Strain identifier

BacDive ID: 158904

Type strain: Yes

Species: Eisenbergiella porci

Strain Designation: WCA-389-WT-23B

Strain history: B. Abt; DSMZ, Germany; DSM 101007 (=WCA-389-WT-23B) <-- T. Clavel; RWTH Aachen, Germany.

NCBI tax ID(s): 2652274 (species)

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General

@ref: 65726

BacDive-ID: 158904

DSM-Number: 101007

keywords: genome sequence, 16S sequence, Bacteria, mesophilic

description: Eisenbergiella porci WCA-389-WT-23B is a mesophilic bacterium that was isolated from feces; pig, 8 weeks old, wild type.

NCBI tax id

  • NCBI tax id: 2652274
  • Matching level: species

strain history

@refhistory
65726<- T. Clavel , TU Munich, Freising-Weihenstephan, Germany; WCA-389-WT-23B
67770B. Abt; DSMZ, Germany; DSM 101007 (=WCA-389-WT-23B) <-- T. Clavel; RWTH Aachen, Germany.

doi: 10.13145/bacdive158904.20250331.9.3

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Clostridia
  • order: Eubacteriales
  • family: Lachnospiraceae
  • genus: Eisenbergiella
  • species: Eisenbergiella porci
  • full scientific name: Eisenbergiella porci Wylensek et al. 2021

@ref: 65726

domain: Bacteria

phylum: Firmicutes

class: Clostridia

order: Eubacteriales

family: Lachnospiraceae

genus: Eisenbergiella

species: Eisenbergiella porci

full scientific name: Eisenbergiella porci

strain designation: WCA-389-WT-23B

type strain: yes

Culture and growth conditions

culture medium

  • @ref: 65726
  • name: PYG MEDIUM (MODIFIED) (DSMZ Medium 104)
  • growth: yes
  • link: https://mediadive.dsmz.de/medium/104
  • composition: Name: PYG MEDIUM (modified) (DSMZ Medium 104) Composition: Yeast extract 10.0 g/l Peptone 5.0 g/l Trypticase peptone 5.0 g/l Beef extract 5.0 g/l Glucose 5.0 g/l L-Cysteine HCl x H2O 0.5 g/l NaHCO3 0.4 g/l NaCl 0.08 g/l K2HPO4 0.04 g/l KH2PO4 0.04 g/l MgSO4 x 7 H2O 0.02 g/l CaCl2 x 2 H2O 0.01 g/l Hemin 0.005 g/l Ethanol 0.0038 g/l Resazurin 0.001 g/l Tween 80 Vitamin K1 NaOH Distilled water

culture temp

@refgrowthtypetemperature
65726positivegrowth37
67770positivegrowth37

Physiology and metabolism

oxygen tolerance

  • @ref: 125439
  • oxygen tolerance: anaerobe
  • confidence: 99.8

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6838027897tryptophan-energy source
6838017632nitrate-reduction
6838029985L-glutamate-degradation
6838016634raffinose-fermentation
6838029016arginine-hydrolysis
6838016024D-mannose-fermentation
6838016199urea-hydrolysis

metabolite production

  • @ref: 68380
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

metabolite tests

  • @ref: 68380
  • Chebi-ID: 35581
  • metabolite: indole
  • indole test: -

enzymes

@refvalueactivityec
68380serine arylamidase-
68380glycin arylamidase-
68380alanine arylamidase-3.4.11.2
68380tyrosine arylamidase-
68380phenylalanine arylamidase-
68380leucine arylamidase-3.4.11.1
68380proline-arylamidase-3.4.11.5
68380leucyl glycin arylamidase-3.4.11.1
68380L-arginine arylamidase-
68380alkaline phosphatase-3.1.3.1
68380tryptophan deaminase-4.1.99.1
68380alpha-fucosidase-3.2.1.51
68380N-acetyl-beta-glucosaminidase+3.2.1.52
68380beta-glucuronidase-3.2.1.31
68380beta-glucosidase+3.2.1.21
68380beta-galactosidase+3.2.1.23
68380beta-Galactosidase 6-phosphate-
68380arginine dihydrolase-3.5.3.6
68380urease-3.5.1.5
68380glutamyl-glutamate arylamidase-
68380histidine arylamidase-
68380pyrrolidonyl arylamidase-3.4.19.3
68380glutamate decarboxylase-4.1.1.15
68380alpha-arabinosidase+3.2.1.55
68380alpha-glucosidase+3.2.1.20
68380alpha-galactosidase+3.2.1.22

API rID32A

@refUREADH Argalpha GALbeta GALbeta GPalpha GLUbeta GLUalpha ARAbeta GURbeta NAGMNERAFGDCalpha FUCNITINDPALArgAProALGAPheALeuAPyrATyrAAlaAGlyAHisAGGASerA
65726--++-+++-+-------------------

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinent
65726feces; pig, 8 weeks old, wild typeKranzberg (Bavaria)GermanyDEUEurope
67770Feces of a German Landrace pigFreisingGermanyDEUEurope

isolation source categories

Cat1Cat2Cat3
#Host Body Product#Gastrointestinal tract#Feces (Stool)
#Host#Mammals#Suidae (Pig,Swine)

taxonmaps

  • @ref: 69479
  • File name: preview.99_1452.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_16;96_834;97_973;98_1146;99_1452&stattab=map
  • Last taxonomy: Eisenbergiella massiliensis subclade
  • 16S sequence: MN537455
  • Sequence Identity:
  • Total samples: 8934
  • soil counts: 23
  • aquatic counts: 86
  • animal counts: 8792
  • plant counts: 33

Safety information

risk assessment

  • @ref: 65726
  • pathogenicity human: yes, in single cases
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 65726
  • description: Eisenbergiella porci strain WCA-389-WT-23B 16S ribosomal RNA gene, partial sequence
  • accession: MN537455
  • length: 1432
  • database: nuccore
  • NCBI tax ID: 2652274

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Eisenbergiella porci WCA-389-WT-23BGCA_009696275contigncbi2652274
66792Eisenbergiella tayi strain WCA-389-WT-23B1432052.291wgspatric1432052

GC content

  • @ref: 67770
  • GC-content: 48.4
  • method: genome sequence analysis

Genome-based predictions

predictions

@refmodeltraitdescriptionpredictionconfidencetraining_data
125438gram-positivegram-positivePositive reaction to Gram-stainingyes70.675no
125438anaerobicanaerobicAbility to grow under anoxygenic conditions (including facultative anaerobes)yes83.045no
125438spore-formingspore-formingAbility to form endo- or exosporesyes60.099no
125438aerobicaerobicAbility to grow under oxygenic conditions (including facultative aerobes)no90.32no
125438thermophilethermophilicAbility to grow at temperatures above or equal to 45°Cno92.708yes
125438motile2+flagellatedAbility to perform flagellated movementno72.93no
125439BacteriaNetspore_formationAbility to form endo- or exosporesno53.9
125439BacteriaNetmotilityAbility to perform movementyes55.4
125439BacteriaNetgram_stainReaction to gram-stainingpositive71
125439BacteriaNetoxygen_toleranceOxygenic conditions needed for growthanaerobe99.8

External links

@ref: 65726

culture collection no.: DSM 101007, JCM 34365

straininfo link

  • @ref: 111423
  • straininfo: 404459

Reference

@idauthorstitledoi/urlcatalogue
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
65726Curators of the DSMZhttps://www.dsmz.de/collection/catalogue/details/culture/DSM-101007Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 101007)
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68380Automatically annotated from API rID32A
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.MicrobeAtlas 1.0 betahttps://microbeatlas.org/
111423Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.StrainInfo: A central database for resolving microbial strain identifiers10.60712/SI-ID404459.1
125438Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg OvermannPredicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets10.1101/2024.08.12.607695
125439Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardydeepG: Deep Learning for Genome Sequence Data. R package version 0.3.1https://github.com/GenomeNet/deepG