Strain identifier
BacDive ID: 159739
Type strain:
Species: Clostridium bovifaecis
Strain Designation: BXX
Strain history: B. Fu; Sch. of Environ. & Civil Engineering, Jiangnan Univ., China; BXX.
NCBI tax ID(s): 2184719 (species)
General
@ref: 66658
BacDive-ID: 159739
keywords: genome sequence, 16S sequence, Bacteria, anaerobe, spore-forming, mesophilic, Gram-positive, motile, rod-shaped, colony-forming
description: Clostridium bovifaecis BXX is an anaerobe, spore-forming, mesophilic bacterium that forms circular colonies and was isolated from cow manure.
NCBI tax id
- NCBI tax id: 2184719
- Matching level: species
strain history
- @ref: 67770
- history: B. Fu; Sch. of Environ. & Civil Engineering, Jiangnan Univ., China; BXX.
doi: 10.13145/bacdive159739.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/bacillota
- domain: Bacteria
- phylum: Bacillota
- class: Clostridia
- order: Eubacteriales
- family: Clostridiaceae
- genus: Clostridium
- species: Clostridium bovifaecis
- full scientific name: Clostridium bovifaecis Zhu et al. 2018
@ref: 66658
domain: Bacteria
phylum: Firmicutes
class: Clostridia
order: Eubacteriales
family: Clostridiaceae
genus: Clostridium
species: Clostridium bovifaecis
strain designation: BXX
type strain: yes
Morphology
cell morphology
- @ref: 66658
- gram stain: positive
- cell shape: rod-shaped
- motility: yes
colony morphology
- @ref: 66658
- colony color: cream
- colony shape: circular
- medium used: DSMZ medium 311c agar
Culture and growth conditions
culture medium
- @ref: 66658
- name: DSMZ medium 311c agar
- growth: yes
culture temp
@ref | growth | type | temperature | range |
---|---|---|---|---|
66658 | positive | optimum | 30 | mesophilic |
66658 | positive | growth | 20-45 | |
67770 | positive | growth | 30 | mesophilic |
culture pH
@ref | ability | type | pH | PH range |
---|---|---|---|---|
66658 | positive | optimum | 7 | |
66658 | positive | growth | 5-10 | alkaliphile |
Physiology and metabolism
oxygen tolerance
- @ref: 66658
- oxygen tolerance: anaerobe
spore formation
- @ref: 66658
- type of spore: spore
- spore formation: yes
halophily
- @ref: 66658
- salt: NaCl
- growth: positive
- tested relation: growth
- concentration: 0-2 %
observation
- @ref: 66658
- observation: The cells utilize H2/CO2 , syngas (H2:CO2:CO=4:3:3), fructose, glycerol and other substances to generate acetate.
metabolite utilization
@ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
---|---|---|---|---|
66658 | 16024 | D-mannose | + | assimilation |
66658 | 17057 | cellobiose | + | assimilation |
66658 | 17306 | maltose | + | assimilation |
66658 | 17634 | D-glucose | + | assimilation |
66658 | 17992 | sucrose | + | assimilation |
66658 | 28260 | galactose | + | assimilation |
66658 | 29864 | mannitol | + | assimilation |
66658 | 61993 | maltotriose | + | assimilation |
66658 | 12936 | D-galactose | - | growth |
66658 | 15903 | beta-d-glucose | - | growth |
66658 | 16199 | urea | - | growth |
66658 | 16988 | D-ribose | - | growth |
66658 | 17203 | L-proline | - | growth |
66658 | 18186 | tyrosine | - | growth |
66658 | 25017 | leucine | - | growth |
66658 | 26020 | phosphate | - | growth |
66658 | 27442 | beta-D-fucose | - | growth |
66658 | 28044 | phenylalanine | - | growth |
66658 | 29016 | arginine | - | growth |
66658 | 30849 | L-arabinose | - | growth |
66658 | 42548 | alpha-L-fucose | - | growth |
66658 | 46987 | alpha-L-arabinopyranose | - | growth |
