Strain identifier
BacDive ID: 159212
Type strain:
Species: Acholeplasma equirhinis
Strain Designation: N93
Strain history: <- D. V. Volokhov, Dept. LMD, Center for Biologics Evaluation and Research; N93 <- R. M. Lemcke and D. V. Volokhov
NCBI tax ID(s): 555393 (species)
General
@ref: 66035
BacDive-ID: 159212
DSM-Number: 106692
keywords: genome sequence, 16S sequence, Bacteria, facultative anaerobe, mesophilic, colony-forming
description: Acholeplasma equirhinis N93 is a facultative anaerobe, mesophilic bacterium that forms fried-egg-shaped colonies and was isolated from nasopharyngeal swab of a horse.
NCBI tax id
- NCBI tax id: 555393
- Matching level: species
strain history
- @ref: 66035
- history: <- D. V. Volokhov, Dept. LMD, Center for Biologics Evaluation and Research; N93 <- R. M. Lemcke and D. V. Volokhov
doi: 10.13145/bacdive159212.20241212.9.2
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/mycoplasmatota
- domain: Bacteria
- phylum: Mycoplasmatota
- class: Mollicutes
- order: Acholeplasmatales
- family: Acholeplasmataceae
- genus: Acholeplasma
- species: Acholeplasma equirhinis
- full scientific name: Acholeplasma equirhinis Volokhov et al. 2020
@ref: 66035
domain: Bacteria
phylum: Tenericutes
class: Mollicutes
order: Acholeplasmatales
family: Acholeplasmataceae
genus: Acholeplasma
species: Acholeplasma equirhinis
full scientific name: Acholeplasma equirhinis Volokhov et al. 2020
strain designation: N93
type strain: yes
Morphology
cell morphology
- @ref: 69480
- motility: no
- confidence: 90.5
colony morphology
- @ref: 68267
- colony size: 1 mm
- colony shape: fried-egg-shaped
- incubation period: 5-7 days
- medium used: PPLO agar
Culture and growth conditions
culture medium
- @ref: 66035
- name: SP4-Z MEDIUM (DSMZ Medium 1076b)
- growth: yes
- link: https://mediadive.dsmz.de/medium/1076b
- composition: Name: SP4-Z MEDIUM (DSMZ Medium 1076b) Composition: Agar 10.0 g/l Tryptone 10.0 g/l Bacto peptone 5.0 g/l PPLO broth 3.5 g/l Urea 2.0 g/l Glucose 1.0 g/l None 1.0 g/l Yeast extract 0.4 g/l DNA 0.2 g/l CMRL 1066 Fetal bovine serum Swine serum Distilled water
culture temp
@ref | growth | type | temperature |
---|---|---|---|
66035 | positive | growth | 35 |
68267 | positive | growth | 22-41 |
68267 | positive | optimum | 35-37 |
Physiology and metabolism
oxygen tolerance
- @ref: 68267
- oxygen tolerance: facultative anaerobe
observation
@ref | observation |
---|---|
68267 | Bacterial cells lack a cell wall |
68267 | Serum or sterol is required for sustained growth |
metabolite utilization
@ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
---|---|---|---|---|
68267 | 17234 | glucose | + | builds acid from |
68267 | 29016 | arginine | - | hydrolysis |
68267 | 16199 | urea | - | hydrolysis |
antibiotic resistance
- @ref: 68267
- ChEBI: 18208
- metabolite: penicillin g
- is antibiotic: yes
- is resistant: yes
- resistance conc.: 1.5-2.0 g/L
Isolation, sampling and environmental information
isolation
@ref | sample type | country | origin.country | continent | latitude | longitude | enrichment culture | enrichment culture composition | enrichment culture duration | enrichment culture temperature |
---|---|---|---|---|---|---|---|---|---|---|
66035 | nasopharyngeal swab of a horse | United Kingdom | GBR | Europe | 55.3618 | -3.4433 | ||||
68267 | nasopharynx of a thoroughbred horse (Equus caballus) | United Kingdom | GBR | Europe | PPLO broth | containing glucose and penicillin (1.5-2 mg/mL) | 1-2 weeks | 36 |
isolation source categories
Cat1 | Cat2 | Cat3 |
---|---|---|
#Host | #Mammals | #Equidae (Horse) |
#Host Body-Site | #Organ | #Nose |
#Infection | #Patient | #Swab |
