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Strain identifier

BacDive ID: 159549

Type strain: Yes

Species: Mycoplasma procyoni

Strain Designation: LR5794

Strain history: <- D. V. Volokhov; Dept. LMD, Center for Biologics Evaluation and Research; LR5794 <- H. L. Ruhnke; Ontario Ministry of Health, Rexdale, Ontario, Canada; LR5794

NCBI tax ID(s): 568784 (species)

For citation purpose refer to the digital object identifier (doi) of the current version.
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General

@ref: 66375

BacDive-ID: 159549

DSM-Number: 106703

keywords: genome sequence, 16S sequence, Bacteria, facultative anaerobe, mesophilic, colony-forming

description: Mycoplasma procyoni LR5794 is a facultative anaerobe, mesophilic bacterium that forms fried-egg-shaped colonies and was isolated from oral cavity of raccoon .

NCBI tax id

  • NCBI tax id: 568784
  • Matching level: species

strain history: <- D. V. Volokhov; Dept. LMD, Center for Biologics Evaluation and Research; LR5794 <- H. L. Ruhnke; Ontario Ministry of Health, Rexdale, Ontario, Canada; LR5794

doi: 10.13145/bacdive159549.20221219.7.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/mycoplasmatota
  • domain: Bacteria
  • phylum: Mycoplasmatota
  • class: Mollicutes
  • order: Mycoplasmatales
  • family: Mycoplasmataceae
  • genus: Mycoplasma
  • species: Mycoplasma procyoni
  • full scientific name: Mycoplasma procyoni Volokhov et al. 2020

@ref: 66375

domain: Bacteria

phylum: Tenericutes

class: Mollicutes

order: Mycoplasmatales

family: Mycoplasmataceae

genus: Mycoplasma

species: Mycoplasma procyoni

full scientific name: Mycoplasma procyoni Volokhov et al. 2020

strain designation: LR5794

type strain: yes

Morphology

cell morphology

  • @ref: 69480
  • gram stain: positive
  • confidence: 35.507

colony morphology

  • @ref: 68267
  • colony shape: fried-egg-shaped
  • incubation period: 5-7 days
  • medium used: PPLO agar

Culture and growth conditions

culture medium

  • @ref: 66375
  • name: SP4-Z MEDIUM (DSMZ Medium 1076b)
  • growth: yes
  • link: https://mediadive.dsmz.de/medium/1076b
  • composition: Name: SP4-Z MEDIUM (DSMZ Medium 1076b) Composition: Agar 10.0 g/l Tryptone 10.0 g/l Bacto peptone 5.0 g/l PPLO broth 3.5 g/l Urea 2.0 g/l Glucose 1.0 g/l None 1.0 g/l Yeast extract 0.4 g/l DNA 0.2 g/l CMRL 1066 Fetal bovine serum Swine serum Distilled water

culture temp

@refgrowthtypetemperaturerange
66375positivegrowth35mesophilic
68267positivegrowth31-40
68267positiveoptimum35-37mesophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 68267
  • oxygen tolerance: facultative anaerobe

spore formation

  • @ref: 69480
  • spore formation: no
  • confidence: 98.437

halophily

  • @ref: 69480
  • halophily level: non-halophilic
  • confidence: 96.564

observation

@refobservation
68267Bacterial cells lack a cell wall
68267Serum or sterol is required for sustained growth

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6826717234glucose+builds acid from
6826729016arginine-hydrolysis
6826716199urea-hydrolysis

antibiotic resistance

@refChEBImetaboliteis antibioticis resistantresistance conc.is sensitive
6826718208penicillin gyesyes1.5-2.0 g/L
6826727729digitoninyesyes

Isolation, sampling and environmental information

isolation

@refsample typehost speciesgeographic locationcountryorigin.countrycontinentlatitudelongitudeenrichment cultureenrichment culture compositionenrichment culture durationenrichment culture temperature
66375oral cavity of raccoon (Procyon lotor)Procyon lotorOntario, GuelphCanadaCANNorth America43.549-80.25
68267oral cavity of raccoon (Procyon lotor)USAUSANorth AmericaPPLO brothcontaining glucose and penicillin (1.5-2 mg/mL)1-2 weeks36

isolation source categories

Cat1Cat2
#Host Body-Site#Oral cavity and airways
#Host#Mammals

Sequence information

16S sequences

  • @ref: 66375
  • description: Mycoplasma procyoni strain LR5794 16S ribosomal RNA gene and 16S-23S ribosomal RNA intergenic spacer, partial sequence
  • accession: FJ226566
  • length: 1634
  • database: ena
  • NCBI tax ID: 568784

Genome sequences

  • @ref: 66792
  • description: Mycoplasma procyoni LR5794
  • accession: GCA_017052595
  • assembly level: contig
  • database: ncbi
  • NCBI tax ID: 568784

GC content

  • @ref: 68267
  • GC-content: 35.33
  • method: high performance liquid chromatography (HPLC)

External links

@ref: 66375

culture collection no.: DSM 106703, NCTC 14309, ATCC TSD-141

literature

  • topic: Phylogeny
  • Pubmed-ID: 31828363
  • title: Acholeplasma equirhinis sp. nov. isolated from respiratory tract of horse (Equus caballus) and Mycoplasma procyoni sp. nov. isolated from oral cavity of raccoon (Procyon lotor).
  • authors: Volokhov DV, Gao Y, Davidson MK, Chizhikov VE
  • journal: Arch Microbiol
  • DOI: 10.1007/s00203-019-01786-x
  • year: 2019
  • mesh: Acholeplasma/classification/genetics/*isolation & purification, Animals, Canada, DNA, Bacterial/genetics, Horses/*microbiology, Mouth/*microbiology, Mycoplasma/classification/genetics/*isolation & purification, Nasopharynx/*microbiology, Phylogeny, RNA, Ribosomal, 16S/genetics, Raccoons/*microbiology, Sequence Analysis, DNA, United Kingdom
  • topic2: Enzymology

Reference

@idauthorstitledoi/urlcataloguejournal
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
66375Curators of the DSMZhttps://www.dsmz.de/collection/catalogue/details/culture/DSM-106703Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 106703)
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
68267Dmitriy V. Volokhov, Yamei Gao, Maureen K. Davidson & Vladimir E. ChizhikovAcholeplasma equirhinis sp. nov. isolated from respiratory tract of horse (Equus caballus) and Mycoplasma procyoni sp. nov. isolated from oral cavity of raccoon (Procyon lotor)10.1007/s00203-019-01786-xArch Microbiol 202: 411-420 2020
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes