Strain identifier

BacDive ID: 159702

Type strain: Yes

Species: Oceanisphaera avium

Strain Designation: AMac2203

Culture col. no.: KCTC 62118, JCM 32207

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General

@ref: 66567

BacDive-ID: 159702

keywords: 16S sequence, aerobe, gram-negative, motile, rod-shaped, colony-forming

description: Oceanisphaera avium AMac2203 is an aerobe, gram-negative, motile archaeon that forms circular colonies and was isolated from faecal samples from the gut of the cinereous vulture, Aegypius monachus.

doi: 10.13145/bacdive159702.20201210.5

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/proteobacteria
  • domain: Bacteria
  • phylum: Proteobacteria
  • class: Gammaproteobacteria
  • order: Aeromonadales
  • family: Aeromonadaceae
  • genus: Oceanisphaera
  • species: Oceanisphaera avium
  • full scientific name: Oceanisphaera avium Sung et al. 2018

@ref: 66567

order: Aeromonadales

family: Aeromonadaceae

genus: Oceanisphaera

species: Oceanisphaera avium

strain designation: AMac2203

type strain: yes

Morphology

cell morphology

  • @ref: 66567
  • gram stain: negative
  • cell shape: rod-shaped
  • motility: yes

colony morphology

  • @ref: 66567
  • colony color: beige
  • colony shape: circular
  • incubation period: 2 days
  • medium used: modified TYS agar

Culture and growth conditions

culture medium

  • @ref: 66567
  • name: modified TYS agar
  • growth: yes

culture temp

@refgrowthtypetemperature
66567positiveoptimum15-25
66567positivegrowth4-30

culture pH

@refabilitytypepH
66567positivegrowth6-9
66567positiveoptimum7-8

Physiology and metabolism

oxygen tolerance

  • @ref: 66567
  • oxygen tolerance: aerobe

halophily

@refsaltgrowthtested relationconcentration
66567NaClpositiveoptimum3-5 %(w/v)
66567NaClpositivegrowth0-8 %(w/v)

observation

  • @ref: 66567
  • observation: The OrthoANI value between strain AMac2203T and O. profunda KCTC 32510T is 77.5 %

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6656716523D-serine-assimilation
66567167632-oxobutanoate-assimilation
6656716865gamma-aminobutyric acid-assimilation
6656717203L-proline-assimilation
6656724741hydroxyproline-assimilation
6656727248urocanic acid-assimilation
6656728087glycogen-assimilation
66567370543-hydroxybutyrate-assimilation
6656753258sodium citrate-assimilation
6656753423tween 40-assimilation
6656753426tween 80-assimilation
66567645522-hydroxybutyrate-assimilation
6656773786L-alanylglycine-assimilation
6656775146monomethyl succinate-assimilation
66567143136succinamate-assimilation
66567168102-oxoglutarate-assimilation
6656715570D-alanine+assimilation
6656715603L-leucine+assimilation
6656715729L-ornithine+assimilation
6656715740formate+assimilation
6656715971L-histidine+assimilation
6656716411acetic acid+assimilation
6656716857L-threonine+assimilation
6656716977L-alanine+assimilation
6656717115L-serine+assimilation
6656717196L-asparagine+assimilation
6656717295L-phenylalanine+assimilation
6656721217L-alaninamide+assimilation
6656724996lactate+assimilation
6656725115malate+assimilation
6656729985L-glutamate+assimilation
6656729991L-aspartate+assimilation
6656730031succinate+assimilation
6656751850methyl pyruvate+assimilation
6656773706bromosuccinate+assimilation
6656716199urea-hydrolysis
665674853esculin+hydrolysis
6656717632nitrate-reduction

enzymes

@refvalueactivityec
66567DNase-
66567catalase+1.11.1.6
66567cytochrome oxidase+1.9.3.1
66567esterase (C 4)+
66567esterase Lipase (C 8)+
66567leucine arylamidase+3.4.11.1

Isolation, sampling and environmental information

isolation

  • @ref: 66567
  • sample type: faecal samples from the gut of the cinereous vulture, Aegypius monachus
  • geographic location: Seoul Grand Park Zoo
  • country: Republic of Korea
  • origin.country: KOR
  • continent: Asia
  • latitude: 37.4227
  • longitude: 127.022
  • isolation procedure: Faecal samples were diluted with filtered PBS and inoculated on Lactobacilli MRS agar (Difco), marine agar (Difco), MacConkey agar (BBL), Reasoner's 2A agar (Difco) and tryptic soy agar (Bacto) plates using the standard serial dilution plating method. Then, inoculated agar plates were incubated at 20 and 37°C. Finally, 126 isolates were picked and spread on fresh medium at least three times to obtain pure cultures

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
66567Oceanisphaera sp. strain AMac2203 16S ribosomal RNA gene, partial sequenceMH1746691559ena1903694
66567Oceanisphaera sp. strain AMac2203 16S ribosomal RNA gene, partial sequenceMH1746701547ena1903694
66567Oceanisphaera sp. strain AMac2203 16S ribosomal RNA gene, partial sequenceMH1746711546ena1903694
66567Oceanisphaera sp. strain AMac2203 16S ribosomal RNA gene, partial sequenceMH1746721559ena1903694

GC content

  • @ref: 66567
  • GC-content: 47.1
  • method: genome sequence analysis

External links

@ref: 66567

culture collection no.: KCTC 62118, JCM 32207

Reference

@idauthorstitledoi/urljournal
20215D.Gleim, M.Kracht, N.Weiss et. al.Prokaryotic Nomenclature Up-to-date - compilation of all names of Bacteria and Archaea, validly published according to the Bacteriological Code since 1. Jan. 1980, and validly published nomenclatural changes sincehttp://www.dsmz.de/bacterial-diversity/prokaryotic-nomenclature-up-to-date.html
66567Hojun Sung, Hyun Sik Kim, June-Young Lee, Woorim Kang, Pil Soo Kim, Dong-Wook Hyun, Euon Jung Tak, Mi-Ja Jung, Ji-Hyun Yun, Min-Soo Kim, Na-Ri Shin, Tae Woong Whon, Jeong Rae Rho, Sun Duk Park, Hyung Eun Shim, Jin-Woo BaeOceanisphaera avium sp. nov., isolated from the gut of the cinereous vulture, Aegypius monachus10.1099/ijsem.0.002797IJSEM 68: 2068-2073 2018