Strain identifier

BacDive ID: 159628

Type strain: Yes

Species: Suipraeoptans intestinalis

Strain Designation: 68-1-5

Strain history: B. Abt; DSMZ, Germany; DSM 104945 (=68-1-5) <-- T. Looft; ARS, USDA, USA.

NCBI tax ID(s): 2606628 (species)

For citation purpose refer to the digital object identifier (doi) of the current version.
Archive
version 8.1 (current version):
version 8:
version 7.1:
version 7:
version 6:
version 5:
version 8.1 (current version)

General

@ref: 66464

BacDive-ID: 159628

DSM-Number: 104945

keywords: genome sequence, 16S sequence, Bacteria, anaerobe, mesophilic

description: Suipraeoptans intestinalis 68-1-5 is an anaerobe, mesophilic bacterium that was isolated from intestinal tract of a three month-old healthy pig.

NCBI tax id

  • NCBI tax id: 2606628
  • Matching level: species

strain history

@refhistory
66464<- T. Looft, USDA-ARS-NADC; 68-1-5
67770B. Abt; DSMZ, Germany; DSM 104945 (=68-1-5) <-- T. Looft; ARS, USDA, USA.

doi: 10.13145/bacdive159628.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Clostridia
  • order: Eubacteriales
  • family: Lachnospiraceae
  • genus: Suipraeoptans
  • species: Suipraeoptans intestinalis
  • full scientific name: Suipraeoptans intestinalis corrig. Wylensek et al. 2021
  • synonyms

    • @ref: 20215
    • synonym: Suipraoptans intestinalis

@ref: 66464

domain: Bacteria

phylum: Firmicutes

class: Clostridia

order: Eubacteriales

family: Lachnospiraceae

genus: Suipraeoptans

species: Suipraeoptans intestinalis

full scientific name: Suipraeoptans intestinalis

strain designation: 68-1-5

type strain: yes

Morphology

cell morphology

@refmotilityconfidencegram staincell lengthcell shape
69480no91.181
69480100positive
69645positive0.5 µmrod-shaped

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
66464BHI MEDIUM (DSMZ Medium 215)yeshttps://mediadive.dsmz.de/medium/215Name: BHI MEDIUM (DSMZ Medium 215) Composition: Brain heart infusion 37.0 g/l Distilled water
66464COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)yeshttps://mediadive.dsmz.de/medium/693Name: COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) Composition: Defibrinated sheep blood 50.0 g/l Columbia agar base
69645Hog gastric mucin mediumyes
69645Brain Heart Infusion (Oxoid, ref. CM1135)yes
69645Wilkins-Chalgren Anaerobe brothyessupplemented with L-cysteine and DTT (0.05% and 0.02% (w/v), respectively)

culture temp

@refgrowthtypetemperaturerange
66464positivegrowth37mesophilic
67770positivegrowth37mesophilic
69645positivegrowth37mesophilic

Physiology and metabolism

oxygen tolerance

@refoxygen toleranceconfidence
69480anaerobe99.998
69645anaerobe

spore formation

  • @ref: 69480
  • spore formation: no
  • confidence: 99.997

observation

  • @ref: 69645
  • observation: Cells grow as single cells or in pairs

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6964517234glucose+assimilation
6838016199urea-hydrolysis
6838029016arginine-hydrolysis
6838016024D-mannose-fermentation
6838016634raffinose-fermentation
6838029985L-glutamate-degradation
6838017632nitrate-reduction
6838027897tryptophan-energy source

metabolite production

@refChebi-IDmetaboliteproduction
6964530089acetateyes
6964515740formateyes
6838035581indoleno

metabolite tests

  • @ref: 68380
  • Chebi-ID: 35581
  • metabolite: indole
  • indole test: -

enzymes

@refvalueactivityec
68380serine arylamidase-
68380glutamyl-glutamate arylamidase-
68380histidine arylamidase-
68380glycin arylamidase-
68380alanine arylamidase-3.4.11.2
68380tyrosine arylamidase-
68380pyrrolidonyl arylamidase-3.4.19.3
68380leucine arylamidase-3.4.11.1
68380phenylalanine arylamidase-
68380leucyl glycin arylamidase-3.4.11.1
68380proline-arylamidase+3.4.11.5
68380L-arginine arylamidase-
68380tryptophan deaminase-4.1.99.1
68380alpha-fucosidase-3.2.1.51
68380glutamate decarboxylase-4.1.1.15
68380N-acetyl-beta-glucosaminidase-3.2.1.52
68380beta-glucuronidase-3.2.1.31
68380alpha-arabinosidase-3.2.1.55
68380beta-glucosidase-3.2.1.21
68380alpha-glucosidase-3.2.1.20
68380beta-Galactosidase 6-phosphate-
68380beta-galactosidase-3.2.1.23
68380alpha-galactosidase-3.2.1.22
68380arginine dihydrolase-3.5.3.6
68380urease-3.5.1.5

API rID32A

@refUREADH Argalpha GALbeta GALbeta GPalpha GLUbeta GLUalpha ARAbeta GURbeta NAGMNERAFGDCalpha FUCNITINDPALArgAProALGAPheALeuAPyrATyrAAlaAGlyAHisAGGASerA
66464----------------+/--+----------
66464----------------+/--+----------

Isolation, sampling and environmental information

isolation

@refsample typecountryorigin.countrycontinentlatitudelongitudegeographic locationenrichment cultureenrichment culture durationenrichment culture temperatureisolation procedure
66464intestinal tract (ileal contents) of a three month-old healthy pigUSAUSANorth America42.0308-93.6319
67770Ileal content of a pig in AmesUSAUSANorth AmericaIowa
69645Ileal content of a pigUSAUSANorth AmericaAmes, IowaHog gastric mucin medium3-5 days37anaerobic

isolation source categories

Cat1Cat2Cat3
#Host#Mammals#Suidae (Pig,Swine)
#Host Body-Site#Gastrointestinal tract

Sequence information

16S sequences

  • @ref: 66464
  • description: Lachnospiraceae bacterium DSM 104945 16S ribosomal RNA gene, partial sequence
  • accession: MN537470
  • length: 1380
  • database: ena
  • NCBI tax ID: 2652301

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Clostridiaceae bacterium 68-1-52606628.3wgspatric2606628
66792Suipraeoptans intestinalis 68-1-5GCA_009696485contigncbi2606628
69645Whole genome shotgunVULY00000000ncbi2606628

GC content

  • @ref: 67770
  • GC-content: 47.7
  • method: genome sequence analysis

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno55no
motileno89.786no
gram-positiveyes92.207no
anaerobicyes99.697no
aerobicno99.101no
halophileno75.474no
spore-formingno75.524no
glucose-utilyes88.988no
flagellatedno96.556no
thermophileno97.417yes
glucose-fermentyes83.126no

External links

@ref: 66464

culture collection no.: DSM 104945, JCM 34387

straininfo link

  • @ref: 112102
  • straininfo: 399464

Reference

@idauthorstitledoi/urlcatalogue
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
66464Curators of the DSMZhttps://www.dsmz.de/collection/catalogue/details/culture/DSM-104945Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 104945)
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68380Automatically annotated from API rID32A
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
69645David Wylensek, Thomas C. A. Hitch, Thomas Riedel, Afrizal Afrizal, Neeraj Kumar, Esther Wortmann, Tianzhe Liu, Saravanan Devendran, Till R. Lesker, Sara B. Hernández, Viktoria Heine, Eva M. Buhl, Paul M. D'Agostino, Fabio Cumbo, Thomas Fischöder, Marzena Wyschkon, Torey Looft, Valeria R. Parreira, Birte Abt, Heidi L. Doden, Lindsey Ly, João M. P. Alves, Markus Reichlin, Krzysztof Flisikowski, Laura Navarro Suarez, Anthony P. Neumann, Garret Suen, Tomas de Wouters, Sascha Rohn, Ilias Lagkouvardos, Emma Allen-Vercoe, Cathrin Spröer, Boyke Bunk, Anja J. Taverne-Thiele, Marcel Giesbers, Jerry M. Wells, Klaus Neuhaus, Angelika Schnieke, Felipe Cava, Nicola Segata, Lothar Elling, Till Strowig, Jason M. Ridlon, Tobias A. M. Gulder, Jörg Overmann, Thomas ClavelA collection of bacterial isolates from the pig intestine reveals functional and taxonomic diversity10.1038/s41467-020-19929-w
112102Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.StrainInfo: A central database for resolving microbial strain identifiers10.60712/SI-ID399464.1