Strain identifier
BacDive ID: 159831
Type strain:
Species: Flavobacterium effusum
Strain Designation: PSI-22
Strain history: <- Wen-Ming Chen, National Kaohsiung Marine Univ.
NCBI tax ID(s): 1815555 (species)
General
@ref: 66672
BacDive-ID: 159831
keywords: 16S sequence, Bacteria, aerobe, mesophilic, Gram-negative, motile, rod-shaped, colony-forming
description: Flavobacterium effusum PSI-22 is an aerobe, mesophilic, Gram-negative bacterium that forms circular colonies and was isolated from AiLiao River.
NCBI tax id
- NCBI tax id: 1815555
- Matching level: species
strain history
- @ref: 67771
- history: <- Wen-Ming Chen, National Kaohsiung Marine Univ.
doi: 10.13145/bacdive159831.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/bacteroidota
- domain: Bacteria
- phylum: Bacteroidota
- class: Flavobacteriia
- order: Flavobacteriales
- family: Flavobacteriaceae
- genus: Flavobacterium
- species: Flavobacterium effusum
- full scientific name: Flavobacterium effusum Chen et al. 2018
@ref: 66672
domain: Bacteria
phylum: Bacteroidetes
class: Flavobacteriia
order: Flavobacteriales
family: Flavobacteriaceae
genus: Flavobacterium
species: Flavobacterium effusum
strain designation: PSI-22
type strain: yes
Morphology
cell morphology
@ref | gram stain | cell length | cell width | cell shape | motility | flagellum arrangement |
---|---|---|---|---|---|---|
66672 | negative | 2.0-2.2 µm | 0.4-0.5 µm | rod-shaped | yes | |
67771 | rod-shaped | yes | gliding | |||
67771 | negative |
colony morphology
- @ref: 66672
- colony size: 0.5-1 mm
- colony color: yellowish orange
- colony shape: circular
- incubation period: 2 days
- medium used: Reasoner's 2A agar (R2A)
pigmentation
- @ref: 66672
- production: yes
- name: Flexirubin-type pigments
Culture and growth conditions
culture medium
@ref | name | growth |
---|---|---|
66672 | LB (Luria-Bertani) agar | yes |
66672 | Nutrient agar (NA) | yes |
66672 | Reasoner's 2A agar (R2A) | yes |
66672 | trypticase soy agar | yes |
culture temp
@ref | growth | type | temperature | range |
---|---|---|---|---|
66672 | positive | optimum | 30 | mesophilic |
66672 | positive | growth | 15-30 | |
67771 | positive | growth | 25 | mesophilic |
culture pH
@ref | ability | type | pH | PH range |
---|---|---|---|---|
66672 | positive | optimum | 6 | |
66672 | positive | growth | 5-9 | alkaliphile |
Physiology and metabolism
oxygen tolerance
@ref | oxygen tolerance |
---|---|
66672 | aerobe |
67771 | aerobe |
halophily
@ref | salt | growth | tested relation | concentration |
---|---|---|---|---|
66672 | NaCl | positive | growth | 0-2 % |
66672 | NaCl | positive | optimum | 0.5 % |
observation
- @ref: 67771
- observation: quinones: MK-6
metabolite utilization
@ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
---|---|---|---|---|
66672 | 18401 | phenylacetate | - | assimilation |
66672 | 27689 | decanoate | - | assimilation |
66672 | 17128 | adipate | + | assimilation |
66672 | 17234 | glucose | + | assimilation |
66672 | 17306 | maltose | + | assimilation |
66672 | 22599 | arabinose | + | assimilation |
66672 | 24265 | gluconate | + | assimilation |
66672 | 25115 | malate | + | assimilation |
66672 | 29864 | mannitol | + | assimilation |
66672 | 37684 | mannose | + | assimilation |
