Strain identifier

BacDive ID: 158715

Type strain: Yes

Species: Pseudomaribius aestuariivivens

Strain Designation: DBTF-15

NCBI tax ID(s): 2050951 (species)

For citation purpose refer to the digital object identifier (doi) of the current version.
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General

@ref: 65457

BacDive-ID: 158715

keywords: 16S sequence, Bacteria, obligate aerobe, mesophilic, Gram-negative, rod-shaped, colony-forming

description: Pseudomaribius aestuariivivens DBTF-15 is an obligate aerobe, mesophilic, Gram-negative bacterium that forms circular colonies and was isolated from a tidal flat sediment collected from Daebu island on the Yellow Sea.

NCBI tax id

  • NCBI tax id: 2050951
  • Matching level: species

doi: 10.13145/bacdive158715.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Alphaproteobacteria
  • order: Rhodobacterales
  • family: Roseobacteraceae
  • genus: Pseudomaribius
  • species: Pseudomaribius aestuariivivens
  • full scientific name: Pseudomaribius aestuariivivens Park et al. 2018

@ref: 65457

domain: Bacteria

phylum: Proteobacteria

class: Alphaproteobacteria

order: Rhodobacterales

family: Rhodobacteraceae

genus: Pseudomaribius

species: Pseudomaribius aestuariivivens

strain designation: DBTF-15

type strain: yes

Morphology

cell morphology

  • @ref: 65457
  • gram stain: negative
  • cell length: 0.3-1.6 µm
  • cell width: 0.5-1.0 µm
  • cell shape: rod-shaped
  • motility: no

colony morphology

  • @ref: 65457
  • colony size: 0.5-0.8 mm
  • colony color: greyish yellow
  • colony shape: circular
  • incubation period: 7 days
  • medium used: marine agar

Culture and growth conditions

culture medium

  • @ref: 65457
  • name: Marine agar (MA)
  • growth: yes

culture temp

@refgrowthtypetemperaturerange
65457positivegrowth30mesophilic
65457positiveoptimum25-30mesophilic
65457positivegrowth10psychrophilic
65457positivegrowth37mesophilic
65457nogrowth40thermophilic
65457nogrowth4psychrophilic

culture pH

@refabilitytypepH
65457positiveoptimum7-8
65457positivegrowth6
65457nogrowth5.5

Physiology and metabolism

oxygen tolerance

  • @ref: 65457
  • oxygen tolerance: obligate aerobe

spore formation

  • @ref: 65457
  • spore formation: no

halophily

@refsaltgrowthtested relationconcentration
65457NaClpositivegrowth0.5-5.0 %(w/v)
65457NaClpositiveoptimum2 %(w/v)

observation

@refobservation
65457few cells greater than 10 µm in length are observed
65457Na+ and Mg2+ ions are required for growth
65457predominant ubiquinone is Q-10

metabolite utilization

@refmetaboliteutilization activitykind of utilization testedChebi-ID
65457casein-hydrolysis
65457cellobiose-builds acid from17057
65457D-fructose-builds acid from15824
65457D-galactose-builds acid from12936
65457D-glucose-builds acid from17634
65457D-mannitol-builds acid from16899
65457D-mannose-builds acid from16024
65457D-ribose+builds acid from16988
65457D-sorbitol-builds acid from17924
65457D-xylose-builds acid from65327
65457esculin+hydrolysis4853
65457gelatin-hydrolysis5291
65457hypoxanthine-hydrolysis17368
65457L-arabinose+builds acid from30849
65457L-rhamnose-builds acid from62345
65457L-tyrosine-hydrolysis17895
65457lactose-builds acid from17716
65457maltose-builds acid from17306
65457melezitose-builds acid from6731
65457melibiose-builds acid from28053
65457myo-inositol-builds acid from17268
65457nitrate-reduction17632
65457raffinose-builds acid from16634
65457starch-hydrolysis28017
65457sucrose-builds acid from17992
65457trehalose-builds acid from27082
65457tween 20-hydrolysis53424
65457tween 40+hydrolysis53423
65457tween 60-hydrolysis53425
65457tween 80-hydrolysis53426
65457urea-hydrolysis16199
65457xanthine-hydrolysis15318

