Strain identifier
BacDive ID: 159629
Type strain:
Species: Streptomyces altiplanensis
Strain Designation: HST21
Strain history: <- I. Nouioui, Univ. Newcastle, Newcastle upon Tyne, UK; HST21 <- C. Cortes, Universidad de Chile; HST21
NCBI tax ID(s): 1854571 (species)
General
@ref: 66465
BacDive-ID: 159629
DSM-Number: 107267
keywords: genome sequence, 16S sequence, Bacteria, aerobe, spore-forming, mesophilic
description: Streptomyces altiplanensis HST21 is an aerobe, spore-forming, mesophilic bacterium that builds an aerial mycelium and was isolated from soil.
NCBI tax id
- NCBI tax id: 1854571
- Matching level: species
strain history
- @ref: 66465
- history: <- I. Nouioui, Univ. Newcastle, Newcastle upon Tyne, UK; HST21 <- C. Cortes, Universidad de Chile; HST21
doi: 10.13145/bacdive159629.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/actinomycetota
- domain: Bacteria
- phylum: Actinomycetota
- class: Actinomycetes
- order: Kitasatosporales
- family: Streptomycetaceae
- genus: Streptomyces
- species: Streptomyces altiplanensis
- full scientific name: Streptomyces altiplanensis Cortés-Albayay et al. 2019
@ref: 66465
domain: Bacteria
phylum: Actinobacteria
class: Actinobacteria
order: Streptomycetales
family: Streptomycetaceae
genus: Streptomyces
species: Streptomyces altiplanensis
full scientific name: Streptomyces altiplanensis Cortés-Albayay et al. 2019
strain designation: HST21
type strain: yes
Morphology
colony morphology
@ref | colony color | medium used |
---|---|---|
69237 | Chocolate brown (8017) | suter with tyrosine |
69237 | Black brown (8022), olive brown (8008) | ISP 7 |
69237 | Black brown (8022) | suter without tyrosine |
69237 | Beige (1001) | ISP 2 |
69237 | Mahogany brown (8016) | ISP 4 |
69237 | Mahogany brown (8016) | ISP 5 |
69237 | Nut brown (8011), agate grey (7038) | ISP 3 |
multicellular morphology
@ref | forms multicellular complex | complex name | medium name | complex color |
---|---|---|---|---|
69237 | no | Aerial mycelium | ISP 2 | |
69237 | yes | Aerial mycelium | ISP 3 | Grey white (9002) |
69237 | yes | Aerial mycelium | ISP 4 | Cream (9001) |
69237 | no | Aerial mycelium | ISP 5 | |
69237 | no | Aerial mycelium | ISP 7 | |
69237 | no | Aerial mycelium | suter with tyrosine | |
69237 | no | Aerial mycelium | suter without tyrosine |
pigmentation
@ref | production | name | color |
---|---|---|---|
67995 | yes | diffusible pigments | |
69237 | no | Melanin | |
69237 | yes | soluble pigment | Copper brown (8004), signal brown (8002), ochre brown (8001), clay brown (8003), chestnut brown (8015) |
Culture and growth conditions
culture medium
- @ref: 66465
- name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)
- growth: yes
- link: https://mediadive.dsmz.de/medium/65
- composition: Name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) Composition: Agar 18.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water
culture temp
@ref | growth | type | temperature | range |
---|---|---|---|---|
66465 | positive | growth | 28 | mesophilic |
67995 | positive | growth | 28-37 | mesophilic |
67995 | positive | minimum | 25 | mesophilic |
67995 | positive | maximum | 45 | thermophilic |
culture pH
- @ref: 67995
- ability: positive
- type: optimum
- pH: 9.0-11.0
- PH range: alkaliphile
Physiology and metabolism
oxygen tolerance
- @ref: 67995
- oxygen tolerance: aerobe
spore formation
- @ref: 67995
- spore description: rectiflexible spore chains in section with spiny spore surfaces
- spore formation: yes
halophily
- @ref: 69237
- salt: NaCl
- growth: positive
- tested relation: growth
- concentration: 0-2.5 %
observation
@ref | observation |
---|---|
67995 | Whole-cell hydrolysates are rich in LL-diaminopimelic acid in its peptidoglycan and glucose and ribose in its cell-wall sugars |
67995 | It is characterized by the presence of DPG, PME, PE, PI, GPLs and unknown lipids and phospholipids as polar lipids |
67995 | The menaquinone profile contained MK-9 (H6), MK-9(H8), MK-9(H4), MK-7(H2), MK-8(H2), MK-9(H2) and MK-10 |
67995 | On ISP 1 Substrate mycelium colour: Deep reddish brown, Aerial mycelium colour: dark brown, diffusible pigments: strong reddish brown |
