Strain identifier

BacDive ID: 158675

Type strain: Yes

Species: Marinobacter salexigens

NCBI tax ID(s): 1925763 (species)

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General

@ref: 65407

BacDive-ID: 158675

keywords: 16S sequence, Bacteria, facultative anaerobe, moderately halophilic, Gram-negative, rod-shaped, colony-forming

description: Marinobacter salexigens KCTC 52545 is a facultative anaerobe, moderately halophilic, Gram-negative bacterium that forms circular colonies and was isolated from the coastal area.

NCBI tax id

  • NCBI tax id: 1925763
  • Matching level: species

doi: 10.13145/bacdive158675.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Alteromonadales
  • family: Alteromonadaceae
  • genus: Marinobacter
  • species: Marinobacter salexigens
  • full scientific name: Marinobacter salexigens Han et al. 2017

@ref: 65407

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Alteromonadales

family: Alteromonadaceae

genus: Marinobacter

species: Marinobacter salexigens

type strain: yes

Morphology

cell morphology

  • @ref: 65407
  • gram stain: negative
  • cell length: 2.0-2.5 µm
  • cell width: 0.3-0.5 µm
  • cell shape: rod-shaped
  • motility: no

colony morphology

  • @ref: 65407
  • colony size: 0.5-1.5 mm
  • colony color: creamy white
  • colony shape: circular
  • incubation period: 3 days
  • medium used: marine agar 2216

Culture and growth conditions

culture medium

  • @ref: 65407
  • name: Marine agar (MA)
  • growth: yes

culture temp

@refgrowthtypetemperaturerange
65407positivegrowth4-40
65407positiveoptimum30mesophilic

culture pH

@refabilitytypepHPH range
65407positivegrowth6.5-9.5alkaliphile
65407positiveoptimum7.0-7.5

Physiology and metabolism

oxygen tolerance

  • @ref: 65407
  • oxygen tolerance: facultative anaerobe

halophily

@refhalophily levelsaltgrowthtested relationconcentration
65407moderately halophilic
65407NaClpositivegrowth0.5-15.0 %(w/v)
65407NaClpositiveoptimum3.0 %(w/v)

observation

@refobservation
65407colonies are shiny with entire margins
65407ubiquinone-9 (Q-9) is the predominant quinone
65407major polar lipids are phosphatidylglycerol, phosphatidylethanolamine, and an unidentified phospholipid

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6540717108D-arabinose-carbon source
6540748095(-)-D-fructose+builds acid from
6540712936D-galactose+carbon source
6540765327D-xylose-carbon source
6540729985L-glutamate-carbon source
6540729985L-glutamate+oxidation
65407645522-hydroxybutyrate+oxidation
65407167244-hydroxybutyrate+oxidation
6540730089acetate+oxidation
654072509agar-assimilation
65407495055beta-cyclodextrin-carbon source
6540773706bromosuccinate+oxidation
65407casein-assimilation
6540762968cellulose-assimilation
6540723456cyclodextrin-builds acid from
6540717108D-arabinose+builds acid from
6540715824D-fructose+carbon source
6540715824D-fructose+oxidation
6540778697D-fructose 6-phosphate+oxidation
6540712936D-galactose-builds acid from
6540718024D-galacturonic acid+oxidation
654078391D-gluconate+oxidation
6540716899D-mannitol-builds acid from
6540716899D-mannitol-carbon source
6540716024D-mannose-builds acid from
6540716024D-mannose-carbon source
6540716988D-ribose-carbon source
6540716988D-ribose+builds acid from
6540765327D-xylose+builds acid from
6540716991dna-assimilation
6540717234glucose-builds acid from
6540717234glucose+carbon source
6540732323glucuronamide+oxidation
6540717754glycerol+oxidation
6540715428glycine-builds acid from
6540715428glycine-carbon source
6540770744glycine-proline+oxidation
6540716087isocitrate+assimilation
65407L-alanine 4-nitroanilide+builds acid from
65407L-alanine 4-nitroanilide+carbon source
65407L-alanine 4-nitroanilide+oxidation
6540716467L-arginine-builds acid from
6540716467L-arginine-carbon source
6540717561L-cysteine+builds acid from
6540790426L-cysteine 2-naphthylamide+carbon source
6540716283L-cystine-assimilation
6540717464L-galactonic acid gamma-lactone+oxidation
6540729985L-glutamate-builds acid from
6540717295L-phenylalanine+builds acid from
6540717295L-phenylalanine+carbon source
6540716828L-tryptophan-builds acid from
6540716828L-tryptophan-carbon source
6540717895L-tyrosine+builds acid from
6540724996lactate+oxidation
6540717716lactose-builds acid from
6540717716lactose-carbon source
6540725115malate+oxidation
65407maltose-builds acid from
6540717306maltose-builds acid from
6540717306maltose-carbon source
6540751850methyl pyruvate+oxidation
6540717268myo-inositol-builds acid from
6540717268myo-inositol-carbon source
6540716301nitrite-reduction
6540763043potassium nitrate+growth
6540717272propionate+oxidation
6540716634raffinose-builds acid from
6540716634raffinose-carbon source
6540717164stachyose+oxidation
6540728017starch-assimilation
6540728017starch-builds acid from
6540728017starch+carbon source
6540717992sucrose-builds acid from
6540717992sucrose-carbon source
6540727082trehalose-builds acid from
6540727082trehalose-carbon source
6540753424tween 20-hydrolysis
6540753423tween 40-hydrolysis
6540753423tween 40+oxidation
6540753425tween 60-hydrolysis
6540753426tween 80-hydrolysis
6540718186tyrosine-assimilation

