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Strain identifier

BacDive ID: 159831

Type strain: Yes

Species: Flavobacterium effusum

Strain Designation: PSI-22

Strain history: <- Wen-Ming Chen, National Kaohsiung Marine Univ.

NCBI tax ID(s): 1815555 (species)

For citation purpose refer to the digital object identifier (doi) of the current version.
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General

@ref: 66672

BacDive-ID: 159831

keywords: 16S sequence, Bacteria, aerobe, mesophilic, Gram-negative, motile, rod-shaped, colony-forming

description: Flavobacterium effusum PSI-22 is an aerobe, mesophilic, Gram-negative bacterium that forms circular colonies and was isolated from AiLiao River.

NCBI tax id

  • NCBI tax id: 1815555
  • Matching level: species

strain history: <- Wen-Ming Chen, National Kaohsiung Marine Univ.

doi: 10.13145/bacdive159831.20221219.7.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacteroidota
  • domain: Bacteria
  • phylum: Bacteroidota
  • class: Flavobacteriia
  • order: Flavobacteriales
  • family: Flavobacteriaceae
  • genus: Flavobacterium
  • species: Flavobacterium effusum
  • full scientific name: Flavobacterium effusum Chen et al. 2018

@ref: 66672

domain: Bacteria

phylum: Bacteroidetes

class: Flavobacteriia

order: Flavobacteriales

family: Flavobacteriaceae

genus: Flavobacterium

species: Flavobacterium effusum

strain designation: PSI-22

type strain: yes

Morphology

cell morphology

@refgram staincell lengthcell widthcell shapemotilityflagellum arrangement
66672negative2.0-2.2 µm0.4-0.5 µmrod-shapedyes
67771rod-shapedyesgliding
67771negative

colony morphology

  • @ref: 66672
  • colony size: 0.5-1 mm
  • colony color: yellowish orange
  • colony shape: circular
  • incubation period: 2 days
  • medium used: Reasoner's 2A agar (R2A)

pigmentation

  • @ref: 66672
  • production: yes
  • name: Flexirubin-type pigments

Culture and growth conditions

culture medium

@refnamegrowth
66672LB (Luria-Bertani) agaryes
66672Nutrient agar (NA)yes
66672Reasoner's 2A agar (R2A)yes
66672trypticase soy agaryes

culture temp

@refgrowthtypetemperaturerange
66672positiveoptimum30mesophilic
66672positivegrowth15-30
67771positivegrowth25mesophilic

culture pH

@refabilitytypepHPH range
66672positiveoptimum6
66672positivegrowth5-9alkaliphile

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
66672aerobe
67771aerobe

halophily

@refsaltgrowthtested relationconcentration
66672NaClpositivegrowth0-2 %
66672NaClpositiveoptimum0.5 %

observation

  • @ref: 67771
  • observation: quinones: MK-6

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6667218401phenylacetate-assimilation
6667227689decanoate-assimilation
6667217128adipate+assimilation
6667217234glucose+assimilation
6667217306maltose+assimilation
6667222599arabinose+assimilation
6667224265gluconate+assimilation
6667225115malate+assimilation
6667229864mannitol+assimilation
6667237684mannose+assimilation
6667253258citric acid+assimilation
6667259640N-acetylglucosamine+assimilation
6667217234glucose-builds acid from
6667215963ribitol-growth
6667216634raffinose-growth
6667216899D-mannitol-growth
6667217924D-sorbitol-growth
6667217992sucrose-growth
6667253423tween 40-growth
6667253424tween 20-growth
6667253425tween 60-growth
6667253426tween 80-growth
6667212936D-galactose+growth
6667215824D-fructose+growth
6667216024D-mannose+growth
6667217057cellobiose+growth
6667217306maltose+growth
6667217634D-glucose+growth
6667223652dextrin+growth
6667216991dna-hydrolysis
6667253424tween 20-hydrolysis
66672corn oil-hydrolysis
666724853esculin+hydrolysis
666725291gelatin+hydrolysis
6667217029chitin+hydrolysis
6667228017starch+hydrolysis
6667253423tween 40+hydrolysis
6667253425tween 60+hydrolysis
6667253426tween 80+hydrolysis
6667261995lecithin+hydrolysis
6667285146carboxymethylcellulose+hydrolysis
66672casein+hydrolysis
6667217632nitrate-reduction

antibiotic resistance

@refChEBImetaboliteis antibioticis resistantresistance conc.is sensitivesensitivity conc.group ID
6667217833gentamicinyesyes10 µg (disc)
666726104kanamycinyesyes30 µg (disc)
6667217698chloramphenicolyesyes30 µg (disc)
66672100147nalidixic acidyesyes30 µg (disc)
6667228368novobiocinyesyes30 µg (disc)
6667228077rifampicinyesyes5 µg (disc)
6667217076streptomycinyesyes10 µg (disc)
6667227902tetracyclineyesyes30 µg (disc)
6667245924trimethoprimyesyes1.25 µg (disc)26
666729332sulfamethoxazoleyesyes23.75 µg (disc)26
6667218208penicillin gyesyes10 Unit (disc)

