Strain identifier
BacDive ID: 158819
Type strain:
Species: Flavobacterium alvei
Strain Designation: HR-AY
Strain history: C. O. Jeon; Chung-Ang Univ., South Korea; HR-AY.
NCBI tax ID(s): 2080416 (species)
General
@ref: 65583
BacDive-ID: 158819
keywords: genome sequence, 16S sequence, Bacteria, obligate aerobe, Gram-negative, rod-shaped, colony-forming
description: Flavobacterium alvei HR-AY is an obligate aerobe, Gram-negative, rod-shaped bacterium that forms circular colonies and was isolated from freshwater river.
NCBI tax id
- NCBI tax id: 2080416
- Matching level: species
strain history
- @ref: 67770
- history: C. O. Jeon; Chung-Ang Univ., South Korea; HR-AY.
doi: 10.13145/bacdive158819.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/bacteroidota
- domain: Bacteria
- phylum: Bacteroidota
- class: Flavobacteriia
- order: Flavobacteriales
- family: Flavobacteriaceae
- genus: Flavobacterium
- species: Flavobacterium alvei
- full scientific name: Flavobacterium alvei Lee and Jeon 2018
@ref: 65583
domain: Bacteria
phylum: Bacteroidetes
class: Flavobacteriia
order: Flavobacteriales
family: Flavobacteriaceae
genus: Flavobacterium
species: Flavobacterium alvei
strain designation: HR-AY
type strain: yes
Morphology
cell morphology
- @ref: 65583
- gram stain: negative
- cell length: 1.0-1.3 µm
- cell width: 0.4-0.5 µm
- cell shape: rod-shaped
- motility: no
colony morphology
- @ref: 65583
- colony color: yellow
- colony shape: circular
- medium used: R2A
pigmentation
- @ref: 65583
- production: no
- name: flexirubin-type
Culture and growth conditions
culture medium
- @ref: 65583
- name: Reasoner's 2A agar (R2A)
- growth: yes
culture temp
@ref | growth | type | temperature | range |
---|---|---|---|---|
65583 | positive | growth | 5-30 | |
65583 | positive | optimum | 20 | psychrophilic |
67770 | positive | growth | 20 | psychrophilic |
culture pH
@ref | ability | type | pH | PH range |
---|---|---|---|---|
65583 | positive | growth | 5.0-9.0 | alkaliphile |
65583 | positive | optimum | 7 |
Physiology and metabolism
oxygen tolerance
- @ref: 65583
- oxygen tolerance: obligate aerobe
halophily
- @ref: 65583
- salt: NaCl
- growth: positive
- tested relation: growth
- concentration: 0 %
observation
- @ref: 67770
- observation: quinones: MK-6
metabolite utilization
@ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
---|---|---|---|---|
65583 | 17128 | adipate | - | assimilation |
65583 | 27689 | decanoate | - | assimilation |
65583 | casein | - | hydrolysis | |
65583 | 17634 | D-glucose | + | assimilation |
65583 | 16899 | D-mannitol | - | assimilation |
65583 | 16024 | D-mannose | + | assimilation |
65583 | 4853 | esculin | + | hydrolysis |
65583 | 5291 | gelatin | + | hydrolysis |
65583 | 17234 | glucose | + | fermentation |
65583 | 30849 | L-arabinose | + | assimilation |
65583 | 25115 | malate | - | assimilation |
65583 | 17306 | maltose | + | assimilation |
65583 | 506227 | N-acetylglucosamine | + | assimilation |
65583 | 18401 | phenylacetate | - | assimilation |
65583 | 32032 | potassium gluconate | - | assimilation |
65583 | 53258 | sodium citrate | - | assimilation |
65583 | 28017 | starch | - | hydrolysis |
65583 | 53424 | tween 20 | - | hydrolysis |
65583 | 53426 | tween 80 | - | hydrolysis |
65583 | 18186 | tyrosine | - | hydrolysis |
metabolite production
@ref | Chebi-ID | metabolite | production |
---|---|---|---|
65583 | 17997 | dinitrogen | no |
65583 | 35581 | indole | no |
enzymes
@ref | value | activity | ec |
---|---|---|---|
65583 | catalase | - | 1.11.1.6 |
65583 | cytochrome oxidase | - | 1.9.3.1 |
65583 | alkaline phosphatase | + | 3.1.3.1 |
65583 | esterase (C 4) | + | |
65583 | esterase Lipase (C 8) | + | |
65583 | leucine arylamidase | + | 3.4.11.1 |
65583 | acid phosphatase | + | 3.1.3.2 |
65583 | naphthol-AS-BI-phosphohydrolase | + | |
65583 | beta-galactosidase | + | 3.2.1.23 |
65583 | beta-galactopyranosidase | + | |
65583 | arginine dihydrolase | + | 3.5.3.6 |
65583 | N-acetyl-beta-glucosaminidase | + | 3.2.1.52 |
65583 | trypsin | - | 3.4.21.4 |
65583 | alpha-galactosidase | - | 3.2.1.22 |
65583 | alpha-fucosidase | - | 3.2.1.51 |
65583 | lipase (C 14) | - | |
65583 | alpha-chymotrypsin | - | 3.4.21.1 |
65583 | alpha-mannosidase | - | 3.2.1.24 |
65583 | urease | - | 3.5.1.5 |
65583 | cystine arylamidase | - | 3.4.11.3 |
65583 | beta-glucuronidase | - | 3.2.1.31 |
65583 | alpha-glucosidase | - | 3.2.1.20 |
65583 | valine arylamidase | - |
fatty acid profile
fatty acids
@ref fatty acid percentage 65583 C12:0 1.5 65583 C16:0 5.8 65583 C13:0 iso 1.8 65583 C14:0 iso 1.4 65583 C15:0 iso 13.3 65583 C13:0 anteiso 1.6 65583 C15:0 anteiso 15.3 65583 C15:0 2OH 1 65583 C16:0 3OH 4.5 65583 C17:0 2OH 1.3 65583 C15:0 iso 3OH 3.7 65583 C17:0 iso 3OH 3.3 65583 C13:1 1.6 65583 C15:1 iso G 1.2 65583 C15:1 anteiso A 1.2 65583 C17:1 anteiso ω9c 1.4 65583 C14:0 3OH / C16:1 iso I 1.3 65583 C16:1ω7c / C16:1ω6c 27.2 65583 C18:1ω7c / C18:1ω6c 1.3 65583 10-methyl C16:0 / iso-C17:1ω9c 1.1 - type of FA analysis: whole cell analysis
- incubation medium: R2A
- incubation temperature: 20
- software version: Sherlock 6.2B
- library/peak naming table: TSBA6
- system: MIS MIDI
- method/protocol: Sasser, 1990
- cutoff value:
Isolation, sampling and environmental information
isolation
@ref | sample type | geographic location | country | origin.country | continent | latitude | longitude | enrichment culture | enrichment culture duration | enrichment culture temperature | isolation procedure |
---|---|---|---|---|---|---|---|---|---|---|---|
65583 | freshwater river | Han River, Seoul | Republic of Korea | KOR | Asia | 37.5094 | 126.965 | R2A | 5 days | 25 | sample was serially diluted in phosphate-buffered saline (PBS) and 100 µl of each dilution was spread on R2A |
67770 | Water sample of the Han River | Republic of Korea | KOR | Asia |
isolation source categories
Cat1 | Cat2 | Cat3 |
---|---|---|
#Environmental | #Aquatic | #Freshwater |
#Environmental | #Aquatic | #River (Creek) |
Sequence information
16S sequences
- @ref: 65583
- description: 16S rRNA gene sequence
- accession: MF685248
- database: nuccore
Genome sequences
- @ref: 67770
- description: Flavobacterium alvei HR-AY
- accession: GCA_002920895
- assembly level: contig
- database: ncbi
- NCBI tax ID: 2080416
GC content
- @ref: 67770
- GC-content: 34.4
- method: genome sequence analysis
Genome-based predictions
predictions
trait | prediction | confidence | training_data |
---|---|---|---|
flagellated | no | 96.605 | no |
gram-positive | no | 98.273 | yes |
anaerobic | no | 98.869 | yes |
aerobic | yes | 84.644 | yes |
halophile | no | 98.384 | yes |
spore-forming | no | 95.476 | no |
glucose-util | yes | 90.288 | yes |
thermophile | no | 98.003 | no |
motile | no | 92.53 | yes |
glucose-ferment | no | 83.264 | yes |
External links
@ref: 65583
culture collection no.: KACC 19407, JCM 32264
literature
topic | Pubmed-ID | title | authors | journal | DOI | year | mesh | topic2 |
---|---|---|---|---|---|---|---|---|
Phylogeny | 29676721 | Flavobacterium alvei sp. nov., isolated from a freshwater river. | Lee Y, Jeon CO | Int J Syst Evol Microbiol | 10.1099/ijsem.0.002768 | 2018 | Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Fatty Acids/chemistry, Flavobacterium/*classification/genetics/isolation & purification, Phosphatidylethanolamines/chemistry, *Phylogeny, Pigmentation, RNA, Ribosomal, 16S/genetics, Republic of Korea, Rivers/*microbiology, Sequence Analysis, DNA, Vitamin K 2/analogs & derivatives/chemistry | Transcriptome |
Phylogeny | 33108487 | Flavobacterium undicola sp. nov., isolated from a freshwater lake. | Chen WM, Xie YR, Kwon SW, Sheu SY | Arch Microbiol | 10.1007/s00203-020-02096-3 | 2020 | Base Composition, DNA, Bacterial/genetics, Fatty Acids/chemistry, Flavobacterium/*classification/genetics, Lakes/*microbiology, Nucleic Acid Hybridization, Phospholipids/chemistry, *Phylogeny, Polyamines, RNA, Ribosomal, 16S/genetics, Species Specificity, Taiwan |
Reference
@id | authors | title | doi/url | journal | pubmed |
---|---|---|---|---|---|
20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | 10.1099/ijsem.0.004332 | ||
65583 | Yunho Lee and Che Ok Jeon | Flavobacterium alvei sp. nov., isolated from a freshwater river | 10.1099/ijsem.0.002768 | IJSEM 68: 1919-1924 2018 | 29676721 |
66794 | Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg | BRENDA, the ELIXIR core data resource in 2021: new developments and updates | 10.1093/nar/gkaa1025 | Nucleic Acids Res. 49: D498-D508 2020 | 33211880 |
67770 | Curators of the JCM | https://jcm.brc.riken.jp/en/ |