Strain identifier

BacDive ID: 159843

Type strain: Yes

Species: Reichenbachiella versicolor

Strain Designation: DC003

NCBI tax ID(s): 1821036 (species)

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General

@ref: 66737

BacDive-ID: 159843

keywords: genome sequence, Bacteria, aerobe, mesophilic, Gram-negative, rod-shaped, colony-forming

description: Reichenbachiella versicolor DC003 is an aerobe, mesophilic, Gram-negative bacterium that forms circular colonies and was isolated from the alga Gracilaria blodgettii of the phylum Rhodophyta collected from the coast of Lingshui county.

NCBI tax id

  • NCBI tax id: 1821036
  • Matching level: species

doi: 10.13145/bacdive159843.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacteroidota
  • domain: Bacteria
  • phylum: Bacteroidota
  • class: Cytophagia
  • order: Cytophagales
  • family: Reichenbachiellaceae
  • genus: Reichenbachiella
  • species: Reichenbachiella versicolor
  • full scientific name: Reichenbachiella versicolor Shi et al. 2018

@ref: 66737

domain: Bacteria

phylum: Bacteroidetes

class: Cytophagia

order: Cytophagales

family: Reichenbachiellaceae

genus: Reichenbachiella

species: Reichenbachiella versicolor

strain designation: DC003

type strain: yes

Morphology

cell morphology

@refgram staincell lengthcell widthcell shapemotility
66737negative3.5-6.0 µm0.2-0.4 µmrod-shaped
66737yes

colony morphology

@refcolony sizecolony shapeincubation periodmedium usedcolony color
667371-2 mmcircular3 daysMarine agar (MA)
667374-5 daysMarine agar (MA)pink
667377 daysMarine agar (MA)dark brown

pigmentation

@refproductionname
66737nocarotenoid
66737noflexirubin

Culture and growth conditions

culture medium

  • @ref: 66737
  • name: Marine agar (MA)
  • growth: yes

culture temp

@refgrowthtypetemperaturerange
66737positiveoptimum28mesophilic
66737positivegrowth10-37

culture pH

@refabilitytypepHPH range
66737positivegrowth6.0-8.5alkaliphile
66737positiveoptimum7.0-7.5

Physiology and metabolism

oxygen tolerance

  • @ref: 66737
  • oxygen tolerance: aerobe

halophily

@refsaltgrowthtested relationconcentration
66737NaClpositivegrowth0.5-7.0 %(w/v)
66737NaClpositiveoptimum2.0-3.0 %(w/v)

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
667376731melezitose-builds acid from
6673712936D-galactose-builds acid from
6673715443inulin-builds acid from
6673715824D-fructose-builds acid from
6673715963ribitol-builds acid from
6673716024D-mannose-builds acid from
6673716634raffinose-builds acid from
6673716813galactitol-builds acid from
6673716899D-mannitol-builds acid from
6673716988D-ribose-builds acid from
6673717057cellobiose-builds acid from
6673717108D-arabinose-builds acid from
6673717113erythritol-builds acid from
6673717151xylitol-builds acid from
6673717266L-sorbose-builds acid from
6673717268myo-inositol-builds acid from
6673717306maltose-builds acid from
6673717634D-glucose-builds acid from
6673717716lactose-builds acid from
6673717754glycerol-builds acid from
6673717814salicin-builds acid from
6673717924D-sorbitol-builds acid from
6673717992sucrose-builds acid from
6673718287L-fucose-builds acid from
6673718305arbutin-builds acid from
6673718333D-arabitol-builds acid from
6673718403L-arabitol-builds acid from
6673727082trehalose-builds acid from
6673727613amygdalin-builds acid from
6673728017starch-builds acid from
6673728053melibiose-builds acid from
6673728066gentiobiose-builds acid from
6673728087glycogen-builds acid from
6673728847D-fucose-builds acid from
6673730849L-arabinose-builds acid from
6673732032potassium gluconate-builds acid from
6673732528turanose-builds acid from
6673743943methyl alpha-D-mannoside-builds acid from
6673759640N-acetylglucosamine-builds acid from
6673762318D-lyxose-builds acid from
6673762345L-rhamnose-builds acid from
6673765327D-xylose-builds acid from
6673765328L-xylose-builds acid from
6673774863methyl beta-D-xylopyranoside-builds acid from
66737320061methyl alpha-D-glucopyranoside-builds acid from
66737potassium 2-dehydro-D-gluconate-builds acid from
6673716443D-tagatose+builds acid from
66737esculin ferric citrate+builds acid from
66737potassium 5-dehydro-D-gluconate+builds acid from
6673717634D-glucose-fermentation
6673753423tween 40-hydrolysis
6673753424tween 20-hydrolysis
6673753425tween 60-hydrolysis
6673753426tween 80-hydrolysis
6673762968cellulose-hydrolysis
667372509agar+hydrolysis
6673728017starch+hydrolysis
6673712936D-galactose+oxidation
6673713705acetoacetate+oxidation
6673715971L-histidine+oxidation
6673716024D-mannose+oxidation
6673717306maltose+oxidation
6673717309pectin+oxidation
6673717925alpha-D-glucose+oxidation
6673732323glucuronamide+oxidation
6673778697D-fructose 6-phosphate+oxidation
6673717632nitrate-reduction