metabolite production

  • @ref: 66672
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

enzymes

@refvalueactivityec
66672alpha-fucosidase-3.2.1.51
66672arginine dihydrolase-3.5.3.6
66672lipase (C 14)-
66672trypsin-3.4.21.4
66672urease-3.5.1.5
66672acid phosphatase+3.1.3.2
66672alkaline phosphatase+3.1.3.1
66672alpha-chymotrypsin+3.4.21.1
66672alpha-galactosidase+3.2.1.22
66672alpha-glucosidase+3.2.1.20
66672alpha-mannosidase+3.2.1.24
66672beta-galactosidase+3.2.1.23
66672beta-glucosidase+3.2.1.21
66672beta-glucuronidase+3.2.1.31
66672catalase+1.11.1.6
66672cystine arylamidase+3.4.11.3
66672cytochrome oxidase+1.9.3.1
66672esterase (C 4)+
66672esterase Lipase (C 8)+
66672leucine arylamidase+3.4.11.1
66672N-acetyl-beta-glucosaminidase+3.2.1.52
66672naphthol-AS-BI-phosphohydrolase+
66672valine arylamidase+

fatty acid profile

  • fatty acids

    @reffatty acidpercentage
    66672C14:01.1
    66672C17:0 3OH1.1
    66672C18:1 w9c1.1
    66672C16:0 iso1.2
    66672C15:0 3OH1.5
    66672C16:0 iso 3OH1.5
    66672C17:1 w8c1.6
    66672C18:01.6
    66672C16:1 w6c / C16:1 w7c15.4
    66672anteiso-C15:02.4
    66672C15:0 iso21.7
    66672C16:0 3OH3.5
    66672C17:1 iso w9c / 10-methyl C16:04.2
    66672C15:1 iso4.3
    66672C17:1 w6c4.4
    66672C15:1 w6c5.4
    66672C15:0 iso 3OH6.8
    66672C16:08.3
    66672C17:0 iso 3OH9.8
  • type of FA analysis: whole cell analysis
  • incubation medium: R2A
  • agar/liquid: agar
  • incubation temperature: 30
  • incubation time: 2
  • software version: Sherlock 6.0
  • library/peak naming table: RTSBA6
  • system: MIS MIDI
  • instrument: Hewlett-Packard 5890 Series II
  • cutoff value:

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentlatitudelongitudeenrichment cultureenrichment culture durationenrichment culture temperatureisolation procedure
66672AiLiao RiverAiLiao River of the Castle Peak Village in the vicinity of Pingtung CountyTaiwanTWNAsia22.7508120.605Reasoner's 2A agar3 days25producing yellowish orange colonies, was isolated and selected for detailed tax- onomy analyses. Strain PSI-22 Twas isolated on Reasoner's 2A agar after incubation at 25°C for 3 days, sub-cultured under the same conditions.
67771From water of the AiLiao River of the Castle Peak Village in the vicinity of Pingtung CountyTaiwanTWNAsia22.7508120.605

isolation source categories

  • Cat1: #Environmental
  • Cat2: #Aquatic
  • Cat3: #River (Creek)

Sequence information

16S sequences

  • @ref: 67771
  • description: Flavobacterium sp. PSI-22 partial 16S rRNA gene, strain PSI-22
  • accession: LT223121
  • length: 1439
  • database: ena
  • NCBI tax ID: 1815555

GC content

@refGC-contentmethod
6667241.4high performance liquid chromatography (HPLC)
6777141.4

External links

@ref: 66672

culture collection no.: BCRC 80973, LMG 29553, KCTC 52233

literature

  • topic: Phylogeny
  • Pubmed-ID: 30129917
  • title: Flavobacterium effusum sp. nov., isolated from a freshwater river.
  • authors: Chen WM, Su CL, Kwon SW, Sheu SY
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijsem.0.002944
  • year: 2018
  • mesh: Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Fatty Acids/chemistry, Flavobacterium/*classification/genetics/isolation & purification, Nucleic Acid Hybridization, Phospholipids/chemistry, *Phylogeny, Polyamines/chemistry, RNA, Ribosomal, 16S/genetics, Rivers/*microbiology, Sequence Analysis, DNA, Taiwan, Vitamin K 2/chemistry
  • topic2: Transcriptome

Reference

@idauthorstitledoi/urljournalpubmed
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
66672Wen-Ming Chen, Ching-Ling Su, Soon-Wo Kwon, Shih-Yi SheuFlavobacterium effusum sp. nov., isolated from a freshwater river10.1099/ijsem.0.002944IJSEM 68: 3111-3117 201830129917
67771Curators of the KCTChttps://kctc.kribb.re.kr/En/Kctc