Strain identifier

BacDive ID: 159479

Type strain: Yes

Species: Streptomyces triticagri

Strain Designation: NEAU-YY421

Strain history: <- J. Zhao, School of Life Science,Northeast Agricultural University, Harbin, P.R. China; NEAU-YY421 <-

NCBI tax ID(s): 2293568 (species)

For citation purpose refer to the digital object identifier (doi) of the current version.
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General

@ref: 66302

BacDive-ID: 159479

DSM-Number: 106775

keywords: genome sequence, 16S sequence, Bacteria, aerobe, spore-forming, mesophilic

description: Streptomyces triticagri NEAU-YY421 is an aerobe, spore-forming, mesophilic bacterium that builds an aerial mycelium and was isolated from soil.

NCBI tax id

  • NCBI tax id: 2293568
  • Matching level: species

strain history

  • @ref: 66302
  • history: <- J. Zhao, School of Life Science,Northeast Agricultural University, Harbin, P.R. China; NEAU-YY421 <-

doi: 10.13145/bacdive159479.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Kitasatosporales
  • family: Streptomycetaceae
  • genus: Streptomyces
  • species: Streptomyces triticagri
  • full scientific name: Streptomyces triticagri Han et al. 2020

@ref: 66302

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Streptomycetales

family: Streptomycetaceae

genus: Streptomyces

species: Streptomyces triticagri

full scientific name: Streptomyces triticagri Han et al. 2019

strain designation: NEAU-YY421

type strain: yes

Morphology

cell morphology

@refmotilityconfidencegram staincell lengthcell widthcell shape
69480no90.493
69480100positive
69500nopositive0.7-1.0 µm0.5-0.6 µmspore-shaped

colony morphology

@refcolony colormedium used
69326Black brown (8022)ISP 7
69326Black brown (802)ISP 4
69326Grey brown (8019)ISP 3
69326Grey brown (8019), sepia brown (8014)ISP 5
69326Ivory (1014), black brown (8022)ISP 6
69326Nut brown (8011)suter without tyrosine
69326Olive brown (8008)suter with tyrosine
69326Sepia brown (8014), black brown (8022)ISP 2

multicellular morphology

@refforms multicellular complexcomplex namecomplex colormedium namefurther description
69326yesAerial myceliumSignal white (9003), grey white (9002)ISP 2
69326yesAerial myceliumSignal white (9003)ISP 3
69326yesAerial myceliumCream (9001), signal white (9003)ISP 4
69326yesAerial myceliumCream (9001), silk grey (7044)ISP 5
69326yesAerial myceliumStone grey (7030)ISP 6
69326yesAerial myceliumSignal grey (7004), signal white (9003)ISP 7
69326yesAerial myceliumStone grey (7030)suter with tyrosine
69326yesAerial myceliumStone grey (7030)suter without tyrosine
69500yesbranched substrate hyphaeISP 3
69500yesaerial myceliumISP 3differentiated into straight or flexuous spore chains consisting of cylindrical spores

pigmentation

@refproductionnamecolor
69326noMelanin
69326yessoluble pigmentSignal brown (8002), fawn brown (8007), green brown (8000), khaki grey (7008), ochre yellow (1024)

multimedia

  • @ref: 66302
  • multimedia content: https://www.dsmz.de/microorganisms/photos/DSM_106775.jpg
  • caption: Medium 65 28°C
  • intellectual property rights: © Leibniz-Institut DSMZ

