Strain identifier
BacDive ID: 158688
Type strain:
Species: Planococcus salinus
Strain Designation: LCB217
Strain history: <- Longzhan GAN, Sichuan Univ.
NCBI tax ID(s): 1848460 (species)
General
@ref: 65424
BacDive-ID: 158688
keywords: genome sequence, 16S sequence, Bacteria, aerobe, moderately halophilic, Gram-positive, motile, coccus-shaped, colony-forming
description: Planococcus salinus LCB217 is an aerobe, moderately halophilic, Gram-positive bacterium that forms circular colonies and was isolated from a saline-alkali soil of Jade Gate Pass.
NCBI tax id
- NCBI tax id: 1848460
- Matching level: species
strain history
- @ref: 67771
- history: <- Longzhan GAN, Sichuan Univ.
doi: 10.13145/bacdive158688.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/bacillota
- domain: Bacteria
- phylum: Bacillota
- class: Bacilli
- order: Caryophanales
- family: Caryophanaceae
- genus: Planococcus
- species: Planococcus salinus
- full scientific name: Planococcus salinus Gan et al. 2018
@ref: 65424
domain: Bacteria
phylum: Firmicutes
class: Bacilli
order: Caryophanales
family: Planococcaceae
genus: Planococcus
species: Planococcus salinus
strain designation: LCB217
type strain: yes
Morphology
cell morphology
@ref | gram stain | cell width | cell shape | motility |
---|---|---|---|---|
65424 | positive | 0.6-1.0 µm | coccus-shaped | yes |
67771 | coccus-shaped | yes | ||
67771 | positive |
colony morphology
- @ref: 65424
- colony size: 1-2 mm
- colony color: reddish-orange
- colony shape: circular
- incubation period: 3 days
- medium used: LB
Culture and growth conditions
culture medium
- @ref: 65424
- name: LB (Luria-Bertani) MEDIUM
- growth: yes
culture temp
@ref | growth | type | temperature | range |
---|---|---|---|---|
65424 | positive | growth | 10-45 | |
65424 | positive | optimum | 30 | mesophilic |
67771 | positive | growth | 30 | mesophilic |
culture pH
@ref | ability | type | pH | PH range |
---|---|---|---|---|
65424 | positive | growth | 7-9 | alkaliphile |
65424 | positive | optimum | 9 |
Physiology and metabolism
oxygen tolerance
@ref | oxygen tolerance |
---|---|
65424 | aerobe |
67771 | aerobe |
halophily
@ref | halophily level | salt | growth | tested relation | concentration |
---|---|---|---|---|---|
65424 | moderately halophilic | ||||
65424 | NaCl | positive | growth | 3-15 % | |
65424 | NaCl | positive | optimum | 3-5 % |
observation
@ref | observation |
---|---|
65424 | the peptidoglycan type is A4a (L-Lys-D-Glu) |
65424 | the predominant menaquinones are MK-7 and MK-8 |
65424 | the polar lipids are diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine, one unidentified phospholipid, one unidentified aminophospholipid and one unidentified lipid |
67771 | quinones: MK-7, MK-8 |
metabolite utilization
@ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
---|---|---|---|---|
65424 | 18287 | L-fucose | + | carbon source |
65424 | 16004 | (R)-lactate | + | carbon source |
65424 | 15588 | D-malate | + | carbon source |
65424 | 16651 | (S)-lactate | - | carbon source |
65424 | 73918 | 3-O-methyl-D-glucose | + | carbon source |
65424 | 18101 | 4-hydroxyphenylacetic acid | - | carbon source |
65424 | 30089 | acetate | + | carbon source |
65424 | 17925 | alpha-D-glucose | + | carbon source |
65424 | 64552 | 2-hydroxybutyrate | - | carbon source |
65424 | 73673 | alpha-hydroxyglutarate-gamma-lactone | - | carbon source |
65424 | 8295 | beta-hydroxybutyrate | + | carbon source |
65424 | 73706 | bromosuccinate | - | carbon source |
65424 | 17057 | cellobiose | - | builds acid from |
65424 | 62968 | cellulose | - | hydrolysis |
65424 | 16947 | citrate | - | carbon source |
65424 | 18333 | D-arabitol | + | carbon source |
65424 | 29990 | D-aspartate | - | carbon source |
65424 | 17057 | cellobiose | + | carbon source |
65424 | 15824 | D-fructose | + | carbon source |
65424 | 78697 | D-fructose 6-phosphate | + | carbon source |
65424 | 28847 | D-fucose | + | carbon source |
65424 | 12936 | D-galactose | - | builds acid from |
65424 | 12936 | D-galactose | + | carbon source |
