Strain identifier

BacDive ID: 159750

Type strain: Yes

Species: Flavobacterium chryseum

Strain Designation: P3160

NCBI tax ID(s): 2301192 (species)

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General

@ref: 66678

BacDive-ID: 159750

keywords: 16S sequence, Bacteria, aerobe, mesophilic, Gram-positive, motile, rod-shaped, pigmented

description: Flavobacterium chryseum P3160 is an aerobe, mesophilic, Gram-positive bacterium that has a yellow pigmentation and was isolated from stone fragment.

NCBI tax id

  • NCBI tax id: 2301192
  • Matching level: species

doi: 10.13145/bacdive159750.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacteroidota
  • domain: Bacteria
  • phylum: Bacteroidota
  • class: Flavobacteriia
  • order: Flavobacteriales
  • family: Flavobacteriaceae
  • genus: Flavobacterium
  • species: Flavobacterium chryseum
  • full scientific name: Flavobacterium chryseum Králová et al. 2018

@ref: 66678

domain: Bacteria

phylum: Bacteroidetes

class: Flavobacteriia

order: Flavobacteriales

family: Flavobacteriaceae

genus: Flavobacterium

species: Flavobacterium chryseum

strain designation: P3160

type strain: yes

Morphology

cell morphology

  • @ref: 66678
  • gram stain: positive
  • cell length: 2.2-4.8 µm
  • cell width: 0.4-0.8 µm
  • cell shape: rod-shaped
  • motility: yes
  • flagellum arrangement: gliding

pigmentation

  • @ref: 66678
  • production: yes
  • color: yellow
  • name: flexirubin-type

Culture and growth conditions

culture medium

@refnamegrowth
66678MacConkey agarno
66678Marine agar (MA)no
66678Nutrient agar (NA)yes
66678Reasoner's 2A agar (R2A)yes
66678tryptic soy agar (TSA)yes

culture temp

@refgrowthtypetemperaturerange
66678nogrowth35mesophilic
66678positivegrowth1-25

culture pH

  • @ref: 66678
  • ability: positive
  • type: growth
  • pH: 6-9
  • PH range: alkaliphile

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
66678aerobe
66678microaerophile

spore formation

  • @ref: 66678
  • spore formation: no

halophily

@refsaltgrowthtested relationconcentration
66678NaClpositivegrowth1 %(w/v)
66678NaClnogrowth2 %(w/v)

observation

@refobservation
66678Cells occur singly, occasionally in pairs.
66678Presence of a capsule is negative.
66678Gliding motility present on R2A agar, but no motility is observed by hanging-drop technique
66678Growth on Endo agar is strain-dependent.
66678Glucose is not fermented to acid in OF test medium.