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
66302GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)yeshttps://mediadive.dsmz.de/medium/65Name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) Composition: Agar 18.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water
69500ISP 1yes
69500ISP 2yes
69500ISP 3yes
69500ISP 4yes
69500ISP 5yes
69500ISP 6yes

culture temp

@refgrowthtypetemperaturerange
66302positivegrowth28mesophilic
69500positivegrowth10.0-40.0
69500positiveoptimum28mesophilic

culture pH

@refabilitytypepH
69500positivegrowth5.0-9.0
69500positiveoptimum7

Physiology and metabolism

oxygen tolerance

  • @ref: 69500
  • oxygen tolerance: aerobe

spore formation

@refspore formationconfidencespore descriptiontype of spore
69480yes100
69481yes100
69500yesstraight spore chains of cylindrical spores (0.5-0.6×0.7-1.0 µm)spore

halophily

@refsaltgrowthtested relationconcentration
69326NaClpositivegrowth0-5 %
69500NaClpositivegrowth0.0-13.0 %(w/v)
69500NaClpositiveoptimum0.0-1.0 %(w/v)

observation

  • @ref: 69500
  • observation: The polar lipid profile contains aminophopholipid, diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol and phosphatidylinositol mannoside.

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6932622599arabinose+growth
6932662968cellulose+/-growth
6932628757fructose+growth
6932617234glucose+growth
6932617268inositol+growth
6932637684mannose+/-growth
6932616634raffinose+/-growth
6932626546rhamnose+growth
6932617992sucrose+/-growth
6932618222xylose+growth
695004853esculin-hydrolysis
6950053424tween 20-hydrolysis
6950015428glycine-nitrogen source
6950017203L-proline-nitrogen source
6950016857L-threonine-nitrogen source
6950017895L-tyrosine-nitrogen source
6950017632nitrate-reduction
6950015824D-fructose+carbon source
6950012936D-galactose+carbon source
6950017634D-glucose+carbon source
6950016899D-mannitol+carbon source
6950016024D-mannose+carbon source
6950016988D-ribose+carbon source
6950017924D-sorbitol+carbon source
6950065327D-xylose+carbon source
6950016813galactitol+carbon source
6950030849L-arabinose+carbon source
6950062345L-rhamnose+carbon source
6950017716lactose+carbon source
6950017306maltose+carbon source
6950017268myo-inositol+carbon source
6950016634raffinose+carbon source
6950017992sucrose+carbon source
6950028017starch+hydrolysis
6950053423tween 40+hydrolysis
6950053426tween 80+hydrolysis
6950016919creatine+nitrogen source
6950016977L-alanine+nitrogen source
6950016467L-arginine+nitrogen source
6950017196L-asparagine+nitrogen source
6950029991L-aspartate+nitrogen source
6950029985L-glutamate+nitrogen source
6950018050L-glutamine+nitrogen source
6950017115L-serine+nitrogen source
6837917632nitrate+reduction
683794853esculin-hydrolysis
6837916199urea-hydrolysis
683795291gelatin+hydrolysis
6837917634D-glucose-fermentation
6837916988D-ribose-fermentation
6837965327D-xylose-fermentation
6837916899D-mannitol-fermentation
6837917306maltose-fermentation
6837917716lactose-fermentation
6837917992sucrose-fermentation
6837928087glycogen-fermentation

metabolite production

  • @ref: 69500
  • Chebi-ID: 16136
  • metabolite: hydrogen sulfide
  • production: no

enzymes

@refvalueactivityec
68379gelatinase+
68379urease-3.5.1.5
68379beta-glucosidase-3.2.1.21
68379N-acetyl-beta-glucosaminidase+3.2.1.52
68379alpha-glucosidase+3.2.1.20
68379beta-galactosidase+3.2.1.23
68379beta-glucuronidase-3.2.1.31
68379alkaline phosphatase+3.1.3.1
68379pyrrolidonyl arylamidase-3.4.19.3
68379pyrazinamidase-3.5.1.B15
69500urease+3.5.1.5
69500catalase+1.11.1.6
68382alkaline phosphatase+3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)+
68382leucine arylamidase+3.4.11.1
68382valine arylamidase+
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase+3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase+3.2.1.20
68382beta-glucosidase+3.2.1.21
68382N-acetyl-beta-glucosaminidase+3.2.1.52
68382alpha-mannosidase+3.2.1.24
68382alpha-fucosidase-3.2.1.51

