Strain identifier

BacDive ID: 158658

Type strain: Yes

Species: Aestuariibius insulae

Strain Designation: DBTF-13

NCBI tax ID(s): 2058287 (species)

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General

@ref: 65385

BacDive-ID: 158658

keywords: 16S sequence, Bacteria, mesophilic, Gram-negative, rod-shaped, colony-forming

description: Aestuariibius insulae DBTF-13 is a mesophilic, Gram-negative, rod-shaped bacterium that forms circular colonies and was isolated from sediment sample from surface of a tidal flat.

NCBI tax id

  • NCBI tax id: 2058287
  • Matching level: species

doi: 10.13145/bacdive158658.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Alphaproteobacteria
  • order: Rhodobacterales
  • family: Roseobacteraceae
  • genus: Aestuariibius
  • species: Aestuariibius insulae
  • full scientific name: Aestuariibius insulae Park et al. 2018

@ref: 65385

domain: Bacteria

phylum: Proteobacteria

class: Alphaproteobacteria

order: Rhodobacterales

family: Rhodobacteraceae

genus: Aestuariibius

species: Aestuariibius insulae

strain designation: DBTF-13

type strain: yes

Morphology

cell morphology

@refgram staincell lengthcell widthcell shapemotility
65385negative0.4-10 µm0.2-0.8 µmrod-shapedno
65385ovoid-shaped

colony morphology

  • @ref: 65385
  • colony size: 0.5-1 mm
  • colony color: moderate orange
  • colony shape: circular
  • incubation period: 7 days
  • medium used: marine agar 2216

Culture and growth conditions

culture medium

  • @ref: 65385
  • name: Marine agar (MA)
  • growth: yes

culture temp

@refgrowthtypetemperaturerange
65385positiveoptimum25-30mesophilic
65385positivegrowth10psychrophilic
65385positivegrowth30mesophilic
65385nogrowth4psychrophilic
65385nogrowth35mesophilic

culture pH

@refabilitytypepH
65385positiveoptimum7-8
65385positivegrowth6
65385nogrowth5.5

Physiology and metabolism

spore formation

  • @ref: 65385
  • spore formation: no

halophily

@refsaltgrowthtested relationconcentration
65385NaClpositivegrowth1-6 %(w/v)
65385NaClpositiveoptimum2 %(w/v)

observation

  • @ref: 65385
  • observation: Anaerobic growth does not occur on MA and on MA supplemented with nitrate.

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6538530089acetate-carbon source
6538530089acetate+energy source
6538516150benzoate-carbon source
6538516150benzoate+energy source
65385casein-hydrolysis
6538517057cellobiose-carbon source
6538517057cellobiose+energy source
6538516947citrate-carbon source
6538516947citrate+energy source
6538515824D-fructose-carbon source
6538515824D-fructose+energy source
6538512936D-galactose-carbon source
6538512936D-galactose+energy source
6538517634D-glucose-carbon source
6538517634D-glucose+energy source
6538516024D-mannose-carbon source
6538516024D-mannose+energy source
6538565327D-xylose-carbon source
6538565327D-xylose+energy source
653854853esculin-hydrolysis
6538515740formate-carbon source
6538515740formate+energy source
653855291gelatin-hydrolysis
6538517368hypoxanthine+hydrolysis
6538530849L-arabinose-carbon source
6538530849L-arabinose+energy source
6538515589L-malate-carbon source
6538515589L-malate+energy source
6538517895L-tyrosine-hydrolysis
6538518420magnesium(2+)+required for growth
6538517306maltose-carbon source
6538517306maltose+energy source
6538517632nitrate+reduction
6538515361pyruvate-carbon source
6538515361pyruvate+energy source
6538517814salicin-carbon source
6538517814salicin+energy source
6538564243sodium L-glutamate-carbon source
6538564243sodium L-glutamate+energy source
6538528017starch-hydrolysis
6538530031succinate-carbon source
6538530031succinate+energy source
6538517992sucrose-carbon source
6538517992sucrose+energy source
6538527082trehalose-carbon source
6538527082trehalose+energy source
6538553424tween 20+hydrolysis
6538553423tween 40-hydrolysis
6538553425tween 60+hydrolysis
6538553426tween 80-hydrolysis
6538516199urea-hydrolysis
6538515318xanthine+hydrolysis

