N-acetyl-beta-glucosaminidase-3.2.1.52
66626naphthol-AS-BI-phosphohydrolase-
66626trypsin-3.4.21.4
66626urease-3.5.1.5
66626valine arylamidase-
66626alkaline phosphatase+3.1.3.1
66626catalase+1.11.1.6
66626cystine arylamidase+3.4.11.3
66626cytochrome oxidase+1.9.3.1
66626esterase+
66626leucine arylamidase+3.4.11.1

fatty acid profile

  • fatty acids

    @reffatty acidpercentage
    66626C15:0 iso13
    66626C16:00.6
    66626C17:0 iso2.1
    66626C16:0 iso22.6
    66626C15:0 anteiso27.7
    66626C17:0 anteiso32.4
  • type of FA analysis: whole cell analysis
  • incubation medium: R2A
  • incubation temperature: 30
  • incubation time: 2
  • library/peak naming table: TSBA60
  • system: MIS MIDI
  • method/protocol: Sasser, 1990
  • cutoff value:

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentenrichment cultureenrichment culture durationenrichment culture temperatureisolation procedure
66626compost made from foodstuff wasteJeju IslandRepublic of KoreaKORAsia1/2 R2A5 days301 g of sample was dissolved in 10 ml 0.85 % sterile saline.
67770SeawaterJeju IslandRepublic of KoreaKORAsia

isolation source categories

Cat1Cat2Cat3
#Engineered#Biodegradation#Composting
#Engineered#Food production#Food

taxonmaps

  • @ref: 69479
  • File name: preview.99_167766.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_15817;96_62736;97_81327;98_110097;99_167766&stattab=map
  • Last taxonomy: Brevibacterium hankyongi subclade
  • 16S sequence: JF806518
  • Sequence Identity:
  • Total samples: 314
  • soil counts: 41
  • aquatic counts: 31
  • animal counts: 232
  • plant counts: 10

Sequence information

16S sequences

  • @ref: 66626
  • description: Brevibacterium sp. BS05 16S ribosomal RNA gene, partial sequence
  • accession: JF806518
  • length: 1458
  • database: ena
  • NCBI tax ID: 1007087

GC content

@refGC-contentmethod
6662662.30high performance liquid chromatography (HPLC)
6777062.3high performance liquid chromatography (HPLC)

External links

@ref: 66626

culture collection no.: KACC 18875, LMG 29562, JCM 30949

literature

  • topic: Phylogeny
  • Pubmed-ID: 30063198
  • title: Brevibacterium hankyongi sp. nov., isolated from compost.
  • authors: Choi KD, Siddiqi MZ, Liu Q, Muhammad Shafi S, Durrani Y, Lee SY, Kang MS, Im WT
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijsem.0.002886
  • year: 2018
  • mesh: Bacterial Typing Techniques, Base Composition, Brevibacterium/*classification/genetics/isolation & purification, *Composting, DNA, Bacterial/genetics, Diaminopimelic Acid/chemistry, Fatty Acids/chemistry, Nucleic Acid Hybridization, Peptidoglycan/chemistry, Phosphatidylglycerols/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Republic of Korea, Sequence Analysis, DNA, *Soil Microbiology
  • topic2: Transcriptome

Reference

@idauthorstitledoi/urljournalpubmed
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
66626Kang Duk Choi, Muhammad Zubair Siddiqi, Qingmei Liu, Siddiqi Muhammad Shafi, Yasser Durrani, Soon Yeol Lee, Myung-Suk Kang and Wan Taek ImBrevibacterium hankyongi sp. nov., isolated from compost10.1099/ijsem.0.002886IJSEM 68: 2783-2788 201830063198
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.MicrobeAtlas 1.0 betahttps://microbeatlas.org/