Strain identifier

BacDive ID: 158666

Type strain: Yes

Species: Streptomyces manganisoli

Strain Designation: MK44

NCBI tax ID(s): 2164084 (species)

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General

@ref: 65394

BacDive-ID: 158666

keywords: 16S sequence, Bacteria, aerobe, spore-forming, Gram-positive

description: Streptomyces manganisoli MK44 is an aerobe, spore-forming, Gram-positive bacterium that was isolated from soil sample of a manganese-polluted area.

NCBI tax id

  • NCBI tax id: 2164084
  • Matching level: species

doi: 10.13145/bacdive158666.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Kitasatosporales
  • family: Streptomycetaceae
  • genus: Streptomyces
  • species: Streptomyces manganisoli
  • full scientific name: Streptomyces manganisoli Mo et al. 2018

@ref: 65394

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Streptomycetales

family: Streptomycetaceae

genus: Streptomyces

species: Streptomyces manganisoli

strain designation: MK44

type strain: yes

Morphology

cell morphology

  • @ref: 65394
  • gram stain: positive

multicellular morphology

  • @ref: 65394
  • forms multicellular complex: yes
  • further description: actinomycete that forms well-developed branched substrate hyphae and aerial mycelia that differentiates into spiral spore chains consisting of oval-shaped or rod-shaped spores with smooth surfaces.

Culture and growth conditions

culture temp

  • @ref: 65394
  • growth: positive
  • type: growth
  • temperature: 10-40

culture pH

  • @ref: 65394
  • ability: positive
  • type: growth
  • pH: 5-9
  • PH range: alkaliphile

Physiology and metabolism

oxygen tolerance

  • @ref: 65394
  • oxygen tolerance: aerobe

spore formation

  • @ref: 65394
  • spore description: spiral spore chains consisting of oval-shaped or rod-shaped spores with smooth surfaces
  • spore formation: yes

halophily

  • @ref: 65394
  • salt: NaCl
  • growth: positive
  • tested relation: growth
  • concentration: 0-5 %(w/v)

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6539417057cellobiose-carbon source
6539415356cysteine-nitrogen source
6539417108D-arabinose-carbon source
6539415824D-fructose+carbon source
6539412936D-galactose+carbon source
6539416024D-mannose-carbon source
6539463150D-rhamnose-carbon source
6539416988D-ribose+carbon source
6539417924D-sorbitol-carbon source
6539427477D-valine-nitrogen source
653944853esculin-hydrolysis
653945291gelatin-hydrolysis
6539415428glycine-nitrogen source
6539416977L-alanine-nitrogen source
6539430849L-arabinose-carbon source
6539415971L-histidine-nitrogen source
6539417203L-proline-nitrogen source
6539417115L-serine-nitrogen source
6539417895L-tyrosine-nitrogen source
6539417716lactose-carbon source
6539417306maltose-carbon source
653946731melezitose+carbon source
6539417268myo-inositol+carbon source
6539417632nitrate-reduction
6539428044phenylalanine-nitrogen source
6539428017starch-carbon source
6539428017starch+hydrolysis
6539417992sucrose-carbon source
6539453423tween 40+hydrolysis
6539453426tween 80+hydrolysis
6539417151xylitol+carbon source
6539418222xylose+carbon source

antibiotic resistance

@refChEBImetaboliteis antibioticis sensitivesensitivity conc.is resistantresistance conc.
6539417833gentamicinyesyes10 µg (disc)
653942637amikacinyesyes30 µg (disc)
653947507neomycinyesyes30 µg (disc)
6539449566cloxacillinyesyes1 µg (disc)
653943745clindamycinyesyes2 µg (disc)
65394100241ciprofloxacinyesyes5 µg (disc)
653947731ofloxacinyesyes5 µg (disc)
6539428971ampicillinyesyes10 µg (disc)
65394100246norfloxacinyesyes10 µg (disc)
6539417334penicillinyesyes10 Unit (disc)
6539448923erythromycinyesyes15 µg (disc)
65394474053cefazolinyesyes30 µg (disc)
653943547cephradineyesyes30 µg (disc)
653943508ceftazidimeyesyes30 µg (disc)
6539429007ceftriaxoneyesyes30 µg (disc)
653943515cefuroximeyesyes30 µg (disc)
653943534cephalexinyesyes30 µg (disc)
6539417698chloramphenicolyesyes30 µg (disc)
6539450845doxycyclineyesyes30 µg (disc)
6539431845midecamycinyesyes30 µg (disc)
6539427902tetracyclineyesyes30 µg (disc)
6539428001vancomycinyesyes30 µg (disc)
653943493cefoperazoneyesyes75 µg (disc)
653943393carbenicillinyesyes100 µg (disc)
653948232piperacillinyesyes100 µg (disc)
653948309polymyxin byesyes300 Unit (disc)
653945195furazolidoneyesyes300 µg (disc)
653949332sulfamethoxazoleyesyes1.25 µg (disc)

metabolite production

  • @ref: 65394
  • Chebi-ID: 16136
  • metabolite: hydrogen sulfide
  • production: no

Isolation, sampling and environmental information

isolation

  • @ref: 65394
  • sample type: soil sample of a manganese-polluted area
  • geographic location: Heling town, in Xiangtan city of Hunan province
  • country: China
  • origin.country: CHN
  • continent: Asia
  • latitude: 27.8833
  • longitude: 112.75
  • enrichment culture: Gause's synthetic agar medium no. 1
  • enrichment culture duration: 7-12 days
  • enrichment culture temperature: 30
  • isolation procedure: dilution plate method on modified proline agar medium, supplemented with 2.0-3.0 ml K2Cr2O7 solution (1.775 g l-1) in a 100 ml medium to reduce fungal contamination. Then the strain was purified by restreaking on Gause's synthetic agar medium no. 1 at 30°C for 7-12 days.

isolation source categories

Cat1Cat2Cat3
#Environmental#Terrestrial#Soil
#Engineered#Contamination

Sequence information

16S sequences

  • @ref: 65394
  • description: 16S rRNA gene sequence
  • accession: KY911452
  • database: nuccore

GC content

  • @ref: 65394
  • GC-content: 75.7
  • method: thermal denaturation, midpoint method (Tm)

External links

@ref: 65394

culture collection no.: GDMCC 4.137, KCTC 39920

literature

  • topic: Phylogeny
  • Pubmed-ID: 29648527
  • title: Streptomyces manganisoli sp. nov., a novel actinomycete isolated from manganese-contaminated soil.
  • authors: Mo P, Zhao J, Li K, Tang X, Gao J
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijsem.0.002762
  • year: 2018
  • mesh: Bacterial Typing Techniques, Base Composition, China, DNA, Bacterial/genetics, Fatty Acids/chemistry, *Manganese, Mining, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, *Soil Microbiology, Soil Pollutants, Streptomyces/*classification/genetics/isolation & purification, Vitamin K 2/chemistry
  • topic2: Transcriptome

Reference

@idauthorstitledoi/urljournalpubmed
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
65394Ping Mo, Jiarong Zhao, Kaiqin Li, Xinke Tang, Jian GaoStreptomyces manganisoli sp. nov., a novel actinomycete isolated from manganese-contaminated soil10.1099/ijsem.0.002762IJSEM 68: 1890-1895 201829648527