Strain identifier

BacDive ID: 159212

Type strain: Yes

Species: Acholeplasma equirhinis

Strain Designation: N93

Strain history: <- D. V. Volokhov, Dept. LMD, Center for Biologics Evaluation and Research; N93 <- R. M. Lemcke and D. V. Volokhov

NCBI tax ID(s): 555393 (species)

For citation purpose refer to the digital object identifier (doi) of the current version.
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General

@ref: 66035

BacDive-ID: 159212

DSM-Number: 106692

keywords: genome sequence, 16S sequence, Bacteria, facultative anaerobe, mesophilic, colony-forming

description: Acholeplasma equirhinis N93 is a facultative anaerobe, mesophilic bacterium that forms fried-egg-shaped colonies and was isolated from nasopharyngeal swab of a horse.

NCBI tax id

  • NCBI tax id: 555393
  • Matching level: species

strain history

  • @ref: 66035
  • history: <- D. V. Volokhov, Dept. LMD, Center for Biologics Evaluation and Research; N93 <- R. M. Lemcke and D. V. Volokhov

doi: 10.13145/bacdive159212.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/mycoplasmatota
  • domain: Bacteria
  • phylum: Mycoplasmatota
  • class: Mollicutes
  • order: Acholeplasmatales
  • family: Acholeplasmataceae
  • genus: Acholeplasma
  • species: Acholeplasma equirhinis
  • full scientific name: Acholeplasma equirhinis Volokhov et al. 2020

@ref: 66035

domain: Bacteria

phylum: Tenericutes

class: Mollicutes

order: Acholeplasmatales

family: Acholeplasmataceae

genus: Acholeplasma

species: Acholeplasma equirhinis

full scientific name: Acholeplasma equirhinis Volokhov et al. 2020

strain designation: N93

type strain: yes

Morphology

colony morphology

  • @ref: 68267
  • colony size: 1 mm
  • colony shape: fried-egg-shaped
  • incubation period: 5-7 days
  • medium used: PPLO agar

Culture and growth conditions

culture medium

  • @ref: 66035
  • name: SP4-Z MEDIUM (DSMZ Medium 1076b)
  • growth: yes
  • link: https://mediadive.dsmz.de/medium/1076b
  • composition: Name: SP4-Z MEDIUM (DSMZ Medium 1076b) Composition: Agar 10.0 g/l Tryptone 10.0 g/l Bacto peptone 5.0 g/l PPLO broth 3.5 g/l Urea 2.0 g/l Glucose 1.0 g/l None 1.0 g/l Yeast extract 0.4 g/l DNA 0.2 g/l CMRL 1066 Fetal bovine serum Swine serum Distilled water

culture temp

@refgrowthtypetemperaturerange
66035positivegrowth35mesophilic
68267positivegrowth22-41
68267positiveoptimum35-37mesophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 68267
  • oxygen tolerance: facultative anaerobe

observation

@refobservation
68267Bacterial cells lack a cell wall
68267Serum or sterol is required for sustained growth

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6826717234glucose+builds acid from
6826729016arginine-hydrolysis
6826716199urea-hydrolysis

antibiotic resistance

  • @ref: 68267
  • ChEBI: 18208
  • metabolite: penicillin g
  • is antibiotic: yes
  • is resistant: yes
  • resistance conc.: 1.5-2.0 g/L

Isolation, sampling and environmental information

isolation

@refsample typecountryorigin.countrycontinentlatitudelongitudeenrichment cultureenrichment culture compositionenrichment culture durationenrichment culture temperature
66035nasopharyngeal swab of a horseUnited KingdomGBREurope55.3618-3.4433
68267nasopharynx of a thoroughbred horse (Equus caballus)United KingdomGBREuropePPLO brothcontaining glucose and penicillin (1.5-2 mg/mL)1-2 weeks36

isolation source categories

Cat1Cat2Cat3
#Host#Mammals#Equidae (Horse)
#Host Body-Site#Organ#Nose
#Infection#Patient#Swab

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
66035Acholeplasma equirhinis strain N93 16S ribosomal RNA gene and 16S-23S ribosomal RNA intergenic spacer, partial sequenceEU9251591546ena555393
66035Acholeplasma equirhinis strain N93 23S ribosomal RNA gene, partial sequence; 23S-5S ribosomal RNA intergenic spacer, complete sequence; and 5S ribosomal RNA gene, partial sequenceGU9050352890ena555393
66035Acholeplasma equirhinis strain N93 RNA polymerase beta subunit gene, partial cdsFJ2265622098ena555393

Genome sequences

  • @ref: 66792
  • description: Acholeplasma equirhinis N93
  • accession: GCA_017052655
  • assembly level: contig
  • database: ncbi
  • NCBI tax ID: 555393

GC content

  • @ref: 68267
  • GC-content: 36.83
  • method: high performance liquid chromatography (HPLC)

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
flagellatedno98.475no
gram-positiveyes52.636no
anaerobicno78.195no
aerobicno93.523no
halophileyes61.73no
spore-formingno95.785no
thermophileno97.683yes
glucose-utilyes90.603no
motileno92.876no
glucose-fermentyes70.807no

External links

@ref: 66035

culture collection no.: DSM 106692, NCTC 14351, ATCC TSD-139

straininfo link

  • @ref: 111711
  • straininfo: 401171

literature

  • topic: Phylogeny
  • Pubmed-ID: 31828363
  • title: Acholeplasma equirhinis sp. nov. isolated from respiratory tract of horse (Equus caballus) and Mycoplasma procyoni sp. nov. isolated from oral cavity of raccoon (Procyon lotor).
  • authors: Volokhov DV, Gao Y, Davidson MK, Chizhikov VE
  • journal: Arch Microbiol
  • DOI: 10.1007/s00203-019-01786-x
  • year: 2019
  • mesh: Acholeplasma/classification/genetics/*isolation & purification, Animals, Canada, DNA, Bacterial/genetics, Horses/*microbiology, Mouth/*microbiology, Mycoplasma/classification/genetics/*isolation & purification, Nasopharynx/*microbiology, Phylogeny, RNA, Ribosomal, 16S/genetics, Raccoons/*microbiology, Sequence Analysis, DNA, United Kingdom
  • topic2: Enzymology

Reference

@idauthorstitledoi/urlcataloguejournal
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
66035Curators of the DSMZhttps://www.dsmz.de/collection/catalogue/details/culture/DSM-106692Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 106692)
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
68267Dmitriy V. Volokhov, Yamei Gao, Maureen K. Davidson & Vladimir E. ChizhikovAcholeplasma equirhinis sp. nov. isolated from respiratory tract of horse (Equus caballus) and Mycoplasma procyoni sp. nov. isolated from oral cavity of raccoon (Procyon lotor)10.1007/s00203-019-01786-xArch Microbiol 202: 411-420 2020
111711Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.StrainInfo: A central database for resolving microbial strain identifiers10.60712/SI-ID401171.1