Strain identifier

BacDive ID: 159811

Type strain: Yes

Species: Leucothrix arctica

NCBI tax ID(s): 1481894 (species)

For citation purpose refer to the digital object identifier (doi) of the current version.
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General

@ref: 66775

BacDive-ID: 159811

keywords: 16S sequence, Bacteria, aerobe, psychrophilic, Gram-negative, rod-shaped, colony-forming

description: Leucothrix arctica KACC 18010 is an aerobe, psychrophilic, Gram-negative bacterium that forms circular colonies and was isolated from surface seawater sample of the Arctic Circle.

NCBI tax id

  • NCBI tax id: 1481894
  • Matching level: species

doi: 10.13145/bacdive159811.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Beggiatoales
  • family: Leucotrichaceae
  • genus: Leucothrix
  • species: Leucothrix arctica
  • full scientific name: Leucothrix arctica Baek et al. 2018

@ref: 66775

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Thiotrichales

family: Thiotrichaceae

genus: Leucothrix

species: Leucothrix arctica

type strain: yes

Morphology

cell morphology

  • @ref: 66775
  • gram stain: negative
  • cell length: 1.1-2.0 µm
  • cell width: 0.5-0.6 µm
  • cell shape: rod-shaped
  • motility: no

colony morphology

  • @ref: 66775
  • colony size: 1-2 mm
  • colony color: yellow
  • colony shape: circular
  • incubation period: 5 days
  • medium used: Marine agar (MA)

Culture and growth conditions

culture medium

  • @ref: 66775
  • name: Marine agar (MA)
  • growth: yes

culture temp

@refgrowthtypetemperaturerange
66775positiveoptimum15psychrophilic
66775positivegrowth4-37

culture pH

@refabilitytypepHPH range
66775positivegrowth5-10alkaliphile
66775positiveoptimum7.5-8.5

Physiology and metabolism

oxygen tolerance

  • @ref: 66775
  • oxygen tolerance: aerobe

halophily

@refsaltgrowthtested relationconcentration
66775NaClpositivegrowth0.5-10 %(w/v)
66775NaClpositiveoptimum2.0-2.5 %(w/v)

observation

@refobservation
66775The respiratory quinone detected in cells is Q-8.
66775Major polar lipids are diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, an unidentified aminophospholipid, two unidentified phospholipids and an unidentified polar lipid.

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
667756731melezitose-assimilation
6677512936D-galactose-assimilation
6677515443inulin-assimilation
6677515963ribitol-assimilation
6677516024D-mannose-assimilation
6677516443D-tagatose-assimilation
6677516634raffinose-assimilation
6677516813galactitol-assimilation
6677516988D-ribose-assimilation
6677517057cellobiose-assimilation
6677517113erythritol-assimilation
6677517151xylitol-assimilation
6677517234glucose-assimilation
6677517268myo-inositol-assimilation
6677517306maltose-assimilation
6677517634D-glucose-assimilation
6677517716lactose-assimilation
6677517814salicin-assimilation
6677517924D-sorbitol-assimilation
6677517992sucrose-assimilation
6677518222xylose-assimilation
6677518403L-arabitol-assimilation
6677522599arabinose-assimilation
6677527082trehalose-assimilation
6677528017starch-assimilation
6677528053melibiose-assimilation
6677528066gentiobiose-assimilation
6677528087glycogen-assimilation
6677528847D-fucose-assimilation
6677532032potassium gluconate-assimilation
6677532528turanose-assimilation
6677559640N-acetylglucosamine-assimilation
6677562318D-lyxose-assimilation
6677574863methyl beta-D-xylopyranoside-assimilation
66775320061methyl alpha-D-glucopyranoside-assimilation
66775esculin ferric citrate-assimilation
66775potassium 5-dehydro-D-gluconate-assimilation
6677515824D-fructose+assimilation
6677516899D-mannitol+assimilation
6677517266L-sorbose+assimilation
6677517754glycerol+assimilation
6677518287L-fucose+assimilation
6677518305arbutin+assimilation
6677518333D-arabitol+assimilation
6677527613amygdalin+assimilation
6677562345L-rhamnose+assimilation
66775potassium 2-dehydro-D-gluconate+assimilation
6677516991dna-hydrolysis
6677517029chitin-hydrolysis
6677528017starch-hydrolysis
6677553424tween 20-hydrolysis
66775casein-hydrolysis
66775crystalline cellulose-hydrolysis
667754853esculin+hydrolysis
6677553426tween 80+hydrolysis

