Strain identifier

BacDive ID: 159629

Type strain: Yes

Species: Streptomyces altiplanensis

Strain Designation: HST21

Strain history: <- I. Nouioui, Univ. Newcastle, Newcastle upon Tyne, UK; HST21 <- C. Cortes, Universidad de Chile; HST21

NCBI tax ID(s): 1854571 (species)

For citation purpose refer to the digital object identifier (doi) of the current version.
Archive
version 8.1 (current version):
version 8:
version 7.1:
version 7:
version 6:
version 5:
version 8.1 (current version)

General

@ref: 66465

BacDive-ID: 159629

DSM-Number: 107267

keywords: genome sequence, 16S sequence, Bacteria, aerobe, spore-forming, mesophilic

description: Streptomyces altiplanensis HST21 is an aerobe, spore-forming, mesophilic bacterium that builds an aerial mycelium and was isolated from soil.

NCBI tax id

  • NCBI tax id: 1854571
  • Matching level: species

strain history

  • @ref: 66465
  • history: <- I. Nouioui, Univ. Newcastle, Newcastle upon Tyne, UK; HST21 <- C. Cortes, Universidad de Chile; HST21

doi: 10.13145/bacdive159629.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Kitasatosporales
  • family: Streptomycetaceae
  • genus: Streptomyces
  • species: Streptomyces altiplanensis
  • full scientific name: Streptomyces altiplanensis Cortés-Albayay et al. 2019

@ref: 66465

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Streptomycetales

family: Streptomycetaceae

genus: Streptomyces

species: Streptomyces altiplanensis

full scientific name: Streptomyces altiplanensis Cortés-Albayay et al. 2019

strain designation: HST21

type strain: yes

Morphology

colony morphology

@refcolony colormedium used
69237Chocolate brown (8017)suter with tyrosine
69237Black brown (8022), olive brown (8008)ISP 7
69237Black brown (8022)suter without tyrosine
69237Beige (1001)ISP 2
69237Mahogany brown (8016)ISP 4
69237Mahogany brown (8016)ISP 5
69237Nut brown (8011), agate grey (7038)ISP 3

multicellular morphology

@refforms multicellular complexcomplex namemedium namecomplex color
69237noAerial myceliumISP 2
69237yesAerial myceliumISP 3Grey white (9002)
69237yesAerial myceliumISP 4Cream (9001)
69237noAerial myceliumISP 5
69237noAerial myceliumISP 7
69237noAerial myceliumsuter with tyrosine
69237noAerial myceliumsuter without tyrosine

pigmentation

@refproductionnamecolor
67995yesdiffusible pigments
69237noMelanin
69237yessoluble pigmentCopper brown (8004), signal brown (8002), ochre brown (8001), clay brown (8003), chestnut brown (8015)

Culture and growth conditions

culture medium

  • @ref: 66465
  • name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)
  • growth: yes
  • link: https://mediadive.dsmz.de/medium/65
  • composition: Name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) Composition: Agar 18.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water

culture temp

@refgrowthtypetemperaturerange
66465positivegrowth28mesophilic
67995positivegrowth28-37mesophilic
67995positiveminimum25mesophilic
67995positivemaximum45thermophilic

culture pH

  • @ref: 67995
  • ability: positive
  • type: optimum
  • pH: 9.0-11.0
  • PH range: alkaliphile

Physiology and metabolism

oxygen tolerance

  • @ref: 67995
  • oxygen tolerance: aerobe

spore formation

  • @ref: 67995
  • spore description: rectiflexible spore chains in section with spiny spore surfaces
  • spore formation: yes

halophily

  • @ref: 69237
  • salt: NaCl
  • growth: positive
  • tested relation: growth
  • concentration: 0-2.5 %

observation

@refobservation
67995Whole-cell hydrolysates are rich in LL-diaminopimelic acid in its peptidoglycan and glucose and ribose in its cell-wall sugars
67995It is characterized by the presence of DPG, PME, PE, PI, GPLs and unknown lipids and phospholipids as polar lipids
67995The menaquinone profile contained MK-9 (H6), MK-9(H8), MK-9(H4), MK-7(H2), MK-8(H2), MK-9(H2) and MK-10
67995On ISP 1 Substrate mycelium colour: Deep reddish brown, Aerial mycelium colour: dark brown, diffusible pigments: strong reddish brown
67995On ISP 2 Substrate mycelium colour: white , Aerial mycelium colour: white, diffusible pigments: no
67995On ISP 3 Aerial mycelium colour: white, diffusible pigments: deep reddish brown
67995On ISP 4 Substrate mycelium colour: dark greyish yellow, Aerial mycelium colour: light greyish olive, diffusible pigments: vivid greenish yellow
67995On ISP 5 Substrate mycelium colour: light olive brown, Aerial mycelium colour: strong yellowish brown, diffusible pigments: strong yellow
67995On ISP 7 Substrate mycelium colour: dark yellowish brown, Aerial mycelium colour: light olive brown, diffusible pigments: dark yellow
67995On GYM Substrate mycelium colour: strong brown, Aerial mycelium colour: white, diffusible pigments: deep reddish brown

