Strain identifier
BacDive ID: 158715
Type strain:
Species: Pseudomaribius aestuariivivens
Strain Designation: DBTF-15
NCBI tax ID(s): 2050951 (species)
General
@ref: 65457
BacDive-ID: 158715
keywords: 16S sequence, Bacteria, obligate aerobe, mesophilic, Gram-negative, rod-shaped, colony-forming
description: Pseudomaribius aestuariivivens DBTF-15 is an obligate aerobe, mesophilic, Gram-negative bacterium that forms circular colonies and was isolated from a tidal flat sediment collected from Daebu island on the Yellow Sea.
NCBI tax id
- NCBI tax id: 2050951
- Matching level: species
doi: 10.13145/bacdive158715.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/pseudomonadota
- domain: Bacteria
- phylum: Pseudomonadota
- class: Alphaproteobacteria
- order: Rhodobacterales
- family: Roseobacteraceae
- genus: Pseudomaribius
- species: Pseudomaribius aestuariivivens
- full scientific name: Pseudomaribius aestuariivivens Park et al. 2018
@ref: 65457
domain: Bacteria
phylum: Proteobacteria
class: Alphaproteobacteria
order: Rhodobacterales
family: Rhodobacteraceae
genus: Pseudomaribius
species: Pseudomaribius aestuariivivens
strain designation: DBTF-15
type strain: yes
Morphology
cell morphology
- @ref: 65457
- gram stain: negative
- cell length: 0.3-1.6 µm
- cell width: 0.5-1.0 µm
- cell shape: rod-shaped
- motility: no
colony morphology
- @ref: 65457
- colony size: 0.5-0.8 mm
- colony color: greyish yellow
- colony shape: circular
- incubation period: 7 days
- medium used: marine agar
Culture and growth conditions
culture medium
- @ref: 65457
- name: Marine agar (MA)
- growth: yes
culture temp
@ref | growth | type | temperature | range |
---|---|---|---|---|
65457 | positive | growth | 30 | mesophilic |
65457 | positive | optimum | 25-30 | mesophilic |
65457 | positive | growth | 10 | psychrophilic |
65457 | positive | growth | 37 | mesophilic |
65457 | no | growth | 40 | thermophilic |
65457 | no | growth | 4 | psychrophilic |
culture pH
@ref | ability | type | pH |
---|---|---|---|
65457 | positive | optimum | 7-8 |
65457 | positive | growth | 6 |
65457 | no | growth | 5.5 |
Physiology and metabolism
oxygen tolerance
- @ref: 65457
- oxygen tolerance: obligate aerobe
spore formation
- @ref: 65457
- spore formation: no
halophily
@ref | salt | growth | tested relation | concentration |
---|---|---|---|---|
65457 | NaCl | positive | growth | 0.5-5.0 %(w/v) |
65457 | NaCl | positive | optimum | 2 %(w/v) |
observation
@ref | observation |
---|---|
65457 | few cells greater than 10 µm in length are observed |
65457 | Na+ and Mg2+ ions are required for growth |
65457 | predominant ubiquinone is Q-10 |
metabolite utilization
@ref | metabolite | utilization activity | kind of utilization tested | Chebi-ID |
---|---|---|---|---|
65457 | casein | - | hydrolysis | |
65457 | cellobiose | - | builds acid from | 17057 |
65457 | D-fructose | - | builds acid from | 15824 |
65457 | D-galactose | - | builds acid from | 12936 |
65457 | D-glucose | - | builds acid from | 17634 |
65457 | D-mannitol | - | builds acid from | 16899 |
65457 | D-mannose | - | builds acid from | 16024 |
65457 | D-ribose | + | builds acid from | 16988 |
65457 | D-sorbitol | - | builds acid from | 17924 |
65457 | D-xylose | - | builds acid from | 65327 |
65457 | esculin | + | hydrolysis | 4853 |
65457 | gelatin | - | hydrolysis | 5291 |
65457 | hypoxanthine | - | hydrolysis | 17368 |
65457 | L-arabinose | + | builds acid from | 30849 |
65457 | L-rhamnose | - | builds acid from | 62345 |
65457 | L-tyrosine | - | hydrolysis | 17895 |
65457 | lactose | - | builds acid from | 17716 |
65457 | maltose | - | builds acid from | 17306 |
65457 | melezitose | - | builds acid from | 6731 |
65457 | melibiose | - | builds acid from | 28053 |