antibiotic resistance

@refChEBImetaboliteis antibioticis sensitivesensitivity conc.is resistantresistance conc.
6545728368novobiocinyesyes5 µg (disc)
6545718208penicillin gyesyes20 Unit (disc)
65457124991cefalotinyesyes30 µg (disc)
6545717833gentamicinyesyes30 µg (disc)
654576104kanamycinyesyes30 µg (disc)
654577507neomycinyesyes30 µg (disc)
6545727902tetracyclineyesyes30 µg (disc)
654573393carbenicillinyesyes100 µg (disc)
6545717698chloramphenicolyesyes100 µg (disc)
6545728971ampicillinyesyes10 µg (disc)
654576472lincomycinyesyes15 µg (disc)
6545716869oleandomycinyesyes15 µg (disc)
6545717076streptomycinyesyes50 µg (disc)
654578309polymyxin byesyes100 Unit (disc)

enzymes

@refvalueactivityec
65457alkaline phosphatase+3.1.3.1
65457esterase (C 4)+
65457esterase Lipase (C 8)+
65457leucine arylamidase+3.4.11.1
65457acid phosphatase+3.1.3.2
65457beta-glucosidase+3.2.1.21
65457naphthol-AS-BI-phosphohydrolase+
65457lipase (C 14)-
65457valine arylamidase-
65457cystine arylamidase-3.4.11.3
65457trypsin-3.4.21.4
65457alpha-chymotrypsin-3.4.21.1
65457alpha-galactosidase-3.2.1.22
65457beta-galactosidase-3.2.1.23
65457beta-glucuronidase-3.2.1.31
65457alpha-glucosidase-3.2.1.20
65457N-acetyl-beta-glucosaminidase-3.2.1.52
65457alpha-mannosidase-3.2.1.24
65457alpha-fucosidase-3.2.1.51
65457catalase+1.11.1.6
65457cytochrome oxidase+1.9.3.1

fatty acid profile

  • @reffatty acidpercentage
    65457C16:01.9
    65457C17:06.4
    65457C18:03
    65457C10:0 3OH2.3
    65457C11:0 3OH0.6
    65457C17:1ω6c1.4
    65457C17:1ω8c2
    65457C18:1ω7c70.7
    6545711-Methyl C18:1ω7c5.4
    65457Cyclo C19:0ω8c3.7
    65457C16:1ω7cand/or C16:1ω6c0.6
    65457C19:1ω6c and/or cyclo C19:0ω10c1.4
  • type of FA analysis: whole cell analysis
  • incubation medium: marine
  • agar/liquid: agar
  • incubation temperature: 30
  • incubation time: 3
  • software version: Sherlock 6.2B
  • library/peak naming table: TSBA6
  • system: MIS MIDI
  • instrument: Hewlett Packard 6890
  • method/protocol: Sasser, 1990
  • @reffatty acidpercentage
    65457C16:02.1
    65457C17:06.1
    65457C18:03.2
    65457C10:0 3OH2.3
    65457C11:0 3OH0.5
    65457C17:1ω6c1.3
    65457C17:1ω8c1.9
    65457C18:1ω7c67.2
    6545711-Methyl C18:1ω7c7.4
    65457Cyclo C19:0ω8c5.5
    65457C16:1ω7c and/or C16:1ω6c0.7
    65457C19:1ω6c and/or cyclo C19:0ω10c1.1
  • type of FA analysis: whole cell analysis
  • incubation medium: marine
  • agar/liquid: agar
  • incubation temperature: 30
  • incubation time: 5
  • software version: Sherlock 6.2B
  • library/peak naming table: TSBA6
  • system: MIS MIDI
  • instrument: Hewlett Packard 6890
  • method/protocol: Sasser, 1990
  • @reffatty acidpercentage
    65457C16:02.1
    65457C17:04.9
    65457C18:03.4
    65457C10:0 3OH2.5
    65457C17:1ω6c1
    65457C17:1ω8c1.6
    65457C18:1ω7c67.5
    6545711-Methyl C18:1ω7c7.4
    65457Cyclo C19:0ω8c6.8
    65457C16:1ω7c and/or C16:1ω6c0.7
    65457C19:1ω6c and/or cyclo C19:0ω10c1.5
  • type of FA analysis: whole cell analysis
  • incubation medium: marine
  • agar/liquid: agar
  • incubation temperature: 30
  • incubation time: 7
  • software version: Sherlock 6.2B
  • library/peak naming table: TSBA6
  • system: MIS MIDI
  • instrument: Hewlett Packard 6890
  • method/protocol: Sasser, 1990

