Strain identifier

BacDive ID: 158753

Type strain: Yes

Species: Paracoccus sordidisoli

Strain Designation: LP91

NCBI tax ID(s): 1798717 (species)

« back to strain detail view
For citation purpose refer to the digital object identifier (doi) of the current version.
Archive
version 8.1 (current version):
version 8:
version 7.1:
version 7:
version 6:
version 5:
version 4.1:
version 8.1 (current version)

General

@ref: 65505

BacDive-ID: 158753

DSM-Number: 101680

keywords: 16S sequence, Bacteria, aerobe, Gram-negative, rod-shaped, colony-forming

description: Paracoccus sordidisoli LP91 is an aerobe, Gram-negative, rod-shaped bacterium that forms circular colonies and was isolated from from an agricultural field contaminated with hexachlorocyclohexane .

NCBI tax id

  • NCBI tax id: 1798717
  • Matching level: species

doi: 10.13145/bacdive158753.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Alphaproteobacteria
  • order: Rhodobacterales
  • family: Paracoccaceae
  • genus: Paracoccus
  • species: Paracoccus sordidisoli
  • full scientific name: Paracoccus sordidisoli Singh et al. 2017

@ref: 65505

domain: Bacteria

phylum: Proteobacteria

class: Alphaproteobacteria

order: Rhodobacterales

family: Rhodobacteraceae

genus: Paracoccus

species: Paracoccus sordidisoli

strain designation: LP91

type strain: yes

Morphology

cell morphology

  • @ref: 65505
  • gram stain: negative
  • cell length: 0.8-1.3 µm
  • cell width: 0.4-0.7 µm
  • cell shape: rod-shaped

colony morphology

  • @ref: 65505
  • colony color: creamy white
  • colony shape: circular

pigmentation

  • @ref: 65505
  • production: no
  • name: flexirubin-type

Culture and growth conditions

culture medium

@refnamegrowth
65505Marine agar (MA)yes
65505BHIyes
65505LB (Luria-Bertani) MEDIUMyes
65505Reasoner's 2A agar (R2A)yes
65505Nutrient agar (NA)yes
65505TSYEAyes

culture temp

@refgrowthtypetemperaturerange
65505positivegrowth8-40
65505positiveoptimum28mesophilic

culture pH

@refabilitytypepHPH range
65505positivegrowth5-11alkaliphile
65505positiveoptimum6-9

Physiology and metabolism

oxygen tolerance

  • @ref: 65505
  • oxygen tolerance: aerobe

halophily

@refsaltgrowthtested relationconcentration
65505NaClpositivegrowth0-6 %
65505NaClpositiveoptimum0-4 %

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
65505168082-dehydro-D-gluconate-builds acid from
65505581435-dehydro-D-gluconate-builds acid from
6550517128adipate+fermentation
6550527613amygdalin-builds acid from
6550518305arbutin-builds acid from
6550527689decanoate-assimilation
65505casein-hydrolysis
6550517057cellobiose-builds acid from
6550515963ribitol-builds acid from
6550518333D-arabitol-builds acid from
6550515824D-fructose+builds acid from
6550528847D-fucose+builds acid from
6550512936D-galactose+builds acid from
6550517634D-glucose+assimilation
6550517634D-glucose+builds acid from
6550517634D-glucose+fermentation
6550562318D-lyxose+builds acid from
6550516899D-mannitol-assimilation
6550516899D-mannitol-builds acid from
6550516024D-mannose+builds acid from
6550516024D-mannose+fermentation
6550516988D-ribose+builds acid from
6550517924D-sorbitol-builds acid from
6550516443D-tagatose-builds acid from
6550565327D-xylose+builds acid from
6550516991dna+hydrolysis
6550516813galactitol-builds acid from
6550517113erythritol-builds acid from
65505esculin ferric citrate+assimilation
65505esculin ferric citrate+builds acid from
6550528066gentiobiose-builds acid from
6550517754glycerol-builds acid from
6550528087glycogen-builds acid from
6550517268myo-inositol+builds acid from
6550515443inulin-builds acid from
6550530849L-arabinose+builds acid from
6550530849L-arabinose+fermentation
6550518403L-arabitol-builds acid from
6550518287L-fucose+builds acid from
6550562345L-rhamnose+builds acid from
6550517266L-sorbose-builds acid from
6550565328L-xylose+builds acid from
6550517716lactose-builds acid from
6550525115malate+fermentation
6550517306maltose-assimilation
6550517306maltose-builds acid from
655056731melezitose-builds acid from
6550528053melibiose-builds acid from
65505320061methyl alpha-D-glucopyranoside-builds acid from
6550543943methyl alpha-D-mannoside-builds acid from
6550574863methyl beta-D-xylopyranoside-builds acid from
65505506227N-acetylglucosamine-builds acid from
65505506227N-acetylglucosamine+fermentation
6550517632nitrate-reduction
6550518401phenylacetate-assimilation
6550532032potassium gluconate-assimilation
6550532032potassium gluconate-builds acid from
6550516634raffinose-builds acid from
6550517814salicin-builds acid from
6550553258sodium citrate+fermentation
6550517992sucrose-builds acid from
6550527082trehalose-builds acid from
6550532528turanose-builds acid from
6550553424tween 20+hydrolysis
6550553426tween 80+hydrolysis
6550517151xylitol-builds acid from