65424 | 18024 | D-galacturonic acid | - | carbon source |
65424 | 8391 | D-gluconate | - | carbon source |
65424 | 14314 | D-glucose 6-phosphate | - | carbon source |
65424 | 15748 | D-glucuronate | - | carbon source |
65424 | 16899 | D-mannitol | - | builds acid from |
65424 | 16899 | D-mannitol | + | carbon source |
65424 | 16024 | D-mannose | + | carbon source |
65424 | 33801 | D-saccharate | - | carbon source |
65424 | 16523 | D-serine | - | carbon source |
65424 | 17924 | D-sorbitol | - | builds acid from |
65424 | 17924 | D-sorbitol | + | carbon source |
65424 | 15740 | formate | + | carbon source |
65424 | 28757 | fructose | - | builds acid from |
65424 | 16865 | gamma-aminobutyric acid | - | carbon source |
65424 | 495056 | gamma-cyclodextrin | + | carbon source |
65424 | 5291 | gelatin | - | carbon source |
65424 | 5291 | gelatin | - | hydrolysis |
65424 | 28066 | gentiobiose | + | carbon source |
65424 | 17234 | glucose | - | builds acid from |
65424 | 32323 | glucuronamide | + | carbon source |
65424 | 17754 | glycerol | + | carbon source |
65424 | 70744 | glycine-proline | - | carbon source |
65424 | 17596 | inosine | + | carbon source |
65424 | L-alanine 4-nitroanilide | - | carbon source | |
65424 | 16467 | L-arginine | - | carbon source |
65424 | 29991 | L-aspartate | - | carbon source |
65424 | 17464 | L-galactonic acid gamma-lactone | + | carbon source |
65424 | 29985 | L-glutamate | - | carbon source |
65424 | 15971 | L-histidine | + | carbon source |
65424 | 15589 | L-malate | - | carbon source |
65424 | 18183 | L-pyroglutamic acid | - | carbon source |
65424 | 62345 | L-rhamnose | + | carbon source |
65424 | 17115 | L-serine | - | carbon source |
65424 | 17716 | lactose | + | carbon source |
65424 | 17306 | maltose | - | builds acid from |
65424 | 17306 | maltose | + | carbon source |
65424 | 28053 | melibiose | + | carbon source |
65424 | 37657 | methyl D-glucoside | + | carbon source |
65424 | 51850 | methyl pyruvate | - | carbon source |
65424 | 17268 | myo-inositol | + | carbon source |
65424 | 63154 | N-acetyl-beta-D-mannosamine | + | carbon source |
65424 | 28037 | N-acetylgalactosamine | + | carbon source |
65424 | 506227 | N-acetylglucosamine | + | carbon source |
65424 | 35418 | n-acetylneuraminate | - | carbon source |
65424 | 17632 | nitrate | + | reduction |
65424 | 17309 | pectin | - | carbon source |
65424 | 17272 | propionate | + | carbon source |
65424 | 26490 | quinate | - | carbon source |
65424 | 16634 | raffinose | + | carbon source |
65424 | 17814 | salicin | + | carbon source |
65424 | 17164 | stachyose | + | carbon source |
65424 | 28017 | starch | - | hydrolysis |
65424 | 17992 | sucrose | - | builds acid from |
65424 | 17992 | sucrose | + | carbon source |
65424 | 27082 | trehalose | - | builds acid from |
65424 | 27082 | trehalose | + | carbon source |
65424 | 32528 | turanose | + | carbon source |
65424 | 53424 | tween 20 | + | hydrolysis |
65424 | 53423 | tween 40 | + | carbon source |
65424 | 53423 | tween 40 | + | hydrolysis |
65424 | 53425 | tween 60 | + | hydrolysis |
65424 | 53426 | tween 80 | - | hydrolysis |
65424 | 16199 | urea | - | hydrolysis |
metabolite production
@ref | Chebi-ID | metabolite | production |
---|---|---|---|
65424 | 35581 | indole | yes |
65424 | 15138 | sulfide | no |
metabolite tests
@ref | Chebi-ID | metabolite | methylred-test | voges-proskauer-test |
---|---|---|---|---|
65424 | 17234 | glucose | - | |
65424 | 15688 | acetoin | - |
enzymes
@ref | value | activity | ec |
---|---|---|---|
65424 | cytochrome oxidase | + | 1.9.3.1 |
65424 | alkaline phosphatase | + | 3.1.3.1 |
65424 | esterase (C 4) | + | |
65424 | esterase Lipase (C 8) | + | |
65424 | leucine arylamidase | + | 3.4.11.1 |
65424 | cystine arylamidase | + | 3.4.11.3 |
65424 | valine arylamidase | + | |
65424 | alpha-chymotrypsin | + | 3.4.21.1 |
65424 | lipase (C 14) | + | |
65424 | trypsin | + | 3.4.21.4 |
65424 | acid phosphatase | + | 3.1.3.2 |
65424 | naphthol-AS-BI-phosphohydrolase | + | |
65424 | alpha-galactosidase | - | 3.2.1.22 |