metabolite production

@refChebi-IDmetaboliteproduction
6677516136hydrogen sulfideno
6677535581indoleno

enzymes

@refvalueactivityec
66775alpha-chymotrypsin-3.4.21.1
66775alpha-fucosidase-3.2.1.51
66775alpha-galactosidase-3.2.1.22
66775alpha-glucosidase-3.2.1.20
66775alpha-mannosidase-3.2.1.24
66775arginine dihydrolase-3.5.3.6
66775beta-galactosidase-3.2.1.23
66775beta-glucosidase-3.2.1.21
66775beta-glucuronidase-3.2.1.31
66775cystine arylamidase-3.4.11.3
66775gelatinase-
66775lipase (C 14)-
66775N-acetyl-beta-glucosaminidase-3.2.1.52
66775naphthol-AS-BI-phosphohydrolase-
66775nitrate reductase-1.7.99.4
66775trypsin-3.4.21.4
66775urease-3.5.1.5
66775acid phosphatase+3.1.3.2
66775alkaline phosphatase+3.1.3.1
66775beta-galactosidase+3.2.1.23
66775catalase+1.11.1.6
66775cytochrome oxidase+1.9.3.1
66775esterase (C 4)+
66775esterase Lipase (C 8)+
66775leucine arylamidase+3.4.11.1
66775valine arylamidase+

fatty acid profile

  • fatty acids

    @reffatty acidpercentage
    66775C14:0 3OH and/or iso-C16:1 I2
    66775C18:02
    66775C16:012.6
    66775C12:1 3OH2.3
    66775C10:0 3OH3.8
    66775C16:1 w6c and/or C16:1 w7c30.8
    66775C18:1 w6c and/or C18:1 w7c46.4
  • type of FA analysis: whole cell analysis
  • incubation medium: Marine agar
  • incubation temperature: 15
  • software version: Sherlock 6.1
  • library/peak naming table: TSBA6
  • system: MIS MIDI

Isolation, sampling and environmental information

isolation

  • @ref: 66775
  • sample type: surface seawater sample of the Arctic Circle
  • geographic location: Kongsfjorden, Svalbard
  • country: Arctic Ocean
  • latitude: 79.0038
  • longitude: 11.6691
  • enrichment culture: Reasoner's 2A (R2A) agar (BD Diagnostics)
  • enrichment culture composition: prepared in aged seawater
  • enrichment culture duration: 2 months
  • enrichment culture temperature: 10
  • isolation procedure: by a standard dilution-plating method. The optimum temperature for growth was determined and the working cultures were thereafter maintained on marine agar 2216 (MA; BD Diagnostics) or in marine broth 2216 (MB; BD Diagnostics) at 15 C.

isolation source categories

Cat1Cat2Cat3
#Environmental#Aquatic#Marine
#Climate#Cold#Polar
#Environmental#Aquatic#Surface water

taxonmaps

  • @ref: 69479
  • File name: preview.99_87826.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_473;96_20019;97_35394;98_52157;99_87826&stattab=map
  • Last taxonomy: Leucothrix arctica
  • 16S sequence: KJ160496
  • Sequence Identity:
  • Total samples: 884
  • soil counts: 10
  • aquatic counts: 696
  • animal counts: 177
  • plant counts: 1

Sequence information

16S sequences

  • @ref: 66775
  • description: Leucothrix sp. IMCC9719 16S ribosomal RNA gene, partial sequence
  • accession: KJ160496
  • length: 1449
  • database: ena
  • NCBI tax ID: 1481894

GC content

  • @ref: 66775
  • GC-content: 43.5
  • method: genome sequence analysis

External links

@ref: 66775

culture collection no.: KACC 18010, NBRC 110382, IMCC 9719

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny30516460Leucothrix arctica sp. nov., isolated from Arctic seawater.Baek K, Choi A, Lee YM, Lee HK, Cho JCInt J Syst Evol Microbiol10.1099/ijsem.0.0030722018Arctic Regions, Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Fatty Acids/chemistry, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Seawater/*microbiology, Sequence Analysis, DNA, Thiotrichaceae/*classification/genetics, Ubiquinone/chemistryTranscriptome
Phylogeny31486761Leucothrix sargassi sp. nov., isolated from a marine alga [Sargassum natans (L.) Gaillon].Liu T, Zhang Y, Zhang X, Zhou L, Meng C, Zhou C, Jing C, Zhu W, Wang YInt J Syst Evol Microbiol10.1099/ijsem.0.0036942019Bacterial Typing Techniques, Base Composition, China, DNA, Bacterial/genetics, Fatty Acids/chemistry, Nucleic Acid Hybridization, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sargassum/*microbiology, Sequence Analysis, DNA, Thiotrichaceae/*classification/isolation & purification, Ubiquinone/chemistryTranscriptome

Reference

@idauthorstitledoi/urljournalpubmed
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
66775Kiwoon Baek, Ahyoung Choi, Yung Mi Lee, Hong Kum Lee and Jang-Cheon ChoLeucothrix arctica sp. nov., isolated from Arctic seawater10.1099/ijsem.0.003072IJSEM 68: 3851-3855 201830516460
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.MicrobeAtlas 1.0 betahttps://microbeatlas.org/