Strain identifier
BacDive ID: 159082
Type strain:
Species: Stieleria maiorica
Strain Designation: Malle 15, Mal15
Strain history: <- M. Jogler, Leibniz-Institut DSMZ GmbH, Braunschweig, Germany; Malle 15 <- A. Stieler
NCBI tax ID(s): 2795974 (species)
General
@ref: 65905
BacDive-ID: 159082
DSM-Number: 100215
keywords: genome sequence, Bacteria, obligate aerobe, heterotroph, mesophilic, Gram-negative
description: Stieleria maiorica Malle 15 is an obligate aerobe, heterotroph, mesophilic bacterium that was isolated from Sand aus Meerwasser.
NCBI tax id
- NCBI tax id: 2795974
- Matching level: species
strain history
- @ref: 65905
- history: <- M. Jogler, Leibniz-Institut DSMZ GmbH, Braunschweig, Germany; Malle 15 <- A. Stieler
doi: 10.13145/bacdive159082.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/planctomycetota
- domain: Bacteria
- phylum: Planctomycetota
- class: Planctomycetia
- order: Pirellulales
- family: Pirellulaceae
- genus: Stieleria
- species: Stieleria maiorica
- full scientific name: Stieleria maiorica Kallscheuer et al. 2021
@ref: 65905
domain: Bacteria
phylum: Planctomycetes
class: Planctomycetia
order: Pirellulales
family: Pirellulaceae
genus: Stieleria
species: Stieleria maiorica
full scientific name: Stieleria maiorica
strain designation: Malle 15, Mal15
type strain: yes
Morphology
cell morphology
@ref | gram stain | confidence | cell length | cell width | cell shape | motility |
---|---|---|---|---|---|---|
69480 | negative | 99.977 | ||||
69660 | variable | 1.7-2.1 µm | 1.2-1.6 µm | other | yes |
colony morphology
- @ref: 69660
- colony color: pink
multimedia
- @ref: 65905
- multimedia content: https://www.dsmz.de/microorganisms/photos/DSM_100215.jpg
- caption: Micrograph of Stieleria maiorica DSM100215, 1000x
- intellectual property rights: Leibniz-Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH
Culture and growth conditions
culture medium
- @ref: 65905
- name: PLANCTOMYCES MEDIUM (DSMZ Medium 1560)
- growth: yes
- link: https://mediadive.dsmz.de/medium/1560
- composition: Name: MARINES PLANCTOMYCES MEDIUM (DSMZ Medium 1560) Composition: Agar 15.0 g/l NaCl 11.735 g/l MgCl2 x 6 H2O 5.32 g/l HEPES 2.38 g/l Na2SO4 1.96 g/l N-Acetylglucosamine 0.8 g/l CaCl2 0.715 g/l MgSO4 x 7 H2O 0.594 g/l KCl 0.345 g/l Glucose 0.25 g/l Peptone 0.25 g/l Yeast extract 0.25 g/l Nitrilotriacetic acid 0.2 g/l NaHCO3 0.095 g/l CaCl2 x 2 H2O 0.0668 g/l KBr 0.048 g/l SrCl2 0.02 g/l H3BO3 0.013 g/l Na-Nitrilotriacetat 0.0015 g/l NaF 0.0015 g/l ZnSO4 x 7 H2O 0.001095 g/l FeSO4 x 7 H2O 0.0005 g/l MnSO4 x H2O 0.0005 g/l Na2MoO7O4 x 2 H2O 0.0002534 g/l Na-EDTA 0.00025 g/l Pyridoxine hydrochloride 0.0001 g/l Fe SO4 x 7 H2O 0.0001 g/l Co(NO3)2 x 6 H2O 0.0001 g/l ZnCl2 0.0001 g/l D-Calcium pantothenate 5e-05 g/l Nicotinamide 5e-05 g/l Thiamine-HCl x 2 H2O 5e-05 g/l Riboflavine 5e-05 g/l p-Aminobenzoic acid 5e-05 g/l NiCl2 x 6 H2O 5e-05 g/l H2SeO3 5e-05 g/l Biotin 2e-05 g/l Folic acid 2e-05 g/l Na2B4O7 x 10 H2O 1.77e-05 g/l CuSO4 x 5 H2O 1e-05 g/l AlK(SO4)2 x 12 H2O 1e-05 g/l Na2MoO4 x 2 H2O 1e-05 g/l Na2WO4 x 2 H2O 1e-05 g/l Vitamin B12 1e-06 g/l Double distilled water Distilled water
culture temp
@ref | growth | type | temperature | range |
---|---|---|---|---|
65905 | positive | growth | 28 | mesophilic |
69660 | positive | growth | 11-37 | |