66672 | 53258 | citric acid | + | assimilation |
66672 | 59640 | N-acetylglucosamine | + | assimilation |
66672 | 17234 | glucose | - | builds acid from |
66672 | 15963 | ribitol | - | growth |
66672 | 16634 | raffinose | - | growth |
66672 | 16899 | D-mannitol | - | growth |
66672 | 17924 | D-sorbitol | - | growth |
66672 | 17992 | sucrose | - | growth |
66672 | 53423 | tween 40 | - | growth |
66672 | 53424 | tween 20 | - | growth |
66672 | 53425 | tween 60 | - | growth |
66672 | 53426 | tween 80 | - | growth |
66672 | 12936 | D-galactose | + | growth |
66672 | 15824 | D-fructose | + | growth |
66672 | 16024 | D-mannose | + | growth |
66672 | 17057 | cellobiose | + | growth |
66672 | 17306 | maltose | + | growth |
66672 | 17634 | D-glucose | + | growth |
66672 | 23652 | dextrin | + | growth |
66672 | 16991 | dna | - | hydrolysis |
66672 | 53424 | tween 20 | - | hydrolysis |
66672 | corn oil | - | hydrolysis | |
66672 | 4853 | esculin | + | hydrolysis |
66672 | 5291 | gelatin | + | hydrolysis |
66672 | 17029 | chitin | + | hydrolysis |
66672 | 28017 | starch | + | hydrolysis |
66672 | 53423 | tween 40 | + | hydrolysis |
66672 | 53425 | tween 60 | + | hydrolysis |
66672 | 53426 | tween 80 | + | hydrolysis |
66672 | 61995 | lecithin | + | hydrolysis |
66672 | 85146 | carboxymethylcellulose | + | hydrolysis |
66672 | casein | + | hydrolysis | |
66672 | 17632 | nitrate | - | reduction |
antibiotic resistance
@ref | ChEBI | metabolite | is antibiotic | is resistant | resistance conc. | is sensitive | sensitivity conc. | group ID |
---|---|---|---|---|---|---|---|---|
66672 | 17833 | gentamicin | yes | yes | 10 µg (disc) | |||
66672 | 6104 | kanamycin | yes | yes | 30 µg (disc) | |||
66672 | 17698 | chloramphenicol | yes | yes | 30 µg (disc) | |||
66672 | 100147 | nalidixic acid | yes | yes | 30 µg (disc) | |||
66672 | 28368 | novobiocin | yes | yes | 30 µg (disc) | |||
66672 | 28077 | rifampicin | yes | yes | 5 µg (disc) | |||
66672 | 17076 | streptomycin | yes | yes | 10 µg (disc) | |||
66672 | 27902 | tetracycline | yes | yes | 30 µg (disc) | |||
66672 | 45924 | trimethoprim | yes | yes | 1.25 µg (disc) | 26 | ||
66672 | 9332 | sulfamethoxazole | yes | yes | 23.75 µg (disc) | 26 | ||
66672 | 18208 | penicillin g | yes | yes | 10 Unit (disc) |
metabolite production
- @ref: 66672
- Chebi-ID: 35581
- metabolite: indole
- production: no
enzymes
@ref | value | activity | ec |
---|---|---|---|
66672 | alpha-fucosidase | - | 3.2.1.51 |
66672 | arginine dihydrolase | - | 3.5.3.6 |
66672 | lipase (C 14) | - | |
66672 | trypsin | - | 3.4.21.4 |
66672 | urease | - | 3.5.1.5 |
66672 | acid phosphatase | + | 3.1.3.2 |
66672 | alkaline phosphatase | + | 3.1.3.1 |
66672 | alpha-chymotrypsin | + | 3.4.21.1 |
66672 | alpha-galactosidase | + | 3.2.1.22 |
66672 | alpha-glucosidase | + | 3.2.1.20 |
66672 | alpha-mannosidase | + | 3.2.1.24 |
66672 | beta-galactosidase | + | 3.2.1.23 |
66672 | beta-glucosidase | + | 3.2.1.21 |
66672 | beta-glucuronidase | + | 3.2.1.31 |
66672 | catalase | + | 1.11.1.6 |
66672 | cystine arylamidase | + | 3.4.11.3 |
66672 | cytochrome oxidase | + | 1.9.3.1 |
66672 | esterase (C 4) | + | |
66672 | esterase Lipase (C 8) | + | |
66672 | leucine arylamidase | + | 3.4.11.1 |
66672 | N-acetyl-beta-glucosaminidase | + | 3.2.1.52 |
