Strain identifier

BacDive ID: 159877

Type strain: Yes

Species: Piscinibacter caeni

Strain Designation: MQ-18

Strain history: D.-Z. Chen; Zhejiang Univ. of Technol., China; MQ-18.

NCBI tax ID(s): 1861917 (species)

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General

@ref: 66628

BacDive-ID: 159877

keywords: genome sequence, 16S sequence, Bacteria, aerobe, mesophilic, Gram-negative, motile, rod-shaped

description: Piscinibacter caeni MQ-18 is an aerobe, mesophilic, Gram-negative bacterium that was isolated from activated sludge of a pharmaceutical factory.

NCBI tax id

  • NCBI tax id: 1861917
  • Matching level: species

strain history

  • @ref: 67770
  • history: D.-Z. Chen; Zhejiang Univ. of Technol., China; MQ-18.

doi: 10.13145/bacdive159877.20250331.9.3

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Betaproteobacteria
  • order: Burkholderiales
  • family: Sphaerotilaceae
  • genus: Piscinibacter
  • species: Piscinibacter caeni
  • full scientific name: Piscinibacter caeni Chen et al. 2018

@ref: 66628

domain: Bacteria

phylum: Proteobacteria

class: Betaproteobacteria

order: Burkholderiales

family: Burkholderiales, not assigned to family

genus: Piscinibacter

species: Piscinibacter caeni

strain designation: MQ-18

type strain: yes

Morphology

cell morphology

  • @ref: 66628
  • gram stain: negative
  • cell length: 1.9-2.7 µm
  • cell width: 0.5-0.75 µm
  • cell shape: rod-shaped
  • motility: yes
  • flagellum arrangement: polar

colony morphology

  • @ref: 66628
  • colony color: yellow
  • medium used: R2A medium

Culture and growth conditions

culture medium

  • @ref: 66628
  • name: Reasoner's 2A agar (R2A)
  • growth: yes

culture temp

@refgrowthtypetemperature
66628positiveoptimum30
66628positivegrowth20-40
67770positivegrowth30

culture pH

@refabilitytypepHPH range
66628positiveoptimum7
66628positivegrowth6.0-9.0alkaliphile

Physiology and metabolism

oxygen tolerance

@refoxygen toleranceconfidence
66628aerobe
125439obligate aerobe99.7

spore formation

@refspore formationconfidence
66628no
125439no97.3

halophily

@refsaltgrowthtested relationconcentrationhalophily level
66628NaClpositivegrowth0-0.8 %(w/v)
66628NaClpositiveoptimum0 %non-halophilic

