Strain identifier
BacDive ID: 158647
Type strain: 
Species: Methylomusa anaerophila
Strain Designation: MMFC1
Strain history: <- Kazuya Watanabe, Tokyo Univ. of Pharmacy and Life Science
NCBI tax ID(s): 1930071 (species)
version 8.1 (current version)
General
@ref: 65372
BacDive-ID: 158647
keywords: genome sequence, 16S sequence, Bacteria, anaerobe, spore-forming, mesophilic, Gram-negative, motile, rod-shaped
description: Methylomusa anaerophila MMFC1 is an anaerobe, spore-forming, mesophilic bacterium that was isolated from microbial fuel cell inoculated with sludge obtained from a chemical plant.
NCBI tax id
- NCBI tax id: 1930071
- Matching level: species
strain history
| @ref | history |
|---|---|
| 67770 | N. Amano; Tokyo Univ. of Pharm. & Life Sci., Japan; MMFC1. |
| 67771 | <- Kazuya Watanabe, Tokyo Univ. of Pharmacy and Life Science |
doi: 10.13145/bacdive158647.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/bacillota
- domain: Bacteria
- phylum: Bacillota
- class: Negativicutes
- order: Selenomonadales
- family: Sporomusaceae
- genus: Methylomusa
- species: Methylomusa anaerophila
- full scientific name: Methylomusa anaerophila Amano et al. 2018
@ref: 65372
domain: Bacteria
phylum: Firmicutes
class: Negativicutes
order: Selenomonadales
family: Sporomusaceae
genus: Methylomusa
species: Methylomusa anaerophila
strain designation: MMFC1
type strain: yes
Morphology
cell morphology
| @ref | gram stain | cell length | cell shape | motility | flagellum arrangement | confidence |
|---|---|---|---|---|---|---|
| 65372 | negative | 1-4 µm | rod-shaped | yes | peritrichous | |
| 67771 | rod-shaped | yes | peritrichous | |||
| 67771 | curved-shaped | |||||
| 67771 | negative | |||||
| 69480 | yes | 96.135 | ||||
| 69480 | negative | 99.806 |
Culture and growth conditions
culture temp
| @ref | growth | type | temperature | range |
|---|---|---|---|---|
| 65372 | positive | growth | 30-37 | mesophilic |
| 67770 | positive | growth | 30 | mesophilic |
| 67771 | positive | growth | 30-37 | mesophilic |
culture pH
- @ref: 65372
- ability: positive
- type: growth
- pH: 5.9-6.9
Physiology and metabolism
oxygen tolerance
| @ref | oxygen tolerance | confidence |
|---|---|---|
| 67771 | anaerobe | |
| 69480 | anaerobe | 100 |
spore formation
| @ref | type of spore | spore formation | confidence |
|---|---|---|---|
| 65372 | endospore | yes | |
| 69480 | yes | 99.447 |
observation
| @ref | observation |
|---|---|
| 65372 | No cell aggregation in liquid medium. |
| 65372 | Produces acetate as the end metabolite from methanol, lactate and pyruvate. |
metabolite utilization
| @ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
|---|---|---|---|---|
| 65372 | 30089 | acetate | - | growth |
| 65372 | 22599 | arabinose | - | builds acid from |
| 65372 | 17750 | betaine | - | growth |
| 65372 | 28885 | butanol | - | growth |
| 65372 | 17057 | cellobiose | - | builds acid from |
| 65372 | 4853 | esculin | - | hydrolysis |
| 65372 | 16236 | ethanol | - | growth |
| 65372 | 28757 | fructose | - | growth |
| 65372 | 29806 | fumarate | - | growth |
| 65372 | 5291 | gelatin | - | hydrolysis |
| 65372 | 17234 | glucose | - | builds acid from |
| 65372 | 17234 | glucose | - | growth |
| 65372 | 17754 | glycerol | - | builds acid from |
| 65372 | 17754 | glycerol | - | growth |
| 65372 | 24996 | lactate | + | growth |
| 65372 | 17716 | lactose | - | builds acid from |
| 65372 | 17306 | maltose | - | builds acid from |
| 65372 | 29864 | mannitol | - | builds acid from |
| 65372 | 37684 | mannose | - | builds acid from |
| 65372 | 6731 | melezitose | - | builds acid from |
| 65372 | 17790 | methanol | + | growth |
| 65372 | 28831 | propanol | - | growth |
| 65372 | 15361 | pyruvate | + | growth |
| 65372 | 16634 | raffinose | - | builds acid from |
| 65372 | 26546 | rhamnose | - | builds acid from |
| 65372 | 17814 | salicin | - | builds acid from |
| 65372 | 15611 | sarcosine | - | growth |
| 65372 | 30911 | sorbitol | - | builds acid from |
| 65372 | 17992 | sucrose | - | builds acid from |
| 65372 | 17992 | sucrose | - | growth |
| 65372 | 27082 | trehalose | - | builds acid from |
| 65372 | 18222 | xylose | - | builds acid from |
enzymes
| @ref | value | activity | ec |
|---|---|---|---|
| 65372 | catalase | - | 1.11.1.6 |
| 65372 | cytochrome oxidase | - | 1.9.3.1 |
Isolation, sampling and environmental information
isolation
| @ref | sample type | geographic location | country | origin.country | continent | enrichment culture | enrichment culture composition | enrichment culture duration | enrichment culture temperature | isolation procedure |
