Strain identifier

BacDive ID: 159644

Type strain: Yes

Species: Clostridium beihaiense

Strain Designation: YB-7

Strain history: <- CICC

NCBI tax ID(s): 2282766 (species)

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General

@ref: 66494

BacDive-ID: 159644

DSM-Number: 110900

keywords: 16S sequence, Bacteria, anaerobe, spore-forming, mesophilic, Gram-positive, rod-shaped

description: Clostridium beihaiense YB-7 is an anaerobe, spore-forming, mesophilic bacterium that was isolated from From activated sludge of an anaerobic baffled reactor pond.

NCBI tax id

  • NCBI tax id: 2282766
  • Matching level: species

strain history

@refhistory
66494<- KCTC; KCTC 15555 <- CICC; CICC 24109
67771<- CICC

doi: 10.13145/bacdive159644.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Clostridia
  • order: Eubacteriales
  • family: Clostridiaceae
  • genus: Clostridium
  • species: Clostridium beihaiense
  • full scientific name: Clostridium beihaiense Dong et al. 2018

@ref: 66494

domain: Bacteria

phylum: Firmicutes

class: Clostridia

order: Eubacteriales

family: Clostridiaceae

genus: Clostridium

species: Clostridium beihaiense

full scientific name: Clostridium beihaiense Dong et al. 2018

strain designation: YB-7

type strain: yes

Morphology

cell morphology

@refgram staincell lengthcell widthcell shape
66625positive2.4-5.7 µm0.9-1 µmrod-shaped
67771rod-shaped
67771positive

Culture and growth conditions

culture medium

  • @ref: 66494
  • name: PY + X MEDIUM (N2/CO2) (DSMZ Medium 104c)
  • growth: yes
  • link: https://mediadive.dsmz.de/medium/104c
  • composition: Name: PY + X MEDIUM (N2/CO2) (DSMZ Medium 104c) Composition: Yeast extract 10.0 g/l D-Glucose 5.0 g/l Trypticase peptone 5.0 g/l Meat peptone 5.0 g/l Na2CO3 1.0 g/l L-Cysteine HCl x H2O 0.5 g/l NaHCO3 0.4 g/l NaCl 0.08 g/l KH2PO4 0.04 g/l K2HPO4 0.04 g/l MgSO4 x 7 H2O 0.02 g/l CaCl2 x 2 H2O 0.01 g/l Sodium resazurin 0.0005 g/l Distilled water

culture temp

@refgrowthtypetemperaturerange
66494positivegrowth37mesophilic
66625positiveoptimum37mesophilic
66625positivegrowth20-45
67771positivegrowth37mesophilic

culture pH

@refabilitytypepHPH range
66625positiveoptimum7
66625positivegrowth5-12alkaliphile

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
66625anaerobe
67771anaerobe

spore formation

  • @ref: 66625
  • type of spore: spore
  • spore formation: yes

halophily

@refsaltgrowthtested relationconcentration
66625NaClpositivegrowth0-5 %(w/v)
66625NaClpositiveoptimum5 %(w/v)

observation

  • @ref: 66625
  • observation: cells are observed as single cells, pairs, or chains of cells arranged on the plate after culturing 72 h

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
666256731melezitose-assimilation
6662515903beta-d-glucose-assimilation
6662516634raffinose-assimilation
6662517057cellobiose-assimilation
6662517234glucose-assimilation
6662517306maltose-assimilation
6662517716lactose-assimilation
6662517754glycerol-assimilation
6662517814salicin-assimilation
6662517992sucrose-assimilation
6662518222xylose-assimilation
6662522599arabinose-assimilation
6662526546rhamnose-assimilation
6662527082trehalose-assimilation
6662527252uronic acid-assimilation
6662529864mannitol-assimilation
6662530911sorbitol-assimilation
6662535581indole-assimilation
6662528017starch-hydrolysis
666255291gelatin+hydrolysis
6662517632nitrate-reduction