Sequence information
16S sequences
@ref | description | accession | length | database | NCBI tax ID |
---|---|---|---|---|---|
66035 | Acholeplasma equirhinis strain N93 16S ribosomal RNA gene and 16S-23S ribosomal RNA intergenic spacer, partial sequence | EU925159 | 1546 | nuccore | 555393 |
66035 | Acholeplasma equirhinis strain N93 23S ribosomal RNA gene, partial sequence; 23S-5S ribosomal RNA intergenic spacer, complete sequence; and 5S ribosomal RNA gene, partial sequence | GU905035 | 2890 | nuccore | 555393 |
66035 | Acholeplasma equirhinis strain N93 RNA polymerase beta subunit gene, partial cds | FJ226562 | 2098 | nuccore | 555393 |
Genome sequences
- @ref: 66792
- description: Acholeplasma equirhinis N93
- accession: GCA_017052655
- assembly level: contig
- database: ncbi
- NCBI tax ID: 555393
GC content
- @ref: 68267
- GC-content: 36.83
- method: high performance liquid chromatography (HPLC)
Genome-based predictions
predictions
@ref | trait | model | description | prediction | confidence | training_data |
---|---|---|---|---|---|---|
69480 | gram-positive | gram-positive | Positive reaction to Gram-staining | yes | 63.44 | no |
69480 | anaerobic | anaerobic | Ability to grow under anoxygenic conditions (including facultative anaerobes) | no | 77.519 | yes |
69480 | aerobic | aerobic | Ability to grow under oxygenic conditions (including facultative aerobes) | no | 92.566 | no |
69480 | spore-forming | spore-forming | Ability to form endo- or exospores | no | 80.412 | no |
69480 | thermophilic | thermophile | Ability to grow at temperatures above or equal to 45°C | no | 94.912 | no |
69480 | flagellated | motile2+ | Ability to perform flagellated movement | no | 90.5 | no |
External links
@ref: 66035
culture collection no.: DSM 106692, NCTC 14351, ATCC TSD-139
straininfo link
- @ref: 111711
- straininfo: 401171
literature
- topic: Phylogeny
- Pubmed-ID: 31828363
- title: Acholeplasma equirhinis sp. nov. isolated from respiratory tract of horse (Equus caballus) and Mycoplasma procyoni sp. nov. isolated from oral cavity of raccoon (Procyon lotor).
- authors: Volokhov DV, Gao Y, Davidson MK, Chizhikov VE
- journal: Arch Microbiol
- DOI: 10.1007/s00203-019-01786-x
- year: 2019
- mesh: Acholeplasma/classification/genetics/*isolation & purification, Animals, Canada, DNA, Bacterial/genetics, Horses/*microbiology, Mouth/*microbiology, Mycoplasma/classification/genetics/*isolation & purification, Nasopharynx/*microbiology, Phylogeny, RNA, Ribosomal, 16S/genetics, Raccoons/*microbiology, Sequence Analysis, DNA, United Kingdom
- topic2: Enzymology
Reference
@id | authors | title | doi/url | catalogue | journal |
---|---|---|---|---|---|
20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | 10.1099/ijsem.0.004332 | ||
66035 | Curators of the DSMZ | https://www.dsmz.de/collection/catalogue/details/culture/DSM-106692 | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 106692) | ||
66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | https://diaspora-project.de/progress.html#genomes | ||
68267 | Dmitriy V. Volokhov, Yamei Gao, Maureen K. Davidson & Vladimir E. Chizhikov | Acholeplasma equirhinis sp. nov. isolated from respiratory tract of horse (Equus caballus) and Mycoplasma procyoni sp. nov. isolated from oral cavity of raccoon (Procyon lotor) | 10.1007/s00203-019-01786-x | Arch Microbiol 202: 411-420 2020 | |
69480 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | https://diaspora-project.de/progress.html#genomes | ||
111711 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | StrainInfo: A central database for resolving microbial strain identifiers | 10.60712/SI-ID401171.1 |