Strain identifier

BacDive ID: 158718

Type strain: Yes

Species: Neptunicella marina

Strain Designation: S27-2

NCBI tax ID(s): 2125989 (species)

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General

@ref: 65462

BacDive-ID: 158718

keywords: genome sequence, 16S sequence, Bacteria, aerobe, motile, rod-shaped, colony-forming

description: Neptunicella marina S27-2 is an aerobe, motile, rod-shaped bacterium that forms circular colonies and was isolated from a surface seawater sample during cruise DY105-17A of the R/V Da-Yang Yi-Hao, at site IR-CTD13.

NCBI tax id

  • NCBI tax id: 2125989
  • Matching level: species

doi: 10.13145/bacdive158718.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Alteromonadales
  • family: Alteromonadaceae
  • genus: Neptunicella
  • species: Neptunicella marina
  • full scientific name: Neptunicella marina Liu et al. 2018

@ref: 65462

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Alteromonadales

family: Alteromonadaceae

genus: Neptunicella

species: Neptunicella marina

strain designation: S27-2

type strain: yes

Morphology

cell morphology

@refcell lengthcell widthcell shapemotilityflagellum arrangementgram stain
654621.0-2.0 µm0.5-1 µmrod-shapedyespolar
65462negative

colony morphology

  • @ref: 65462
  • colony color: creamy white
  • colony shape: circular
  • medium used: marine agar

Culture and growth conditions

culture temp

@refgrowthtypetemperaturerange
65462positivegrowth10-41
65462positiveoptimum25-37mesophilic

culture pH

@refabilitytypepHPH range
65462positivegrowth6-9alkaliphile
65462positiveoptimum7

Physiology and metabolism

oxygen tolerance

  • @ref: 65462
  • oxygen tolerance: aerobe

halophily

@refsaltgrowthtested relationconcentration
65462NaClpositivegrowth0-12 %
65462NaClpositiveminimum0.5-2 %

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6546217128adipate+assimilation
6546227613amygdalin+fermentation
6546222599arabinose-fermentation
6546227689decanoate+assimilation
65462casein-
6546217634D-glucose+assimilation
6546217634D-glucose+fermentation
6546216899D-mannitol+assimilation
6546216024D-mannose+assimilation
6546230849L-arabinose+assimilation
6546225115malate+assimilation
6546217306maltose+assimilation
6546229864mannitol-fermentation
6546228053melibiose-fermentation
6546217268myo-inositol-fermentation
65462506227N-acetylglucosamine+assimilation
6546217632nitrate-reduction
6546218401phenylacetate+assimilation
6546232032potassium gluconate+assimilation
6546226546rhamnose-fermentation
6546253258sodium citrate+assimilation
6546230911sorbitol-fermentation
6546228017starch-
6546217992sucrose+fermentation
6546253424tween 20+hydrolysis
6546253423tween 40+hydrolysis
6546253425tween 60+hydrolysis
6546253426tween 80+hydrolysis

metabolite production

@refChebi-IDmetaboliteproduction
6546215688acetoinyes
6546235581indoleno
6546215138sulfideno

metabolite tests

  • @ref: 65462
  • Chebi-ID: 15688
  • metabolite: acetoin
  • voges-proskauer-test: +

enzymes

@refvalueactivityec
65462alkaline phosphatase+3.1.3.1
65462esterase (C 4)+
65462esterase Lipase (C 8)+
65462leucine arylamidase+3.4.11.1
65462valine arylamidase+
65462cystine arylamidase+3.4.11.3
65462acid phosphatase+3.1.3.2
65462naphthol-AS-BI-phosphohydrolase+
65462alpha-glucosidase+3.2.1.20
65462N-acetyl-beta-glucosaminidase+3.2.1.52
65462lipase (C 14)+
65462alpha-chymotrypsin+3.4.21.1
65462alpha-galactosidase+3.2.1.22
65462alpha-mannosidase+3.2.1.24
65462trypsin-3.4.21.4
65462beta-galactosidase-3.2.1.23
65462beta-glucuronidase-3.2.1.31
65462alpha-fucosidase-3.2.1.51
65462beta-glucosidase+3.2.1.21
65462gelatinase+
65462arginine dihydrolase-3.5.3.6
65462lysine decarboxylase-4.1.1.18
65462ornithine decarboxylase-4.1.1.17
65462urease-3.5.1.5

Isolation, sampling and environmental information

isolation

  • @ref: 65462
  • sample type: a surface seawater sample during cruise DY105-17A of the R/V Da-Yang Yi-Hao, at site IR-CTD13
  • sampling date: December 2005
  • geographic location: Indian Ocean
  • latitude: -24.2666
  • longitude: 69.7833
  • enrichment culture: PTA marine agar medium
  • enrichment culture composition: per litre of sea water; 2.0 g sodium acetate, 0.5 g tryptone, 0.5 g yeast extract, 0.5 g potato extract, 0.2 g glucose, 0.2 g sucrose, 2.0 g NH 4 NO 3; pH 7.4-7.5
  • isolation procedure: Crude oil was used as a carbon and energy source to enrich oil-degrading bacteria. Strain S27-2 was isolated by using a plate-screening method and purified by restreaking it onto plates of PTA marine agar medium

isolation source categories

Cat1Cat2Cat3
#Environmental#Aquatic#Marine
#Environmental#Aquatic#Surface water

Sequence information

16S sequences

  • @ref: 65462
  • description: 16S rRNA gene sequence
  • accession: KX977388
  • database: nuccore

Genome sequences

  • @ref: 66792
  • description: Neptunicella marina S27-2
  • accession: GCA_014270035
  • assembly level: contig
  • database: ncbi
  • NCBI tax ID: 2125989

GC content

  • @ref: 65462
  • GC-content: 43.7
  • method: high performance liquid chromatography (HPLC)

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
flagellatedyes79.37no
gram-positiveno99.175no
anaerobicno98.13yes
aerobicyes90.074yes
halophileno54.777no
spore-formingno96.876no
thermophileno99.409no
glucose-utilyes95.65yes
motileyes92.514yes
glucose-fermentno73.759no

External links

@ref: 65462

culture collection no.: KCTC 52335, MCCC 1A02149

literature

  • topic: Phylogeny
  • Pubmed-ID: 29533174
  • title: Neptunicella marina gen. nov., sp. nov., isolated from surface seawater.
  • authors: Liu X, Lai Q, Du Y, Zhang X, Liu Z, Sun F, Shao Z
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijsem.0.002660
  • year: 2018
  • mesh: Alteromonadaceae/*classification/genetics/isolation & purification, Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Fatty Acids/chemistry, Indian Ocean, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Seawater/*microbiology, Sequence Analysis, DNA, Ubiquinone/chemistry, *Water Microbiology
  • topic2: Transcriptome

Reference

@idauthorstitledoi/urljournalpubmed
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
65462Xiupian Liu, Qiliang Lai, Yaping Du, Xiaorong Zhang, Zhen Liu, Fengqin Sun, Zongze ShaoNeptunicella marina gen. nov., sp. nov., isolated from surface seawater10.1099/ijsem.0.002660IJSEM 68: 1423-1428 201829533174
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes