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Strain identifier

BacDive ID: 159052

Type strain: Yes

Species: Dysosmobacter welbionis

Strain Designation: J115

Strain history: <- P. Cani, Catholic University of Louvain, Unit of Bioengineering, Louvain-la Neuve, Belgium; J115 <- T. Le Roy {2017}

NCBI tax ID(s): 2093857 (species)

For citation purpose refer to the digital object identifier (doi) of the current version.
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General

@ref: 65875

BacDive-ID: 159052

DSM-Number: 106889

keywords: genome sequence, 16S sequence, Bacteria, facultative anaerobe, mesophilic, Gram-negative, rod-shaped, colony-forming

description: Dysosmobacter welbionis J115 is a facultative anaerobe, mesophilic, Gram-negative bacterium that forms punctiform colonies and was isolated from healthy human stool.

NCBI tax id

  • NCBI tax id: 2093857
  • Matching level: species

strain history: <- P. Cani, Catholic University of Louvain, Unit of Bioengineering, Louvain-la Neuve, Belgium; J115 <- T. Le Roy {2017}

doi: 10.13145/bacdive159052.20221219.7.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Clostridia
  • order: Eubacteriales
  • family: Oscillospiraceae
  • genus: Dysosmobacter
  • species: Dysosmobacter welbionis
  • full scientific name: Dysosmobacter welbionis Le Roy et al. 2020

@ref: 65875

domain: Bacteria

phylum: Firmicutes

class: Clostridia

order: Eubacteriales

family: Oscillospiraceae

genus: Dysosmobacter

species: Dysosmobacter welbionis

full scientific name: Dysosmobacter welbionis Le Roy et al. 2020

strain designation: J115

type strain: yes

Morphology

cell morphology

@refgram staincell lengthcell shapemotilityconfidence
68170negative1.8-3.0 µmrod-shapedno
69480positive36.576

colony morphology

  • @ref: 68170
  • colony color: cream
  • colony shape: punctiform
  • incubation period: 3 days
  • medium used: modified YCFA

pigmentation

  • @ref: 68170
  • production: no

Culture and growth conditions

culture medium

  • @ref: 65875
  • name: PY + X MEDIUM (N2/CO2) (DSMZ Medium 104c)
  • growth: yes
  • link: https://mediadive.dsmz.de/medium/104c
  • composition: Name: PY + X MEDIUM (N2/CO2) (DSMZ Medium 104c) Composition: Yeast extract 10.0 g/l D-Glucose 5.0 g/l Trypticase peptone 5.0 g/l Meat peptone 5.0 g/l Na2CO3 1.0 g/l L-Cysteine HCl x H2O 0.5 g/l NaHCO3 0.4 g/l NaCl 0.08 g/l KH2PO4 0.04 g/l K2HPO4 0.04 g/l MgSO4 x 7 H2O 0.02 g/l CaCl2 x 2 H2O 0.01 g/l Sodium resazurin 0.0005 g/l Distilled water

culture temp

  • @ref: 65875
  • growth: positive
  • type: growth
  • temperature: 37
  • range: mesophilic

Physiology and metabolism

tolerance

  • @ref: 68170
  • compound: Bile
  • percentage: 0-2

oxygen tolerance

@refoxygen toleranceconfidence
68170facultative anaerobe
69480anaerobe96.465

spore formation

@refspore formationconfidence
68170no
69480no96.234

halophily

@refsaltgrowthtested relationconcentrationhalophily levelconfidence
68170NaClnogrowth2 %(w/v)
68170NaClpositivegrowth0-1.4 %(w/v)
69480non-halophilic98.389

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
681704853esculin+hydrolysis
6817017632nitrate-reduction
681705291gelatin-hydrolysis
6817017268myo-inositol+builds acid from
6817015963ribitol-builds acid from
6817027613amygdalin-builds acid from
6817017108D-arabinose-builds acid from
6817030849L-arabinose-builds acid from
6817018333D-arabitol-builds acid from
6817018403L-arabitol-builds acid from
6817018305arbutin-builds acid from
6817017057cellobiose-builds acid from
6817016813galactitol-builds acid from
6817017113erythritol-builds acid from
6817015824D-fructose-builds acid from
6817028847D-fucose-builds acid from
6817018287L-fucose-builds acid from
6817012936D-galactose-builds acid from
6817028066gentiobiose-builds acid from
6817017634D-glucose-builds acid from
6817017754glycerol-builds acid from
6817028087glycogen-builds acid from
6817015443inulin-builds acid from
6817017716lactose-builds acid from
6817025097lyxose-builds acid from
6817017306maltose-builds acid from
6817016899D-mannitol-builds acid from
6817016024D-mannose-builds acid from
6817028053melibiose-builds acid from
68170320061methyl alpha-D-glucopyranoside-builds acid from
6817043943methyl alpha-D-mannoside-builds acid from
6817074863methyl beta-D-xylopyranoside-builds acid from
6817059640N-acetylglucosamine-builds acid from
6817016634raffinose-builds acid from
6817062345L-rhamnose-builds acid from
6817016988D-ribose-builds acid from
6817017992sucrose-builds acid from
6817017814salicin-builds acid from
6817017924D-sorbitol-builds acid from
6817017266L-sorbose-builds acid from
6817028017starch-builds acid from
6817033954tagatose-builds acid from
6817027082trehalose-builds acid from
6817032528turanose-builds acid from
6817017151xylitol-builds acid from
6817065327D-xylose-builds acid from
6817065328L-xylose-builds acid from