69660 | positive | optimum | 35 | mesophilic |
culture pH
@ref | ability | type | pH |
---|---|---|---|
69660 | positive | growth | 5.5-9.0 |
69660 | positive | optimum | 7.5 |
Physiology and metabolism
oxygen tolerance
- @ref: 69660
- oxygen tolerance: obligate aerobe
nutrition type
- @ref: 69660
- type: heterotroph
spore formation
@ref | spore formation | confidence |
---|---|---|
69480 | no | 99.991 |
69481 | no | 96 |
observation
- @ref: 69660
- observation: Cells reproduce by polar budding
metabolite utilization
@ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
---|---|---|---|---|
69660 | 17925 | alpha-D-glucose | + | assimilation |
69660 | 36219 | alpha-lactose | + | assimilation |
69660 | 17057 | cellobiose | + | assimilation |
69660 | 15824 | D-fructose | + | assimilation |
69660 | 12936 | D-galactose | + | assimilation |
69660 | 18391 | D-gluconate | + | assimilation |
69660 | 15748 | D-glucuronate | + | assimilation |
69660 | 16024 | D-mannose | + | assimilation |
69660 | 27605 | D-psicose | + | assimilation |
69660 | 16551 | D-trehalose | + | assimilation |
69660 | 28066 | gentiobiose | + | assimilation |
69660 | 32323 | glucuronamide | + | assimilation |
69660 | 30849 | L-arabinose | + | assimilation |
69660 | 18287 | L-fucose | + | assimilation |
69660 | 62345 | L-rhamnose | + | assimilation |
69660 | 6359 | lactulose | + | assimilation |
69660 | 28053 | melibiose | + | assimilation |
69660 | 37657 | methyl D-glucoside | + | assimilation |
69660 | 28037 | n-acetyl-D-galactosamine | + | assimilation |
69660 | 506227 | N-acetyl-D-glucosamine | + | assimilation |
69660 | 16634 | raffinose | + | assimilation |
69660 | 17992 | sucrose | + | assimilation |
69660 | 32528 | turanose | + | assimilation |
69660 | 16810 | 2-oxoglutarate | +/- | assimilation |
69660 | 16411 | acetic acid | +/- | assimilation |
69660 | 15895 | D-galactonic acid lactone | +/- | assimilation |
69660 | 14314 | D-glucose 6-phosphate | +/- | assimilation |
69660 | 16899 | D-mannitol | +/- | assimilation |
69660 | 23652 | dextrin | +/- | assimilation |
69660 | 17306 | maltose | +/- | assimilation |
enzymes
@ref | value | activity | ec |
---|---|---|---|
69660 | cytochrome oxidase | - | 1.9.3.1 |
69660 | catalase | + | 1.11.1.6 |
69660 | alkaline phosphatase | + | 3.1.3.1 |
69660 | esterase (C 4) | + | |
69660 | esterase Lipase (C 8) | + | |
69660 | leucine arylamidase | + | 3.4.11.1 |
69660 | valine arylamidase | + | |
69660 | acid phosphatase | + | 3.1.3.2 |
69660 | naphthol-AS-BI-phosphohydrolase | + |
Isolation, sampling and environmental information
isolation
@ref | sample type | geographic location | country | origin.country | continent |
---|---|---|---|---|---|
65905 | Sand aus Meerwasser | El Arenal, between Balneraio 4 and 5 | Spain | ESP | Europe |
69660 | seawater sediment on Mallorca island | Spain | ESP | Europe |
isolation source categories
Cat1 | Cat2 | Cat3 |
---|---|---|
#Environmental | #Aquatic | #Marine |
#Environmental | #Terrestrial | #Sandy |
Sequence information
Genome sequences
- @ref: 66792
- description: Stieleria maiorica Mal15
- accession: GCA_008035925
- assembly level: chromosome
- database: ncbi
- NCBI tax ID: 2795974
GC content
- @ref: 69660
- GC-content: 59.3
- method: genome sequence analysis
