Strain identifier
BacDive ID: 158804
Type strain:
Species: Winogradskyella pocilloporae
Strain Designation: AFPH31
Strain history: CIP <- 2017, S. Glaeser, Giessen Univ., Giessen, Germany: strain AFPH31
NCBI tax ID(s): 2161678 (species)
General
@ref: 65566
BacDive-ID: 158804
keywords: 16S sequence, Bacteria, obligate aerobe, halophilic, mesophilic, Gram-negative, rod-shaped, colony-forming
description: Winogradskyella pocilloporae AFPH31 is an obligate aerobe, halophilic, mesophilic bacterium that forms circular colonies and was isolated from internal healhy tissues of the scleractinian coral Pocillopora damicornis,which was cultured in an aquarium.
NCBI tax id
- NCBI tax id: 2161678
- Matching level: species
strain history
- @ref: 121919
- history: CIP <- 2017, S. Glaeser, Giessen Univ., Giessen, Germany: strain AFPH31
doi: 10.13145/bacdive158804.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/bacteroidota
- domain: Bacteria
- phylum: Bacteroidota
- class: Flavobacteriia
- order: Flavobacteriales
- family: Flavobacteriaceae
- genus: Winogradskyella
- species: Winogradskyella pocilloporae
- full scientific name: Winogradskyella pocilloporae Franco et al. 2018
@ref: 65566
domain: Bacteria
phylum: Bacteroidetes
class: Flavobacteriia
order: Flavobacteriales
family: Flavobacteriaceae
genus: Winogradskyella
species: Winogradskyella pocilloporae
strain designation: AFPH31
type strain: yes
Morphology
cell morphology
@ref | gram stain | cell length | cell width | cell shape | motility |
---|---|---|---|---|---|
65566 | negative | 1.8-2.2 µm | 0.7-1.1 µm | rod-shaped | no |
121919 | negative | rod-shaped | no |
colony morphology
- @ref: 65566
- colony color: shiny orange-yellow
- colony shape: circular
- incubation period: 3 days
- medium used: MA
pigmentation
@ref | production | name | color |
---|---|---|---|
65566 | no | flexirubin-type | |
65566 | yes | yellow |
Culture and growth conditions
culture medium
@ref | name | growth | composition | link |
---|---|---|---|---|
65566 | Reasoner's 2A agar (R2A) | yes | with 3 %(w/v) NaCl | |
65566 | Marine agar (MA) | yes | ||
65566 | Trypticase Soy Agar (TSA) | yes | with 3 %(w/v) NaCl | |
121919 | CIP Medium 13 | yes | https://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=13 |
culture temp
@ref | growth | type | temperature | range |
---|---|---|---|---|
65566 | positive | optimum | 25 | mesophilic |
65566 | positive | growth | 15-37 |
culture pH
@ref | ability | type | pH | PH range |
---|---|---|---|---|
65566 | positive | growth | 5.5-9.0 | alkaliphile |
65566 | positive | optimum | 6.0-8.5 |
Physiology and metabolism
oxygen tolerance
- @ref: 65566
- oxygen tolerance: obligate aerobe
halophily
@ref | halophily level | salt | growth | tested relation | concentration |
---|---|---|---|---|---|
65566 | halophilic | NaCl | positive | growth | 0.5-8.5 %(w/v) |
65566 | NaCl | positive | optimum | 1.0-4.5 %(w/v) |
metabolite utilization
@ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
---|---|---|---|---|
65566 | 30916 | 2-oxoglutarate | - | assimilation |
65566 | 16193 | 3-hydroxybenzoate | - | assimilation |
65566 | 37054 | 3-hydroxybutyrate | - | assimilation |
65566 | 16865 | gamma-aminobutyric acid | - | assimilation |
65566 | 17879 | 4-hydroxybenzoate | - | assimilation |
65566 | 16708 | adenine | - | hydrolysis |
65566 | 17128 | adipate | - | assimilation |
65566 | 15963 | ribitol | - | assimilation |
65566 | 15963 | ribitol | - | builds acid from |
65566 | 17925 | alpha-D-glucose | - | assimilation |
65566 | 17925 | alpha-D-glucose | + | builds acid from |
65566 | 27907 | alpha-L-rhamnose | - | assimilation |
65566 | 27907 | alpha-L-rhamnose | - | builds acid from |
65566 | 18305 | arbutin | - | assimilation |
65566 | 78208 | azelaate | - | assimilation |
65566 | 16958 | beta-alanine | - | assimilation |
65566 | 27689 | decanoate | - | assimilation |
65566 | casein | - | hydrolysis | |
65566 | 17057 | cellobiose | - | assimilation |
65566 | 17057 | cellobiose | - | builds acid from |
65566 | 62968 | cellulose | - | hydrolysis |
65566 | 16383 | cis-aconitate | - | assimilation |
