Strain identifier

BacDive ID: 158737

Type strain: Yes

Species: Roseovarius ramblicola

Strain Designation: D15

NCBI tax ID(s): 2022336 (species)

« back to strain detail view
For citation purpose refer to the digital object identifier (doi) of the current version.
Archive
version 8.1 (current version):
version 8:
version 7.1:
version 7:
version 6:
version 5:
version 4.1:
version 8.1 (current version)

General

@ref: 65488

BacDive-ID: 158737

keywords: 16S sequence, Bacteria, rod-shaped, colony-forming

description: Roseovarius ramblicola D15 is a rod-shaped bacterium that forms circular colonies and was isolated from a sample of soil taken from Rambla Salada , a hypersaline rambla .

NCBI tax id

  • NCBI tax id: 2022336
  • Matching level: species

doi: 10.13145/bacdive158737.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Alphaproteobacteria
  • order: Rhodobacterales
  • family: Roseobacteraceae
  • genus: Roseovarius
  • species: Roseovarius ramblicola
  • full scientific name: Roseovarius ramblicola Castro et al. 2018

@ref: 65488

domain: Bacteria

phylum: Proteobacteria

class: Alphaproteobacteria

order: Rhodobacterales

family: Rhodobacteraceae

genus: Roseovarius

species: Roseovarius ramblicola

strain designation: D15

type strain: yes

Morphology

cell morphology

@refcell shapegram staincell lengthcell widthmotility
65488rod-shaped
65488ovoid-shapednegative2.1-3.2 µm0.7-1.2 µmno

colony morphology

  • @ref: 65488
  • colony shape: circular
  • medium used: marine agar, Reasoner's 2A agar

Culture and growth conditions

culture medium

  • @ref: 65488
  • name: marine agar, Reasoner's 2A agar
  • growth: yes

culture temp

@refgrowthtypetemperaturerange
65488positivegrowth5-30
65488positiveoptimum28mesophilic

culture pH

@refabilitytypepHPH range
65488positivegrowth6-9alkaliphile
65488positiveoptimum7

Physiology and metabolism

halophily

@refsaltgrowthtested relationconcentration
65488NaClpositivegrowth0.5-10 %(w/v)
65488NaClpositiveoptimum3 %(w/v)

observation

@refobservation
65488reproduce by budding or asymmetrical division
65488growth pattern is uniform in a liquid medium
65488only detected lipoquinone is ubiquinone with ten isoprene units (Q10)
65488polar lipids contain phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol, aminolipid and three unidentified polar lipids

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6548817128adipate-assimilation
6548827613amygdalin-builds acid from
6548818305arbutin-builds acid from
6548829016arginine-hydrolysis
6548827689decanoate-assimilation
6548817057cellobiose-builds acid from
6548815963ribitol-builds acid from
6548817108D-arabinose-builds acid from
6548818333D-arabitol-builds acid from
6548815824D-fructose-builds acid from
6548828847D-fucose-builds acid from
6548812936D-galactose-builds acid from
6548817634D-glucose-assimilation
6548817634D-glucose-builds acid from
6548862318D-lyxose-builds acid from
6548816899D-mannitol-builds acid from
6548816024D-mannose-builds acid from
6548816988D-ribose-builds acid from
6548817924D-sorbitol-builds acid from
6548816443D-tagatose-builds acid from
6548865327D-xylose-builds acid from
6548816813galactitol-builds acid from
6548817113erythritol-builds acid from
654884853esculin-hydrolysis
65488esculin ferric citrate-builds acid from
654885291gelatin-hydrolysis
6548828066gentiobiose-builds acid from
6548824265gluconate-assimilation
6548817754glycerol-builds acid from
6548828087glycogen-builds acid from
6548817268myo-inositol-builds acid from
6548815443inulin-builds acid from
6548830849L-arabinose-assimilation
6548830849L-arabinose-builds acid from
6548818403L-arabitol-builds acid from
6548818287L-fucose-builds acid from
6548862345L-rhamnose-builds acid from
6548817266L-sorbose-builds acid from
6548865328L-xylose-builds acid from
6548817716lactose-builds acid from
6548825115malate-assimilation
6548817306maltose-assimilation
6548817306maltose-builds acid from
6548837684mannose-assimilation
654886731melezitose-builds acid from
6548828053melibiose-builds acid from
65488320061methyl alpha-D-glucopyranoside-builds acid from
6548843943methyl alpha-D-mannoside-builds acid from
6548874863methyl beta-D-xylopyranoside-builds acid from
65488506227N-acetylglucosamine-assimilation
65488506227N-acetylglucosamine-builds acid from
6548817632nitrate-reduction
6548816301nitrite-reduction
6548818401phenylacetate-assimilation
65488potassium 2-dehydro-D-gluconate-builds acid from
65488potassium 5-dehydro-D-gluconate-builds acid from
6548832032potassium gluconate-builds acid from
6548816634raffinose-builds acid from
6548817814salicin-builds acid from
6548853258sodium citrate-assimilation
6548828017starch-builds acid from
6548817992sucrose-builds acid from
6548827082trehalose-builds acid from
6548832528turanose-builds acid from
6548817151xylitol-builds acid from

antibiotic resistance

@refChEBImetaboliteis antibioticis sensitivegroup IDis resistant
654882676amoxicillinyesyes
654882676amoxicillinyesyes11
6548848947clavulanic acidyesyes11
6548828971ampicillinyesyes
654882955azithromycinyesyes
65488161680aztreonamyesyes
6548828669bacitracinyesyes
654883393carbenicillinyesyes
65488204928cefotaximeyesyes
65488124991cefalotinyesyes
654883515cefuroximeyesyes
6548817698chloramphenicolyesyes
6548848923erythromycinyesyes
6548828077rifampicinyesyes
6548817334penicillinyesyes
6548828864tobramycinyesyes
6548845924trimethoprimyesyes26
654889332sulfamethoxazoleyesyes26
65488100246norfloxacinyesyes
6548835358sulfonamideyesyes
6548817076streptomycinyesyes
6548817833gentamicinyesyes
65488100147nalidixic acidyesyes
654888309polymyxin byesyes
65488100241ciprofloxacinyesyes
6548827902tetracyclineyesyes
654882637amikacinyesyes
654887507neomycinyesyes

metabolite production

  • @ref: 65488
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

enzymes

@refvalueactivityec
65488catalase+1.11.1.6
65488cytochrome oxidase+1.9.3.1
65488urease-3.5.1.5
65488leucine arylamidase+3.4.11.1
65488esterase (C 4)-
65488esterase Lipase (C 8)-
65488naphthol-AS-BI-phosphohydrolase-
65488alkaline phosphatase-3.1.3.1
65488lipase (C 14)-
65488valine arylamidase-
65488cystine arylamidase-3.4.11.3
65488trypsin-3.4.21.4
65488alpha-chymotrypsin-3.4.21.1
65488alpha-galactosidase-3.2.1.22
65488beta-galactosidase-3.2.1.23
65488beta-glucuronidase-3.2.1.31
65488alpha-glucosidase-3.2.1.20
65488beta-glucosidase-3.2.1.21
65488N-acetyl-beta-glucosaminidase-3.2.1.52
65488alpha-mannosidase-3.2.1.24
65488alpha-fucosidase-3.2.1.51

fatty acid profile

  • fatty acids

    @reffatty acidpercentage
    65488C12:0 2OH0.9
    65488C12:1 3OH3.6
    65488C12:07.1
    65488C16:011.9
    65488C18:01.8
    65488C16:0 2OH2.5
    65488C18:1ω9c0.7
    65488C18:1ω7c 11-methyl2.3
    65488Cyclo-C19:0ω8c0.9
    65488C16:1ω7c/C16:1ω6c0.8
    65488C18:1ω7c61.5
  • type of FA analysis: whole cell analysis
  • incubation medium: marine agar
  • incubation temperature: 30
  • incubation time: 2
  • library/peak naming table: TSBA6 145
  • system: MIS MIDI
  • instrument: Agilent 6850 gas chromatograph
  • method/protocol: Sasser, 1990

Isolation, sampling and environmental information

isolation

  • @ref: 65488
  • sample type: a sample of soil taken from Rambla Salada (Murcia), a hypersaline rambla (a steep-sided river bed, normally dry but subject to flash flooding)
  • country: Spain
  • origin.country: ESP
  • continent: Europe
  • latitude: 38.1241
  • longitude: -1.117
  • enrichment culture composition: S3, a lownutrient medium supplemented with 3 % (w/v) sea-salt solution
  • enrichment culture duration: 30 days
  • enrichment culture temperature: 25
  • isolation procedure: serial dilutions from 1g soil were prepared,previously sonicated for 30s, in 10ml supplemented S3 medium. The number of micro-organisms in each dilution was determined using a Petroff Hausser counting chamber using methylene blue as contrast. A 48-well microtiter plate, containing 490µl supplemented S3 medium, was inoculated with 10µl dilution containing 100 bacteria per millilitre (approximately 1 bacterium per well).

isolation source categories

Cat1Cat2Cat3
#Environmental#Aquatic#River (Creek)
#Environmental#Terrestrial#Soil
#Condition#Saline

Sequence information

16S sequences

  • @ref: 65488
  • description: 16S rRNA gene sequence
  • accession: MF527111
  • database: nuccore

GC content

  • @ref: 65488
  • GC-content: 63
  • method: thermal denaturation, midpoint method (Tm)

External links

@ref: 65488

culture collection no.: CECT 9424, LMG 30322

literature

  • topic: Phylogeny
  • Pubmed-ID: 29664362
  • title: Roseovarius ramblicola sp. nov., a moderately halophilic bacterium isolated from saline soil in Spain.
  • authors: Castro DJ, Cerezo I, Sampedro I, Martinez-Checa F
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijsem.0.002744
  • year: 2018
  • mesh: Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Fatty Acids/chemistry, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Rhodobacteraceae/*classification/genetics/isolation & purification, *Salinity, Sequence Analysis, DNA, Soil, *Soil Microbiology, Spain, Ubiquinone/chemistry
  • topic2: Transcriptome

Reference

@idauthorstitledoi/urljournalpubmed
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
65488David J. Castro, Isabel Cerezo, Inmaculada Sampedro, Fernando Martínez-ChecaRoseovarius ramblicola sp. nov., a moderately halophilic bacterium isolated from saline soil in Spain10.1099/ijsem.0.002744IJSEM 68: 1839-1844 201829664362