Strain identifier

BacDive ID: 159716

Type strain: Yes

Species: Actinophytocola glycyrrhizae

Strain history: CGMCC 4.7433 <-- J. Jiang; Jiangsu Normal Univ., China; BMP B8152.

NCBI tax ID(s): 2044873 (species)

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General

@ref: 66608

BacDive-ID: 159716

keywords: 16S sequence, Bacteria, aerobe, spore-forming, mesophilic, Gram-positive

description: Actinophytocola glycyrrhizae KCTC 49002 is an aerobe, spore-forming, mesophilic bacterium that was isolated from From soil.

NCBI tax id

  • NCBI tax id: 2044873
  • Matching level: species

strain history

@refhistory
67771<- Chengliang Cao, Jiangsu Normal Univ.
67770CGMCC 4.7433 <-- J. Jiang; Jiangsu Normal Univ., China; BMP B8152.

doi: 10.13145/bacdive159716.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Pseudonocardiales
  • family: Pseudonocardiaceae
  • genus: Actinophytocola
  • species: Actinophytocola glycyrrhizae
  • full scientific name: Actinophytocola glycyrrhizae Cao et al. 2018

@ref: 66608

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Pseudonocardiales

family: Pseudonocardiaceae

genus: Actinophytocola

species: Actinophytocola glycyrrhizae

type strain: yes

Morphology

cell morphology

  • @ref: 66608
  • gram stain: positive
  • motility: no

multicellular morphology

  • @ref: 66608
  • forms multicellular complex: yes
  • further description: Non-fragmented substrate mycelium develops well, and aerial mycelium fragments into rodshaped spores.

Culture and growth conditions

culture medium

@refnamegrowth
66608Czapek's agaryes
66608ISP 2yes
66608ISP 3yes
66608ISP 4yes
66608ISP 5yes
66608Nutrient agar (NA)yes
66608potato dextrose agaryes

culture temp

@refgrowthtypetemperaturerange
66608positiveoptimum28mesophilic
66608positivegrowth15-30
67770positivegrowth28mesophilic
67771positivegrowth28mesophilic

culture pH

@refabilitytypepHPH range
66608positiveoptimum7
66608positivegrowth5-9alkaliphile

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
66608aerobe
67771aerobe

spore formation

  • @ref: 66608
  • type of spore: spore
  • spore formation: yes

halophily

@refsaltgrowthtested relationconcentration
66608NaClpositivegrowth0-2 %(w/v)
66608NaClpositiveoptimum0-1 %(w/v)

observation

@refobservation
66608BMP B8152T grew well on ISP 3, PDA, nutrient agar and ISP 2 media, with yellowish white to gray yellow aerial mycelium, but weakly on ISP 4, ISP 5 and Czapek's agar, with light gray aerial mycelium
67770quinones: MK-9(H4)

metabolite utilization

@refmetaboliteutilization activitykind of utilization testedChebi-ID
66608milk-assimilation
66608D-galactose+assimilation12936
66608L-histidine+assimilation15971
66608D-mannose+assimilation16024
66608L-arginine+assimilation16467
66608L-methionine+assimilation16643
66608cellobiose+assimilation17057
66608L-serine+assimilation17115
66608L-asparagine+assimilation17196
66608L-sorbose+assimilation17266
66608L-tyrosine+assimilation17895
66608L-arabinose+assimilation30849
66608D-xylose+assimilation65327
66608gelatin+hydrolysis5291
66608urea+hydrolysis16199
66608starch+hydrolysis28017
66608casein+hydrolysis
66608nitrate+reduction17632

metabolite production

  • @ref: 66608
  • Chebi-ID: 16136
  • metabolite: hydrogen sulfide
  • production: no

enzymes

@refvalueactivityec
66608cytochrome oxidase-1.9.3.1
66608catalase+1.11.1.6

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinenthost specieslatitudelongitudeenrichment cultureenrichment culture compositionenrichment culture durationenrichment culture temperature
67771From soilXinjiangChinaCHNAsia
66608rhizosphere of Glycyrrhiza inflata collected ashoreKashi, Xinjiang provinceChinaCHNAsiaGlycyrrhiza inflata38.433375.0333ISP3supplemented with following antibiotics (l-1): 20 mg nalidixic acid, 10 mg cycloheximide and 10 mg nystatin.28 days28
67770Rhizosphere of Glycyrrhiza inflataKashi, Xinjiang Province, northwest ChinaChinaCHNAsiaGlycyrrhiza inflata

isolation source categories

Cat1Cat2Cat3
#Host#Plants#Herbaceous plants (Grass,Crops)
#Host Body-Site#Plant#Rhizosphere

Sequence information

16S sequences

  • @ref: 66608
  • description: Actinophytocola sp. BMP B8152 16S ribosomal RNA gene, partial sequence
  • accession: MG190031
  • length: 1513
  • database: ena
  • NCBI tax ID: 2044873

GC content

  • @ref: 66608
  • GC-content: 70.2
  • method: thermal denaturation, midpoint method (Tm)

External links

@ref: 66608

culture collection no.: KCTC 49002, CGMCC 4.7433, BMP B8152, JCM 33402

literature

  • topic: Phylogeny
  • Pubmed-ID: 29939121
  • title: Actinophytocola glycyrrhizae sp. nov. isolated from the rhizosphere of Glycyrrhiza inflata.
  • authors: Cao C, Sun Y, Wu B, Zhao S, Yuan B, Qin S, Jiang J, Huang Y
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijsem.0.002864
  • year: 2018
  • mesh: Actinomycetales/*classification/genetics/isolation & purification, Bacterial Typing Techniques, Base Composition, China, DNA, Bacterial/genetics, Diaminopimelic Acid/chemistry, Fatty Acids/chemistry, Glycyrrhiza/*microbiology, Nucleic Acid Hybridization, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, *Rhizosphere, Sequence Analysis, DNA, *Soil Microbiology, Vitamin K 2/analogs & derivatives/chemistry
  • topic2: Transcriptome

Reference

@idauthorstitledoi/urljournalpubmed
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
66608Chengliang Cao, Yong Sun, Bo Wu, Shuai Zhao, Bo Yuan, Sheng Qin, Jihong Jiang, Ying HuangActinophytocola glycyrrhizae sp. nov. isolated from the rhizosphere of Glycyrrhiza inflata10.1099/ijsem.0.002864IJSEM 68: 2504-2508 201829939121
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
67771Curators of the KCTChttps://kctc.kribb.re.kr/En/Kctc