Strain identifier

BacDive ID: 159818

Type strain: Yes

Species: Halomonas litopenaei

Strain history: <- Wen-Jun Li, School of Life Sciences, Sun Yat-Sen Univ., China

NCBI tax ID(s): 2109328 (species)

« back to strain detail view
For citation purpose refer to the digital object identifier (doi) of the current version.
Archive
version 8.1 (current version):
version 8:
version 7.1:
version 7:
version 6:
version 5:
version 8.1 (current version)

General

@ref: 66787

BacDive-ID: 159818

keywords: 16S sequence, Bacteria, aerobe, mesophilic, Gram-negative, motile, rod-shaped, colony-forming

description: Halomonas litopenaei NBRC 111829 is an aerobe, mesophilic, Gram-negative bacterium that forms circular colonies and was isolated from water of larviculture.

NCBI tax id

  • NCBI tax id: 2109328
  • Matching level: species

strain history

  • @ref: 67771
  • history: <- Wen-Jun Li, School of Life Sciences, Sun Yat-Sen Univ., China

doi: 10.13145/bacdive159818.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Oceanospirillales
  • family: Halomonadaceae
  • genus: Halomonas
  • species: Halomonas litopenaei
  • full scientific name: Halomonas litopenaei Xue et al. 2018

@ref: 66787

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Oceanospirillales

family: Halomonadaceae

genus: Halomonas

species: Halomonas litopenaei

type strain: yes

Morphology

cell morphology

  • @ref: 66787
  • gram stain: negative
  • cell length: 1.1-1.7 µm
  • cell width: 0.4-0.7 µm
  • cell shape: rod-shaped
  • motility: yes
  • flagellum arrangement: subpolar

colony morphology

  • @ref: 66787
  • colony size: 2-4 mm
  • colony color: light-white
  • colony shape: circular
  • incubation period: 28 days
  • medium used: Marine agar (MA)

Culture and growth conditions

culture medium

@refnamegrowth
66787cetrimide agarno
66787MacConkeyno
66787Marine agar (MA)yes

culture temp

@refgrowthtypetemperaturerange
66787positiveoptimum28-30mesophilic
66787positivegrowth4-45
67771positivegrowth30mesophilic

culture pH

@refabilitytypepHPH range
66787positivegrowth6-9alkaliphile
66787positiveoptimum7.5-8

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
66787aerobe
67771aerobe

halophily

@refsaltgrowthtested relationconcentration
66787NaClpositivegrowth0.5-22 %(w/v)
66787NaClpositiveoptimum3-6 %(w/v)

observation

@refobservation
66787The major respiratory ubiquinone is Q-9.
66787The major polar lipids are diphosphatidylglycerol, phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol, four unidentified phospholipids and three unidentified lipids.

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6678717306maltose-builds acid from
6678717992sucrose-builds acid from
6678727082trehalose-builds acid from
6678717234glucose+builds acid from
6678717268myo-inositol+builds acid from
6678717716lactose+builds acid from
6678722599arabinose+builds acid from
6678726546rhamnose+builds acid from
6678728260galactose+builds acid from
6678728757fructose+builds acid from
6678729864mannitol+builds acid from
6678730911sorbitol+builds acid from
6678737684mannose+builds acid from
6678717234glucose-fermentation
667874853esculin-hydrolysis
6678717203L-proline-hydrolysis
6678728017starch-hydrolysis
6678753424tween 20-hydrolysis
6678753426tween 80-hydrolysis
6678761995lecithin-hydrolysis
66787606565hippurate-hydrolysis
66787casein-hydrolysis
667875291gelatin+hydrolysis
6678716199urea+hydrolysis
6678716414L-valine-nitrogen source
6678716643L-methionine-nitrogen source
6678729990D-aspartate-nitrogen source
6678715971L-histidine+nitrogen source
6678716977L-alanine+nitrogen source
6678717115L-serine+nitrogen source
6678718019L-lysine+nitrogen source
6678724265gluconate+oxidation