65566 | 16947 | citrate | - | assimilation |
65566 | 18333 | D-arabitol | - | builds acid from |
65566 | 15824 | D-fructose | - | assimilation |
65566 | 12936 | D-galactose | - | assimilation |
65566 | 18391 | D-gluconate | - | assimilation |
65566 | 16899 | D-mannitol | - | builds acid from |
65566 | 16899 | D-mannitol | + | assimilation |
65566 | 16024 | D-mannose | - | assimilation |
65566 | 16024 | D-mannose | - | builds acid from |
65566 | 16988 | D-ribose | - | assimilation |
65566 | 17924 | D-sorbitol | - | assimilation |
65566 | 17924 | D-sorbitol | - | builds acid from |
65566 | 65327 | D-xylose | - | assimilation |
65566 | 65327 | D-xylose | + | builds acid from |
65566 | 24996 | lactate | - | assimilation |
65566 | 16991 | dna | - | hydrolysis |
65566 | 16813 | galactitol | - | builds acid from |
65566 | 17113 | erythritol | - | builds acid from |
65566 | 4853 | esculin | + | hydrolysis |
65566 | 29806 | fumarate | - | assimilation |
65566 | 5291 | gelatin | - | hydrolysis |
65566 | 17234 | glucose | - | assimilation |
65566 | 17234 | glucose | - | fermentation |
65566 | 17859 | glutaric acid | - | assimilation |
65566 | 17368 | hypoxanthine | + | hydrolysis |
65566 | 17268 | myo-inositol | - | builds acid from |
65566 | 17268 | myo-inositol | + | assimilation |
65566 | 17240 | itaconate | - | assimilation |
65566 | 16977 | L-alanine | - | assimilation |
65566 | 30849 | L-arabinose | - | assimilation |
65566 | 30849 | L-arabinose | + | builds acid from |
65566 | 29991 | L-aspartate | - | assimilation |
65566 | 29991 | L-aspartate | + | assimilation |
65566 | 15971 | L-histidine | - | assimilation |
65566 | 15603 | L-leucine | - | assimilation |
65566 | 15589 | L-malate | - | assimilation |
65566 | 15729 | L-ornithine | - | assimilation |
65566 | 17295 | L-phenylalanine | - | assimilation |
65566 | 17203 | L-proline | - | assimilation |
65566 | 17115 | L-serine | - | assimilation |
65566 | 16828 | L-tryptophan | + | assimilation |
65566 | 17716 | lactose | - | builds acid from |
65566 | 25115 | malate | - | assimilation |
65566 | 68428 | maltitol | - | assimilation |
65566 | 17306 | maltose | - | assimilation |
65566 | 17306 | maltose | + | builds acid from |
65566 | 28053 | melibiose | - | builds acid from |
65566 | 36986 | mesaconate | - | assimilation |
65566 | 320061 | methyl alpha-D-glucopyranoside | - | builds acid from |
65566 | 28037 | N-acetylgalactosamine | - | assimilation |
65566 | 506227 | N-acetylglucosamine | - | assimilation |
65566 | 17632 | nitrate | - | reduction |
65566 | 18401 | phenylacetate | - | assimilation |
65566 | 32032 | potassium gluconate | - | assimilation |
65566 | 17272 | propionate | - | assimilation |
65566 | 17148 | putrescine | - | assimilation |
65566 | 15361 | pyruvate | - | assimilation |
65566 | 16634 | raffinose | - | builds acid from |
65566 | 17814 | salicin | - | assimilation |
65566 | 17814 | salicin | - | builds acid from |
65566 | 32954 | sodium acetate | - | assimilation |
65566 | 53258 | sodium citrate | - | assimilation |
65566 | 28017 | starch | + | hydrolysis |
65566 | 9300 | suberic acid | - | assimilation |
65566 | 17992 | sucrose | - | assimilation |
65566 | 17992 | sucrose | - | builds acid from |
65566 | 15708 | trans-aconitate | - | assimilation |
65566 | 27082 | trehalose | - | assimilation |
65566 | 27082 | trehalose | - | builds acid from |
65566 | 53424 | tween 20 | - | hydrolysis |
65566 | 53423 | tween 40 | + | hydrolysis |
65566 | 53425 | tween 60 | + | hydrolysis |
65566 | 53426 | tween 80 | - | hydrolysis |
65566 | 18186 | tyrosine | - | hydrolysis |
65566 | 16199 | urea | - | hydrolysis |
65566 | 15318 | xanthine | + | hydrolysis |
65566 | 37166 | xylan | - | hydrolysis |
metabolite production
- @ref: 65566
- Chebi-ID: 35581
- metabolite: indole
- production: no
enzymes
@ref | value | activity | ec |
---|---|---|---|
65566 | catalase | + | 1.11.1.6 |
65566 | cytochrome oxidase | + | 1.9.3.1 |
65566 | alkaline phosphatase | + | 3.1.3.1 |
65566 | esterase (C 4) | + | |
65566 | esterase Lipase (C 8) | + | |
65566 | leucine arylamidase | + | 3.4.11.1 |
