Strain identifier

BacDive ID: 159841

Type strain: Yes

Species: Planococcus halotolerans

NCBI tax ID(s): 2233542 (species)

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General

@ref: 66733

BacDive-ID: 159841

keywords: Bacteria, aerobe, Gram-positive, motile, rod-shaped, colony-forming

description: Planococcus halotolerans CGMCC 1.13628 is an aerobe, Gram-positive, motile bacterium that forms circular colonies and was isolated from saline soil sample.

NCBI tax id

  • NCBI tax id: 2233542
  • Matching level: species

doi: 10.13145/bacdive159841.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Caryophanales
  • family: Caryophanaceae
  • genus: Planococcus
  • species: Planococcus halotolerans
  • full scientific name: Planococcus halotolerans Gan et al. 2018

@ref: 66733

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Caryophanales

family: Caryophanaceae

genus: Planococcus

species: Planococcus halotolerans

type strain: yes

Morphology

cell morphology

  • @ref: 66733
  • gram stain: positive
  • cell length: 0.4-1.6 µm
  • cell width: 0.4-0.6 µm
  • cell shape: rod-shaped
  • motility: yes

colony morphology

  • @ref: 66733
  • colony size: 2-3 µm
  • colony color: orange
  • colony shape: circular
  • incubation period: 2 days
  • medium used: LB agar

Culture and growth conditions

culture medium

  • @ref: 66733
  • name: LB (Luria-Bertani) agar
  • growth: yes

culture temp

@refgrowthtypetemperaturerange
66733positivegrowth0-40
66733positiveoptimum30-35mesophilic

culture pH

@refabilitytypepHPH range
66733positivegrowth6.5-9.0alkaliphile
66733positiveoptimum7.0-7.5

Physiology and metabolism

oxygen tolerance

  • @ref: 66733
  • oxygen tolerance: aerobe

halophily

@refsaltgrowthtested relationconcentrationhalophily level
66733NaClpositivegrowth0-15 %(w/v)
66733NaClpositiveoptimum3 %(w/v)
66733halotolerant

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6673316813galactitol-builds acid from
6673316899D-mannitol-builds acid from
6673317268myo-inositol-builds acid from
6673317754glycerol-builds acid from
6673317924D-sorbitol-builds acid from
6673328017starch-builds acid from
6673330849L-arabinose-builds acid from
6673337684mannose-builds acid from
6673365327D-xylose-builds acid from
6673317057cellobiose+builds acid from
6673317234glucose+builds acid from
6673317306maltose+builds acid from
6673317992sucrose+builds acid from
6673328260galactose+builds acid from
667335291gelatin-hydrolysis
6673316199urea-hydrolysis
6673328017starch-hydrolysis
6673353423tween 40-hydrolysis
6673353426tween 80-hydrolysis
6673353424tween 20+hydrolysis
6673353425tween 60+hydrolysis
66733casein+hydrolysis
6673315895D-galactonic acid lactone-oxidation
66733167244-hydroxybutyrate-oxidation
6673317309pectin-oxidation
6673317924D-sorbitol-oxidation
6673328053melibiose-oxidation
6673351850methyl pyruvate-oxidation
66733645522-hydroxybutyrate-oxidation
6673378697D-fructose 6-phosphate-oxidation
667335291gelatin+oxidation
6673312936D-galactose+oxidation
6673313705acetoacetate+oxidation
6673314314D-glucose 6-phosphate+oxidation
6673315824D-fructose+oxidation
6673316024D-mannose+oxidation
6673316411acetic acid+oxidation
6673316899D-mannitol+oxidation
6673317057cellobiose+oxidation
6673317115L-serine+oxidation
6673317268myo-inositol+oxidation
6673317306maltose+oxidation
6673317754glycerol+oxidation
6673317814salicin+oxidation
6673317925alpha-D-glucose+oxidation
6673317992sucrose+oxidation
6673318183L-pyroglutamic acid+oxidation
6673318333D-arabitol+oxidation
6673323652dextrin+oxidation
6673327082trehalose+oxidation
6673328066gentiobiose+oxidation
6673328120L-fructose+oxidation
6673329985L-glutamate+oxidation
6673329991L-aspartate+oxidation
6673332528turanose+oxidation
6673353423tween 40+oxidation
6673359640N-acetylglucosamine+oxidation
6673363154N-acetyl-beta-D-mannosamine+oxidation
6673317632nitrate-reduction