antibiotic resistance

@refChEBImetaboliteis antibioticis sensitivesensitivity conc.is resistantresistance conc.
6538528368novobiocinyesyes5 µg (disc)
653853542cephalothinyesyes30 µg (disc)
6538517833gentamicinyesyes30 µg (disc)
653856104kanamycinyesyes30 µg (disc)
653857507neomycinyesyes30 µg (disc)
6538527902tetracyclineyesyes30 µg (disc)
6538517076streptomycinyesyes50 µg (disc)
653853393carbenicillinyesyes100 µg (disc)
6538517698chloramphenicolyesyes100 µg (disc)
6538528971ampicillinyesyes10 µg (disc)
653856472lincomycinyesyes15 µg (disc)
6538516869oleandomycinyesyes15 µg (disc)
6538518208penicillin gyesyes20 Unit (disc)
653858309polymyxin byesyes100 Unit (disc)

enzymes

@refvalueactivityec
65385alkaline phosphatase+3.1.3.1
65385esterase (C 4)+
65385esterase Lipase (C 8)+
65385leucine arylamidase+3.4.11.1
65385valine arylamidase+
65385beta-galactosidase+3.2.1.23
65385catalase+1.11.1.6
65385cytochrome oxidase+1.9.3.1
65385naphthol-AS-BI-phosphohydrolase+
65385lipase (C 14)-
65385cystine arylamidase-3.4.11.3
65385trypsin-3.4.21.4
65385alpha-chymotrypsin-3.4.21.1
65385acid phosphatase-3.1.3.2
65385alpha-galactosidase-3.2.1.22
65385beta-glucuronidase-3.2.1.31
65385alpha-glucosidase-3.2.1.20
65385beta-glucosidase-3.2.1.21
65385N-acetyl-beta-glucosaminidase-3.2.1.52
65385alpha-mannosidase-3.2.1.24
65385alpha-fucosidase-3.2.1.51

Isolation, sampling and environmental information

isolation

  • @ref: 65385
  • sample type: sediment sample from surface of a tidal flat
  • geographic location: Daebu island on the Yellow Sea
  • country: Republic of Korea
  • origin.country: KOR
  • continent: Asia
  • enrichment culture: marine agar 2216
  • enrichment culture duration: 25
  • isolation procedure: standard dilution technique

isolation source categories

Cat1Cat2Cat3
#Environmental#Aquatic#Sediment
#Environmental#Terrestrial#Tidal flat

Sequence information

16S sequences

  • @ref: 65385
  • description: 16S rRNA gene sequence
  • accession: MG641160
  • database: nuccore

GC content

  • @ref: 65385
  • GC-content: 61.6
  • method: high performance liquid chromatography (HPLC)

External links

@ref: 65385

culture collection no.: KACC 19432, NBRC 113038

literature

  • topic: Phylogeny
  • Pubmed-ID: 29504921
  • title: Aestuariibius insulae gen. nov., sp. nov., isolated from a tidal flat sediment.
  • authors: Park S, Choi J, Won SM, Park JM, Yoon JH
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijsem.0.002679
  • year: 2018
  • mesh: Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Fatty Acids/chemistry, Geologic Sediments/*microbiology, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Republic of Korea, Rhodobacteraceae/*classification/genetics/isolation & purification, Seawater/*microbiology, Sequence Analysis, DNA, Ubiquinone/analogs & derivatives/chemistry
  • topic2: Transcriptome

Reference

@idauthorstitledoi/urljournalpubmed
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
65385Sooyeon Park, Jeehyun Choi, Sung-Min Won, Ji-Min Park, Jung-Hoon YoonAestuariibius insulae gen. nov., sp. nov., isolated from a tidal flat sediment10.1099/ijsem.0.002679IJSEM 68: 1350-1355 201829504921