metabolite production

@refChebi-IDmetaboliteproduction
6540735581indoleno
6540715138sulfideno

metabolite tests

  • @ref: 65407
  • Chebi-ID: 15688
  • metabolite: acetoin
  • voges-proskauer-test: -

enzymes

@refvalueactivityec
65407cytochrome oxidase+1.9.3.1
65407catalase+1.11.1.6
65407alkaline phosphatase+3.1.3.1
65407leucine arylamidase+3.4.11.1
65407esterase (C 4)+
65407esterase Lipase (C 8)+
65407lipase (C 14)+
65407N-acetyl-beta-glucosaminidase+3.2.1.52
65407valine arylamidase+
65407naphthol-AS-BI-phosphohydrolase+
65407urease+3.5.1.5
65407gelatinase-
65407amylase-
65407lysine decarboxylase-4.1.1.18
65407ornithine decarboxylase-4.1.1.17
65407arginine dihydrolase-3.5.3.6
65407tyrosine arylamidase-

fatty acid profile

  • fatty acids

    @reffatty acidpercentage
    65407C12:011.4
    65407C12:0 3OH3.1
    65407C16:014.8
    65407C16:0 N alcohol12.3
    65407C16:0 10-methyl6
    65407C16:1ω7c alcohol6.6
    65407C16:1ω9c1.8
    65407C 17 : 0 10-methyl2.6
    65407C17:1ω8c1.1
    65407C18:01.4
    65407C18:1ω9c13.4
    65407C18:1ω7c1.3
    65407C18:3ω6,9,12c13.1
    65407C16:1ω7c and/or iso-C 15:0 2-OH10
  • type of FA analysis: whole cell analysis
  • incubation medium: marine agar
  • incubation temperature: 30
  • incubation time: 2
  • software version: Sherlock 4.5
  • library/peak naming table: TSBA40
  • system: MIS MIDI
  • instrument: 6890N gas chromatograph (Agilent)
  • method/protocol: Sasser, 1990
  • cutoff value:

Isolation, sampling and environmental information

isolation

  • @ref: 65407
  • sample type: the coastal area
  • geographic location: Weihai
  • country: China
  • origin.country: CHN
  • continent: Asia
  • latitude: 37.4833
  • longitude: 121.95

isolation source categories

  • Cat1: #Environmental
  • Cat2: #Terrestrial
  • Cat3: #Coast

Sequence information

16S sequences

  • @ref: 65407
  • description: 16S rRNA gene sequence
  • accession: KX839493
  • database: nuccore

GC content

  • @ref: 65407
  • GC-content: 53.7
  • method: high performance liquid chromatography (HPLC)

External links

@ref: 65407

culture collection no.: KCTC 52545, MCCC 1H00176, HJR 7

literature

  • topic: Phylogeny
  • Pubmed-ID: 28945532
  • title: Marinobacter salexigens sp. nov., isolated from marine sediment.
  • authors: Han JR, Ling SK, Yu WN, Chen GJ, Du ZJ
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijsem.0.002337
  • year: 2017
  • mesh: Bacterial Typing Techniques, Base Composition, China, DNA, Bacterial/genetics, Fatty Acids/chemistry, Geologic Sediments/*microbiology, Marinobacter/*classification/genetics/isolation & purification, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Seawater/*microbiology, Sequence Analysis, DNA, Ubiquinone/chemistry
  • topic2: Transcriptome

Reference

@idauthorstitledoi/urljournalpubmed
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
65407Ji-Ru Han, Si-Kai Ling, Wen-Nan Yu, Guan-Jun Chen, Zong-Jun DuMarinobacter salexigens sp. nov., isolated from marine sediment10.1099/ijsem.0.002337IJSEM 67: 4595-4600 201728945532