Strain identifier

BacDive ID: 159884

Type strain: Yes

Species: Pseudomonas tianjinensis

Strain Designation: 68

NCBI tax ID(s): 2282554 (species)

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General

@ref: 66568

BacDive-ID: 159884

keywords: 16S sequence, Bacteria, aerobe, psychrophilic, Gram-negative, rod-shaped, colony-forming

description: Pseudomonas tianjinensis 68 is an aerobe, psychrophilic, Gram-negative bacterium that forms circular colonies and was isolated from wastewater from a domestic sewage discharge drain.

NCBI tax id

  • NCBI tax id: 2282554
  • Matching level: species

doi: 10.13145/bacdive159884.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Pseudomonadales
  • family: Pseudomonadaceae
  • genus: Pseudomonas
  • species: Pseudomonas tianjinensis
  • full scientific name: Pseudomonas tianjinensis Chen et al. 2018

@ref: 66568

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Pseudomonadales

family: Pseudomonadaceae

genus: Pseudomonas

species: Pseudomonas tianjinensis

strain designation: 68

type strain: yes

Morphology

cell morphology

  • @ref: 66568
  • gram stain: negative
  • cell length: 1.2-3.2 µm
  • cell width: 0.3-0.4 µm
  • cell shape: rod-shaped

colony morphology

  • @ref: 66568
  • colony color: light yellow
  • colony shape: circular
  • incubation period: 1 day
  • medium used: Trypticase Soy Agar (TSA)

Culture and growth conditions

culture medium

  • @ref: 66568
  • name: tryptic soy agar (TSA)
  • growth: yes

culture temp

@refgrowthtypetemperaturerange
66568nogrowth15psychrophilic
66568nogrowth42thermophilic
66568positivegrowth15-42

culture pH

@refabilitytypepHPH range
66568positiveoptimum8
66568positivegrowth6.0-9.0alkaliphile

Physiology and metabolism

oxygen tolerance

  • @ref: 66568
  • oxygen tolerance: aerobe

spore formation

  • @ref: 66568
  • spore formation: no

halophily

  • @ref: 66568
  • salt: NaCl
  • growth: positive
  • tested relation: growth
  • concentration: 0-4 %(w/v)

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
665686731melezitose-builds acid from
6656812936D-galactose-builds acid from
6656815443inulin-builds acid from
6656815963ribitol-builds acid from
6656816024D-mannose-builds acid from
6656816443D-tagatose-builds acid from
6656816634raffinose-builds acid from
66568168082-dehydro-D-gluconate-builds acid from
6656816813galactitol-builds acid from
6656816988D-ribose-builds acid from
6656817057cellobiose-builds acid from
6656817108D-arabinose-builds acid from
6656817113erythritol-builds acid from
6656817151xylitol-builds acid from
6656817266L-sorbose-builds acid from
6656817268myo-inositol-builds acid from
6656817716lactose-builds acid from
6656817814salicin-builds acid from
6656817992sucrose-builds acid from
6656818287L-fucose-builds acid from
6656818305arbutin-builds acid from
6656822605arabinitol-builds acid from
6656824265gluconate-builds acid from
6656827082trehalose-builds acid from
6656827613amygdalin-builds acid from
6656828017starch-builds acid from
6656828053melibiose-builds acid from
6656828066gentiobiose-builds acid from
6656828087glycogen-builds acid from
6656828847D-fucose-builds acid from
6656829864mannitol-builds acid from
6656830849L-arabinose-builds acid from
6656830911sorbitol-builds acid from
6656832528turanose-builds acid from
6656843943methyl alpha-D-mannoside-builds acid from
66568581435-dehydro-D-gluconate-builds acid from
6656859640N-acetylglucosamine-builds acid from
6656862318D-lyxose-builds acid from
6656862345L-rhamnose-builds acid from
6656865327D-xylose-builds acid from
6656865328L-xylose-builds acid from
6656874863methyl beta-D-xylopyranoside-builds acid from
66568320061methyl alpha-D-glucopyranoside-builds acid from
6656815824D-fructose+builds acid from
6656817306maltose+builds acid from
6656817634D-glucose+builds acid from
665684853esculin+/-builds acid from
6656817754glycerol+/-builds acid from
665686472lincomycin+carbon source
665688391D-gluconate+carbon source
6656812936D-galactose+carbon source
6656813705acetoacetate+carbon source
6656814314D-glucose 6-phosphate+carbon source
6656815588D-malate+carbon source
6656815589L-malate+carbon source
6656815748D-glucuronate+carbon source
6656815824D-fructose+carbon source
6656815895D-galactonic acid lactone+carbon source
6656815971L-histidine+carbon source
6656816024D-mannose+carbon source
6656816411acetic acid+carbon source
6656816467L-arginine+carbon source
6656816523D-serine+carbon source
6656816537galactarate+carbon source
6656816651(S)-lactate+carbon source
6656816977L-alanine+carbon source
6656817115L-serine+carbon source
6656817272propionate+carbon source
6656817306maltose+carbon source
6656817754glycerol+carbon source
6656817925alpha-D-glucose+carbon source
6656818024D-galacturonic acid+carbon source
6656818287L-fucose+carbon source
6656823652dextrin+carbon source
6656826490quinate+carbon source
6656828001vancomycin+carbon source
6656828053melibiose+carbon source
6656828066gentiobiose+carbon source
6656828847D-fucose+carbon source
6656829673rifamycin sv+carbon source
6656829985L-glutamate+carbon source
6656829991L-aspartate+carbon source
6656832323glucuronamide+carbon source
6656832735guanidinium chloride+carbon source
66568370543-hydroxybutyrate+carbon source
6656845735troleandomycin+carbon source
6656848607lithium chloride+carbon source
6656850694minocycline+carbon source
6656851850methyl pyruvate+carbon source
6656853258citric acid+carbon source
6656853423tween 40+carbon source
6656862345L-rhamnose+carbon source
6656871321fusidate+carbon source
6656873706bromosuccinate+carbon source
66568739183-O-methyl-D-glucose+carbon source
6656874611methyl (R)-lactate+carbon source
6656875193tetrazolium violet+carbon source
6656875198tetrazolium blue+carbon source
6656875229sodium bromate+carbon source
6656875248potassium tellurite+carbon source
6656875273niaproof+carbon source
6656878697D-fructose 6-phosphate+carbon source
66568161680aztreonam+carbon source
665681 % sodium lactate+carbon source
66568168102-oxoglutarate+carbon source
6656827897tryptophan-energy source
6656817234glucose-fermentation
6656817268myo-inositol-fermentation
6656817992sucrose-fermentation
6656822599arabinose-fermentation
6656826546rhamnose-fermentation
6656827613amygdalin-fermentation
6656828053melibiose-fermentation
6656829864mannitol-fermentation
6656830911sorbitol-fermentation
6656817234glucose-oxidation
6656817268myo-inositol-oxidation
6656817992sucrose-oxidation
6656822599arabinose-oxidation
6656826546rhamnose-oxidation
6656827613amygdalin-oxidation
6656828053melibiose-oxidation
6656829864mannitol-oxidation
6656830911sorbitol-oxidation