65566 | valine arylamidase | + | |
65566 | acid phosphatase | + | 3.1.3.2 |
65566 | naphthol-AS-BI-phosphohydrolase | + | |
65566 | lipase (C 14) | - | |
65566 | cystine arylamidase | - | 3.4.11.3 |
65566 | trypsin | - | 3.4.21.4 |
65566 | alpha-chymotrypsin | - | 3.4.21.1 |
65566 | alpha-galactosidase | - | 3.2.1.22 |
65566 | beta-glucuronidase | - | 3.2.1.31 |
65566 | alpha-glucosidase | - | 3.2.1.20 |
65566 | beta-glucosidase | - | 3.2.1.21 |
65566 | N-acetyl-beta-glucosaminidase | - | 3.2.1.52 |
65566 | alpha-mannosidase | - | 3.2.1.24 |
65566 | alpha-fucosidase | - | 3.2.1.51 |
65566 | arginine dihydrolase | - | 3.5.3.6 |
65566 | urease | - | 3.5.1.5 |
65566 | beta-galactosidase | - | 3.2.1.23 |
fatty acid profile
fatty acids
@ref fatty acid percentage 65566 C15:0 11.9 65566 C15:0 iso 22 65566 C15:1 iso G 16.9 65566 C16:0 iso 4.4 65566 C16:1 iso H 4.7 65566 C15:1ω6c 3.3 65566 C17:1ω6c 3.6 65566 C17:1 iso ω9c 3.2 65566 C15:0 iso 3OH 6 65566 C16:0 iso 3OH 4.4 65566 C17:0 iso 3OH 14.9 65566 unknown 13.565 1.6 65566 C16:1ω7c / C15:0 iso 2OH 3.1 - type of FA analysis: whole cell analysis
- incubation medium: MA
- incubation temperature: 25
- incubation time: 3
- library/peak naming table: TSBA4.1
- system: MIS MIDI
- method/protocol: Kämpfer & Kroppenstedt 1996
- cutoff value:
Isolation, sampling and environmental information
isolation
@ref | sample type | geographic location | country | origin.country | continent | enrichment culture | enrichment culture duration | enrichment culture temperature | isolation date |
---|---|---|---|---|---|---|---|---|---|
65566 | internal healhy tissues of the scleractinian coral Pocillopora damicornis,which was cultured in an aquarium | Justus Liebig University, Giessen | Germany | DEU | Europe | MA | 5 days | 28 | |
121919 | Environment, Tissue, coral Pocillopora damicornis | Giessen, Hessen | Germany | DEU | Europe | 2016-06-29 |
isolation source categories
Cat1 | Cat2 | Cat3 |
---|---|---|
#Host | #Invertebrates (Other) | #Cnidaria (Corals) |
#Engineered | #Built environment | #Water reservoir (Aquarium/pool) |
Safety information
risk assessment
- @ref: 121919
- biosafety level: 1
- biosafety level comment: Risk group (French classification)
Sequence information
16S sequences
- @ref: 65566
- description: 16S rRNA gene sequence
- accession: MG571571
- database: nuccore
GC content
- @ref: 65566
- GC-content: 36.8
- method: Thermal denaturation, fluorometry
External links
@ref: 65566
culture collection no.: CCM 8816, CIP 111546
straininfo link
- @ref: 111344
- straininfo: 412824
literature
- topic: Phylogeny
- Pubmed-ID: 29620493
- title: Winogradskyella pocilloporae sp. nov. isolated from healthy tissue of the coral Pocillopora damicornis.
- authors: Franco A, Busse HJ, Schubert P, Wilke T, Kampfer P, Glaeser SP
- journal: Int J Syst Evol Microbiol
- DOI: 10.1099/ijsem.0.002731
- year: 2018
- mesh: Animals, Anthozoa/*microbiology, Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Fatty Acids/chemistry, Flavobacteriaceae/*classification/genetics/isolation & purification, Germany, Phosphatidylethanolamines/chemistry, *Phylogeny, Pigmentation, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Spermidine/analogs & derivatives/chemistry, Vitamin K 2/analogs & derivatives/chemistry
- topic2: Transcriptome
Reference
@id | authors | title | doi/url | journal | pubmed | catalogue |
---|---|---|---|---|---|---|
20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | 10.1099/ijsem.0.004332 | |||
65566 | Angel Franco, Hans-Jürgen Busse, Patrick Schubert, Thomas Wilke, Peter Kämpfer and Stefanie P. Glaeser | Winogradskyella pocilloporae sp. nov. isolated from healthy tissue of the coral Pocillopora damicornis | 10.1099/ijsem.0.002731 | IJSEM 68: 1689-1696 2018 | 29620493 | |
111344 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | StrainInfo: A central database for resolving microbial strain identifiers | 10.60712/SI-ID412824.1 | |||
121919 | Curators of the CIP | https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20111546 | Collection of Institut Pasteur (CIP 111546) |