65457 | myo-inositol | - | builds acid from | 17268 |
65457 | nitrate | - | reduction | 17632 |
65457 | raffinose | - | builds acid from | 16634 |
65457 | starch | - | hydrolysis | 28017 |
65457 | sucrose | - | builds acid from | 17992 |
65457 | trehalose | - | builds acid from | 27082 |
65457 | tween 20 | - | hydrolysis | 53424 |
65457 | tween 40 | + | hydrolysis | 53423 |
65457 | tween 60 | - | hydrolysis | 53425 |
65457 | tween 80 | - | hydrolysis | 53426 |
65457 | urea | - | hydrolysis | 16199 |
65457 | xanthine | - | hydrolysis | 15318 |
antibiotic resistance
@ref | ChEBI | metabolite | is antibiotic | is sensitive | sensitivity conc. | is resistant | resistance conc. |
---|---|---|---|---|---|---|---|
65457 | 28368 | novobiocin | yes | yes | 5 µg (disc) | ||
65457 | 18208 | penicillin g | yes | yes | 20 Unit (disc) | ||
65457 | 124991 | cefalotin | yes | yes | 30 µg (disc) | ||
65457 | 17833 | gentamicin | yes | yes | 30 µg (disc) | ||
65457 | 6104 | kanamycin | yes | yes | 30 µg (disc) | ||
65457 | 7507 | neomycin | yes | yes | 30 µg (disc) | ||
65457 | 27902 | tetracycline | yes | yes | 30 µg (disc) | ||
65457 | 3393 | carbenicillin | yes | yes | 100 µg (disc) | ||
65457 | 17698 | chloramphenicol | yes | yes | 100 µg (disc) | ||
65457 | 28971 | ampicillin | yes | yes | 10 µg (disc) | ||
65457 | 6472 | lincomycin | yes | yes | 15 µg (disc) | ||
65457 | 16869 | oleandomycin | yes | yes | 15 µg (disc) | ||
65457 | 17076 | streptomycin | yes | yes | 50 µg (disc) | ||
65457 | 8309 | polymyxin b | yes | yes | 100 Unit (disc) |
enzymes
@ref | value | activity | ec |
---|---|---|---|
65457 | alkaline phosphatase | + | 3.1.3.1 |
65457 | esterase (C 4) | + | |
65457 | esterase Lipase (C 8) | + | |
65457 | leucine arylamidase | + | 3.4.11.1 |
65457 | acid phosphatase | + | 3.1.3.2 |
65457 | beta-glucosidase | + | 3.2.1.21 |
65457 | naphthol-AS-BI-phosphohydrolase | + | |
65457 | lipase (C 14) | - | |
65457 | valine arylamidase | - | |
65457 | cystine arylamidase | - | 3.4.11.3 |
65457 | trypsin | - | 3.4.21.4 |
65457 | alpha-chymotrypsin | - | 3.4.21.1 |
65457 | alpha-galactosidase | - | 3.2.1.22 |
65457 | beta-galactosidase | - | 3.2.1.23 |
65457 | beta-glucuronidase | - | 3.2.1.31 |
65457 | alpha-glucosidase | - | 3.2.1.20 |
65457 | N-acetyl-beta-glucosaminidase | - | 3.2.1.52 |
65457 | alpha-mannosidase | - | 3.2.1.24 |
65457 | alpha-fucosidase | - | 3.2.1.51 |
65457 | catalase | + | 1.11.1.6 |
65457 | cytochrome oxidase | + | 1.9.3.1 |
fatty acid profile
@ref fatty acid percentage 65457 C16:0 1.9 65457 C17:0 6.4 65457 C18:0 3 65457 C10:0 3OH 2.3 65457 C11:0 3OH 0.6 65457 C17:1ω6c 1.4 65457 C17:1ω8c 2 65457 C18:1ω7c 70.7 65457 11-Methyl C18:1ω7c 5.4 65457 Cyclo C19:0ω8c 3.7 65457 C16:1ω7cand/or C16:1ω6c 0.6 65457 C19:1ω6c and/or cyclo C19:0ω10c 1.4 - type of FA analysis: whole cell analysis
- incubation medium: marine
- agar/liquid: agar
- incubation temperature: 30
- incubation time: 3
- software version: Sherlock 6.2B
- library/peak naming table: TSBA6
- system: MIS MIDI
- instrument: Hewlett Packard 6890
- method/protocol: Sasser, 1990
@ref fatty acid percentage 65457 C16:0 2.1 65457 C17:0 6.1 65457 C18:0 3.2 65457 C10:0 3OH 2.3 65457 C11:0 3OH 0.5 65457 C17:1ω6c 1.3 65457 C17:1ω8c 1.9 65457 C18:1ω7c 67.2 65457 11-Methyl C18:1ω7c 7.4 65457 Cyclo C19:0ω8c 5.5 65457 C16:1ω7c and/or C16:1ω6c 0.7 65457 C19:1ω6c and/or cyclo C19:0ω10c 1.1 - type of FA analysis: whole cell analysis
- incubation medium: marine
- agar/liquid: agar
- incubation temperature: 30
- incubation time: 5
- software version: Sherlock 6.2B
- library/peak naming table: TSBA6
- system: MIS MIDI
- instrument: Hewlett Packard 6890
- method/protocol: Sasser, 1990