Isolation, sampling and environmental information

isolation

  • @ref: 65457
  • sample type: a tidal flat sediment collected from Daebu island on the Yellow Sea
  • country: Republic of Korea
  • origin.country: KOR
  • continent: Asia
  • latitude: 37.2894
  • longitude: 126.577
  • enrichment culture: marine agar 2216
  • enrichment culture temperature: 25
  • isolation procedure: was isolated by the standard dilution plating technique on marine agar 2216 (MA; BD Difco) at 25C and cultivated routinely on MA at 30C unless stated otherwise.

isolation source categories

Cat1Cat2Cat3
#Environmental#Aquatic#Sediment
#Environmental#Terrestrial#Tidal flat

Sequence information

16S sequences

  • @ref: 65457
  • description: 16S rRNA gene sequence
  • accession: MG322243
  • database: nuccore

GC content

  • @ref: 65457
  • GC-content: 68.7
  • method: high performance liquid chromatography (HPLC)

External links

@ref: 65457

culture collection no.: KACC 19431, NBRC 113039

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny29504929Pseudomaribius aestuariivivens gen. nov., sp. nov., isolated from a tidal flat sediment.Park S, Park JM, Choi SJ, Choi J, Yoon JHInt J Syst Evol Microbiol10.1099/ijsem.0.0026772018Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Fatty Acids/chemistry, Geologic Sediments/*microbiology, Phosphatidylcholines/chemistry, Phosphatidylglycerols/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Republic of Korea, Rhodobacteraceae/*classification/genetics/isolation & purification, Seawater/*microbiology, Sequence Analysis, DNA, Ubiquinone/analogs & derivatives/chemistryTranscriptome
Phylogeny31733924Description of Palleronia rufa sp. nov., a biofilm-forming and AHL-producing Rhodobacteraceae, reclassification of Hwanghaeicola aestuarii as Palleronia aestuarii comb. nov., Maribius pontilimi as Palleronia pontilimi comb. nov., Maribius salinus as Palleronia salina comb. nov., Maribius pelagius as Palleronia pelagia comb. nov. and emended description of the genus Palleronia.Barnier C, Clerissi C, Lami R, Intertaglia L, Lebaron P, Grimaud R, Urios LSyst Appl Microbiol10.1016/j.syapm.2019.1260182019Acyl-Butyrolactones/*metabolism, Bacterial Proteins/genetics, Biofilms/*growth & development, DNA, Bacterial/genetics, Genes, Essential/genetics, Genome, Bacterial/genetics, New Caledonia, Phylogeny, Quorum Sensing, RNA, Ribosomal, 16S/genetics, Rhodobacteraceae/chemistry/*classification/cytology/*physiology, Roseobacter/chemistry/classification/cytology/physiology, Seawater/microbiology, Sequence Analysis, DNA, Sequence Homology, Nucleic Acid, Species SpecificityGenetics

Reference

@idauthorstitledoi/urljournalpubmed
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
65457Sooyeon Park, Ji-Min Park, Su Jung Choi, Jeehyun Choi, Jung-Hoon YoonPseudomaribius aestuariivivens gen. nov., sp. nov., isolated from a tidal flat sediment10.1099/ijsem.0.002677IJSEM 68: 1344-1349 201829504929