antibiotic resistance

@refChEBImetaboliteis antibioticis sensitivesensitivity conc.is resistantresistance conc.
667332676amoxicillinyesyes10 µg (disc)
6673317833gentamicinyesyes10 µg (disc)
6673317076streptomycinyesyes10 µg (disc)
6673348923erythromycinyesyes15 µg (disc)
667333534cephalexinyesyes30 µg (disc)
6673317698chloramphenicolyesyes30 µg (disc)
667336104kanamycinyesyes30 µg (disc)
66733100147nalidixic acidyesyes30 µg (disc)
667337507neomycinyesyes30 µg (disc)
667338309polymyxin byesyes30 µg (disc)
6673327902tetracyclineyesyes30 µg (disc)
667337809oxacillinyesyes1 µg (disc)
667333393carbenicillinyesyes100 µg (disc)
6673371415nitrofurantoinyesyes300 µg (disc)
6673317334penicillinyesyes10 Unit (disc)
6673328971ampicillinyesyes10 µg (disc)
667333508ceftazidimeyesyes30 µg (disc)

metabolite production

@refChebi-IDmetaboliteproduction
6673335581indoleyes
6673316136hydrogen sulfideno

enzymes

@refvalueactivityec
66733acid phosphatase-3.1.3.2
66733alpha-fucosidase-3.2.1.51
66733alpha-galactosidase-3.2.1.22
66733alpha-mannosidase-3.2.1.24
66733beta-galactosidase-3.2.1.23
66733beta-glucosidase-3.2.1.21
66733beta-glucuronidase-3.2.1.31
66733lipase (C 14)-
66733N-acetyl-beta-glucosaminidase-3.2.1.52
66733trypsin-3.4.21.4
66733alkaline phosphatase+3.1.3.1
66733alpha-chymotrypsin+3.4.21.1
66733alpha-glucosidase+3.2.1.20
66733catalase+1.11.1.6
66733cystine arylamidase+3.4.11.3
66733cytochrome oxidase+1.9.3.1
66733esterase (C 4)+
66733esterase Lipase (C 8)+
66733leucine arylamidase+3.4.11.1
66733naphthol-AS-BI-phosphohydrolase+
66733valine arylamidase+

fatty acid profile

  • fatty acids

    @reffatty acidpercentage
    66733C14:0 iso10
    66733C16:1 w9c0.8
    66733C18:0 iso0.8
    66733C18:01.8
    66733C16:0 iso10.6
    66733C16:1 w7c alcohol12.9
    66733C18:1 w9c2.7
    66733C17:03.7
    66733C15:0 anteiso39.7
    66733C15:0 iso5.4
    66733C17:0 anteiso5.6
    66733C16:06.3
  • type of FA analysis: whole cell analysis
  • incubation medium: MA
  • agar/liquid: agar
  • incubation temperature: 28
  • incubation time: 18
  • software version: Trace DSQII
  • library/peak naming table: NIST 05
  • system: MIS MIDI
  • method/protocol: Kämpfer & Kroppenstedt 1996
  • cutoff value:

Isolation, sampling and environmental information

isolation

  • @ref: 66733
  • sample type: saline soil sample
  • geographic location: Yadan National Geological Park, Gansu Province, in north-western China
  • country: China
  • origin.country: CHN
  • continent: Asia
  • latitude: 40.4612
  • longitude: 92.9989
  • enrichment culture: modified saline agar medium
  • enrichment culture composition: containing, per litre, 7.5 g casein peptone, 100 g NaCl, 10 g yeast extract, 1.5 ml MS mixture (0.05 g each of betaine, proline, glycine, D-sorbitol and glutamate, added to 1000 ml H2O) and 20 g agar
  • enrichment culture duration: 3 days
  • enrichment culture temperature: 37
  • isolation procedure: was isolated from a saline soil sample collected from Yadan National Geological Park, Gansu Province, in north-western China. To obtain halotolerant and halophilic bacteria, the sample was serially diluted with 0.9 % (w/v) physiological saline solution and then spread on a modified saline agar medium containing, per litre, 7.5 g casein peptone, 100 g NaCl, 10 g yeast extract, 1.5 ml MS mixture (0.05 g each of betaine, proline, glycine, D-sorbitol and glutamate, added to 1000 ml H2O) and 20 g agar (adjusted to pH 8.0 with HCl/NaOH) and incubated at 37°C for 3 days.

isolation source categories

Cat1Cat2Cat3
#Condition#Saline
#Environmental#Terrestrial#Soil

Sequence information

GC content

  • @ref: 66733
  • GC-content: 44.6
  • method: genome sequence analysis

External links

@ref: 66733

culture collection no.: CGMCC 1.13628, KCTC 43001, SCU 63

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny30265231Planococcus halotolerans sp. nov., isolated from a saline soil sample in China.Gan L, Zhang Y, Zhang L, Li X, Wang Z, He L, Li Z, Tian YInt J Syst Evol Microbiol10.1099/ijsem.0.0030192018Bacterial Typing Techniques, Base Composition, China, DNA, Bacterial/genetics, Fatty Acids/chemistry, Nucleic Acid Hybridization, Peptidoglycan/chemistry, Phospholipids/chemistry, *Phylogeny, Pigmentation, Planococcus Bacteria/*classification/genetics/isolation & purification, RNA, Ribosomal, 16S/genetics, *Salinity, Sequence Analysis, DNA, Soil/chemistry, *Soil Microbiology, Vitamin K 2/analogs & derivatives/chemistryTranscriptome
Phylogeny32162498Characteristics of Planococcus antioxidans sp. nov., an antioxidant-producing strain isolated from the desert soil in the Qinghai-Tibetan Plateau.Zhang B, Yang R, Zhang G, Liu Y, Zhang D, Zhang W, Chen T, Liu GMicrobiologyopen10.1002/mbo3.10282020Antioxidants/*metabolism, Bacterial Typing Techniques, Base Composition/genetics, DNA, Bacterial/genetics, Fatty Acids/analysis, Genome, Bacterial/genetics, Planococcus Bacteria/*classification/genetics/isolation & purification/*metabolism, Sequence Analysis, DNA, Soil Microbiology, Tibet, Whole Genome SequencingGenetics
Genetics32681430Genomic insights into the salt tolerance and cold adaptation of Planococcus halotolerans SCU63(T).Gan L, Li X, Tian Y, Peng BArch Microbiol10.1007/s00203-020-01979-92020Acclimatization/*genetics, Cold Temperature, Genomics, Multigene Family/genetics, Phylogeny, Planococcaceae/*genetics/metabolism, Planococcus Bacteria/*genetics/metabolism, Salt Tolerance/*genetics/physiology, Sequence Analysis, DNA, Whole Genome SequencingPhylogeny
Phylogeny33973094Planococcus soli sp. nov., isolated from antarctic soil.Zhang S, Ping W, Xin Y, Xin D, Zhang JAntonie Van Leeuwenhoek10.1007/s10482-021-01581-z2021Antarctic Regions, Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Fatty Acids/analysis, Phylogeny, Planococcaceae, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, *Soil, *Soil MicrobiologyTranscriptome

Reference

@idauthorstitledoi/urljournalpubmed
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
66733Longzhan Gan, Yi Zhang, Lingli Zhang, Xiaoguang Li, Zhikuan Wang, Linling He, Zhenjiang Li, Yongqiang TianPlanococcus halotolerans sp. nov., isolated from a saline soil sample in China10.1099/ijsem.0.003019IJSEM 68: 3500-3505 201830265231