metabolite production

  • @ref: 68170
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

enzymes

@refvalueactivityec
68170urease-3.5.1.5
68170catalase-1.11.1.6
68170arginine dihydrolase+3.5.3.6
68170glutamate decarboxylase+4.1.1.15
68170alkaline phosphatase-3.1.3.1
68170alpha-galactosidase-3.2.1.22
68170beta-galactosidase-3.2.1.23
68170alpha-glucosidase-3.2.1.20
68170beta-glucosidase-3.2.1.21
68170alpha-arabinosidase-
68170beta-glucuronidase-3.2.1.31
68170N-acetyl-beta-glucosaminidase-3.2.1.52
68170alpha-fucosidase-3.2.1.51
68170arginine arylamidase-
68170proline-arylamidase-3.4.11.5
68170leucyl glycin arylamidase-3.4.11.1
68170phenylalanine arylamidase-
68170pyroglutamic acid arylamidase-
68170tyrosine arylamidase-
68170alanine arylamidase-3.4.11.2
68170glycin arylamidase-
68170histidine arylamidase-
68170serine arylamidase-

fatty acid profile

  • fatty acids

    @reffatty acidpercentage
    68170C14:02.4
    68170C15:0 anteiso-DMA1
    68170C16:0 DMA7.6
    68170C17:0 DMA2.2
    68170C18:0 DMA18.4
    68170C14:0 iso1.5
    68170C15:0 iso24.2
    68170C15:0 anteiso15.2
  • type of FA analysis: whole cell analysis
  • incubation medium: modified YCFA
  • system: MIS MIDI
  • method/protocol: Kuykendall et al. 1988
  • cutoff value:

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentlatitudelongitudeenrichment cultureenrichment culture durationenrichment culture temperatureisolation date
65875healthy human stoolBruxellesBelgiumBELEurope
68170faecal sample from a healthy 25 year old female humanBrusselsBelgiumBELEurope50.85154.45556modified YCFA2-7 days372017-07-01

isolation source categories

Cat1Cat2Cat3
#Host Body Product#Gastrointestinal tract#Feces (Stool)
#Host#Human

taxonmaps

  • @ref: 69479
  • File name: preview.99_1451.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_91;96_833;97_972;98_1145;99_1451&stattab=map
  • Last taxonomy: Dysosmobacter welbionis subclade
  • 16S sequence: MG963288
  • Sequence Identity:
  • Total samples: 193822
  • soil counts: 976
  • aquatic counts: 2321
  • animal counts: 190177
  • plant counts: 348

Sequence information

16S sequences

  • @ref: 68170
  • description: Dysosmobacter welbionis strain J115 16S ribosomal RNA gene, partial sequence
  • accession: MG963288
  • length: 1428
  • database: ena
  • NCBI tax ID: 2093857

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Oscillospiraceae bacterium J1152093857.4completepatric2093857
68170Dysosmobacter welbionis J115GCA_005121165completencbi2093857
66792Listeria monocytogenes A504GCA_001710935contigpatric1639

GC content

@refGC-contentmethod
6587558.9sequence analysis
6817059.3high performance liquid chromatography (HPLC)
6817058.92genome sequence analysis

External links

@ref: 65875

culture collection no.: DSM 106889, LMG 30601

literature

  • topic: Phylogeny
  • Pubmed-ID: 31232680
  • title: Dysosmobacter welbionis gen. nov., sp. nov., isolated from human faeces and emended description of the genus Oscillibacter.
  • authors: Le Roy T, Van der Smissen P, Paquot A, Delzenne N, Muccioli GG, Collet JF, Cani PD
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijsem.0.003547
  • year: 2020
  • mesh: Adult, Bacterial Typing Techniques, Base Composition, Belgium, Clostridiales/*classification/isolation & purification, DNA, Bacterial/genetics, Fatty Acids/chemistry, Feces/*microbiology, Female, Humans, Nucleic Acid Hybridization, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA
  • topic2: Pathogenicity

Reference

@idauthorstitledoi/urlcataloguejournal
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
65875Curators of the DSMZhttps://www.dsmz.de/collection/catalogue/details/culture/DSM-106889Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 106889)
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
68170Tiphaine Le Roy, Patrick Van der Smissen, Adrien Paquot, Nathalie Delzenne, Giulio G. Muccioli, Jean-François Collet and Patrice D. CaniDysosmobacter welbionis gen. nov., sp. nov., isolated from human faeces and emended description of the genus Oscillibacter10.1099/ijsem.0.003547IJSEM 70: 4851-4858 2020
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.MicrobeAtlas 1.0 betahttps://microbeatlas.org/
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes