Strain identifier

BacDive ID: 159618

Type strain: Yes

Species: Actinomadura sporangiiformans

Strain Designation: NEAU-Jh2-5

Strain history: C. Liu; Northeast Agric. Univ., China; NEAU-Jh2-5.

NCBI tax ID(s): 1544728 (species)

For citation purpose refer to the digital object identifier (doi) of the current version.
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General

@ref: 66448

BacDive-ID: 159618

DSM-Number: 111300

keywords: 16S sequence, Bacteria, mesophilic

description: Actinomadura sporangiiformans NEAU-Jh2-5 is a mesophilic bacterium that was isolated from muddy soil collected.

NCBI tax id

  • NCBI tax id: 1544728
  • Matching level: species

strain history

@refhistory
66448<-JCM<- C. Liu; Northeast Agric. Univ., China; NEAU-Jh2-5.
67770C. Liu; Northeast Agric. Univ., China; NEAU-Jh2-5.

doi: 10.13145/bacdive159618.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Streptosporangiales
  • family: Thermomonosporaceae
  • genus: Actinomadura
  • species: Actinomadura sporangiiformans
  • full scientific name: Actinomadura sporangiiformans Zhao et al. 2016

@ref: 66448

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Streptosporangiales

family: Thermomonosporaceae

genus: Actinomadura

species: Actinomadura sporangiiformans

full scientific name: Actinomadura sporangiiformans Zhao et al. 2016

strain designation: NEAU-Jh2-5

type strain: yes

Morphology

multimedia

  • @ref: 66448
  • multimedia content: https://www.dsmz.de/microorganisms/photos/DSM_111300.jpg
  • caption: Medium 554 28°C
  • intellectual property rights: © Leibniz-Institut DSMZ

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
66448N-Z-AMINE-MEDIUM (DSMZ Medium 554)yeshttps://mediadive.dsmz.de/medium/554Name: N-Z-AMINE-MEDIUM (DSMZ Medium 554) Composition: Starch 20.0 g/l Agar 20.0 g/l Glucose 10.0 g/l N-Z amine 5.0 g/l Yeast extract 5.0 g/l CaCO3 1.0 g/l Distilled water
66448TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535)yeshttps://mediadive.dsmz.de/medium/535Name: TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Distilled water
66448ROLLED OATS MINERAL MEDIUM (DSMZ Medium 84)yeshttps://mediadive.dsmz.de/medium/84Name: ROLLED OATS MINERAL MEDIUM (DSMZ Medium 84) Composition: Agar 20.0 g/l Rolled oats 20.0 g/l ZnSO4 x 7 H2O 0.001 g/l MnCl2 x 4 H2O 0.001 g/l FeSO4 x 7 H2O 0.001 g/l Distilled water

culture temp

@refgrowthtypetemperaturerange
66448positivegrowth28mesophilic
67770positivegrowth28mesophilic

Physiology and metabolism

observation

  • @ref: 67770
  • observation: quinones: MK-9(H6), MK-9(H8), MK-9(H4)

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentlatitudelongitude
66448muddy soil collectedriverbank in Jiaohe, Jilin Province, north ChinaChinaCHNAsia44.25127.55
67770Muddy soil from a riverbank in JiaoheJilin Province, north ChinaChinaCHNAsia

isolation source categories

Cat1Cat2Cat3
#Environmental#Terrestrial#Mud (Sludge)
#Environmental#Terrestrial#Soil

Safety information

risk assessment

  • @ref: 66448
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 66448
  • description: Actinomadura sporangiiformans strain NEAU-Jh2-5 16S ribosomal RNA gene, partial sequence
  • accession: KM000834
  • length: 1511
  • database: ena
  • NCBI tax ID: 1544728

GC content

@refGC-contentmethod
6644867.6thermal denaturation, midpoint method (Tm)
6777067-68.2thermal denaturation, midpoint method (Tm)

External links

@ref: 66448

culture collection no.: DSM 111300, CGMCC 4.7211, JCM 30342

straininfo link

  • @ref: 112092
  • straininfo: 402502

literature

  • topic: Phylogeny
  • Pubmed-ID: 26373415
  • title: Actinomadura jiaoheensis sp. nov. and Actinomadura sporangiiformans sp. nov., two novel actinomycetes isolated from muddy soil and emended description of the genus Actinomadura.
  • authors: Zhao J, Guo L, Sun P, Han C, Bai L, Liu C, Li Y, Xiang W, Wang X
  • journal: Antonie Van Leeuwenhoek
  • DOI: 10.1007/s10482-015-0585-1
  • year: 2015
  • mesh: Actinobacteria/*classification/genetics/*isolation & purification/physiology, Bacterial Typing Techniques, China, Cluster Analysis, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, Microscopy, Electron, Scanning, Molecular Sequence Data, Nucleic Acid Hybridization, Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, *Soil Microbiology
  • topic2: Genetics

Reference

@idauthorstitledoi/urlcatalogue
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
66448Curators of the DSMZhttps://www.dsmz.de/collection/catalogue/details/culture/DSM-111300Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 111300)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
112092Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.StrainInfo: A central database for resolving microbial strain identifiers10.60712/SI-ID402502.1