67995 | On ISP 2 Substrate mycelium colour: white , Aerial mycelium colour: white, diffusible pigments: no |
67995 | On ISP 3 Aerial mycelium colour: white, diffusible pigments: deep reddish brown |
67995 | On ISP 4 Substrate mycelium colour: dark greyish yellow, Aerial mycelium colour: light greyish olive, diffusible pigments: vivid greenish yellow |
67995 | On ISP 5 Substrate mycelium colour: light olive brown, Aerial mycelium colour: strong yellowish brown, diffusible pigments: strong yellow |
67995 | On ISP 7 Substrate mycelium colour: dark yellowish brown, Aerial mycelium colour: light olive brown, diffusible pigments: dark yellow |
67995 | On GYM Substrate mycelium colour: strong brown, Aerial mycelium colour: white, diffusible pigments: deep reddish brown |
metabolite utilization
@ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
---|---|---|---|---|
69237 | 22599 | arabinose | +/- | growth |
67995 | 17992 | sucrose | + | carbon source |
67995 | 17164 | stachyose | + | carbon source |
67995 | 16634 | raffinose | + | carbon source |
67995 | 15824 | D-fructose | + | carbon source |
67995 | 12936 | D-galactose | + | carbon source |
67995 | 18183 | L-pyroglutamic acid | + | assimilation |
67995 | 17521 | (-)-quinic acid | + | assimilation |
67995 | 8295 | beta-hydroxybutyrate | + | assimilation |
67995 | 64552 | alpha-hydroxybutyrate | + | assimilation |
67995 | 17968 | butyrate | + | assimilation |
67995 | 16467 | L-arginine | + | nitrogen source |
67995 | 161680 | aztreonam | + | growth |
67995 | 48607 | lithium chloride | + | growth |
67995 | 53423 | tween 40 | + | growth |
67995 | 75229 | sodium bromate | + | growth |
67995 | 1 % sodium lactate | + | growth | |
69237 | 62968 | cellulose | - | growth |
69237 | 28757 | fructose | + | growth |
69237 | 17234 | glucose | + | growth |
69237 | 17268 | inositol | - | growth |
69237 | 37684 | mannose | +/- | growth |
69237 | 16634 | raffinose | + | growth |
69237 | 26546 | rhamnose | +/- | growth |
69237 | 17992 | sucrose | + | growth |
69237 | 18222 | xylose | - | growth |
68379 | 17632 | nitrate | - | reduction |
68379 | 4853 | esculin | - | hydrolysis |
68379 | 16199 | urea | - | hydrolysis |
68379 | 5291 | gelatin | + | hydrolysis |
68379 | 17634 | D-glucose | - | fermentation |
68379 | 16988 | D-ribose | - | fermentation |
68379 | 65327 | D-xylose | - | fermentation |
68379 | 16899 | D-mannitol | - | fermentation |
68379 | 17306 | maltose | - | fermentation |
68379 | 17716 | lactose | - | fermentation |
68379 | 17992 | sucrose | - | fermentation |
68379 | 28087 | glycogen | - | fermentation |
enzymes
@ref | value | activity | ec |
---|---|---|---|
68379 | gelatinase | + | |
68379 | urease | - | 3.5.1.5 |
68379 | beta-glucosidase | - | 3.2.1.21 |
68379 | N-acetyl-beta-glucosaminidase | + | 3.2.1.52 |
68379 | alpha-glucosidase | + | 3.2.1.20 |
68379 | beta-galactosidase | + | 3.2.1.23 |
68379 | beta-glucuronidase | - | 3.2.1.31 |
68379 | alkaline phosphatase | + | 3.1.3.1 |
68379 | pyrrolidonyl arylamidase | - | 3.4.19.3 |
68379 | pyrazinamidase | - | 3.5.1.B15 |
68382 | alkaline phosphatase | + | 3.1.3.1 |
68382 | esterase (C 4) | + | |
68382 | esterase lipase (C 8) | + | |
68382 | leucine arylamidase | + | 3.4.11.1 |
68382 | cystine arylamidase | - | 3.4.11.3 |
68382 | trypsin | - | 3.4.21.4 |
68382 | alpha-chymotrypsin | - | 3.4.21.1 |
68382 | naphthol-AS-BI-phosphohydrolase | + | |
68382 | alpha-galactosidase | - | 3.2.1.22 |
68382 | beta-galactosidase | + | 3.2.1.23 |
68382 | beta-glucuronidase | - | 3.2.1.31 |
68382 | N-acetyl-beta-glucosaminidase | + | 3.2.1.52 |
68382 | alpha-mannosidase | - | 3.2.1.24 |
68382 | alpha-fucosidase | - | 3.2.1.51 |
API coryne
@ref | NIT | PYZ | PYRA | PAL | beta GUR | beta GAL | alpha GLU | beta NAG | ESC | URE | GEL | GLU | RIB | XYL | MAN | MAL | LAC | SAC | GLYG |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
69237 | - | - | - | + | - | + | + | + | - | - | + | - | - | - | - | - | - | - | - |
API zym