|---|---|---|---|---|---|---|---|---|---|---|
| 65372 | microbial fuel cell inoculated with sludge obtained from a chemical plant | Gifu | Japan | JPN | Asia | agar plates | supplemented with 100 mM methanol (the methanol medium). Agar plates also contained Difco Bactoagar (1.5 %) and CaCO 3 (0.5 %). | 1 month | 30 | After electric output from the MFC had sufficiently increased (approximately 2 months after commencing the operation), a piece of the anode (approximately 1 cm2) was cut off and put in a test tube containing 5 ml mineral medium. After the tube was shaken using a vortex mixer, the microbial suspension was serially diluted and streaked on agar plates. The plates were placed in anaerobic bags and incubated at 30°C. The above procedures were conducted in a glove box (Bactron I; Shellab). |
| 67770 | Microbial fuel cell inoculated with sludge obtained from a chemical plant in Gifu | Japan | JPN | Asia | ||||||
| 67771 | From microbial fuel cell inoculated with sludge obtained from a chemical plant | Gifu | Japan | JPN | Asia |
isolation source categories
| Cat1 | Cat2 | Cat3 |
|---|---|---|
| #Engineered | #Industrial | #Engineered product |
| #Engineered | #Waste | #Industrial waste |
| #Engineered | #Waste | #Sewage sludge |
| #Host | #Microbial |
taxonmaps
- @ref: 69479
- File name: preview.99_132209.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_208;96_51403;97_66204;98_88431;99_132209&stattab=map
- Last taxonomy: Methylomusa anaerophila subclade
- 16S sequence: LC203074
- Sequence Identity:
- Total samples: 463
- soil counts: 104
- aquatic counts: 102
- animal counts: 247
- plant counts: 10
Sequence information
16S sequences
- @ref: 65372
- description: 16S rRNA gene sequence
- accession: LC203074
- database: nuccore
Genome sequences
| @ref | description | accession | assembly level | database | NCBI tax ID |
|---|---|---|---|---|---|
| 66792 | Methylomusa anaerophila MMFC1 | GCA_003966895 | complete | ncbi | 1930071 |
| 66792 | Methylomusa anaerophila MMFC1 | 2837211827 | complete | img | 1930071 |
GC content
- @ref: 67771
- GC-content: 46.6
- method: genome sequence analysis
Genome-based predictions
predictions
| trait | prediction | confidence | training_data |
|---|---|---|---|
| spore-forming | yes | 54 | no |
| motile | yes | 87.652 | no |
| flagellated | no | 53.488 | yes |
| gram-positive | no | 96.454 | yes |
| anaerobic | yes | 99.686 | yes |
| aerobic | no | 98.459 | yes |
| halophile | no | 95.311 | no |
| spore-forming | yes | 93.151 | yes |
| glucose-util | yes | 51.558 | yes |
| thermophile | no | 94.051 | no |
| glucose-ferment | no | 73.668 | no |
External links
@ref: 65372
culture collection no.: JCM 31821, KCTC 15592
literature
- topic: Phylogeny
- Pubmed-ID: 29458677
- title: Methylomusa anaerophila gen. nov., sp. nov., an anaerobic methanol-utilizing bacterium isolated from a microbial fuel cell.
- authors: Amano N, Yamamuro A, Miyahara M, Kouzuma A, Abe T, Watanabe K
- journal: Int J Syst Evol Microbiol
- DOI: 10.1099/ijsem.0.002635
- year: 2018
- mesh: Bacterial Typing Techniques, Base Composition, *Bioelectric Energy Sources, Bioreactors, DNA, Bacterial/genetics, Fatty Acids/chemistry, Firmicutes/*classification/genetics/isolation & purification, Japan, Methanol, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Sewage/microbiology
- topic2: Transcriptome
Reference
| @id | authors | title | doi/url | journal | pubmed |
|---|---|---|---|---|---|
| 20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | 10.1099/ijsem.0.004332 | ||
| 65372 | Nanako Amano, Ayaka Yamamuro, Morio Miyahara, Atsushi Kouzuma, Takashi Abe, Kazuya Watanabe | Methylomusa anaerophila gen. nov., sp. nov., an anaerobic methanol-utilizing bacterium isolated from a microbial fuel cell | 10.1099/ijsem.0.002635 | IJSEM 68: 1118-1122 2018 | 29458677 |
| 66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | https://diaspora-project.de/progress.html#genomes | ||
| 66794 | Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg | BRENDA, the ELIXIR core data resource in 2021: new developments and updates | 10.1093/nar/gkaa1025 | Nucleic Acids Res. 49: D498-D508 2020 | 33211880 |
| 67770 | Curators of the JCM | https://jcm.brc.riken.jp/en/ | |||
| 67771 | Curators of the KCTC | https://kctc.kribb.re.kr/En/Kctc | |||
| 69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | MicrobeAtlas 1.0 beta | https://microbeatlas.org/ | ||
| 69480 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | https://diaspora-project.de/progress.html#genomes |