antibiotic resistance

@refChEBImetaboliteis antibioticis resistantresistance conc.is sensitivesensitivity conc.
6673717076streptomycinyesyes10 µg (disc)
6673717833gentamicinyesyes10 µg (disc)
6673728864tobramycinyesyes10 µg (disc)
667376104kanamycinyesyes30 µg (disc)
6673717698chloramphenicolyesyes30 µg (disc)
667377507neomycinyesyes30 µg (disc)
667377731ofloxacinyesyes5 µg (disc)
6673753727sulfamethoxydiazineyesyes5 µg (disc)
6673728077rifampicinyesyes5 µg (disc)
6673729007ceftriaxoneyesyes30 µg (disc)
6673728001vancomycinyesyes30 µg (disc)
6673727902tetracyclineyesyes30 µg (disc)
667373498cefotaxime sodiumyesyes30 µg (disc)
66737100147nalidixic acidyesyes30 µg (disc)
6673731168spiramycin IIyesyes30 µg (disc)
6673717334penicillinyesyes10 µg (disc)
66737100246norfloxacinyesyes10 µg (disc)
667376472lincomycinyesyes2 µg (disc)
6673728971ampicillinyesyes10 µg (disc)
6673748923erythromycinyesyes15 µg (disc)

metabolite production

@refChebi-IDmetaboliteproduction
6673716136hydrogen sulfideno
6673735581indoleno

enzymes

@refvalueactivityec
66737alpha-chymotrypsin-3.4.21.1
66737alpha-fucosidase-3.2.1.51
66737alpha-galactosidase-3.2.1.22
66737alpha-glucosidase-3.2.1.20
66737alpha-mannosidase-3.2.1.24
66737beta-glucosidase-3.2.1.21
66737beta-glucuronidase-3.2.1.31
66737leucine aminopeptidase-3.4.1.1
66737lipase (C 14)-
66737N-acetyl-beta-glucosaminidase-3.2.1.52
66737trypsin-3.4.21.4
66737acid phosphatase+3.1.3.2
66737alkaline phosphatase+3.1.3.1
66737beta-galactosidase+3.2.1.23
66737catalase+1.11.1.6
66737cystine arylamidase+3.4.11.3
66737cytochrome oxidase+1.9.3.1
66737esterase (C 4)+
66737esterase Lipase (C 8)+
66737leucine arylamidase+3.4.11.1
66737naphthol-AS-BI-phosphohydrolase+
66737valine arylamidase+

Isolation, sampling and environmental information

isolation

  • @ref: 66737
  • sample type: the alga Gracilaria blodgettii of the phylum Rhodophyta collected from the coast of Lingshui county
  • host species: Gracilaria blodgettii
  • sampling date: 2015-10
  • geographic location: the coast of Lingshui county
  • country: China
  • origin.country: CHN
  • continent: Asia
  • latitude: 18.4083
  • longitude: 110.062
  • enrichment culture: marine agar 2216
  • enrichment culture duration: 7 days
  • enrichment culture temperature: 25
  • isolation procedure: Strain DC003 was isolated from the alga Gracilaria blodgettii of the phylum Rhodophyta collected from the coast of Lingshui county, Hainan, China in October 2015. The seaweed sample was homogenized with sterile seawater, and the homogenate was spread on marine agar 2216 using a dilution-plating technique. The plates were incubated at 25°C for 7 days. Single colonies from the plates were purified by transferring them onto new MA plates.

isolation source categories

  • Cat1: #Host
  • Cat2: #Algae
  • Cat3: #Red algae

Sequence information

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Reichenbachiella versicolor DC003GCA_003171675contigncbi1821036
66792Reichenbachiella versicolor DC0032882490784draftimg1821036

GC content

  • @ref: 66737
  • GC-content: 37
  • method: high performance liquid chromatography (HPLC)

External links

@ref: 66737

culture collection no.: KCTC 42867, MCCC 1H00130

literature

  • topic: Phylogeny
  • Pubmed-ID: 30231957
  • title: Reichenbachiella versicolor sp. nov., isolated from red alga.
  • authors: Shi MJ, Wang C, Liu ZY, Jiang LX, Du ZJ
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijsem.0.003023
  • year: 2018
  • mesh: Bacterial Typing Techniques, Base Composition, China, Cytophagaceae/*classification/genetics/isolation & purification, DNA, Bacterial/genetics, Fatty Acids/chemistry, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Rhodophyta/*microbiology, Sequence Analysis, DNA, Vitamin K 2/analogs & derivatives/chemistry
  • topic2: Transcriptome

Reference

@idauthorstitledoi/urljournalpubmed
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
66737Ming-Jing Shi, Chong Wang, Zuan-Yan Liu, Lai-Xiang Jiang, Zong-Jun DuReichenbachiella versicolor sp. nov., isolated from red alga10.1099/ijsem.0.003023IJSEM 68: 3523-3527 201830231957
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes