Strain identifier

BacDive ID: 159623

Type strain: Yes

Species: Pseudomonas saxonica

Strain Designation: WS 5072

Strain history: <- M. Wenning, BFLM, TU Munich, Freising-Weihenstephan, Germany; WS5072 <-

NCBI tax ID(s): 2600598 (species)

For citation purpose refer to the digital object identifier (doi) of the current version.
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General

@ref: 66458

BacDive-ID: 159623

DSM-Number: 108989

keywords: genome sequence, 16S sequence, Bacteria, aerobe, mesophilic, motile

description: Pseudomonas saxonica WS 5072 is an aerobe, mesophilic, motile bacterium that was isolated from skim milk concentrate.

NCBI tax id

  • NCBI tax id: 2600598
  • Matching level: species

strain history

  • @ref: 66458
  • history: <- M. Wenning, BFLM, TU Munich, Freising-Weihenstephan, Germany; WS5072 <-

doi: 10.13145/bacdive159623.20250331.9.3

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Pseudomonadales
  • family: Pseudomonadaceae
  • genus: Pseudomonas
  • species: Pseudomonas saxonica
  • full scientific name: Pseudomonas saxonica Hofmann et al. 2020

@ref: 66458

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Pseudomonadales

family: Pseudomonadaceae

genus: Pseudomonas

species: Pseudomonas saxonica

full scientific name: Pseudomonas saxonica

strain designation: WS 5072

type strain: yes

Morphology

cell morphology

@refmotilityconfidencegram stain
125438yes91.092
12543899negative
12543997.4negative

multimedia

  • @ref: 66458
  • multimedia content: https://www.dsmz.de/microorganisms/photos/DSM_108989.jpg
  • caption: DSM 108989 in DSMZ medium 535 at 28 °C
  • intellectual property rights: © Leibniz-Institut DSMZ

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
66458TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535)yeshttps://mediadive.dsmz.de/medium/535Name: TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Distilled water
66458TRYPTONE SOYA BROTH (TSB) (DSMZ Medium 545)yeshttps://mediadive.dsmz.de/medium/545Name: TRYPTONE SOYA BROTH (TSB) (DSMZ Medium 545) Composition: Casein peptone 17.0 g/l NaCl 5.0 g/l Soy peptone 3.0 g/l D(+)-Glucose 2.5 g/l K2HPO4 2.5 g/l Distilled water

culture temp

  • @ref: 66458
  • growth: positive
  • type: growth
  • temperature: 28

Physiology and metabolism

oxygen tolerance

  • @ref: 125438
  • oxygen tolerance: aerobe
  • confidence: 90.896

spore formation

  • @ref: 125439
  • spore formation: no
  • confidence: 96

Isolation, sampling and environmental information

isolation

  • @ref: 66458
  • sample type: skim milk concentrate
  • geographic location: Leppersdorf (48° 38' 48" N, 9° 25' 44.0" E)
  • country: Germany
  • origin.country: DEU
  • continent: Europe

isolation source categories

  • Cat1: #Engineered
  • Cat2: #Food production
  • Cat3: #Dairy product

Sequence information

16S sequences

  • @ref: 66458
  • description: Pseudomonas saxonica strain DSM 108989 RNA polymerase sigma factor RpoD (rpoD) gene, partial cds
  • accession: MN068213
  • length: 683
  • database: nuccore
  • NCBI tax ID: 2600598

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Pseudomonas saxonica DSM 108989GCA_007858365contigncbi2600598
66792Pseudomonas sp. DSM 1089892600598.3wgspatric2600598

Genome-based predictions

predictions

@refmodeltraitdescriptionpredictionconfidencetraining_data
125438gram-positivegram-positivePositive reaction to Gram-stainingno99no
125438anaerobicanaerobicAbility to grow under anoxygenic conditions (including facultative anaerobes)no98.221no
125438spore-formingspore-formingAbility to form endo- or exosporesno87.23no
125438aerobicaerobicAbility to grow under oxygenic conditions (including facultative aerobes)yes90.896no
125438thermophilethermophilicAbility to grow at temperatures above or equal to 45°Cno98yes
125438motile2+flagellatedAbility to perform flagellated movementyes91.092no
125439BacteriaNetspore_formationAbility to form endo- or exosporesno96
125439BacteriaNetmotilityAbility to perform movementyes80.4
125439BacteriaNetgram_stainReaction to gram-stainingnegative97.4
125439BacteriaNetoxygen_toleranceOxygenic conditions needed for growthobligate aerobe87.9

External links

@ref: 66458

culture collection no.: DSM 108989, LMG 31234

straininfo link

  • @ref: 112097
  • straininfo: 400476

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny31730030Pseudomonas saxonica sp. nov., isolated from raw milk and skimmed milk concentrate.Hofmann K, Huptas C, Doll EV, Scherer S, Wenning MInt J Syst Evol Microbiol10.1099/ijsem.0.0038512020Animals, Bacterial Typing Techniques, Base Composition, Cattle, DNA, Bacterial/genetics, Fatty Acids/chemistry, Female, *Food Microbiology, Genes, Bacterial, Germany, Milk/*microbiology, Multilocus Sequence Typing, Nucleic Acid Hybridization, Phospholipids/chemistry, *Phylogeny, Pseudomonas/*classification/isolation & purification, Quinones/chemistry, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNABiotechnology
Phylogeny34097596Pseudomonas paraversuta sp. nov. isolated from refrigerated dry-aged beef.Lick S, Wibberg D, Winkler A, Blom J, Grimmler C, Goesmann A, Kalinowski J, Krockel LInt J Syst Evol Microbiol10.1099/ijsem.0.0048222021Animals, Bacterial Typing Techniques, Base Composition, Cattle, DNA, Bacterial/genetics, Fatty Acids/chemistry, *Food Microbiology, Genes, Bacterial, Germany, Nucleic Acid Hybridization, Phospholipids/chemistry, *Phylogeny, Pseudomonas/*classification/isolation & purification, RNA, Ribosomal, 16S/genetics, Red Meat/*microbiology, Sequence Analysis, DNA, Ubiquinone/chemistryBiotechnology

Reference

@idauthorstitledoi/urlcatalogue
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
66458Curators of the DSMZhttps://www.dsmz.de/collection/catalogue/details/culture/DSM-108989Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 108989)
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
112097Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.StrainInfo: A central database for resolving microbial strain identifiers10.60712/SI-ID400476.1
125438Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg OvermannPredicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets10.1101/2024.08.12.607695
125439Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardydeepG: Deep Learning for Genome Sequence Data. R package version 0.3.1https://github.com/GenomeNet/deepG