enzymes

@refvalueactivityec
66625alpha-galactosidase-3.2.1.22
66625beta-D-fucosidase-3.2.1.38
66625beta-galactosidase-3.2.1.23
66625beta-glucosidase-3.2.1.21
66625catalase-1.11.1.6
66625chymotrypsin-3.4.4.5
66625cystine arylamidase-3.4.11.3
66625cytochrome oxidase-1.9.3.1
66625esterase Lipase (C 8)-
66625leucine arylamidase-3.4.11.1
66625lipase (C 14)-
66625N-acetyl-beta-glucosaminidase-3.2.1.52
66625valine arylamidase-
66625acid phosphatase+3.1.3.2
66625alkaline phosphatase+3.1.3.1
66625alpha-glucosidase+3.2.1.20
66625esterase (C 4)+
66625naphthol-AS-BI-phosphohydrolase+
66625trypsin+3.4.21.4
66625urease+3.5.1.5

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentlatitudelongitudeenrichment cultureenrichment culture compositionenrichment culture durationenrichment culture temperatureisolation procedure
67771From activated sludge of an anaerobic baffled reactor(ABR) pondBeihai, GuangxiChinaCHNAsia21.0428109.088
66494active sludge of an anaerobic baffled reactor in wastewater treatment plantGuangxi, BeihaiChinaCHNAsia
66625activated sludge of WTWTPBeihai, GuangxiChinaCHNAsia21.0427109.088Reinforced Clostridial Agar10.0 g l-1 peptone, 10.0 g l-1 beef extract, 3.0 g l-1 yeast extract, 5.0 g l-1 glucose, 1.0 g l-1 soluble starch, 5.0 g l-1 NaCl, 5.0 g l-1 sodium acetate, 0.5 g l-1 L-cysteine and 1.5 % agar7 days37The sample was diluted to 10-2 times and plated on Reinforced Clostridial Agar at 37°C for 7 days

isolation source categories

Cat1Cat2Cat3
#Engineered#Waste#Activated sludge
#Engineered#Waste#Water treatment plant
#Condition#Anoxic (anaerobic)

Sequence information

16S sequences

  • @ref: 66625
  • description: Clostridium sp. strain ABR-7 16S ribosomal RNA gene, partial sequence
  • accession: MF125275
  • length: 1382
  • database: ena
  • NCBI tax ID: 2282766

GC content

@refGC-contentmethod
6649432.3high performance liquid chromatography (HPLC)
6777132.3

External links

@ref: 66494

culture collection no.: DSM 110900, CICC 24109, KCTC 15555

straininfo link

  • @ref: 112118
  • straininfo: 407080

literature

  • topic: Phylogeny
  • Pubmed-ID: 30028283
  • title: Clostridium beihaiense sp. nov., an anaerobic bacterium isolated from activated sludge.
  • authors: Dong Y, Liu Y, Chen N, Zhong Y, Liu L, Xie Q
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijsem.0.002885
  • year: 2018
  • mesh: Bacterial Typing Techniques, Base Composition, China, Clostridium/*classification/genetics/isolation & purification, DNA, Bacterial/genetics, Fatty Acids/chemistry, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Sewage/*microbiology, Waste Water/microbiology
  • topic2: Transcriptome

Reference

@idauthorstitledoi/urlcataloguejournalpubmed
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
66494Curators of the DSMZhttps://www.dsmz.de/collection/catalogue/details/culture/DSM-110900Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 110900)
66625Yingying Dong, Yuhui Liu, Nanchun Chen, Yijian Zhong, Li Liu, Qinglin XieClostridium beihaiense sp. nov., an anaerobic bacterium isolated from activated sludge10.1099/ijsem.0.002885IJSEM 68: 2789-2793 201830028283
67771Curators of the KCTChttps://kctc.kribb.re.kr/En/Kctc
112118Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.StrainInfo: A central database for resolving microbial strain identifiers10.60712/SI-ID407080.1