Strain identifier
BacDive ID: 159785
Type strain: 
Species: Clostridium neonatale
Strain Designation: St. Boniface Hospital 30686
NCBI tax ID(s): 137838 (species)
version 8.1 (current version)
General
@ref: 66587
BacDive-ID: 159785
keywords: Bacteria, obligate anaerobe, spore-forming, Gram-positive, motile, rod-shaped
description: Clostridium neonatale St. Boniface Hospital 30686 is an obligate anaerobe, spore-forming, Gram-positive bacterium that was isolated from the blood culture of a neonate with necrotizing enterocolitis from the spleen of a 3-month-old.
NCBI tax id
- NCBI tax id: 137838
- Matching level: species
doi: 10.13145/bacdive159785.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/bacillota
- domain: Bacteria
- phylum: Bacillota
- class: Clostridia
- order: Eubacteriales
- family: Clostridiaceae
- genus: Clostridium
- species: Clostridium neonatale
- full scientific name: Clostridium neonatale Bernard et al. 2018
@ref: 66587
domain: Bacteria
phylum: Firmicutes
class: Clostridia
order: Eubacteriales
family: Clostridiaceae
genus: Clostridium
species: Clostridium neonatale
strain designation: St. Boniface Hospital 30686
type strain: yes
Morphology
cell morphology
- @ref: 66587
- gram stain: positive
- cell shape: rod-shaped
- motility: yes
colony morphology
- @ref: 66587
- colony color: grey
Physiology and metabolism
oxygen tolerance
- @ref: 66587
- oxygen tolerance: obligate anaerobe
spore formation
- @ref: 66587
- spore description: discernible subterminal spores
- spore formation: yes
observation
| @ref | observation |
|---|---|
| 66587 | Colonies are grey, irregular-edged and spreading or swarming on BHIA and are non-haemolytic on BBA. |
| 66587 | Growth in PY broth may be enhanced by dextrose. |
| 66587 | Milk is digested with gas production. |
metabolite utilization
| @ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
|---|---|---|---|---|
| 66587 | 6731 | melezitose | + | assimilation |
| 66587 | 26546 | rhamnose | - | fermentation |
| 66587 | 27082 | trehalose | - | fermentation |
| 66587 | 29864 | mannitol | - | fermentation |
| 66587 | 30911 | sorbitol | - | fermentation |
| 66587 | 16634 | raffinose | + | fermentation |
| 66587 | 17057 | cellobiose | + | fermentation |
| 66587 | 17234 | glucose | + | fermentation |
| 66587 | 17306 | maltose | + | fermentation |
| 66587 | 17716 | lactose | + | fermentation |
| 66587 | 17814 | salicin | + | fermentation |
| 66587 | 18222 | xylose | + | fermentation |
| 66587 | 28017 | starch | + | fermentation |
| 66587 | 28053 | melibiose | + | fermentation |
| 66587 | 28757 | fructose | + | fermentation |
| 66587 | 33942 | ribose | + | fermentation |
| 66587 | 37684 | mannose | + | fermentation |
| 66587 | 5291 | gelatin | - | hydrolysis |
| 66587 | 4853 | esculin | + | hydrolysis |
| 66587 | 28017 | starch | + | hydrolysis |
| 66587 | 16411 | acetic acid | - | reduction |
| 66587 | 35581 | indole | - | reduction |
| 66587 | 17632 | nitrate | - | reduction |
| 66587 | 17968 | butyrate | - | reduction |
| 66587 | 24996 | lactate | - | reduction |
metabolite production
| @ref | Chebi-ID | metabolite | production |
|---|---|---|---|
| 66587 | 16411 | acetic acid | yes |
| 66587 | 35581 | indole | no |
enzymes
| @ref | value | activity | ec |
|---|---|---|---|
| 66587 | catalase | - | 1.11.1.6 |
| 66587 | cytochrome oxidase | - | 1.9.3.1 |
| 66587 | lecithinase | - | |
| 66587 | lipase | - | |
| 66587 | urease | - | 3.5.1.5 |
Isolation, sampling and environmental information
isolation
- @ref: 66587
- sample type: the blood culture of a neonate with necrotizing enterocolitis (NEC) from the spleen of a 3-month-old
- geographic location: Winnipeg
- country: Canada
- origin.country: CAN
- continent: North America
- latitude: 49.8842
- longitude: -97.1248
isolation source categories
| Cat1 | Cat2 | Cat3 |
|---|---|---|
| #Infection | #Patient | #Blood culture |
| #Host | #Human | #Child |
| #Infection | #Disease |
Sequence information
GC content
- @ref: 66587
- GC-content: 28.4-28.5
- method: genome sequence analysis
External links
@ref: 66587
culture collection no.: ATCC BAA 265, CCUG 46077, LCDC 99A005
straininfo link
- @ref: 112169
- straininfo: 409885
literature
- topic: Phylogeny
- Pubmed-ID: 29889020
- title: Clostridium neonatale sp. nov. linked to necrotizing enterocolitis in neonates and a clarification of species assignable to the genus Clostridium (Prazmowski 1880) emend. Lawson and Rainey 2016.
- authors: Bernard K, Burdz T, Wiebe D, Alfa M, Bernier AM
- journal: Int J Syst Evol Microbiol
- DOI: 10.1099/ijsem.0.002827
- year: 2018
- mesh: Bacterial Typing Techniques, Base Composition, Clostridium/*classification/genetics, DNA, Bacterial/genetics, Enterocolitis, Necrotizing/*microbiology, Humans, Infant, Newborn, Microbial Sensitivity Tests, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
- topic2: Pathogenicity
Reference
| @id | authors | title | doi/url | journal | pubmed |
|---|---|---|---|---|---|
| 20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | 10.1099/ijsem.0.004332 | ||
| 66587 | Kathryn Bernard, Tamara Burdz, Deborah Wiebe, Michelle Alfa, Anne-Marie Bernier | Salegentibacter sediminis sp. nov., a marine bacterium of the family lavobacteriaceaeClostridium neonatale sp. nov. linked to necrotizing enterocolitis in neonates and a clarification of species assignable to the genus Clostridium (Prazmowski 1880) emend. Lawson and Rainey 2016 | 10.1099/ijsem.0.002827 | IJSEM 68: 2416-2423 2018 | 29889020 |
| 112169 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | StrainInfo: A central database for resolving microbial strain identifiers | 10.60712/SI-ID409885.1 |