@ref | Alkaline phosphatase | Esterase | Esterase Lipase | Lipase | Leucine arylamidase | Valine arylamidase | Cystine arylamidase | Trypsin | alpha- Chymotrypsin | Acid phosphatase | Naphthol-AS-BI-phosphohydrolase | alpha- Galactosidase | beta- Galactosidase | beta- Glucuronidase | alpha- Glucosidase | beta- Glucosidase | N-acetyl-beta- glucosaminidase | alpha- Mannosidase | alpha- Fucosidase |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
69237 | + | + | + | +/- | + | +/- | - | - | - | +/- | + | - | + | - | +/- | +/- | + | - | - |
Isolation, sampling and environmental information
isolation
@ref | sample type | geographic location | country | origin.country | continent | latitude | longitude |
---|---|---|---|---|---|---|---|
66465 | soil | Tarapaca Region, Salar de Huasco (20° 19' 43'' N, 68° 50' 19'' W) | Chile | CHL | Middle and South America | 20.3286 | -68.8386 |
67995 | hyper arid soil of the Salar de Huasco, (3800 m.a.s.l) | Atacama Desert | Chile | CHL | Middle and South America |
isolation source categories
- Cat1: #Environmental
- Cat2: #Terrestrial
- Cat3: #Soil
Sequence information
16S sequences
- @ref: 66465
- description: Streptomyces altiplanensis strain HST21 16S ribosomal RNA gene, complete sequence
- accession: KX130868.2
- length: 1530
- database: ena
- NCBI tax ID: 1854571
Genome sequences
- @ref: 67995
- description: Streptomyces altiplanensis HST21
- accession: GCA_003719395
- assembly level: contig
- database: ncbi
- NCBI tax ID: 1854571
GC content
@ref | GC-content | method |
---|---|---|
66465 | 71.0 | sequence analysis |
67995 | 71 | genome sequence analysis |
Genome-based predictions
predictions
trait | prediction | confidence | training_data |
---|---|---|---|
motile | no | 91.809 | no |
flagellated | no | 98.118 | no |
gram-positive | yes | 89.681 | no |
anaerobic | no | 99.294 | yes |
aerobic | yes | 94.903 | yes |
halophile | no | 91.426 | no |
spore-forming | yes | 93.806 | yes |
thermophile | no | 97.657 | no |
glucose-util | yes | 89.97 | no |
glucose-ferment | no | 88.225 | no |
External links
@ref: 66465
culture collection no.: DSM 107267, CECT 9647
straininfo link
- @ref: 112103
- straininfo: 398509
literature
- topic: Phylogeny
- Pubmed-ID: 31204968
- title: Streptomyces altiplanensis sp. nov., an alkalitolerant species isolated from Chilean Altiplano soil, and emended description of Streptomyces chryseus (Krasil'nikov et al. 1965) Pridham 1970.
- authors: Cortes-Albayay C, Dorador C, Schumann P, Schniete JK, Herron P, Andrews B, Asenjo J, Nouioui I
- journal: Int J Syst Evol Microbiol
- DOI: 10.1099/ijsem.0.003525
- year: 2019
- mesh: Alkalies, Altitude, Bacterial Typing Techniques, Base Composition, Chile, DNA, Bacterial/genetics, *Desert Climate, Diaminopimelic Acid/chemistry, Fatty Acids/chemistry, Hydrogen-Ion Concentration, Nucleic Acid Hybridization, Peptidoglycan/chemistry, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, *Soil Microbiology, Streptomyces/*classification/isolation & purification
- topic2: Transcriptome
Reference
@id | authors | title | doi/url | catalogue | journal |
---|---|---|---|---|---|
20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | 10.1099/ijsem.0.004332 | ||
66465 | Curators of the DSMZ | https://www.dsmz.de/collection/catalogue/details/culture/DSM-107267 | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 107267) | ||
67995 | Carlos Cortés-Albayay, Cristina Dorador, Peter Schumann, Jana K. Schniete, Paul Herron, Barbara Andrews, Juan Asenjo, Imen Nouioui | Streptomyces altiplanensis sp. nov., an alkalitolerant species isolated from Chilean Altiplano soil, and emended description of Streptomyces chryseus (Krasil'nikov et al. 1965) Pridham 1970 | 10.1099/ijsem.0.003525 | IJSEM 69: 2498-2505 2019 | |
68379 | Automatically annotated from API Coryne | ||||
68382 | Automatically annotated from API zym | ||||
69237 | Wink, J. | Compendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig | https://cdn.dsmz.de/wink/DSM%20107267.pdf | ||
112103 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | StrainInfo: A central database for resolving microbial strain identifiers | 10.60712/SI-ID398509.1 |