Strain identifier

BacDive ID: 159082

Type strain: Yes

Species: Stieleria maiorica

Strain Designation: Malle 15, Mal15

Strain history: <- M. Jogler, Leibniz-Institut DSMZ GmbH, Braunschweig, Germany; Malle 15 <- A. Stieler

NCBI tax ID(s): 2795974 (species)

For citation purpose refer to the digital object identifier (doi) of the current version.
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General

@ref: 65905

BacDive-ID: 159082

DSM-Number: 100215

keywords: genome sequence, Bacteria, obligate aerobe, heterotroph, mesophilic, Gram-negative

description: Stieleria maiorica Malle 15 is an obligate aerobe, heterotroph, mesophilic bacterium that was isolated from Sand aus Meerwasser.

NCBI tax id

  • NCBI tax id: 2795974
  • Matching level: species

strain history

  • @ref: 65905
  • history: <- M. Jogler, Leibniz-Institut DSMZ GmbH, Braunschweig, Germany; Malle 15 <- A. Stieler

doi: 10.13145/bacdive159082.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/planctomycetota
  • domain: Bacteria
  • phylum: Planctomycetota
  • class: Planctomycetia
  • order: Pirellulales
  • family: Pirellulaceae
  • genus: Stieleria
  • species: Stieleria maiorica
  • full scientific name: Stieleria maiorica Kallscheuer et al. 2021

@ref: 65905

domain: Bacteria

phylum: Planctomycetes

class: Planctomycetia

order: Pirellulales

family: Pirellulaceae

genus: Stieleria

species: Stieleria maiorica

full scientific name: Stieleria maiorica

strain designation: Malle 15, Mal15

type strain: yes

Morphology

cell morphology

@refgram stainconfidencecell lengthcell widthcell shapemotility
69480negative99.977
69660variable1.7-2.1 µm1.2-1.6 µmotheryes

colony morphology

  • @ref: 69660
  • colony color: pink

multimedia

  • @ref: 65905
  • multimedia content: https://www.dsmz.de/microorganisms/photos/DSM_100215.jpg
  • caption: Micrograph of Stieleria maiorica DSM100215, 1000x
  • intellectual property rights: Leibniz-Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH

Culture and growth conditions

culture medium

  • @ref: 65905
  • name: PLANCTOMYCES MEDIUM (DSMZ Medium 1560)
  • growth: yes
  • link: https://mediadive.dsmz.de/medium/1560
  • composition: Name: MARINES PLANCTOMYCES MEDIUM (DSMZ Medium 1560) Composition: Agar 15.0 g/l NaCl 11.735 g/l MgCl2 x 6 H2O 5.32 g/l HEPES 2.38 g/l Na2SO4 1.96 g/l N-Acetylglucosamine 0.8 g/l CaCl2 0.715 g/l MgSO4 x 7 H2O 0.594 g/l KCl 0.345 g/l Glucose 0.25 g/l Peptone 0.25 g/l Yeast extract 0.25 g/l Nitrilotriacetic acid 0.2 g/l NaHCO3 0.095 g/l CaCl2 x 2 H2O 0.0668 g/l KBr 0.048 g/l SrCl2 0.02 g/l H3BO3 0.013 g/l Na-Nitrilotriacetat 0.0015 g/l NaF 0.0015 g/l ZnSO4 x 7 H2O 0.001095 g/l FeSO4 x 7 H2O 0.0005 g/l MnSO4 x H2O 0.0005 g/l Na2MoO7O4 x 2 H2O 0.0002534 g/l Na-EDTA 0.00025 g/l Pyridoxine hydrochloride 0.0001 g/l Fe SO4 x 7 H2O 0.0001 g/l Co(NO3)2 x 6 H2O 0.0001 g/l ZnCl2 0.0001 g/l D-Calcium pantothenate 5e-05 g/l Nicotinamide 5e-05 g/l Thiamine-HCl x 2 H2O 5e-05 g/l Riboflavine 5e-05 g/l p-Aminobenzoic acid 5e-05 g/l NiCl2 x 6 H2O 5e-05 g/l H2SeO3 5e-05 g/l Biotin 2e-05 g/l Folic acid 2e-05 g/l Na2B4O7 x 10 H2O 1.77e-05 g/l CuSO4 x 5 H2O 1e-05 g/l AlK(SO4)2 x 12 H2O 1e-05 g/l Na2MoO4 x 2 H2O 1e-05 g/l Na2WO4 x 2 H2O 1e-05 g/l Vitamin B12 1e-06 g/l Double distilled water Distilled water

culture temp

@refgrowthtypetemperaturerange
65905positivegrowth28mesophilic
69660positivegrowth11-37
69660positiveoptimum35mesophilic