65424 | beta-galactosidase | - | 3.2.1.23 |
65424 | beta-glucuronidase | - | 3.2.1.31 |
65424 | alpha-glucosidase | - | 3.2.1.20 |
65424 | beta-glucosidase | - | 3.2.1.21 |
65424 | N-acetyl-beta-glucosaminidase | - | 3.2.1.52 |
65424 | alpha-mannosidase | - | 3.2.1.24 |
65424 | alpha-fucosidase | - | 3.2.1.51 |
Isolation, sampling and environmental information
isolation
@ref | sample type | geographic location | country | origin.country | continent | enrichment culture | enrichment culture composition | enrichment culture duration | enrichment culture temperature |
---|---|---|---|---|---|---|---|---|---|
65424 | a saline-alkali soil of Jade Gate Pass | Gansu Province | China | CHN | Asia | saline agar medium | per litre, 7.5 g casein peptone, 100 g NaCl, 10 g yeast extract, 1.5 ml MS mixture (0.05 g each of betaine, proline, glycine, D-sorbitol and glutamate, added to 1000 ml H 2 O), 20 g agar (adjusted to pH 8.0 with HCl/NaOH) | 3 days | 37 |
67771 | From saline-alkali soil of Jade Gate Pass | Gansu Province | China | CHN | Asia |
isolation source categories
Cat1 | Cat2 | Cat3 |
---|---|---|
#Environmental | #Terrestrial | #Soil |
#Condition | #Alkaline | |
#Condition | #Saline |
Sequence information
16S sequences
- @ref: 65424
- description: 16S rRNA gene sequence
- accession: KX008965
- database: nuccore
Genome sequences
@ref | description | accession | assembly level | database | NCBI tax ID |
---|---|---|---|---|---|
66792 | Planococcus salinus LCB217 | GCA_003719725 | contig | ncbi | 1848460 |
66792 | Planococcus salinus LCB217 | 2843378535 | draft | img | 1848460 |
GC content
@ref | GC-content | method |
---|---|---|
65424 | 49.4 | thermal denaturation, midpoint method (Tm) |
67771 | 49.4 |
External links
@ref: 65424
culture collection no.: CGMCC 1.15685, KCTC 33861
literature
topic | Pubmed-ID | title | authors | journal | DOI | year | mesh | topic2 |
---|---|---|---|---|---|---|---|---|
Phylogeny | 29300160 | Planococcus salinus sp. nov., a moderately halophilic bacterium isolated from a saline-alkali soil. | Gan L, Zhang H, Tian J, Li X, Long X, Zhang Y, Dai Y, Tian Y | Int J Syst Evol Microbiol | 10.1099/ijsem.0.002548 | 2018 | Alkalies, Bacterial Typing Techniques, Base Composition, China, DNA, Bacterial/genetics, Fatty Acids/chemistry, Nucleic Acid Hybridization, Phospholipids/chemistry, *Phylogeny, Planococcus Bacteria/*classification/genetics/isolation & purification, RNA, Ribosomal, 16S/genetics, Salinity, Sequence Analysis, DNA, Soil/chemistry, *Soil Microbiology, Vitamin K 2/analogs & derivatives/chemistry | Transcriptome |
Phylogeny | 31287396 | Indiicoccus explosivorum gen. nov., sp. nov., isolated from an explosives waste contaminated site. | Pal Y, Mayilraj S, Paul M, Schumann P, Krishnamurthi S | Int J Syst Evol Microbiol | 10.1099/ijsem.0.003541 | 2019 | Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, *Explosive Agents, Fatty Acids/chemistry, India, Peptidoglycan/chemistry, Phosphatidylethanolamines, *Phylogeny, Pigmentation, Planococcaceae/*classification/isolation & purification, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, *Soil Pollutants, Vitamin K 2/chemistry | Transcriptome |
Reference
@id | authors | title | doi/url | journal | pubmed |
---|---|---|---|---|---|
20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | 10.1099/ijsem.0.004332 | ||
65424 | Longzhan Gan, Heming Zhang, Jiewei Tian, Xiaoguang Li, Xiufeng Long, Yuqin Zhang, Yumei Dai, Yongqiang Tian | Planococcus salinus sp. nov., a moderately halophilic bacterium isolated from a saline-alkali soil | 10.1099/ijsem.0.002548 | IJSEM 68: 589-595 2018 | 29300160 |
66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | https://diaspora-project.de/progress.html#genomes | ||
66794 | Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg | BRENDA, the ELIXIR core data resource in 2021: new developments and updates | 10.1093/nar/gkaa1025 | Nucleic Acids Res. 49: D498-D508 2020 | 33211880 |
67771 | Curators of the KCTC | https://kctc.kribb.re.kr/En/Kctc |