66658 | 59640 | N-acetylglucosamine | - | growth |
66658 | 65327 | D-xylose | - | growth |
66658 | 73617 | Phe-Ala-Pro | - | growth |
Isolation, sampling and environmental information
isolation
@ref | sample type | enrichment culture | enrichment culture composition | enrichment culture duration | isolation procedure |
---|---|---|---|---|---|
66658 | cow manure | DSMZ medium 311c | (per litre) has the following composition: 0.50 g NH4Cl, 0.50 g MgSO4.7H2O, 0.25 g CaCl2.2H2O, 2.25 g NaCl, 2.00 mg FeSO4.7H2O, 1.00 ml trace element solution SL-10 (DSMZ medium 320), 1.00 ml selenite-tungstate solution (see DSMZ medium 385), 2.00 g yeast extract, 2.00 g casitone, 0.50 ml Na-resazurin solution (0.1 % w/v), 0.35 g K2HPO4, 0.23 g KH2PO4, 4.00 g NaHCO3, 10.00 ml vitamin solution (see DSMZ medium 141), 0.30 g L-cysteine-HCl.H2O, 0.30 g Na2S.9 H2O, distilled water added to 1000 ml final volume. The pH was adjusted to 7.0 | 3 days | Hungate technique |
67770 | Cow manure |
isolation source categories
Cat1 | Cat2 | Cat3 |
---|---|---|
#Host | #Mammals | #Bovinae (Cow, Cattle) |
#Host Body Product | #Gastrointestinal tract | #Feces (Stool) |
taxonmaps
- @ref: 69479
- File name: preview.99_149025.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_34;96_3674;97_17920;98_98554;99_149025&stattab=map
- Last taxonomy: Firmicutes
- 16S sequence: MH346511
- Sequence Identity:
- Total samples: 1358
- soil counts: 491
- aquatic counts: 322
- animal counts: 307
- plant counts: 238
Sequence information
16S sequences
- @ref: 66658
- description: Clostridium sp. strain BXX 16S ribosomal RNA gene, partial sequence
- accession: MH346511
- length: 1502
- database: ena
- NCBI tax ID: 2184719
Genome sequences
@ref | description | accession | assembly level | database | NCBI tax ID |
---|---|---|---|---|---|
66792 | Clostridium bovifaecis BXX | GCA_009738435 | chromosome | ncbi | 2184719 |
66792 | Clostridium bovifaecis BXX | 2896726320 | complete | img | 2184719 |
GC content
@ref | GC-content | method |
---|---|---|
67770 | 32.6 | genome sequence analysis |
67770 | 33.7 | thermal denaturation, midpoint method (Tm) |
External links
@ref: 66658
culture collection no.: JCM 32382, CGMCC 1.5228
literature
- topic: Phylogeny
- Pubmed-ID: 30058994
- title: Clostridium bovifaecis sp. nov., a novel acetogenic bacterium isolated from cow manure.
- authors: Zhu H, Fu B, Lu S, Liu H, Liu H
- journal: Int J Syst Evol Microbiol
- DOI: 10.1099/ijsem.0.002928
- year: 2018
- mesh: Animals, Bacterial Typing Techniques, Base Composition, Cattle/*microbiology, Clostridium/*classification/genetics/isolation & purification, DNA, Bacterial/genetics, Fatty Acids/chemistry, Manure/*microbiology, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA
- topic2: Transcriptome
Reference
@id | authors | title | doi/url | journal | pubmed |
---|---|---|---|---|---|
20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | 10.1099/ijsem.0.004332 | ||
66658 | Hui Zhu, Bo Fu, Shuailing Lu, Hongbo Liu, He Liu | Clostridium bovifaecis sp. nov., a novel acetogenic bacterium isolated from cow manure | 10.1099/ijsem.0.002928 | IJSEM 68: 2956-2959 2018 | 30058994 |
66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | https://diaspora-project.de/progress.html#genomes | ||
67770 | Curators of the JCM | https://jcm.brc.riken.jp/en/ | |||
69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | MicrobeAtlas 1.0 beta | https://microbeatlas.org/ |