metabolite production

@refChebi-IDmetaboliteproduction
6656815688acetoinno
6656816136hydrogen sulfideno
6656835581indoleno

metabolite tests

@refChebi-IDmetaboliteindole testvoges-proskauer-test
6656835581indole-
6656815688acetoin-

enzymes

@refvalueactivityec
66568alpha-glucosidase-3.2.1.20
66568alpha-mannosidase-3.2.1.24
66568beta-D-fucosidase-3.2.1.38
66568beta-galactosidase-3.2.1.23
66568beta-glucosidase-3.2.1.21
66568chymotrypsin-3.4.4.5
66568cystine arylamidase-3.4.11.3
66568lysine decarboxylase-4.1.1.18
66568N-acetyl-beta-glucosaminidase-3.2.1.52
66568ornithine decarboxylase-4.1.1.17
66568trypsin-3.4.21.4
66568tryptophan deaminase-4.1.99.1
66568urease-3.5.1.5
66568valine arylamidase-
66568acid phosphatase+3.1.3.2
66568alkaline phosphatase+3.1.3.1
66568arginine dihydrolase+3.5.3.6
66568esterase (C 4)+
66568esterase Lipase (C 8)+
66568leucine arylamidase+3.4.11.1
66568naphthol-AS-BI-phosphohydrolase+
66568alpha-galactosidase+/-3.2.1.22
66568beta-galactosidase+/-3.2.1.23
66568beta-glucuronidase+/-3.2.1.31

Isolation, sampling and environmental information

isolation

  • @ref: 66568
  • sample type: wastewater from a domestic sewage discharge drain
  • geographic location: Tianjin
  • country: China
  • origin.country: CHN
  • continent: Asia
  • latitude: 39.05
  • longitude: 117.1
  • enrichment culture: TSB
  • enrichment culture duration: 4 days
  • enrichment culture temperature: 30

isolation source categories

Cat1Cat2Cat3
#Engineered#Waste#Sewage sludge
#Engineered#Waste#Wastewater

taxonmaps

  • @ref: 69479
  • File name: preview.99_6146.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_23;96_61;97_64;98_4558;99_6146&stattab=map
  • Last taxonomy: Pseudomonas
  • 16S sequence: MF083697
  • Sequence Identity:
  • Total samples: 6063
  • soil counts: 1066
  • aquatic counts: 2088
  • animal counts: 1882
  • plant counts: 1027

Sequence information

16S sequences

  • @ref: 66568
  • description: Pseudomonas sp. strain 68 16S ribosomal RNA gene, partial sequence
  • accession: MF083697
  • length: 1498
  • database: ena
  • NCBI tax ID: 2282554

GC content

  • @ref: 66568
  • GC-content: 61.90
  • method: high performance liquid chromatography (HPLC)

External links

@ref: 66568

culture collection no.: CICC 24204, KCTC 52977

literature

  • topic: Phylogeny
  • Pubmed-ID: 30024362
  • title: Pseudomonas tianjinensis sp. nov., isolated from domestic sewage.
  • authors: Chen P, Li S, Li QX
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijsem.0.002799
  • year: 2018
  • mesh: Bacterial Typing Techniques, Base Composition, China, DNA, Bacterial/genetics, Fatty Acids/chemistry, Nucleic Acid Hybridization, Phospholipids/chemistry, *Phylogeny, Pseudomonas/*classification/genetics/isolation & purification, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Sewage/*microbiology, Vitamin K 2/analogs & derivatives/chemistry
  • topic2: Transcriptome

Reference

@idauthorstitledoi/urljournalpubmed
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
66568Peizhen Chen, Shaopeng Li and Qing X. LiPseudomonas tianjinensis sp. nov., isolated from domestic sewage10.1099/ijsem.0.002799IJSEM 68: 2760-2769 201830024362
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.MicrobeAtlas 1.0 betahttps://microbeatlas.org/