Strain identifier
version 8.1 (current version)
General
@ref: 65554
BacDive-ID: 158795
keywords: 16S sequence, Bacteria
description: Mycobacterium komaniense CIP 110823T is a bacterium that was isolated from bovine nasal membrane swab.
NCBI tax id
- NCBI tax id: 1566885
- Matching level: species
doi: 10.13145/bacdive158795.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/actinomycetota
- domain: Bacteria
- phylum: Actinomycetota
- class: Actinomycetes
- order: Mycobacteriales
- family: Mycobacteriaceae
- genus: Mycobacterium
- species: Mycobacterium komaniense
- full scientific name: Mycobacterium komaniense Gcebe et al. 2018
synonyms
- @ref: 20215
- synonym: Mycolicibacterium komanii
@ref: 65554
domain: Bacteria
phylum: Actinobacteria
class: Actinobacteria
order: Mycobacteriales
family: Mycobacteriaceae
genus: Mycobacterium
species: Mycobacterium komaniense
type strain: yes
Morphology
colony morphology
- @ref: 65554
- colony color: yellow
- medium used: LJ slants
Culture and growth conditions
culture medium
| @ref | name | growth | composition |
|---|---|---|---|
| 65554 | LJ slants | yes | supplemented with PACT |
| 65554 | Middlebrook 7H11 | yes | supplemented with 0.1% OADC |
culture temp
| @ref | growth | type | temperature | range |
|---|---|---|---|---|
| 65554 | positive | growth | 25-45 | |
| 65554 | positive | optimum | 37 | mesophilic |
Physiology and metabolism
halophily
- @ref: 65554
- salt: NaCl
- growth: no
- tested relation: growth
- concentration: 5 %
observation
- @ref: 65554
- observation: acid-fast
metabolite utilization
| @ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
|---|---|---|---|---|
| 65554 | 16947 | citrate | - | hydrolysis |
| 65554 | 16899 | D-mannitol | - | carbon source |
| 65554 | 4853 | esculin | - | hydrolysis |
| 65554 | 17268 | myo-inositol | - | carbon source |
| 65554 | 30849 | L-arabinose | - | carbon source |
| 65554 | 62345 | L-rhamnose | - | carbon source |
| 65554 | 53426 | tween 80 | + | hydrolysis |
antibiotic resistance
| @ref | ChEBI | metabolite | is antibiotic | is sensitive | sensitivity conc. | is resistant | resistance conc. |
|---|---|---|---|---|---|---|---|
| 65554 | 100241 | ciprofloxacin | yes | yes | 10 µg (disc) | ||
| 65554 | 3732 | clarithromycin | yes | yes | 15 µg (disc) | ||
| 65554 | 2637 | amikacin | yes | yes | 30 µg (disc) | ||
| 65554 | 50845 | doxycycline | yes | yes | 30 µg (disc) | ||
| 65554 | 471744 | imipenem | yes | yes | 10 µg (disc) | ||
| 65554 | 28864 | tobramycin | yes | yes | 10 µg (disc) | ||
| 65554 | 209807 | cefoxitin | yes | yes | 10 µg (disc) | ||
| 65554 | 2676 | amoxicillin | yes | yes | 30 µg (disc) |
enzymes
| @ref | value | activity | ec |
|---|---|---|---|
| 65554 | nitrate reductase | + | 1.7.99.4 |
| 65554 | urease | + | 3.5.1.5 |
| 65554 | pyrazinamidase | + | 3.5.1.B15 |
| 65554 | arylsulfatase | +/- | 3.1.6.1 |
| 65554 | catalase | +/- | 1.11.1.6 |
Isolation, sampling and environmental information
isolation
- @ref: 65554
- sample type: bovine nasal membrane swab
- sampling date: 2010 - 2012
- geographic location: Queenstown, Eastern Cape Province
- country: South Africa
- origin.country: ZAF
- continent: Africa
isolation source categories
| Cat1 | Cat2 | Cat3 |
|---|---|---|
| #Host | #Mammals | #Bovinae (Cow, Cattle) |
| #Host Body-Site | #Organ | #Nose |
| #Infection | #Patient | #Swab |
Sequence information
16S sequences
- @ref: 65554
- description: 16S rRNA gene sequence
- accession: KJ873240
- database: nuccore
GC content
- @ref: 65554
- GC-content: 67.33
- method: genome sequence analysis
External links
@ref: 65554
culture collection no.: CIP 110823T, ATCC BAA 2758, GPK 1020
literature
- topic: Phylogeny
- Pubmed-ID: 29543151
- title: Mycobacterium komaniense sp. nov., a rapidly growing non-tuberculous Mycobacterium species detected in South Africa.
- authors: Gcebe N, Rutten VPMG, van Pittius NG, Naicker B, Michel AL
- journal: Int J Syst Evol Microbiol
- DOI: 10.1099/ijsem.0.002707
- year: 2018
- mesh: Animals, Bacterial Typing Techniques, Cattle/*microbiology, DNA, Bacterial/genetics, Genes, Bacterial, Nontuberculous Mycobacteria/*classification/genetics/isolation & purification, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, South Africa
- topic2: Transcriptome
Reference
| @id | authors | title | doi/url | journal | pubmed |
|---|---|---|---|---|---|
| 20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | 10.1099/ijsem.0.004332 | ||
| 65554 | Nomakorinte Gcebe, Victor P. M. G. Rutten, Nicolaas Gey van Pittius, Brendon Naicker and Anita L. Michel | Mycobacterium komaniense sp. nov., a rapidly growing non-tuberculous Mycobacterium species detected in South Africa | 10.1099/ijsem.0.002707 | IJSEM 68: 1526-1532 2018 | 29543151 |