@ref fatty acid percentage 65457 C16:0 2.1 65457 C17:0 4.9 65457 C18:0 3.4 65457 C10:0 3OH 2.5 65457 C17:1ω6c 1 65457 C17:1ω8c 1.6 65457 C18:1ω7c 67.5 65457 11-Methyl C18:1ω7c 7.4 65457 Cyclo C19:0ω8c 6.8 65457 C16:1ω7c and/or C16:1ω6c 0.7 65457 C19:1ω6c and/or cyclo C19:0ω10c 1.5 - type of FA analysis: whole cell analysis
- incubation medium: marine
- agar/liquid: agar
- incubation temperature: 30
- incubation time: 7
- software version: Sherlock 6.2B
- library/peak naming table: TSBA6
- system: MIS MIDI
- instrument: Hewlett Packard 6890
- method/protocol: Sasser, 1990
Isolation, sampling and environmental information
isolation
- @ref: 65457
- sample type: a tidal flat sediment collected from Daebu island on the Yellow Sea
- country: Republic of Korea
- origin.country: KOR
- continent: Asia
- latitude: 37.2894
- longitude: 126.577
- enrichment culture: marine agar 2216
- enrichment culture temperature: 25
- isolation procedure: was isolated by the standard dilution plating technique on marine agar 2216 (MA; BD Difco) at 25C and cultivated routinely on MA at 30C unless stated otherwise.
isolation source categories
Cat1 | Cat2 | Cat3 |
---|---|---|
#Environmental | #Aquatic | #Sediment |
#Environmental | #Terrestrial | #Tidal flat |
Sequence information
16S sequences
- @ref: 65457
- description: 16S rRNA gene sequence
- accession: MG322243
- database: nuccore
GC content
- @ref: 65457
- GC-content: 68.7
- method: high performance liquid chromatography (HPLC)
External links
@ref: 65457
culture collection no.: KACC 19431, NBRC 113039
literature
topic | Pubmed-ID | title | authors | journal | DOI | year | mesh | topic2 |
---|---|---|---|---|---|---|---|---|
Phylogeny | 29504929 | Pseudomaribius aestuariivivens gen. nov., sp. nov., isolated from a tidal flat sediment. | Park S, Park JM, Choi SJ, Choi J, Yoon JH | Int J Syst Evol Microbiol | 10.1099/ijsem.0.002677 | 2018 | Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Fatty Acids/chemistry, Geologic Sediments/*microbiology, Phosphatidylcholines/chemistry, Phosphatidylglycerols/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Republic of Korea, Rhodobacteraceae/*classification/genetics/isolation & purification, Seawater/*microbiology, Sequence Analysis, DNA, Ubiquinone/analogs & derivatives/chemistry | Transcriptome |
Phylogeny | 31733924 | Description of Palleronia rufa sp. nov., a biofilm-forming and AHL-producing Rhodobacteraceae, reclassification of Hwanghaeicola aestuarii as Palleronia aestuarii comb. nov., Maribius pontilimi as Palleronia pontilimi comb. nov., Maribius salinus as Palleronia salina comb. nov., Maribius pelagius as Palleronia pelagia comb. nov. and emended description of the genus Palleronia. | Barnier C, Clerissi C, Lami R, Intertaglia L, Lebaron P, Grimaud R, Urios L | Syst Appl Microbiol | 10.1016/j.syapm.2019.126018 | 2019 | Acyl-Butyrolactones/*metabolism, Bacterial Proteins/genetics, Biofilms/*growth & development, DNA, Bacterial/genetics, Genes, Essential/genetics, Genome, Bacterial/genetics, New Caledonia, Phylogeny, Quorum Sensing, RNA, Ribosomal, 16S/genetics, Rhodobacteraceae/chemistry/*classification/cytology/*physiology, Roseobacter/chemistry/classification/cytology/physiology, Seawater/microbiology, Sequence Analysis, DNA, Sequence Homology, Nucleic Acid, Species Specificity | Genetics |
Reference
@id | authors | title | doi/url | journal | pubmed |
---|---|---|---|---|---|
20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | 10.1099/ijsem.0.004332 | ||
65457 | Sooyeon Park, Ji-Min Park, Su Jung Choi, Jeehyun Choi, Jung-Hoon Yoon | Pseudomaribius aestuariivivens gen. nov., sp. nov., isolated from a tidal flat sediment | 10.1099/ijsem.0.002677 | IJSEM 68: 1344-1349 2018 | 29504929 |