metabolite production

@refChebi-IDmetaboliteproduction
6678716136hydrogen sulfideyes
6678715688acetoinno

metabolite tests

@refChebi-IDmetabolitemethylred-testvoges-proskauer-test
6678717234glucose-
6678715688acetoin-

enzymes

@refvalueactivityec
66787phenylalanine deaminase-4.3.1.5
66787catalase+1.11.1.6
66787cytochrome oxidase+1.9.3.1
66787nitrate reductase+1.7.99.4

fatty acid profile

  • fatty acids

    @reffatty acidpercentage
    66787C16:0 3OH0.2
    66787C17:00.2
    66787C18:00.2
    66787C14:00.4
    66787C17:0 cyclo0.8
    66787C19:0 cyclo w8c0.9
    66787C16:018.7
    66787C10:03.3
    66787C12:04.2
    66787C12:0 3OH7.5
  • type of FA analysis: whole cell analysis
  • incubation medium: Marine agar
  • incubation temperature: 28
  • incubation time: 2
  • software version: Sherlock 6.1
  • library/peak naming table: TSBA6
  • system: MIS MIDI

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentlatitudelongitudeenrichment cultureenrichment culture durationenrichment culture temperatureisolation procedure
66787water of larvicultureDonghai Island, Guangdong provinceChinaCHNAsia20.9357110.523marine agar 2216 medium (MA)5 days28The isolation procedure included serial dilution plating on marine agar 2216 medium (MA)
67771From shrimp larviculture waterGuangdong provinceChinaCHNAsia

isolation source categories

  • Cat1: #Environmental
  • Cat2: #Aquatic

taxonmaps

  • @ref: 69479
  • File name: preview.99_3193.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_48;96_430;97_996;98_2449;99_3193&stattab=map
  • Last taxonomy: Halomonas
  • 16S sequence: KP301091
  • Sequence Identity:
  • Total samples: 6315
  • soil counts: 356
  • aquatic counts: 3926
  • animal counts: 1902
  • plant counts: 131

Sequence information

16S sequences

  • @ref: 66787
  • description: Halomonas sp. ZJ2214 16S ribosomal RNA gene, partial sequence
  • accession: KP301091
  • length: 1499
  • database: ena
  • NCBI tax ID: 1612181

GC content

  • @ref: 66787
  • GC-content: 64.1
  • method: genome sequence analysis

External links

@ref: 66787

culture collection no.: NBRC 111829, KCTC 42974, SYSU ZJ2214

literature

  • topic: Phylogeny
  • Pubmed-ID: 30372409
  • title: Halomonas litopenaei sp. nov., a moderately halophilic, exopolysaccharide-producing bacterium isolated from a shrimp hatchery.
  • authors: Xue M, Wen CQ, Liu L, Fang BZ, Salam N, Huang XM, Liu YF, Xiao M, Li WJ
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijsem.0.003090
  • year: 2018
  • mesh: Animals, *Aquaculture, Bacterial Typing Techniques, Base Composition, China, DNA, Bacterial/genetics, Decapoda, Fatty Acids/chemistry, Halomonas/*classification/genetics/isolation & purification, Nucleic Acid Hybridization, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Seawater/*microbiology, Sequence Analysis, DNA, Ubiquinone/chemistry
  • topic2: Transcriptome

Reference

@idauthorstitledoi/urljournalpubmed
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
66787Ming Xue, Chong-Qing Wen, Lan Liu, Bao-Zhu Fang, Nimaichand Salam, Xue-Min Huang, Yang-Feng Liu,Min Xiao and Wen-Jun LiHalomonas litopenaei sp. nov., a moderately halophilic, exopolysaccharide-producing bacterium isolated from a shrimp hatchery10.1099/ijsem.0.003090IJSEM 68: 3914-3921 201830372409
67771Curators of the KCTChttps://kctc.kribb.re.kr/En/Kctc
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.MicrobeAtlas 1.0 betahttps://microbeatlas.org/