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6667815792malonate-assimilation
6667827856acetamide-assimilation
6667853258citric acid-assimilation
6667817234glucose+builds acid from
6667817306maltose+builds acid from
6667818222xylose+builds acid from
6667828757fructose+builds acid from
6667813705acetoacetate-carbon source
6667814314D-glucose 6-phosphate-carbon source
6667815740formate-carbon source
6667815748D-glucuronate-carbon source
6667815971L-histidine-carbon source
6667816467L-arginine-carbon source
6667816523D-serine-carbon source
6667816537galactarate-carbon source
6667816651(S)-lactate-carbon source
66678167632-oxobutanoate-carbon source
6667816865gamma-aminobutyric acid-carbon source
6667816899D-mannitol-carbon source
6667816977L-alanine-carbon source
6667817115L-serine-carbon source
6667817164stachyose-carbon source
6667817268myo-inositol-carbon source
6667817272propionate-carbon source
6667817596inosine-carbon source
6667817716lactose-carbon source
6667817754glycerol-carbon source
6667817924D-sorbitol-carbon source
66678181014-hydroxyphenylacetic acid-carbon source
6667818183L-pyroglutamic acid-carbon source
6667818287L-fucose-carbon source
6667818333D-arabitol-carbon source
6667826490quinate-carbon source
6667828053melibiose-carbon source
6667828847D-fucose-carbon source
6667829990D-aspartate-carbon source
6667829991L-aspartate-carbon source
6667830612D-glucarate-carbon source
6667830796(R)-malic acid-carbon source
6667830797(S)-malic acid-carbon source
6667832323glucuronamide-carbon source
6667832528turanose-carbon source
6667835418n-acetylneuraminate-carbon source
66678370543-hydroxybutyrate-carbon source
6667848607lithium chloride-carbon source
6667851850methyl pyruvate-carbon source
6667853258citric acid-carbon source
6667862345L-rhamnose-carbon source
6667863153N-acetyl-D-mannosamine-carbon source
6667864103sodium butyrate-carbon source
66678645522-hydroxybutyrate-carbon source
6667871321fusidate-carbon source
6667873706bromosuccinate-carbon source
66678739183-O-methyl-D-glucose-carbon source
6667874611methyl (R)-lactate-carbon source
6667875229sodium bromate-carbon source
6667875248potassium tellurite-carbon source
6667878697D-fructose 6-phosphate-carbon source
66678100147nalidixic acid-carbon source
66678161680aztreonam-carbon source
666781 % sodium lactate-carbon source
66678168102-oxoglutarate-carbon source
666785291gelatin+carbon source
6667812936D-galactose+carbon source
6667815824D-fructose+carbon source
6667816024D-mannose+carbon source
6667816411acetic acid+carbon source
6667816551D-trehalose+carbon source
6667816634raffinose+carbon source
6667817057cellobiose+carbon source
6667817306maltose+carbon source
6667817309pectin+carbon source
6667817464L-galactonic acid gamma-lactone+carbon source
6667817814salicin+carbon source
6667817925alpha-D-glucose+carbon source
6667817992sucrose+carbon source
6667818024D-galacturonic acid+carbon source
6667823652dextrin+carbon source
6667828066gentiobiose+carbon source
6667828800N-acetylgalactosamine+carbon source
6667829985L-glutamate+carbon source
6667853423tween 40+carbon source
6667859640N-acetylglucosamine+carbon source
6667870744glycine-proline+carbon source
66678320055methyl beta-D-glucopyranoside+carbon source
6667862968cellulose-degradation
6667853426tween 80-hydrolysis
6667861995lecithin-hydrolysis
666784853esculin+hydrolysis
666785291gelatin+hydrolysis
6667816991dna+hydrolysis
6667828017starch+hydrolysis
66678casein+hydrolysis
6667816301nitrite-reduction
6667817632nitrate-reduction

antibiotic resistance

@refChEBImetaboliteis antibioticis sensitivesensitivity conc.
6667828971ampicillinyesyes10 µg (disc)
6667817698chloramphenicolyesyes30 µg (disc)
6667818208penicillin gyesyes10 µg (disc)
6667827902tetracyclineyesyes30 µg (disc)
6667873908vibriostatyesyes150 µg (disc)

metabolite production

  • @ref: 66678
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

enzymes

@refvalueactivityec
66678alpha-chymotrypsin-3.4.21.1
66678alpha-fucosidase-3.2.1.51
66678alpha-galactosidase-3.2.1.22
66678alpha-mannosidase-3.2.1.24
66678arginine dihydrolase-3.5.3.6
66678beta-glucuronidase-3.2.1.31
66678cystine arylamidase-3.4.11.3
66678lipase (C 14)-
66678lysine decarboxylase-4.1.1.18
66678N-acetyl-beta-glucosaminidase-3.2.1.52
66678ornithine decarboxylase-4.1.1.17
66678trypsin-3.4.21.4
66678urease-3.5.1.5
66678acid phosphatase+3.1.3.2
66678alkaline phosphatase+3.1.3.1
66678beta-glucosidase+3.2.1.21
66678catalase+1.11.1.6
66678cytochrome oxidase+1.9.3.1
66678esterase Lipase (C 8)+
66678leucine arylamidase+3.4.11.1
66678naphthol-AS-BI-phosphohydrolase+
66678valine arylamidase+

fatty acid profile

  • fatty acids

    @reffatty acidpercentage
    66678C16:0 3OH1
    66678C16:0 iso2
    66678C17:0 iso 3OH10
    66678C15:0 2OH1.1
    66678C15:1 anteiso A1.2
    66678C16:1 iso H1.5
    66678C17:0 2OH1.6
    66678C16:01.8
    66678C15:0 iso18.6
    66678C16:0 iso 3OH4.5
    66678C17:1 w6c4.6
    66678C15:0 anteiso6.4
    66678C15:1 w6c6.5
    66678C16:0 10-methyl/ C17:1 iso w9c6.9
    66678C15:0 iso 3OH8.7
    66678C15:1 iso G9.5
    66678C16:1 w7c/ C16:1 w6c9.8
  • type of FA analysis: whole cell analysis
  • incubation temperature: 20
  • incubation time: 2
  • software version: Sherlock 6.2
  • library/peak naming table: RTSBA6.21
  • system: MIS MIDI
  • cutoff value:

Isolation, sampling and environmental information

isolation

  • @ref: 66678
  • sample type: stone fragment
  • sampling date: 2009-2014
  • geographic location: Devils Rocks, James Ross Island
  • country: Antarctica
  • origin.country: ATA
  • continent: Australia and Oceania
  • latitude: -63.8527
  • longitude: -57.8097
  • enrichment culture: R2A agar
  • enrichment culture duration: 5-7 days
  • enrichment culture temperature: 15
  • isolation procedure: 1 g stone fragments or soil samples in 5 ml sterile saline solution, subsequently 100 µl of this suspension was spread by L-loop on the surface of Reasoner's 2A agar plates and cultivated at 15°C for up to 5-7 days. Analogously, water samples were spread on R2A agar plates (150 µl) and cultivated at 15°C for up to 5 days.

isolation source categories

  • Cat1: #Environmental
  • Cat2: #Terrestrial
  • Cat3: #Geologic

taxonmaps

  • @ref: 69479
  • File name: preview.99_2370.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_66;96_352;97_396;98_1240;99_2370&stattab=map
  • Last taxonomy: Flavobacterium
  • 16S sequence: MH100900
  • Sequence Identity:
  • Total samples: 2507
  • soil counts: 813
  • aquatic counts: 969
  • animal counts: 657
  • plant counts: 68

Sequence information

16S sequences

  • @ref: 66678
  • description: Flavobacterium luteum strain P3160 16S ribosomal RNA gene, partial sequence
  • accession: MH100900
  • length: 1357
  • database: ena
  • NCBI tax ID: 2301192

External links

@ref: 66678

culture collection no.: LMG 30615, CCM 8826

literature

  • topic: Phylogeny
  • Pubmed-ID: 30095387
  • title: Flavobacterium chryseum sp. nov. and Flavobacterium psychroterrae sp. nov., novel environmental bacteria isolated from Antarctica.
  • authors: Kralova S, Svec P, Busse HJ, Stankova E, Vaczi P, Sedlacek I
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijsem.0.002952
  • year: 2018
  • mesh: Antarctic Regions, Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Fatty Acids/chemistry, Flavobacterium/*classification/genetics/isolation & purification, Nucleic Acid Hybridization, Phosphatidylethanolamines/chemistry, *Phylogeny, Pigmentation, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, *Soil Microbiology, Spermidine/analogs & derivatives/chemistry, Vitamin K 2/analogs & derivatives/chemistry
  • topic2: Transcriptome

Reference

@idauthorstitledoi/urljournalpubmed
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
66678Stanislava Kralova, Pavel Svec, Hans-Jürgen Busse, Eva Stankova, Peter Vaczi, Ivo SedlacekFlavobacterium chryseum sp. nov. and Flavobacterium psychroterrae sp. nov., novel environmental bacteria isolated from Antarctica10.1099/ijsem.0.002952IJSEM 68: 3132-3139 201830095387
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.MicrobeAtlas 1.0 betahttps://microbeatlas.org/