Strain identifier

BacDive ID: 159514

Type strain: Yes

Species: Pseudomonas carnis

Strain Designation: B4-1

Strain history: <- L. Kroeckel, Max Rubner Institut, Federal Research Institute of Nutrition and Food, Department of Microbiology and Biotechnology; B4-1 <- A. Bantleon and L. Kroeckel

NCBI tax ID(s): 2487355 (species)

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General

@ref: 66339

BacDive-ID: 159514

DSM-Number: 107652

keywords: genome sequence, Bacteria, mesophilic

description: Pseudomonas carnis B4-1 is a mesophilic bacterium that was isolated from poultry meat cuts .

NCBI tax id

  • NCBI tax id: 2487355
  • Matching level: species

strain history

  • @ref: 66339
  • history: <- L. Kroeckel, Max Rubner Institut, Federal Research Institute of Nutrition and Food, Department of Microbiology and Biotechnology; B4-1 <- A. Bantleon and L. Kroeckel

doi: 10.13145/bacdive159514.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Pseudomonadales
  • family: Pseudomonadaceae
  • genus: Pseudomonas
  • species: Pseudomonas carnis
  • full scientific name: Pseudomonas carnis Lick et al. 2020

@ref: 66339

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Pseudomonadales

family: Pseudomonadaceae

genus: Pseudomonas

species: Pseudomonas carnis

full scientific name: Pseudomonas carnis Lick and Kröckel et al. 2020

strain designation: B4-1

type strain: yes

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
66339NUTRIENT AGAR (DSMZ Medium 1)yeshttps://mediadive.dsmz.de/medium/1Name: NUTRIENT AGAR (DSMZ Medium 1) Composition: Agar 15.0 g/l Peptone 5.0 g/l Meat extract 3.0 g/l Distilled water
66339TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535)yeshttps://mediadive.dsmz.de/medium/535Name: TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Distilled water

culture temp

  • @ref: 66339
  • growth: positive
  • type: growth
  • temperature: 25
  • range: mesophilic

Physiology and metabolism

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6836925115malate+assimilation
6836917128adipate-assimilation
6836927689decanoate+assimilation
6836924265gluconate+assimilation
6836917306maltose-assimilation
6836959640N-acetylglucosamine+assimilation
6836916899D-mannitol+assimilation
6836916024D-mannose+assimilation
6836930849L-arabinose+assimilation
6836917634D-glucose+assimilation
683695291gelatin+hydrolysis
683694853esculin+hydrolysis
6836916199urea-hydrolysis
6836929016arginine+hydrolysis
6836917634D-glucose-fermentation
6836927897tryptophan-energy source
6836917632nitrate-reduction

metabolite production

  • @ref: 68369
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

metabolite tests

  • @ref: 68369
  • Chebi-ID: 35581
  • metabolite: indole
  • indole test: -

enzymes

@refvalueactivityec
68369cytochrome oxidase+1.9.3.1
68369gelatinase+
68369beta-glucosidase+3.2.1.21
68369urease-3.5.1.5
68369arginine dihydrolase+3.5.3.6

API 20NE

@refNO3TRPGLU_ FermADH ArgUREESCGELPNPGGLU_ AssimARAMNEMANNAGMALGNTCAPADIMLTCITPACOX
66339---+-++-+++++-++-++-+

Isolation, sampling and environmental information

isolation

  • @ref: 66339
  • sample type: poultry meat cuts (raw material for processed meat products)
  • geographic location: Versmold, NRW
  • country: Germany
  • origin.country: DEU
  • continent: Europe
  • latitude: 52.1678
  • longitude: 8.2739

isolation source categories

Cat1Cat2Cat3
#Engineered#Food production#Meat
#Host#Birds

Sequence information

Genome sequences

  • @ref: 66792
  • description: Pseudomonas carnis B4-1
  • accession: 2897202802
  • assembly level: draft
  • database: img
  • NCBI tax ID: 2487355

GC content

  • @ref: 66339
  • GC-content: 59.8
  • method: sequence analysis

External links

@ref: 66339

culture collection no.: DSM 107652, LMG 30892

straininfo link

  • @ref: 111997
  • straininfo: 407536

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny31922466Pseudomonas carnis sp. nov., isolated from meat.Lick S, Krockel L, Wibberg D, Winkler A, Blom J, Bantleon A, Goesmann A, Kalinowski JInt J Syst Evol Microbiol10.1099/ijsem.0.0039282020Animals, Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Fatty Acids/chemistry, *Food Microbiology, Genes, Bacterial, Germany, Meat/*microbiology, Nucleic Acid Hybridization, Phospholipids/chemistry, *Phylogeny, Poultry, Pseudomonas/*classification/isolation & purification, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Swine, Ubiquinone/chemistryBiotechnology
Phylogeny33480838Pseudomonas paracarnis sp. nov., isolated from refrigerated beef.Lick S, Wibberg D, Winkler A, Blom J, Grimmler C, Goesmann A, Kalinowski J, Krockel LInt J Syst Evol Microbiol10.1099/ijsem.0.0046522021Animals, Bacterial Typing Techniques, Base Composition, Cattle, DNA, Bacterial/genetics, Fatty Acids/chemistry, *Food Microbiology, Nucleic Acid Hybridization, Phospholipids/chemistry, *Phylogeny, Pseudomonas/*classification/isolation & purification, RNA, Ribosomal, 16S/genetics, Red Meat/*microbiology, Sequence Analysis, DNA, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization, Vitamin K 2/analogs & derivatives/chemistryBiotechnology

Reference

@idauthorstitledoi/urlcatalogue
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
66339Curators of the DSMZhttps://www.dsmz.de/collection/catalogue/details/culture/DSM-107652Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 107652)
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
68369Automatically annotated from API 20NE
111997Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.StrainInfo: A central database for resolving microbial strain identifiers10.60712/SI-ID407536.1