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6662817128adipate-assimilation
6662818401phenylacetate-assimilation
6662824265gluconate-assimilation
6662825115malate-assimilation
6662827689decanoate-assimilation
6662853258sodium citrate-assimilation
6662859640N-acetylglucosamine-assimilation
6662816024D-mannose+assimilation
6662816899D-mannitol+assimilation
6662817306maltose+assimilation
6662817634D-glucose+assimilation
6662830849L-arabinose+/-assimilation
666285291gelatin-carbon source
6662812936D-galactose-carbon source
6662813705acetoacetate-carbon source
6662814314D-glucose 6-phosphate-carbon source
6662815740formate-carbon source
6662815748D-glucuronate-carbon source
6662815971L-histidine-carbon source
6662816467L-arginine-carbon source
6662816523D-serine-carbon source
6662816537galactarate-carbon source
6662816634raffinose-carbon source
6662817115L-serine-carbon source
6662817164stachyose-carbon source
6662817272propionate-carbon source
6662817464L-galactonic acid gamma-lactone-carbon source
6662817596inosine-carbon source
6662817716lactose-carbon source
6662818024D-galacturonic acid-carbon source
66628181014-hydroxyphenylacetic acid-carbon source
6662818183L-pyroglutamic acid-carbon source
6662828053melibiose-carbon source
6662828066gentiobiose-carbon source
6662828800N-acetylgalactosamine-carbon source
6662828847D-fucose-carbon source
6662829990D-aspartate-carbon source
6662829991L-aspartate-carbon source
6662832323glucuronamide-carbon source
6662832735guanidinium chloride-carbon source
6662835418n-acetylneuraminate-carbon source
66628370543-hydroxybutyrate-carbon source
6662845735troleandomycin-carbon source
6662848607lithium chloride-carbon source
6662851850methyl pyruvate-carbon source
6662853258citric acid-carbon source
6662859640N-acetylglucosamine-carbon source
6662863153N-acetyl-D-mannosamine-carbon source
6662864103sodium butyrate-carbon source
6662870744glycine-proline-carbon source
6662871321fusidate-carbon source
66628739183-O-methyl-D-glucose-carbon source
6662875198tetrazolium blue-carbon source
6662875229sodium bromate-carbon source
6662875273niaproof-carbon source
6662878697D-fructose 6-phosphate-carbon source
666286472lincomycin+carbon source
6662815588D-malate+carbon source
6662815589L-malate+carbon source
6662815748D-glucuronate+carbon source
6662815824D-fructose+carbon source
6662816024D-mannose+carbon source
6662816411acetic acid+carbon source
6662816551D-trehalose+carbon source
6662816651(S)-lactate+carbon source
66628167632-oxobutanoate+carbon source
6662816865gamma-aminobutyric acid+carbon source
6662816899D-mannitol+carbon source
6662816977L-alanine+carbon source
6662817057cellobiose+carbon source
6662817268myo-inositol+carbon source
6662817306maltose+carbon source
6662817309pectin+carbon source
6662817754glycerol+carbon source
6662817814salicin+carbon source
6662817924D-sorbitol+carbon source
6662817925alpha-D-glucose+carbon source
6662817992sucrose+carbon source
6662818287L-fucose+carbon source
6662818333D-arabitol+carbon source
6662823652dextrin+carbon source
6662826490quinate+carbon source
6662828001vancomycin+carbon source
6662829673rifamycin sv+carbon source
6662829985L-glutamate+carbon source
6662830612D-glucarate+carbon source
6662832528turanose+carbon source
6662850694minocycline+carbon source
6662853423tween 40+carbon source
6662862345L-rhamnose+carbon source
6662873706bromosuccinate+carbon source
6662874611methyl (R)-lactate+carbon source
6662875193tetrazolium violet+carbon source
6662875248potassium tellurite+carbon source
66628100147nalidixic acid+carbon source
66628161680aztreonam+carbon source
66628320055methyl beta-D-glucopyranoside+carbon source
66628168102-oxoglutarate+carbon source
66628alpha-hydroxybutyrate+carbon source
6662817234glucose-fermentation
6662862968cellulose-hydrolysis
666284853esculin+hydrolysis
666285291gelatin+hydrolysis
6662828017starch+hydrolysis
6662853424tween 20+hydrolysis
6662853426tween 80+hydrolysis
6662817632nitrate+reduction

metabolite production

  • @ref: 66628
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

enzymes

@refvalueactivityec
66628alkaline phosphatase-3.1.3.1
66628alpha-chymotrypsin-3.4.21.1
66628alpha-galactosidase-3.2.1.22
66628alpha-mannosidase-3.2.1.24
66628arginine dihydrolase-3.5.3.6
66628beta-D-fucosidase-3.2.1.38
66628beta-glucuronidase-3.2.1.31
66628cystine arylamidase-3.4.11.3
66628esterase Lipase (C 8)-
66628leucine arylamidase-3.4.11.1
66628lipase (C 14)-
66628N-acetyl-beta-glucosaminidase-3.2.1.52
66628trypsin-3.4.21.4
66628urease-3.5.1.5
66628valine arylamidase-
66628acid phosphatase+3.1.3.2
66628alpha-glucosidase+3.2.1.20
66628beta-galactosidase+3.2.1.23
66628beta-glucosidase+3.2.1.21
66628catalase+1.11.1.6
66628cytochrome oxidase+1.9.3.1
66628esterase (C 4)+
66628naphthol-AS-BI-phosphohydrolase+

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentenrichment cultureenrichment culture compositionenrichment culture durationenrichment culture temperatureisolation procedure
66628activated sludge of a pharmaceutical factoryZhejiangChinaCHNAsiaR2A0.5 g/l proteose peptone, 0.5 g/l casamino acids, 0.5 g/l soluble starch, 0.5 g/l yeast extract, 0.5 g/l dextrose, 0.3 g/l dipotassium phosphate, 0.05 g/l magnesium sulfate heptahydrate, 0.3 g/l sodium pyruvate, 15 g/l agar.4 days301 ml activated sludge was first cultivated in 50 ml distilled water for 3 days. Next, the solutionwas diluted and spread on R2A agar.
67770Activated sludge from a pharmaceutical factory in ZhejiangChinaCHNAsia

isolation source categories

Cat1Cat2Cat3
#Engineered#Waste#Activated sludge
#Engineered#Industrial#Plant (Factory)

Sequence information

16S sequences

  • @ref: 66628
  • description: Piscinibacter caeni strain MQ-18 16S ribosomal RNA gene, partial sequence
  • accession: KX390668
  • length: 1445
  • database: nuccore
  • NCBI tax ID: 1861917

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Piscinibacter caeni MQ-182883124827draftimg1861917
67770Piscinibacter caeni MQ-18GCA_003265685scaffoldncbi1861917

GC content

  • @ref: 66628
  • GC-content: 70.10
  • method: high performance liquid chromatography (HPLC)

Genome-based predictions

predictions

@refmodeltraitdescriptionpredictionconfidence
125439BacteriaNetspore_formationAbility to form endo- or exosporesno97.3
125439BacteriaNetmotilityAbility to perform movementyes71.8
125439BacteriaNetgram_stainReaction to gram-stainingnegative86.4
125439BacteriaNetoxygen_toleranceOxygenic conditions needed for growthobligate aerobe99.7

External links

@ref: 66628

culture collection no.: CCTCC AB 2017223, JCM 32138

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny29957173Piscinibacter caeni sp. nov., isolated from activated sludge.Chen DZ, Yu NN, Chu QY, Chen J, Ye JX, Cheng ZW, Zhang SH, Chen JMInt J Syst Evol Microbiol10.1099/ijsem.0.0028912018Bacteria/genetics, Bacterial Typing Techniques, Base Composition, Burkholderiales/*classification/genetics/isolation & purification, Cadaverine/chemistry, China, DNA, Bacterial/genetics, Drug Industry, Fatty Acids/chemistry, Industrial Waste, Nucleic Acid Hybridization, Phospholipids/chemistry, *Phylogeny, Pigmentation, Putrescine/chemistry, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Sewage/*microbiology, Ubiquinone/chemistryTranscriptome
34320440Interaction of tetrahydrofuran and methyl tert-butyl ether in waste gas treatment by a biotrickling filter bioaugmented with Piscinibacter caeni MQ-18 and Pseudomonas oleovorans DT4.Liu HY, Yang GF, Cheng ZW, Chu QY, Xu YF, Zhang WX, Ye JX, Chen JM, Wang LN, Yang ZY, Tang ZQ, Chen DZChemosphere10.1016/j.chemosphere.2021.1315522021Biodegradation, Environmental, Burkholderiales, Furans, *Methyl Ethers/analysis, *Pseudomonas oleovorans

Reference

@idauthorstitledoi/urljournalpubmed
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
66628Dong-Zhi Chen, Ning-Ning Yu, Qi-Ying Chu, Jing Chen, Jie-Xu Ye, Zhuo-Wei Cheng, Shi-Han Zhang and Jian-Meng ChenPiscinibacter caeni sp. nov., isolated from activated sludge10.1099/ijsem.0.002891IJSEM 68: 2627-2632 201829957173
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
125439Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardydeepG: Deep Learning for Genome Sequence Data. R package version 0.3.1https://github.com/GenomeNet/deepG