Strain identifier

BacDive ID: 159748

Type strain: Yes

Species: Lactobacillus porci

Strain history: <- Doo-Sang Park, KRIBB

NCBI tax ID(s): 2012477 (species)

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General

@ref: 66676

BacDive-ID: 159748

keywords: 16S sequence, Bacteria, facultative anaerobe, mesophilic, Gram-positive, rod-shaped, colony-forming

description: Lactobacillus porci KCTC 21090 is a facultative anaerobe, mesophilic, Gram-positive bacterium that forms circular colonies and was isolated from small intestine of a 6-month-old female swine fed a mixed fodder.

NCBI tax id

  • NCBI tax id: 2012477
  • Matching level: species

strain history

  • @ref: 67771
  • history: <- Doo-Sang Park, KRIBB

doi: 10.13145/bacdive159748.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Lactobacillales
  • family: Lactobacillaceae
  • genus: Lactobacillus
  • species: Lactobacillus porci
  • full scientific name: Lactobacillus porci Kim et al. 2018

@ref: 66676

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Lactobacillales

family: Lactobacillaceae

genus: Lactobacillus

species: Lactobacillus porci

type strain: yes

Morphology

cell morphology

@refgram staincell lengthcell widthcell shapemotility
66676positive3.5-5.5 µm0.5-0.7 µmrod-shapedno
67771rod-shapedno
67771positive

colony morphology

  • @ref: 66676
  • colony size: 2-2.5 mm
  • colony color: beige
  • colony shape: circular
  • incubation period: 2 days
  • medium used: de Man, Rogosa and Sharpe

Culture and growth conditions

culture medium

  • @ref: 66676
  • name: de Man, Rogosa and Sharpe
  • growth: yes

culture temp

@refgrowthtypetemperaturerange
66676positiveoptimum37mesophilic
66676positivegrowth15-50
67771positivegrowth37mesophilic

culture pH

@refabilitytypepHPH range
66676positiveoptimum7
66676positivegrowth5-11alkaliphile

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
66676facultative anaerobe
67771facultative anaerobe

spore formation

  • @ref: 66676
  • spore formation: no

halophily

@refsaltgrowthtested relationconcentration
66676NaClpositivegrowth0-1 %(w/v)
66676NaClnogrowth10 %(w/v)
66676NaClnogrowth>1 %(w/v)

observation

@refobservation
66676Cells occurring singly or in pairs
66676D-Lactic acid is produced as the end product of glucose metabolism, indicating that strain SG816T is homofermentative.

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6667612936D-galactose-builds acid from
6667616443D-tagatose-builds acid from
6667617057cellobiose-builds acid from
6667617814salicin-builds acid from
6667618305arbutin-builds acid from
6667624265gluconate-builds acid from
6667627613amygdalin-builds acid from
6667628017starch-builds acid from
6667628053melibiose-builds acid from
6667628066gentiobiose-builds acid from
6667629864mannitol-builds acid from
6667630849L-arabinose-builds acid from
6667633942ribose-builds acid from
6667659640N-acetylglucosamine-builds acid from
6667665327D-xylose-builds acid from
666764853esculin+builds acid from
6667615824D-fructose+builds acid from
6667616024D-mannose+builds acid from
6667616634raffinose+builds acid from
6667617306maltose+builds acid from
6667617634D-glucose+builds acid from
6667617716lactose+builds acid from
6667617992sucrose+builds acid from
6667627082trehalose+builds acid from

enzymes

@refvalueactivityec
66676alpha-glucosidase-3.2.1.20
66676beta-glucosidase-3.2.1.21
66676catalase-1.11.1.6
66676naphthol-AS-BI-phosphohydrolase-
66676alpha-galactosidase+3.2.1.22
66676beta-galactosidase+3.2.1.23
66676leucine arylamidase+3.4.11.1

Isolation, sampling and environmental information

isolation

@refsample typecountryorigin.countrycontinentenrichment cultureenrichment culture durationisolation proceduregeographic location
66676small intestine of a 6-month-old female swine fed a mixed fodderRepublic of KoreaKORAsiade Man, Rogosa and Sharpe agar2 daysFor isolation, intestinal content was resuspended and serially diluted in sterilized 0.85 % NaCl, followed by cultivation onto de Man, Rogosa and Sharpe agar under anaerobic conditions with 86 % N2, 7 % CO2, and 7 % H2. After 48 h of incubation, a single ivory and convex colony was obtained.
67771From gastrointestinal tract(the small intestine) of swineRepublic of KoreaKORAsiaGimje, Jellabuk-do

isolation source categories

Cat1Cat2Cat3
#Host Body-Site#Gastrointestinal tract#Small intestine
#Host#Mammals#Suidae (Pig,Swine)

Sequence information

16S sequences

  • @ref: 66676
  • description: Lactobacillus sp. SG816 16S ribosomal RNA gene, partial sequence
  • accession: MF346092
  • length: 1474
  • database: ena
  • NCBI tax ID: 2012477

GC content

@refGC-contentmethod
6667651.5high performance liquid chromatography (HPLC)
6777151.5

External links

@ref: 66676

culture collection no.: KCTC 21090, NBRC 112917, SG 816

literature

  • topic: Phylogeny
  • Pubmed-ID: 30117800
  • title: Lactobacillus porci sp. nov., isolated from small intestine of a swine.
  • authors: Kim JS, Choe H, Kim KM, Lee YR, Rhee MS, Park DS
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijsem.0.002949
  • year: 2018
  • mesh: Animals, Bacterial Typing Techniques, Base Composition, Cell Wall/chemistry, DNA, Bacterial/genetics, DNA-Directed RNA Polymerases/genetics, Fatty Acids/chemistry, Fermentation, Genes, Bacterial, Intestine, Small/*microbiology, Lactic Acid/chemistry, Lactobacillus/*classification/genetics/isolation & purification, Peptidoglycan/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Republic of Korea, Sequence Analysis, DNA, Swine/*microbiology
  • topic2: Transcriptome

Reference

@idauthorstitledoi/urljournalpubmed
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
66676Ji-Sun Kim, Hanna Choe, Kyung Mo Kim, Yu-Ri Lee, Moon-Soo Rhee, Doo-Sang ParkLactobacillus porci sp. nov., isolated from small intestine of a swine10.1099/ijsem.0.002949IJSEM 68: 3118-3124 201830117800
67771Curators of the KCTChttps://kctc.kribb.re.kr/En/Kctc