Strain identifier
BacDive ID: 159549
Type strain:
Species: Mycoplasma procyoni
Strain Designation: LR5794
Strain history: <- D. V. Volokhov; Dept. LMD, Center for Biologics Evaluation and Research; LR5794 <- H. L. Ruhnke; Ontario Ministry of Health, Rexdale, Ontario, Canada; LR5794
NCBI tax ID(s): 568784 (species)
General
@ref: 66375
BacDive-ID: 159549
DSM-Number: 106703
keywords: genome sequence, 16S sequence, Bacteria, facultative anaerobe, mesophilic, colony-forming
description: Mycoplasma procyoni LR5794 is a facultative anaerobe, mesophilic bacterium that forms fried-egg-shaped colonies and was isolated from oral cavity of raccoon .
NCBI tax id
- NCBI tax id: 568784
- Matching level: species
strain history
- @ref: 66375
- history: <- D. V. Volokhov; Dept. LMD, Center for Biologics Evaluation and Research; LR5794 <- H. L. Ruhnke; Ontario Ministry of Health, Rexdale, Ontario, Canada; LR5794
doi: 10.13145/bacdive159549.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/mycoplasmatota
- domain: Bacteria
- phylum: Mycoplasmatota
- class: Mollicutes
- order: Mycoplasmatales
- family: Mycoplasmataceae
- genus: Mycoplasma
- species: Mycoplasma procyoni
- full scientific name: Mycoplasma procyoni Volokhov et al. 2020
@ref: 66375
domain: Bacteria
phylum: Tenericutes
class: Mollicutes
order: Mycoplasmatales
family: Mycoplasmataceae
genus: Mycoplasma
species: Mycoplasma procyoni
full scientific name: Mycoplasma procyoni Volokhov et al. 2020
strain designation: LR5794
type strain: yes
Morphology
colony morphology
- @ref: 68267
- colony shape: fried-egg-shaped
- incubation period: 5-7 days
- medium used: PPLO agar
Culture and growth conditions
culture medium
- @ref: 66375
- name: SP4-Z MEDIUM (DSMZ Medium 1076b)
- growth: yes
- link: https://mediadive.dsmz.de/medium/1076b
- composition: Name: SP4-Z MEDIUM (DSMZ Medium 1076b) Composition: Agar 10.0 g/l Tryptone 10.0 g/l Bacto peptone 5.0 g/l PPLO broth 3.5 g/l Urea 2.0 g/l Glucose 1.0 g/l None 1.0 g/l Yeast extract 0.4 g/l DNA 0.2 g/l CMRL 1066 Fetal bovine serum Swine serum Distilled water
culture temp
@ref | growth | type | temperature | range |
---|---|---|---|---|
66375 | positive | growth | 35 | mesophilic |
68267 | positive | growth | 31-40 | |
68267 | positive | optimum | 35-37 | mesophilic |
Physiology and metabolism
oxygen tolerance
- @ref: 68267
- oxygen tolerance: facultative anaerobe
observation
@ref | observation |
---|---|
68267 | Bacterial cells lack a cell wall |
68267 | Serum or sterol is required for sustained growth |
metabolite utilization
@ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
---|---|---|---|---|
68267 | 17234 | glucose | + | builds acid from |
68267 | 29016 | arginine | - | hydrolysis |
68267 | 16199 | urea | - | hydrolysis |
antibiotic resistance
@ref | ChEBI | metabolite | is antibiotic | is resistant | resistance conc. | is sensitive |
---|---|---|---|---|---|---|
68267 | 18208 | penicillin g | yes | yes | 1.5-2.0 g/L | |
68267 | 27729 | digitonin | yes | yes |
Isolation, sampling and environmental information
isolation
@ref | sample type | host species | geographic location | country | origin.country | continent | latitude | longitude | enrichment culture | enrichment culture composition | enrichment culture duration | enrichment culture temperature |
---|---|---|---|---|---|---|---|---|---|---|---|---|
66375 | oral cavity of raccoon (Procyon lotor) | Procyon lotor | Ontario, Guelph | Canada | CAN | North America | 43.549 | -80.25 | ||||
68267 | oral cavity of raccoon (Procyon lotor) | USA | USA | North America | PPLO broth | containing glucose and penicillin (1.5-2 mg/mL) | 1-2 weeks | 36 |
isolation source categories
Cat1 | Cat2 |
---|---|
#Host Body-Site | #Oral cavity and airways |
#Host | #Mammals |
Sequence information
16S sequences
- @ref: 66375
- description: Mycoplasma procyoni strain LR5794 16S ribosomal RNA gene and 16S-23S ribosomal RNA intergenic spacer, partial sequence
- accession: FJ226566
- length: 1634
- database: ena
- NCBI tax ID: 568784
Genome sequences
- @ref: 66792
- description: Mycoplasma procyoni LR5794
- accession: GCA_017052595
- assembly level: contig
- database: ncbi
- NCBI tax ID: 568784
GC content
- @ref: 68267
- GC-content: 35.33
- method: high performance liquid chromatography (HPLC)
Genome-based predictions
predictions
trait | prediction | confidence | training_data |
---|---|---|---|
flagellated | no | 97.401 | no |
gram-positive | yes | 68.191 | no |
anaerobic | no | 96.288 | yes |
aerobic | no | 94.521 | yes |
halophile | yes | 51.677 | no |
spore-forming | no | 98.206 | no |
thermophile | no | 96.438 | yes |
glucose-util | yes | 86.846 | no |
motile | no | 92.18 | no |
glucose-ferment | yes | 83.846 | no |
External links
@ref: 66375
culture collection no.: DSM 106703, NCTC 14309, ATCC TSD-141
straininfo link
- @ref: 112028
- straininfo: 397092
literature
- topic: Phylogeny
- Pubmed-ID: 31828363
- title: Acholeplasma equirhinis sp. nov. isolated from respiratory tract of horse (Equus caballus) and Mycoplasma procyoni sp. nov. isolated from oral cavity of raccoon (Procyon lotor).
- authors: Volokhov DV, Gao Y, Davidson MK, Chizhikov VE
- journal: Arch Microbiol
- DOI: 10.1007/s00203-019-01786-x
- year: 2019
- mesh: Acholeplasma/classification/genetics/*isolation & purification, Animals, Canada, DNA, Bacterial/genetics, Horses/*microbiology, Mouth/*microbiology, Mycoplasma/classification/genetics/*isolation & purification, Nasopharynx/*microbiology, Phylogeny, RNA, Ribosomal, 16S/genetics, Raccoons/*microbiology, Sequence Analysis, DNA, United Kingdom
- topic2: Enzymology
Reference
@id | authors | title | doi/url | catalogue | journal |
---|---|---|---|---|---|
20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | 10.1099/ijsem.0.004332 | ||
66375 | Curators of the DSMZ | https://www.dsmz.de/collection/catalogue/details/culture/DSM-106703 | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 106703) | ||
66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | https://diaspora-project.de/progress.html#genomes | ||
68267 | Dmitriy V. Volokhov, Yamei Gao, Maureen K. Davidson & Vladimir E. Chizhikov | Acholeplasma equirhinis sp. nov. isolated from respiratory tract of horse (Equus caballus) and Mycoplasma procyoni sp. nov. isolated from oral cavity of raccoon (Procyon lotor) | 10.1007/s00203-019-01786-x | Arch Microbiol 202: 411-420 2020 | |
112028 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | StrainInfo: A central database for resolving microbial strain identifiers | 10.60712/SI-ID397092.1 |