Strain identifier

BacDive ID: 159747

Type strain: Yes

Species: Actinomycetospora endophytica

Strain Designation: A-T 8314

NCBI tax ID(s): 2291215 (species)

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@ref: 66667

BacDive-ID: 159747

keywords: 16S sequence, Bacteria, aerobe, spore-forming, Gram-positive

description: Actinomycetospora endophytica A-T 8314 is an aerobe, spore-forming, Gram-positive bacterium that was isolated from roots of orchid .

NCBI tax id

  • NCBI tax id: 2291215
  • Matching level: species

doi: 10.13145/bacdive159747.20230509.8

Name and taxonomic classification


  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Pseudonocardiales
  • family: Pseudonocardiaceae
  • genus: Actinomycetospora
  • species: Actinomycetospora endophytica
  • full scientific name: Actinomycetospora endophytica Sakdapetsiri et al. 2018

@ref: 66667

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Pseudonocardiales

family: Pseudonocardiaceae

genus: Actinomycetospora

species: Actinomycetospora endophytica

strain designation: A-T 8314

type strain: yes


cell morphology

  • @ref: 66667
  • gram stain: positive

colony morphology

  • @ref: 66667
  • colony color: white

multicellular morphology

  • @ref: 66667
  • forms multicellular complex: yes
  • complex color: pale yellow
  • further description: the substrate mycelium develops long, branches.

Culture and growth conditions

culture medium

66667ISP 2yes
66667ISP 3yes
66667ISP 5yes
66667ISP 7yes

culture temp


culture pH

@refabilitytypepHPH range

Physiology and metabolism

oxygen tolerance

  • @ref: 66667
  • oxygen tolerance: aerobe

spore formation

  • @ref: 66667
  • spore description: bud-shaped spores on aerial mycelium.
  • type of spore: spore
  • spore formation: yes


  • @ref: 66667
  • salt: NaCl
  • growth: positive
  • tested relation: growth
  • concentration: 0.5-8 %

metabolite utilization

@refmetaboliteutilization activitykind of utilization testedChebi-ID
66667D-fructose-carbon source15824
66667D-mannose-carbon source16024
66667raffinose-carbon source16634
66667cellobiose-carbon source17057
66667erythritol-carbon source17113
66667xylitol-carbon source17151
66667myo-inositol-carbon source17268
66667lactose-carbon source17716
66667glycerol-carbon source17754
66667D-sorbitol-carbon source17924
66667xylose-carbon source18222
66667L-arabitol-carbon source18403
66667arabinose-carbon source22599
66667trehalose-carbon source27082
66667sorbose-carbon source27922
66667D-mannitol+carbon source16899
66667maltose+carbon source17306
66667D-glucose+carbon source17634
66667rhamnose+carbon source26546


66667acid phosphatase+
66667alkaline phosphatase+
66667cystine arylamidase+
66667esterase (C 4)+
66667esterase Lipase (C 8)+
66667leucine arylamidase+
66667valine arylamidase+

fatty acid profile

  • fatty acids

    @reffatty acidpercentage
    66667C16:0 iso H3
    66667C10:0 iso1.1
    66667C17:0 iso1.4
    66667C17:0 anteiso3.1
    66667C14:0 iso3.4
    66667C15:0 iso3.8
    66667C16:0 iso50.2
    66667C17:1 anteiso w8c8.7
  • type of FA analysis: whole cell analysis
  • incubation medium: glucose-yeast extract broth
  • agar/liquid: liquid
  • incubation temperature: 28
  • incubation time: 7
  • software version: Sherlock 6.2B
  • library/peak naming table: TSBA6
  • system: MIS MIDI
  • cutoff value:

Isolation, sampling and environmental information


  • @ref: 66667
  • sample type: roots of orchid (Podochilus microphyllus Lindl.)
  • host species: Podochilus microphyllus
  • geographic location: Trat Province
  • country: Thailand
  • THA
  • continent: Asia
  • latitude: 12.6183
  • longitude: 102.577
  • enrichment culture: humic acid-vitamin agar
  • enrichment culture composition: supplemented with 50 mg l-1 of nalidixic acid, 100 mg l-1 of cycloheximide and 2 mg l-1 of terbinafine
  • enrichment culture duration: 21 days
  • enrichment culture temperature: 28
  • isolation procedure: The roots were surface sterilized as follows: sequential immersion in 70 % (v/v) ethanol for 5 min and sodium hypochlorite solution (4 % available chloride, freshly prepared) for 5 min. Then the sample was washed three times with sterile distilled water to remove surface sterilization agents and soaked in 10 % NaHCO3 solution for 10 min to eliminate growth of endophytic fungi. One gram of the sample was cut into small pieces with sterilized scissors and then crushed with mortar and pestle in 9 ml sterile sodium dodecyl sulfate (SDS) solution (0.1 g SDS, 1.75 g KH2PO4, 3.5 g K2HPO4, 1 l distilled water), 10-fold serially diluted in SDS solution and plated on humic acid-vitamin agar and incubated at 28°C for 21 days.

isolation source categories

#Host#Plants#Herbaceous plants (Grass,Crops)
#Host Body-Site#Plant#Root (Rhizome)

Sequence information

16S sequences

  • @ref: 66667
  • description: Actinomycetospora sp. strain A-T 8314 16S ribosomal RNA gene, partial sequence
  • accession: MH071353
  • length: 1458
  • database: ena
  • NCBI tax ID: 2291215

Genome sequences

  • @ref: 66792
  • description: Actinomycetospora endophytica TBRC 5722
  • accession: GCA_021026375
  • assembly level: contig
  • database: ncbi
  • NCBI tax ID: 2291215

GC content

  • @ref: 66667
  • GC-content: 73-73.4
  • method: high performance liquid chromatography (HPLC)

External links

@ref: 66667

culture collection no.: TBRC 5722, NBRC 113235


Phylogeny30059002Actinomycetospora endophytica sp. nov., isolated from wild orchid (Podochilus microphyllus Lindl.) in Thailand.Sakdapetsiri C, Ngaemthao W, Suriyachadkun C, Duangmal K, Kitpreechavanich VInt J Syst Evol Microbiol10.1099/ijsem.0.0029382018Actinomycetales/*classification/genetics/isolation & purification, Bacterial Typing Techniques, Base Composition, Cell Wall/chemistry, DNA, Bacterial/genetics, Diaminopimelic Acid/chemistry, Fatty Acids/chemistry, Nucleic Acid Hybridization, Orchidaceae/*microbiology, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Thailand, Vitamin K 2/analogs & derivatives/chemistryTranscriptome
Phylogeny35851075Actinomycetospora soli sp. nov., isolated from the rhizosphere soil of Averrhoa carambola L.Chantavorakit T, Duangmal KInt J Syst Evol Microbiol10.1099/ijsem.0.0052772022*Actinomycetales, *Averrhoa, Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Fatty Acids/chemistry, Phylogeny, RNA, Ribosomal, 16S/genetics, Rhizosphere, Sequence Analysis, DNA, Soil, Soil Microbiology, ThailandTranscriptome


20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
66667Chatsuda Sakdapetsiri, Wipaporn Ngaemthao, Chanwit Suriyachadkun, Kannika Duangmal, Vichien KitpreechavanichActinomycetospora endophytica sp. nov., isolated from wild orchid (Podochilus microphyllus Lindl.) in Thailand10.1099/ijsem.0.002938IJSEM 68: 3017-3021 201830059002
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)