Strain identifier

BacDive ID: 158819

Type strain: Yes

Species: Flavobacterium alvei

Strain Designation: HR-AY

Strain history: C. O. Jeon; Chung-Ang Univ., South Korea; HR-AY.

NCBI tax ID(s): 2080416 (species)

For citation purpose refer to the digital object identifier (doi) of the current version.
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General

@ref: 65583

BacDive-ID: 158819

keywords: genome sequence, 16S sequence, Bacteria, obligate aerobe, Gram-negative, rod-shaped, colony-forming

description: Flavobacterium alvei HR-AY is an obligate aerobe, Gram-negative, rod-shaped bacterium that forms circular colonies and was isolated from freshwater river.

NCBI tax id

  • NCBI tax id: 2080416
  • Matching level: species

strain history

  • @ref: 67770
  • history: C. O. Jeon; Chung-Ang Univ., South Korea; HR-AY.

doi: 10.13145/bacdive158819.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacteroidota
  • domain: Bacteria
  • phylum: Bacteroidota
  • class: Flavobacteriia
  • order: Flavobacteriales
  • family: Flavobacteriaceae
  • genus: Flavobacterium
  • species: Flavobacterium alvei
  • full scientific name: Flavobacterium alvei Lee and Jeon 2018

@ref: 65583

domain: Bacteria

phylum: Bacteroidetes

class: Flavobacteriia

order: Flavobacteriales

family: Flavobacteriaceae

genus: Flavobacterium

species: Flavobacterium alvei

strain designation: HR-AY

type strain: yes

Morphology

cell morphology

  • @ref: 65583
  • gram stain: negative
  • cell length: 1.0-1.3 µm
  • cell width: 0.4-0.5 µm
  • cell shape: rod-shaped
  • motility: no

colony morphology

  • @ref: 65583
  • colony color: yellow
  • colony shape: circular
  • medium used: R2A

pigmentation

  • @ref: 65583
  • production: no
  • name: flexirubin-type

Culture and growth conditions

culture medium

  • @ref: 65583
  • name: Reasoner's 2A agar (R2A)
  • growth: yes

culture temp

@refgrowthtypetemperaturerange
65583positivegrowth5-30
65583positiveoptimum20psychrophilic
67770positivegrowth20psychrophilic

culture pH

@refabilitytypepHPH range
65583positivegrowth5.0-9.0alkaliphile
65583positiveoptimum7

Physiology and metabolism

oxygen tolerance

  • @ref: 65583
  • oxygen tolerance: obligate aerobe

halophily

  • @ref: 65583
  • salt: NaCl
  • growth: positive
  • tested relation: growth
  • concentration: 0 %

observation

  • @ref: 67770
  • observation: quinones: MK-6

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6558317128adipate-assimilation
6558327689decanoate-assimilation
65583casein-hydrolysis
6558317634D-glucose+assimilation
6558316899D-mannitol-assimilation
6558316024D-mannose+assimilation
655834853esculin+hydrolysis
655835291gelatin+hydrolysis
6558317234glucose+fermentation
6558330849L-arabinose+assimilation
6558325115malate-assimilation
6558317306maltose+assimilation
65583506227N-acetylglucosamine+assimilation
6558318401phenylacetate-assimilation
6558332032potassium gluconate-assimilation
6558353258sodium citrate-assimilation
6558328017starch-hydrolysis
6558353424tween 20-hydrolysis
6558353426tween 80-hydrolysis
6558318186tyrosine-hydrolysis

metabolite production

@refChebi-IDmetaboliteproduction
6558317997dinitrogenno
6558335581indoleno

enzymes

@refvalueactivityec
65583catalase-1.11.1.6
65583cytochrome oxidase-1.9.3.1
65583alkaline phosphatase+3.1.3.1
65583esterase (C 4)+
65583esterase Lipase (C 8)+
65583leucine arylamidase+3.4.11.1
65583acid phosphatase+3.1.3.2
65583naphthol-AS-BI-phosphohydrolase+
65583beta-galactosidase+3.2.1.23
65583beta-galactopyranosidase+
65583arginine dihydrolase+3.5.3.6
65583N-acetyl-beta-glucosaminidase+3.2.1.52
65583trypsin-3.4.21.4
65583alpha-galactosidase-3.2.1.22
65583alpha-fucosidase-3.2.1.51
65583lipase (C 14)-
65583alpha-chymotrypsin-3.4.21.1
65583alpha-mannosidase-3.2.1.24
65583urease-3.5.1.5
65583cystine arylamidase-3.4.11.3
65583beta-glucuronidase-3.2.1.31
65583alpha-glucosidase-3.2.1.20
65583valine arylamidase-

fatty acid profile

  • fatty acids

    @reffatty acidpercentage
    65583C12:01.5
    65583C16:05.8
    65583C13:0 iso1.8
    65583C14:0 iso1.4
    65583C15:0 iso13.3
    65583C13:0 anteiso1.6
    65583C15:0 anteiso15.3
    65583C15:0 2OH1
    65583C16:0 3OH4.5
    65583C17:0 2OH1.3
    65583C15:0 iso 3OH3.7
    65583C17:0 iso 3OH3.3
    65583C13:11.6
    65583C15:1 iso G1.2
    65583C15:1 anteiso A1.2
    65583C17:1 anteiso ω9c1.4
    65583C14:0 3OH / C16:1 iso I1.3
    65583C16:1ω7c / C16:1ω6c27.2
    65583C18:1ω7c / C18:1ω6c1.3
    6558310-methyl C16:0 / iso-C17:1ω9c1.1
  • type of FA analysis: whole cell analysis
  • incubation medium: R2A
  • incubation temperature: 20
  • software version: Sherlock 6.2B
  • library/peak naming table: TSBA6
  • system: MIS MIDI
  • method/protocol: Sasser, 1990
  • cutoff value:

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentlatitudelongitudeenrichment cultureenrichment culture durationenrichment culture temperatureisolation procedure
65583freshwater riverHan River, SeoulRepublic of KoreaKORAsia37.5094126.965R2A5 days25sample was serially diluted in phosphate-buffered saline (PBS) and 100 µl of each dilution was spread on R2A
67770Water sample of the Han RiverRepublic of KoreaKORAsia

isolation source categories

Cat1Cat2Cat3
#Environmental#Aquatic#Freshwater
#Environmental#Aquatic#River (Creek)

Sequence information

16S sequences

  • @ref: 65583
  • description: 16S rRNA gene sequence
  • accession: MF685248
  • database: nuccore

Genome sequences

  • @ref: 67770
  • description: Flavobacterium alvei HR-AY
  • accession: GCA_002920895
  • assembly level: contig
  • database: ncbi
  • NCBI tax ID: 2080416

GC content

  • @ref: 67770
  • GC-content: 34.4
  • method: genome sequence analysis

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
flagellatedno96.605no
gram-positiveno98.273yes
anaerobicno98.869yes
aerobicyes84.644yes
halophileno98.384yes
spore-formingno95.476no
glucose-utilyes90.288yes
thermophileno98.003no
motileno92.53yes
glucose-fermentno83.264yes

External links

@ref: 65583

culture collection no.: KACC 19407, JCM 32264

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny29676721Flavobacterium alvei sp. nov., isolated from a freshwater river.Lee Y, Jeon COInt J Syst Evol Microbiol10.1099/ijsem.0.0027682018Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Fatty Acids/chemistry, Flavobacterium/*classification/genetics/isolation & purification, Phosphatidylethanolamines/chemistry, *Phylogeny, Pigmentation, RNA, Ribosomal, 16S/genetics, Republic of Korea, Rivers/*microbiology, Sequence Analysis, DNA, Vitamin K 2/analogs & derivatives/chemistryTranscriptome
Phylogeny33108487Flavobacterium undicola sp. nov., isolated from a freshwater lake.Chen WM, Xie YR, Kwon SW, Sheu SYArch Microbiol10.1007/s00203-020-02096-32020Base Composition, DNA, Bacterial/genetics, Fatty Acids/chemistry, Flavobacterium/*classification/genetics, Lakes/*microbiology, Nucleic Acid Hybridization, Phospholipids/chemistry, *Phylogeny, Polyamines, RNA, Ribosomal, 16S/genetics, Species Specificity, Taiwan

Reference

@idauthorstitledoi/urljournalpubmed
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
65583Yunho Lee and Che Ok JeonFlavobacterium alvei sp. nov., isolated from a freshwater river10.1099/ijsem.0.002768IJSEM 68: 1919-1924 201829676721
66794Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar SchomburgBRENDA, the ELIXIR core data resource in 2021: new developments and updates10.1093/nar/gkaa1025Nucleic Acids Res. 49: D498-D508 202033211880
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/