Genome-based predictions
predictions
trait | prediction | confidence | training_data |
---|---|---|---|
spore-forming | no | 96 | no |
motile | yes | 77.496 | no |
flagellated | no | 80.517 | no |
gram-positive | no | 98.087 | no |
anaerobic | no | 96.071 | no |
aerobic | yes | 82.766 | no |
halophile | no | 61.125 | no |
spore-forming | no | 90.879 | no |
thermophile | no | 97.417 | yes |
glucose-util | yes | 88.211 | no |
glucose-ferment | no | 86.366 | no |
External links
@ref: 65905
culture collection no.: DSM 100215, LMG 29790
straininfo link
- @ref: 111592
- straininfo: 399067
literature
topic | Pubmed-ID | title | authors | journal | DOI | year | mesh | topic2 |
---|---|---|---|---|---|---|---|---|
Pathogenicity | 32533057 | The planctomycete Stieleria maiorica Mal15(T) employs stieleriacines to alter the species composition in marine biofilms. | Kallscheuer N, Jeske O, Sandargo B, Boedeker C, Wiegand S, Bartling P, Jogler M, Rohde M, Petersen J, Medema MH, Surup F, Jogler C | Commun Biol | 10.1038/s42003-020-0993-2 | 2020 | Acylation, Anti-Bacterial Agents/chemistry/*pharmacology, Biofilms/drug effects/*growth & development, Gram-Positive Bacteria/drug effects/*growth & development, Planctomycetales/*classification/genetics/isolation & purification/metabolism, Seawater/*microbiology, Tyrosine/chemistry/*pharmacology | Phylogeny |
32868808 | Author Correction: The planctomycete Stieleria maiorica Mal15(T) employs stieleriacines to alter the species composition in marine biofilms. | Kallscheuer N, Jeske O, Sandargo B, Boedeker C, Wiegand S, Bartling P, Jogler M, Rohde M, Petersen J, Medema MH, Surup F, Jogler C | Commun Biol | 10.1038/s42003-020-01228-1 | 2020 | |||
32958819 | Author Correction: The planctomycete Stieleria maiorica Mal15(T) employs stieleriacines to alter the species composition in marine biofilms. | Kallscheuer N, Jeske O, Sandargo B, Boedeker C, Wiegand S, Bartling P, Jogler M, Rohde M, Petersen J, Medema MH, Surup F, Jogler C | Commun Biol | 10.1038/s42003-020-01271-y | 2020 |
Reference
@id | authors | title | doi/url | catalogue |
---|---|---|---|---|
20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | 10.1099/ijsem.0.004332 | |
65905 | Curators of the DSMZ | https://www.dsmz.de/collection/catalogue/details/culture/DSM-100215 | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 100215) | |
66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | https://diaspora-project.de/progress.html#genomes | |
69480 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | https://diaspora-project.de/progress.html#genomes | |
69481 | Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch | Predictions based on the model GenomeNet Sporulation v. 1 | 10.21203/rs.3.rs-2527258/v1 | |
69660 | Nicolai Kallscheuer, Olga Jeske, Birthe Sandargo, Christian Boedeker, Sandra Wiegand, Pascal Bartling, Mareike Jogler, Manfred Rohde, Jörn Petersen, Marnix H. Medema, Frank Surup, Christian Jogler | Author Correction: The planctomycete Stieleria maiorica Mal15T employs stieleriacines to alter the species composition in marine biofilms | 10.1038/s42003-020-01271-y | |
111592 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | StrainInfo: A central database for resolving microbial strain identifiers | 10.60712/SI-ID399067.1 |