66672 | naphthol-AS-BI-phosphohydrolase | + | |
66672 | valine arylamidase | + |
fatty acid profile
fatty acids
@ref fatty acid percentage 66672 C14:0 1.1 66672 C17:0 3OH 1.1 66672 C18:1 w9c 1.1 66672 C16:0 iso 1.2 66672 C15:0 3OH 1.5 66672 C16:0 iso 3OH 1.5 66672 C17:1 w8c 1.6 66672 C18:0 1.6 66672 C16:1 w6c / C16:1 w7c 15.4 66672 anteiso-C15:0 2.4 66672 C15:0 iso 21.7 66672 C16:0 3OH 3.5 66672 C17:1 iso w9c / 10-methyl C16:0 4.2 66672 C15:1 iso 4.3 66672 C17:1 w6c 4.4 66672 C15:1 w6c 5.4 66672 C15:0 iso 3OH 6.8 66672 C16:0 8.3 66672 C17:0 iso 3OH 9.8 - type of FA analysis: whole cell analysis
- incubation medium: R2A
- agar/liquid: agar
- incubation temperature: 30
- incubation time: 2
- software version: Sherlock 6.0
- library/peak naming table: RTSBA6
- system: MIS MIDI
- instrument: Hewlett-Packard 5890 Series II
- cutoff value:
Isolation, sampling and environmental information
isolation
@ref | sample type | geographic location | country | origin.country | continent | latitude | longitude | enrichment culture | enrichment culture duration | enrichment culture temperature | isolation procedure |
---|---|---|---|---|---|---|---|---|---|---|---|
66672 | AiLiao River | AiLiao River of the Castle Peak Village in the vicinity of Pingtung County | Taiwan | TWN | Asia | 22.7508 | 120.605 | Reasoner's 2A agar | 3 days | 25 | producing yellowish orange colonies, was isolated and selected for detailed tax- onomy analyses. Strain PSI-22 Twas isolated on Reasoner's 2A agar after incubation at 25°C for 3 days, sub-cultured under the same conditions. |
67771 | From water of the AiLiao River of the Castle Peak Village in the vicinity of Pingtung County | Taiwan | TWN | Asia | 22.7508 | 120.605 |
isolation source categories
- Cat1: #Environmental
- Cat2: #Aquatic
- Cat3: #River (Creek)
Sequence information
16S sequences
- @ref: 67771
- description: Flavobacterium sp. PSI-22 partial 16S rRNA gene, strain PSI-22
- accession: LT223121
- length: 1439
- database: ena
- NCBI tax ID: 1815555
GC content
@ref | GC-content | method |
---|---|---|
66672 | 41.4 | high performance liquid chromatography (HPLC) |
67771 | 41.4 |
External links
@ref: 66672
culture collection no.: BCRC 80973, LMG 29553, KCTC 52233
literature
- topic: Phylogeny
- Pubmed-ID: 30129917
- title: Flavobacterium effusum sp. nov., isolated from a freshwater river.
- authors: Chen WM, Su CL, Kwon SW, Sheu SY
- journal: Int J Syst Evol Microbiol
- DOI: 10.1099/ijsem.0.002944
- year: 2018
- mesh: Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Fatty Acids/chemistry, Flavobacterium/*classification/genetics/isolation & purification, Nucleic Acid Hybridization, Phospholipids/chemistry, *Phylogeny, Polyamines/chemistry, RNA, Ribosomal, 16S/genetics, Rivers/*microbiology, Sequence Analysis, DNA, Taiwan, Vitamin K 2/chemistry
- topic2: Transcriptome
Reference
@id | authors | title | doi/url | journal | pubmed |
---|---|---|---|---|---|
20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | 10.1099/ijsem.0.004332 | ||
66672 | Wen-Ming Chen, Ching-Ling Su, Soon-Wo Kwon, Shih-Yi Sheu | Flavobacterium effusum sp. nov., isolated from a freshwater river | 10.1099/ijsem.0.002944 | IJSEM 68: 3111-3117 2018 | 30129917 |
67771 | Curators of the KCTC | https://kctc.kribb.re.kr/En/Kctc |