API coryne

@refNITPYZPYRAPALbeta GURbeta GALalpha GLUbeta NAGESCUREGELGLURIBXYLMANMALLACSACGLYG
69326+--+-+++--+--------

API zym

@refAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
69326++++/-+++/-+/-+/-++-+-++++-

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentlatitudelongitudeenrichment cultureenrichment culture compositionenrichment culture durationenrichment culture temperature
66302soilcentral China, Henan province, Zhumadian (33° 5' 50.9'' N, 113° 57' 3'' E)ChinaCHNAsia33.0975113.951
69500soil sample rhizosphere of Triticum aestivumZhumadian, Henan ProvinceChinaCHNAsiaDPADPA supplemented with cycloheximide (50 mg/l) and nalidixic acid (20 mg/l).28 days28

isolation source categories

  • Cat1: #Environmental
  • Cat2: #Terrestrial
  • Cat3: #Soil

Sequence information

16S sequences

  • @ref: 66302
  • description: Streptomyces sp. NEAU-YY421 16S ribosomal RNA gene, partial sequence
  • accession: MH144587
  • length: 1520
  • database: ena
  • NCBI tax ID: 2293568

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Streptomyces triticagri NEAU-YY421GCA_003429565scaffoldncbi2293568
66792Streptomyces triticagri NEAU-YY4212862290372draftimg2293568
69500genome sequenceQUAK00000000ncbi2293568

GC content

  • @ref: 69500
  • GC-content: 71.1
  • method: genome sequence analysis

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingyes100no
motileno92.922no
flagellatedno97.694no
gram-positiveyes91.235no
anaerobicno99.367no
aerobicyes93.702no
halophileno90.717no
spore-formingyes94.722no
glucose-utilyes86.39no
thermophileno98.313yes
glucose-fermentno87.745yes

External links

@ref: 66302

culture collection no.: DSM 106775, CGMCC 4.7476

straininfo link

  • @ref: 111969
  • straininfo: 402380

literature

  • topic: Phylogeny
  • Pubmed-ID: 31613740
  • title: Streptomyces triticagri sp. nov. and Streptomyces triticirhizae sp. nov., two novel Actinobacteria isolated from the rhizosphere soil of wheat (Triticum aestivum L.).
  • authors: Han C, Yu Z, Zhao J, Shi H, Hu J, Yu B, Song J, Shen Y, Xiang W, Wang X
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijsem.0.003727
  • year: 2020
  • mesh: Bacterial Typing Techniques, Base Composition, Cell Wall/chemistry, China, DNA, Bacterial/genetics, Diaminopimelic Acid/chemistry, Fatty Acids/chemistry, Genes, Bacterial, Nucleic Acid Hybridization, *Phylogeny, RNA, Ribosomal, 16S/genetics, *Rhizosphere, Sequence Analysis, DNA, *Soil Microbiology, Streptomyces/*classification/isolation & purification, Triticum/*microbiology
  • topic2: Transcriptome

Reference

@idauthorstitledoi/urlcatalogue
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
66302Curators of the DSMZhttps://www.dsmz.de/collection/catalogue/details/culture/DSM-106775Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 106775)
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
68379Automatically annotated from API Coryne
68382Automatically annotated from API zym
69326Wink, J.Compendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweighttps://cdn.dsmz.de/wink/DSM%20106775.pdf
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. MünchPredictions based on the model GenomeNet Sporulation v. 110.21203/rs.3.rs-2527258/v1
69500Kallayanee Naloka, Pattaraporn Yukphan, Minenosuke Matsutani, Kazunobu Matsushita, Gunjana TheeragoolStreptomyces triticagri sp. nov. and Streptomyces triticirhizae sp. nov., two novel Actinobacteria isolated from the rhizosphere soil of wheat (Triticum aestivum L.)10.1099/ijsem.0.003727
111969Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.StrainInfo: A central database for resolving microbial strain identifiers10.60712/SI-ID402380.1