antibiotic resistance

@refChEBImetaboliteis antibioticis sensitivesensitivity conc.is resistantresistance conc.
6550528077rifampicinyesyes5 µg (disc)
65505100241ciprofloxacinyesyes5 µg (disc)
6550517076streptomycinyesyes10 µg (disc)
6550518208penicillin gyesyes10 µg (disc)
6550517833gentamicinyesyes10 µg (disc)
6550517698chloramphenicolyesyes30 µg (disc)
6550528001vancomycinyesyes30 µg (disc)
655056104kanamycinyesyes30 µg (disc)
6550527701oxytetracyclineyesyes30 µg (disc)
655052637amikacinyesyes30 µg (disc)
6550527902tetracyclineyesyes30 µg (disc)
655058309polymyxin byesyes300 µg (disc)
6550528971ampicillinyesyes10 µg (disc)
65505100147nalidixic acidyesyes30 µg (disc)

enzymes

@refvalueactivityec
65505catalase+1.11.1.6
65505cytochrome oxidase+1.9.3.1
65505arginine dihydrolase-3.5.3.6
65505gelatinase-
65505beta-galactosidase-3.2.1.23
65505urease+3.5.1.5

fatty acid profile

  • fatty acids

    @reffatty acidpercentage
    65505C9:00.3
    65505C14:00.5
    65505C15:0 anteiso0.2
    65505C16:04.2
    65505C17:00.8
    65505C18:01.1
    65505C18:0 iso2.4
    65505C10:0 3OH2.6
    65505C12:1 3OH0.2
    65505C15:0 3OH0.1
    65505C17:1ω8c0.3
    65505C17:1ω6c0.2
    65505C18:1ω5c0.2
    65505C20:4ω6,9,12,15c0.2
    65505C20:1ω7c0.5
    65505C14:0 3-OH / iso-C16:1 I / C12:0 aldehyde1.9
    65505C16:1ω7c / C16:1ω6c2.3
    65505C18:1ω7c / C18:1ω6c81.7
  • type of FA analysis: whole cell analysis
  • incubation medium: LB
  • incubation temperature: 28
  • incubation time: 2
  • library/peak naming table: RTSBA6
  • system: MIS MIDI

Isolation, sampling and environmental information

isolation

  • @ref: 65505
  • sample type: from an agricultural field contaminated with hexachlorocyclohexane (HCH)
  • sampling date: 2014-05-14
  • geographic location: Ummari village, Lucknow, Uttar Pradesh
  • country: India
  • origin.country: IND
  • continent: Asia
  • latitude: 27.0349
  • longitude: 81.1384
  • enrichment culture: mineral salt medium
  • enrichment culture composition: (NH4)2HPO4 0.5 g/l, MgSO4 . 7H2O 0.2 g/l, FeSO4 . 7H2O 0.01 g/l, K2HPO4 0.1 g/l, Ca(NO3)2 0.01 g/l and supplemented with 0.3 % glucose (pH 7.5)
  • enrichment culture temperature: 28
  • isolation procedure: serial dilutions were spread on Luria-Bertani (LB) agar plates containing streptomycin (10 µg/ml) and nystatin (20 µg/ml)

isolation source categories

Cat1Cat2Cat3
#Engineered#Agriculture#Field
#Engineered#Contamination#Pesticide

Sequence information

16S sequences

  • @ref: 65505
  • description: 16S rRNA gene sequence
  • accession: KU693337.2
  • database: nuccore

GC content

  • @ref: 65505
  • GC-content: 67.2
  • method: real time PCR (RTD-PCR)

External links

@ref: 65505

culture collection no.: KCTC 42938, CCM 8696, MCC 3128, DSM 101680

literature

  • topic: Phylogeny
  • Pubmed-ID: 28933319
  • title: Paracoccus sordidisoli sp. nov., isolated from an agricultural field contaminated with hexachlorocyclohexane isomers.
  • authors: Singh AK, Kohli P, Mahato NK, Lal R
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijsem.0.002289
  • year: 2017
  • mesh: Agriculture, Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Fatty Acids/chemistry, Hexachlorocyclohexane/analysis, India, Nucleic Acid Hybridization, Paracoccus/*classification/genetics/isolation & purification, Phospholipids/chemistry, *Phylogeny, Polyamines/chemistry, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, *Soil Microbiology, Soil Pollutants/*analysis, Ubiquinone/chemistry
  • topic2: Transcriptome

Reference

@idauthorstitledoi/urljournalpubmed
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
65505Amit Kumar Singh, Puneet Kohli, Nitish Kumar Mahato and Rup LalParacoccus sordidisoli sp. nov., isolated from an agricultural field contaminated with hexachlorocyclohexane isomers10.1099/ijsem.0.002289IJSEM 67: 4365-4371 201728933319