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6923722599arabinose+/-growth
6799517992sucrose+carbon source
6799517164stachyose+carbon source
6799516634raffinose+carbon source
6799515824D-fructose+carbon source
6799512936D-galactose+carbon source
6799518183L-pyroglutamic acid+assimilation
6799517521(-)-quinic acid+assimilation
679958295beta-hydroxybutyrate+assimilation
6799564552alpha-hydroxybutyrate+assimilation
6799517968butyrate+assimilation
6799516467L-arginine+nitrogen source
67995161680aztreonam+growth
6799548607lithium chloride+growth
6799553423tween 40+growth
6799575229sodium bromate+growth
679951 % sodium lactate+growth
6923762968cellulose-growth
6923728757fructose+growth
6923717234glucose+growth
6923717268inositol-growth
6923737684mannose+/-growth
6923716634raffinose+growth
6923726546rhamnose+/-growth
6923717992sucrose+growth
6923718222xylose-growth
6837917632nitrate-reduction
683794853esculin-hydrolysis
6837916199urea-hydrolysis
683795291gelatin+hydrolysis
6837917634D-glucose-fermentation
6837916988D-ribose-fermentation
6837965327D-xylose-fermentation
6837916899D-mannitol-fermentation
6837917306maltose-fermentation
6837917716lactose-fermentation
6837917992sucrose-fermentation
6837928087glycogen-fermentation

enzymes

@refvalueactivityec
68379gelatinase+
68379urease-3.5.1.5
68379beta-glucosidase-3.2.1.21
68379N-acetyl-beta-glucosaminidase+3.2.1.52
68379alpha-glucosidase+3.2.1.20
68379beta-galactosidase+3.2.1.23
68379beta-glucuronidase-3.2.1.31
68379alkaline phosphatase+3.1.3.1
68379pyrrolidonyl arylamidase-3.4.19.3
68379pyrazinamidase-3.5.1.B15
68382alkaline phosphatase+3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)+
68382leucine arylamidase+3.4.11.1
68382cystine arylamidase-3.4.11.3
68382trypsin-3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase+3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382N-acetyl-beta-glucosaminidase+3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API coryne

@refNITPYZPYRAPALbeta GURbeta GALalpha GLUbeta NAGESCUREGELGLURIBXYLMANMALLACSACGLYG
69237---+-+++--+--------

API zym

@refAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
69237++++/-++/----+/-+-+-+/-+/-+--

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentlatitudelongitude
66465soilTarapaca Region, Salar de Huasco (20° 19' 43'' N, 68° 50' 19'' W)ChileCHLMiddle and South America20.3286-68.8386
67995hyper arid soil of the Salar de Huasco, (3800 m.a.s.l)Atacama DesertChileCHLMiddle and South America

isolation source categories

  • Cat1: #Environmental
  • Cat2: #Terrestrial
  • Cat3: #Soil

Sequence information

16S sequences

  • @ref: 66465
  • description: Streptomyces altiplanensis strain HST21 16S ribosomal RNA gene, complete sequence
  • accession: KX130868.2
  • length: 1530
  • database: ena
  • NCBI tax ID: 1854571

Genome sequences

  • @ref: 67995
  • description: Streptomyces altiplanensis HST21
  • accession: GCA_003719395
  • assembly level: contig
  • database: ncbi
  • NCBI tax ID: 1854571

GC content

@refGC-contentmethod
6646571.0sequence analysis
6799571genome sequence analysis

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
motileno91.809no
flagellatedno98.118no
gram-positiveyes89.681no
anaerobicno99.294yes
aerobicyes94.903yes
halophileno91.426no
spore-formingyes93.806yes
thermophileno97.657no
glucose-utilyes89.97no
glucose-fermentno88.225no

External links

@ref: 66465

culture collection no.: DSM 107267, CECT 9647

straininfo link

  • @ref: 112103
  • straininfo: 398509

literature

  • topic: Phylogeny
  • Pubmed-ID: 31204968
  • title: Streptomyces altiplanensis sp. nov., an alkalitolerant species isolated from Chilean Altiplano soil, and emended description of Streptomyces chryseus (Krasil'nikov et al. 1965) Pridham 1970.
  • authors: Cortes-Albayay C, Dorador C, Schumann P, Schniete JK, Herron P, Andrews B, Asenjo J, Nouioui I
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijsem.0.003525
  • year: 2019
  • mesh: Alkalies, Altitude, Bacterial Typing Techniques, Base Composition, Chile, DNA, Bacterial/genetics, *Desert Climate, Diaminopimelic Acid/chemistry, Fatty Acids/chemistry, Hydrogen-Ion Concentration, Nucleic Acid Hybridization, Peptidoglycan/chemistry, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, *Soil Microbiology, Streptomyces/*classification/isolation & purification
  • topic2: Transcriptome

Reference

@idauthorstitledoi/urlcataloguejournal
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
66465Curators of the DSMZhttps://www.dsmz.de/collection/catalogue/details/culture/DSM-107267Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 107267)
67995Carlos Cortés-Albayay, Cristina Dorador, Peter Schumann, Jana K. Schniete, Paul Herron, Barbara Andrews, Juan Asenjo, Imen NouiouiStreptomyces altiplanensis sp. nov., an alkalitolerant species isolated from Chilean Altiplano soil, and emended description of Streptomyces chryseus (Krasil'nikov et al. 1965) Pridham 197010.1099/ijsem.0.003525IJSEM 69: 2498-2505 2019
68379Automatically annotated from API Coryne
68382Automatically annotated from API zym
69237Wink, J.Compendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweighttps://cdn.dsmz.de/wink/DSM%20107267.pdf
112103Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.StrainInfo: A central database for resolving microbial strain identifiers10.60712/SI-ID398509.1