culture pH

@refabilitytypepH
69660positivegrowth5.5-9.0
69660positiveoptimum7.5

Physiology and metabolism

oxygen tolerance

  • @ref: 69660
  • oxygen tolerance: obligate aerobe

nutrition type

  • @ref: 69660
  • type: heterotroph

spore formation

@refspore formationconfidence
69480no99.991
69481no96

observation

  • @ref: 69660
  • observation: Cells reproduce by polar budding

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6966017925alpha-D-glucose+assimilation
6966036219alpha-lactose+assimilation
6966017057cellobiose+assimilation
6966015824D-fructose+assimilation
6966012936D-galactose+assimilation
6966018391D-gluconate+assimilation
6966015748D-glucuronate+assimilation
6966016024D-mannose+assimilation
6966027605D-psicose+assimilation
6966016551D-trehalose+assimilation
6966028066gentiobiose+assimilation
6966032323glucuronamide+assimilation
6966030849L-arabinose+assimilation
6966018287L-fucose+assimilation
6966062345L-rhamnose+assimilation
696606359lactulose+assimilation
6966028053melibiose+assimilation
6966037657methyl D-glucoside+assimilation
6966028037n-acetyl-D-galactosamine+assimilation
69660506227N-acetyl-D-glucosamine+assimilation
6966016634raffinose+assimilation
6966017992sucrose+assimilation
6966032528turanose+assimilation
69660168102-oxoglutarate+/-assimilation
6966016411acetic acid+/-assimilation
6966015895D-galactonic acid lactone+/-assimilation
6966014314D-glucose 6-phosphate+/-assimilation
6966016899D-mannitol+/-assimilation
6966023652dextrin+/-assimilation
6966017306maltose+/-assimilation

enzymes

@refvalueactivityec
69660cytochrome oxidase-1.9.3.1
69660catalase+1.11.1.6
69660alkaline phosphatase+3.1.3.1
69660esterase (C 4)+
69660esterase Lipase (C 8)+
69660leucine arylamidase+3.4.11.1
69660valine arylamidase+
69660acid phosphatase+3.1.3.2
69660naphthol-AS-BI-phosphohydrolase+

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinent
65905Sand aus MeerwasserEl Arenal, between Balneraio 4 and 5SpainESPEurope
69660seawater sediment on Mallorca islandSpainESPEurope

isolation source categories

Cat1Cat2Cat3
#Environmental#Aquatic#Marine
#Environmental#Terrestrial#Sandy

Sequence information

Genome sequences

  • @ref: 66792
  • description: Stieleria maiorica Mal15
  • accession: GCA_008035925
  • assembly level: chromosome
  • database: ncbi
  • NCBI tax ID: 2795974

GC content

  • @ref: 69660
  • GC-content: 59.3
  • method: genome sequence analysis

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno96no
motileyes77.496no
flagellatedno80.517no
gram-positiveno98.087no
anaerobicno96.071no
aerobicyes82.766no
halophileno61.125no
spore-formingno90.879no
thermophileno97.417yes
glucose-utilyes88.211no
glucose-fermentno86.366no

External links

@ref: 65905

culture collection no.: DSM 100215, LMG 29790

straininfo link

  • @ref: 111592
  • straininfo: 399067

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Pathogenicity32533057The planctomycete Stieleria maiorica Mal15(T) employs stieleriacines to alter the species composition in marine biofilms.Kallscheuer N, Jeske O, Sandargo B, Boedeker C, Wiegand S, Bartling P, Jogler M, Rohde M, Petersen J, Medema MH, Surup F, Jogler CCommun Biol10.1038/s42003-020-0993-22020Acylation, Anti-Bacterial Agents/chemistry/*pharmacology, Biofilms/drug effects/*growth & development, Gram-Positive Bacteria/drug effects/*growth & development, Planctomycetales/*classification/genetics/isolation & purification/metabolism, Seawater/*microbiology, Tyrosine/chemistry/*pharmacologyPhylogeny
32868808Author Correction: The planctomycete Stieleria maiorica Mal15(T) employs stieleriacines to alter the species composition in marine biofilms.Kallscheuer N, Jeske O, Sandargo B, Boedeker C, Wiegand S, Bartling P, Jogler M, Rohde M, Petersen J, Medema MH, Surup F, Jogler CCommun Biol10.1038/s42003-020-01228-12020
32958819Author Correction: The planctomycete Stieleria maiorica Mal15(T) employs stieleriacines to alter the species composition in marine biofilms.Kallscheuer N, Jeske O, Sandargo B, Boedeker C, Wiegand S, Bartling P, Jogler M, Rohde M, Petersen J, Medema MH, Surup F, Jogler CCommun Biol10.1038/s42003-020-01271-y2020

Reference

@idauthorstitledoi/urlcatalogue
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
65905Curators of the DSMZhttps://www.dsmz.de/collection/catalogue/details/culture/DSM-100215Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 100215)
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. MünchPredictions based on the model GenomeNet Sporulation v. 110.21203/rs.3.rs-2527258/v1
69660Nicolai Kallscheuer, Olga Jeske, Birthe Sandargo, Christian Boedeker, Sandra Wiegand, Pascal Bartling, Mareike Jogler, Manfred Rohde, Jörn Petersen, Marnix H. Medema, Frank Surup, Christian JoglerAuthor Correction: The planctomycete Stieleria maiorica Mal15T employs stieleriacines to alter the species composition in marine biofilms10.1038/s42003-020-01271-y
111592Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.StrainInfo: A central database for resolving microbial strain identifiers10.60712/SI-ID399067.1